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Citations for Thermo Fisher :
2251 - 2300 of 10000+ citations for Rat Corticosteroid 11 Beta Dehydrogenase Isozyme 2 HSD11B2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... All antibodies were used at a concentration of 1:11 in permeabilization medium (medium B; Invitrogen). Data was acquired using a MacsQuant VYB flow cytometer (Miltenyi ...
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bioRxiv - Cancer Biology 2019Quote: CRC datasets in the Oncomine database (www.oncomine.org, accessed 11/2014, Thermo Fisher Scientific, Ann Arbor, MI) were searched using the differential expression module for cancer vs ...
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bioRxiv - Cell Biology 2021Quote: ... 11 glucose (pH 7.4) and loaded with 20 nM TMRM or 5 μM MitoSox (Thermo Fisher) for 30 minutes at 37°C and then placed in glass-bottomed chamber (MatTek ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred onto PVDF (GE) membranes using electrophoresis at 11 V for 18 hours (Invitrogen). Because GAPDH protein changed across puberty (data not shown ...
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bioRxiv - Cell Biology 2021Quote: ... were cultured in Dulbecco’s Modified Eagle medium (DMEM; Life Technologies, Carlsbad, CA, USA, #11-995-073) with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2021Quote: ... The suspension was transferred to a pre-chilled 0.1 cm electroporation cuvette (Thermo Scientific #5510-11). A single pulse of 1.8 kV was given by a Gene Pulser Xcell® Microbial System (Bio-Rad #1652662) ...
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bioRxiv - Bioengineering 2022Quote: ... 11 and 21 days 37 °C in a Forma Steri-Cycle CO2 incubator (Thermo Scientific, UK). Post-seeding CYP1A2 and CYP2E1 mRNA expression in HepG2 2D and 3D cultures were measured relying on RT-PCR ...
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bioRxiv - Cell Biology 2022Quote: ... the primers listed in Supplementary Table 11 and a 7500 Real Time PCR System (Applied Biosystems). Gene expression levels were quantified relative to GAPDH expression using the ΔCt method ...
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bioRxiv - Systems Biology 2022Quote: ... equal amount of the 11 channels of TMT11plex label reagents (catalog number A34808, ThermoFisher Scientific Inc.) were pooled to generate a TMT mix ...
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bioRxiv - Microbiology 2019Quote: ... cells were instead incubated in thymidine-free HMI-11 media (Iscove’s Modified Dulbecco’s Medium (IMDM) (Gibco), 10% (v/v ...
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bioRxiv - Molecular Biology 2020Quote: ... 11 tilt-series of the sample were collected automatically with Tomography software (Thermo Fisher Scientific, USA) in low-dose mode with 18000x magnification (pixel size 3.7 Å ...
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bioRxiv - Molecular Biology 2020Quote: ... The fixed cells were incubated with FITC-conjugated anti-BrdU antibody (11-5071-42, RRID:AB_11042627, Invitrogen), further stained with 20 µg/mL of propidium iodide (Sigma-Aldrich ...
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bioRxiv - Immunology 2019Quote: ... CART19 cells were stained with FITC-conjugated anti-CD4 (Cat# 11-0048-41, Thermo Fisher Scientific) and PerCP-Cy5.5-conjugated anti-CD8 antibody (Cat# 344710 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... They were then heated to 100°C using a hot plate (Fisher Scientific / 11-600-49SH) and simmered for 20 min ...
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bioRxiv - Developmental Biology 2022Quote: ... Paraffin-embedded embryos/tadpoles were sectioned (11 μm) on a Microm HM 340E microtome (Thermo Scientific). EdU incorporation was detected on paraffin sections using the Click-iT EdU Imaging Kit according to manufacturer’s recommendations (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: The following qPCR probes were used for mRNA expression analyses: SYNGAP1 exon 10-11 (Hs00405348_m1, ThermoFisher), SYNGAP1 exon 16-17 (Hs.PT.58.4622325 ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 x 105 KMS-11 cells were labelled with 5 µM CellTrace™ Blue dye (Invitrogen) for 20 min at 37°C in the dark ...
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bioRxiv - Immunology 2024Quote: ... containing 11 mM [U-14C]-glucose (0.1mCi/mmol) or in RPMI 1640 medium (Thermo Fisher 11879) containing 2 mM [U-14C]-glutamine (0.1mCi/mmol) ...
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bioRxiv - Biophysics 2023Quote: ... 32 μM each of dGTP/biotin-14-dATP/biotin-11-dUTP/biotin-14-dCTP (Thermo Fisher), and 5 U Klenow in 1× NEBuffer 2 (New England BioLabs ...
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bioRxiv - Genomics 2023Quote: ... optical absorption spectra (DS-11, DeNovix, Willmington, DE, USA) and size profile (Fragment Analyzer, Thermo Fisher).
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bioRxiv - Systems Biology 2023Quote: ... The digested peptides were labeled with 11-plex TMT reagents following the manufacturer’s instructions (Thermo Scientific). MP was labeled by TMT channel 131C ...
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bioRxiv - Immunology 2022Quote: ... They were both determined to be of the IgG2a subclass by using the Pro-DetectTM Rapid Antibody Isotyping Kit-Rat (Thermo Fisher Scientific).
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bioRxiv - Immunology 2022Quote: ... The supernatants of human CD19 CAR T cells or human TCR transfected CD8+ T cells were analyzed by IFNγ Human Uncoated Elisa Kit (Invitrogen, Thermo Fisher Scientific # 88-7316-88). Plates were read on BioRad plate reader at a wavelength 450 nm and subtracted by 595 nm.
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bioRxiv - Microbiology 2020Quote: ... Supernatants were collected for analysis in an IL-12/IL-23 p40 (total) mouse uncoated ELISA kit (No. 88-7120-22; Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Immunology 2024Quote: The amounts of TNF-α and IL-6 in culture supernatants of LPS or Pam3cys-re-stimulated IS-trained macrophages were quantified using commercial human ELISA kits (Thermo Fisher Scientific, Waltham, MA, USA). Optical density was measured using the Infinite M200 (Tecan ...
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bioRxiv - Cancer Biology 2023Quote: ... CM was generated as mentioned above and GM-CSF was quantified using the mouse GM-CSF uncoated ELISA Kit (Thermo Fisher, cat.no. #88-7334-22) in accordance with the protocol provided by the manufacturer ...
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bioRxiv - Immunology 2021Quote: ... We generatead F(ab’)2 fragments using Pierce™ Mouse IgG1 Fab and F(ab’)2 Preparation Kit (ThermoFisher Scientific #44980). ROS production upon stimulation with or without 10 μM peptide S100A6-1 coated to a Microfluor® 2 white-bottom 96-well plate (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: T or B cells (2-4 × 107) obtained from 2 spleens of actin-GFP mice by negative MACS kits (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Organoid proliferation used the thymidine analogue 5-ethynyl-2’-deoxyuridine (EdU) for 2 hrs using Click-iT EdU Alexa Fluor-555 Imaging Kit (Invitrogen, C10338). H&E and IHC slides were viewed by brightfield-fluorescent microscopy (Olympus BX60) ...
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bioRxiv - Immunology 2023Quote: ... the appropriate secondary antibodies were added diluted in 1% BSA in PBS for 1 hour at a 1:1000 dilution (Goat anti Rat 488 Thermo Fisher Scientific a11006, Goat anti Rat 647 Thermo Fisher Scientific a21247 ...
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bioRxiv - Microbiology 2023Quote: ... Lysates and immunecomplexes were separated by SDS-PAGE and blotted to detect either biotynlated proteins (IRDye® 680LT streptavidin (Li-Cor)) or CDPK3HA (rat-anti-HA)/anti-rat DyLight™ 800 (Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... Alexa488 anti-rat and Cy3 anti-rat (1:250, Jackson) secondary antibodies or with Alexa647-conjugated Streptavidin (1:200, Thermo Fisher Scientific) and by counterstaining with DAPI (0.2 µg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... sera samples and conditioned tissue culture medium from rat hepatic stellate cells (HSCs) or primary rat hepatocytes (PCs) were stained with phycoerythrin (PE)-labeled Cell Mask (Thermo Fisher Scientific) or stained with Syto RNASelect (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2019Quote: ... The reaction was quenched by adding 5mM beta-mercaptoethanol and desalted to remove unreacted dye (ThermoFisher, 7K MWCO). The extent of labelling was quantified by measuring the 595nm/280nm absorbance ratio ...
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bioRxiv - Biochemistry 2021Quote: ... for spike protein or RBD detection and mouse anti-beta tubulin antibody (1:5000; Thermo Fisher; MA5-16308) as a loading control ...
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bioRxiv - Neuroscience 2021Quote: ... The reaction was stopped following the addition of 4x sample buffer (containing 10% beta mercaptoethanol (BME; Thermo Fisher)) to each well and heated to 95°C for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... Western blotting for GATA3 and Beta-Actin was carried out by lysing the cells with RIPA buffer (ThermoFisher) supplemented with benzonase and protease inhibitors ...
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bioRxiv - Cancer Biology 2023Quote: ... Treatment with the vehicle hydroxypropyl-beta-cyclodextrin (HPBCD, Acros Organics; 5% w/w in water for injection (WFI)) ...
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bioRxiv - Cell Biology 2024Quote: ... the phogrin-Dronpa-Green1 cDNA expression vector was transduced to primary beta cells using ViraPower adenovirus system (Invitrogen). The cells were then fixed in 4% paraformaldehyde/PBS for 10 min and observed using a TIRF/PALM microscope (ZEISS) ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was incubated with anti-beta Actin primary antibody (Thermo Fisher Scientific, PA5-85271, 1:5000 dilution) at 4°C overnight in gentle agitation ...
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bioRxiv - Genetics 2021Quote: ... The solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) (Thermo Fisher, EH2IL2) was used to measure IL2 concentration from cell supernatant according to the manufacturer’s protocol(https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0014639_EH2IL2_ELISA_Kit_PI.pdf) ...
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bioRxiv - Immunology 2020Quote: ... perfringens native ECP or native CWP were coated on ELISA plates (Nunc MaxisorpTM ...
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bioRxiv - Molecular Biology 2021Quote: ... 80 μL/well diluent SuperSignal Elisa Femto Maximum Sensitivity Substrate (ThermoFisher Scientific) was added ...
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bioRxiv - Immunology 2020Quote: ... Plates were developed using 1-Step Ultra TMB-ELISA (34028, Thermo Fisher) for one to two minutes after which the reaction was stopped with 1M H2SO4 ...
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bioRxiv - Biophysics 2019Quote: ... 300 μL of SuperSignal ELISA Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific) were added and the luminescence was measured using a Glomax 20/20 Luminometer (Promega ...
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bioRxiv - Immunology 2019Quote: ... overnight at 4C on Nunc Maxisorp 96-well ELISA plates (Thermo Scientific). Wells were washed once with PBS and 1×105 CAR+ T cells were plated in 200uL CM and cultured for 24h ...
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bioRxiv - Microbiology 2021Quote: PPS3 ELISAs were performed using 96-well Nunc Maxisorp plates (Thermofisher Scientific) coated with purified PPS3 (ATCC ...
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bioRxiv - Biochemistry 2020Quote: ... and developed with 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Fisher) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... Plates were developed using SuperSignal™ ELISA Femto Maximum Sensitivity Substrate (ThermoFisher) and read at 425 nm ...
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bioRxiv - Microbiology 2020Quote: ... ELISA was performed as follows: 96-well MaxiSorp plates (NUNC, Roskilde, Denmark) were coated with 100 ng/well of mouse anti-Penta His BSA-free antibody (Qiagen ...