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Citations for Thermo Fisher :
2251 - 2300 of 10000+ citations for Probable U3 small nucleolar RNA associated protein 11 UTP11 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... the S protein labelling was visualized with an Alexa-488-conjugated goat anti-human antibody (ThermoFisher, cat. No.: A11013), while for the N protein labeling an Alexa-488-conjugated goat anti-rabbit secondary antibody (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... while for the N protein labeling an Alexa-488-conjugated goat anti-rabbit secondary antibody (ThermoFisher, cat. No.: A11008) was used ...
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bioRxiv - Physiology 2024Quote: ... Proteins were transferred to nitrocellulose membranes and incubated with primary antibodies directed against Kcne1 (PA5-106872, Thermo Fisher Scientific) and β-Actin (A5441 ...
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bioRxiv - Molecular Biology 2024Quote: ... whole cell extracts were prepared and mixed with each antibody conjugated with Dynabeads Protein A (Invitrogen–Thermo Fisher Scientific). After culturing for 48 h ...
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bioRxiv - Neuroscience 2024Quote: ... Both recombinant proteins were pre-clustered with 10 µg/mL of an anti-human IgG antibody (Thermo Fisher, U.S.A.). Cortical single cells were fixated with 4% PFA/1x PBS for 10 min.
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bioRxiv - Cancer Biology 2024Quote: ... Dynabeads Protein A were washed and blocked in ice-cold BSA-PBS (0.5%) before incubation with MSH6 antibody (ThermoFisher) alongside an IgG control overnight at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... unspecific binding of primary antibodies was blocked by incubating the embryos in Ultra Vision Protein block (Thermo Fisher Scientific) for 10 minutes at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... Lungs were cut into small pieces on ice in a solution containing collagenase IV (160 U/mL, Gibco cat.17104019)/dispase (0.6 U/mL ...
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bioRxiv - Cell Biology 2021Quote: ... The conjunction tissue was cut into small fragments and cultured in 75 mm plate with Dulbecco’s modified eagle medium (DMEM, Thermo Fisher) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... with small modifications after the addition of the mitochondrial membrane potential MitoTracker® Deep Red FM fluorescent dye (Invitrogen no.M22426) at 0.6 μM to identify live parasites (Supplementary Fig ...
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bioRxiv - Immunology 2022Quote: Two-millimeter tissue sections were excised 5 cm from the distal end of the small intestine (ileum) and stored in RNAlater (ThermoFisher) overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... or liver tissue was minced with scissors into small pieces and placed in 5ml of collagenase buffer (1x HBSS w/ calcium and magnesium (Gibco), 1 mg/ml Collagenase A (Roche ...
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bioRxiv - Microbiology 2022Quote: ... WTC hiPSCc were passaged as small clumps for maintenance or single cell-like suspension for cardiac differentiation using Versene (Gibco) and mTeSR Plus supplemented with 10 μM Y-27632 (Tocris) ...
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bioRxiv - Cell Biology 2022Quote: ... mature human small intestinal colonic organoids grown with the expansion media were digested into single cells using TrypLE Express (ThermoFisher) and seeded into Matrigel-coated 96-well culture plate (Corning ...
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bioRxiv - Cancer Biology 2022Quote: ... mechanically dissociated into small fragments using narrow aperture glass pipettes then digested briefly for 5 min at 37°C in TrypLE express (Gibco) which was then quenched using DMEM (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: Tumor tissues (fresh or frozen) were minced into small pieces using surgical blade and scalpel (08-957-5D, Fisher Scientific) and processed using the tumor dissociation kit (130-096-730 ...
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bioRxiv - Cell Biology 2021Quote: ... perilimbal conjunctival tissue was minced into small fragments (~ 1mm2) and treated with 0.05% trypsin-EDTA solution (Thermo Fisher Scientific, USA) for 20 minutes at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... A small aliquot of each cell suspension was mixed with the same volume of Trypan Blue Solution (0.4%) (Thermo Fisher) to estimate cell concentration and cell viability before loading the cell preparations into the Chromium chip (10x Genomics).
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bioRxiv - Cancer Biology 2022Quote: ... Livers were weighted and 50% of the tissue was sliced in small pieces and incubated 30 minutes at 37°C in 10 ml of digestion medium (RPMI GlutaMAX medium [Gibco] containing 200U/ml of collagenase type IV [Sigma-Aldrich] and 100 U/ml of DNAse I [Sigma-Aldrich]) ...
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bioRxiv - Cancer Biology 2020Quote: ... in murine small intestinal organoids medium containing advanced DMEM/F12 medium (adDMEM/F12; Thermo Fisher Scientific, cat. no. 12634-010), GlutaMAX 1% (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... Samples on cover glass were gently mounted upside-down with a small drop of ProLong™ Gold (Invitrogen™ P36930). smFISH imaging was performed on a Leica THUNDER Imager 3D Cell Imaging system ...
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bioRxiv - Immunology 2021Quote: from PyMT or wild-type littermate control were cut in small pieces and digested for 1h at 37°C in CO2-independent medium (Gibco) containing 150 μg/mL DNAse and 75 μg/mL Liberase TL (Roche) ...
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bioRxiv - Developmental Biology 2020Quote: Mouse small intestinal organoids derived from the duodenum of C57BL/6 mice were cultured in ENR medium: Advanced DMEM/F12 (Invitrogen) with 1% Penicillin/Streptomycin (P/S ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were grown in RPMI-1640 without phenol red with 10% FBS that has been dialyzed to remove small molecules (Gibco, 26400044 ...
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bioRxiv - Immunology 2020Quote: ... Lung leukocytes were cut in small pieces and cells were further isolated by an incubation in 300IU/ ml collagenase II (Gibco) and 3μg/ml DNase I (Roche ...
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Characterization of a novel Fgf10CreERT2 knock-in mouse line targeting postnatal lung Fgf10 lineagesbioRxiv - Developmental Biology 2021Quote: ... Then using sharp blades to cut the lung into small pieces and digested with 0.5% collagenase Type IV in HBSS (Life Technologies, Invitrogen) for 45 minutes at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... The solution was then replaced with ultrasound gel (HealthCare Plus) and a small cover slip (5 mm diameter; Fisher Scientific) was placed on top ...
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bioRxiv - Immunology 2023Quote: ... Small intestine was open longitudinally and feces and mucus were removed using PBS and tissues were cut in small pieces of approximately 2 cm and transfer to a 50 mL tube with HBSS no Ca2+ or Mg2+ (Gibco) supplemented with 10% FCS and 2 mM EDTA ...
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bioRxiv - Immunology 2023Quote: ... excised 4cm2 skin sections were diced into small pieces with a scalpel blade and enzymatically digested with Collagenase type I (500 U/ml; Gibco) and DNAse I (1 mg/ml ...
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bioRxiv - Neuroscience 2023Quote: Small molecule leakage into the brain was assessed by EZ-Link Sulfo-NHS-Biotin (MW = 443 kDa, Thermo Fisher Scientific). Briefly ...
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bioRxiv - Biophysics 2023Quote: ... we transferred the fish to a small tank filled with 7L of tank water with buffered MS-222 (Acros Organics) solution (0.02% ...
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bioRxiv - Cancer Biology 2023Quote: ... and non-small-cell lung carcinoma (NSCLC) cell lines H661 and H1299 were cultured in filter-sterilized RPMI medium (Gibco) with 10% FBS (Hyclone GE Healthcare) ...
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bioRxiv - Developmental Biology 2022Quote: ... crypt-enriched and dissociated to a single cell suspension as described previously.23 Developmental samples were dissociated by mincing whole small intestinal tissues in 1x TrypLE-express digestion reagent (Gibco) supplemented with 50ug/mL DNAseI (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... Flow analysis for small molecule inhibitor treatments in HEK293T cells were performed in the Attune NxT Flow Cytometry System (ThermoFisher). For all assays with the stable cell lines generated using the pLenti6.2-ORF-mClover2-P2A-mRFP vector and all experiments with the DNAJA1 and DNAJA2 KOs ...
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bioRxiv - Biochemistry 2024Quote: Buffers used for stopped flow experiments were decalcified by passage over a small column of Calcium Sponge (Molecular Probes/ThermoFisher). Indo-1 was used to determine the effectiveness of decalcification ...
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bioRxiv - Biochemistry 2024Quote: Buffers used for stopped flow experiments were decalcified by passage over a small column of Calcium Sponge (Molecular Probes/ThermoFisher). Indo-1 was used to determine the effectiveness of decalcification ...
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bioRxiv - Cell Biology 2024Quote: ... The peptide extracts are reduced in volume by vacuum centrifugation and a small portion of the extract is used for fluorometric peptide quantification (ThermoFisher). One microgram of sample based on the fluorometric peptide assay was loaded for each LC/MS/MS analysis.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... fresh right lung of WT and Trem2-/- mice was minced into small pieces with scissors and digested in RPMI 1640 culture medium (Gibco) with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... The epidermis was peeled off and discarded and the dermis was minced into small pieces and digested at 37°C for 1 h following 4°C for overnight using collagenase IV (17104019, Gibco). The resulting cell suspension was filtered through a 70 μm cell strainer (BD Falcon) ...
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bioRxiv - Cell Biology 2023Quote: ... the kidneys from one mouse were harvested and minced into small pieces (∼1 mm3) in Hanks’ Balanced Salt Solution (HBSS, #2323615, Gibco, Thermo Fisher Scientific Inc. ...
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bioRxiv - Cell Biology 2022Quote: ... mandibular alveolar bone tissue was cut into small pieces (<1 mm3) and digested with 3 mg/mL collagenase type I (Gibco) and 4 g/mL dispase II (Sigma ...
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bioRxiv - Immunology 2022Quote: ... with surrounding necrotic areas being carefully removed and were cut into small pieces of 2–4 mm and reserved in the mixture of 1X DMEM medium (Gibco) and Penicillin-Streptomycin solution (Hyclone) ...
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bioRxiv - Immunology 2022Quote: ... cut into small pieces and digested with collagenase D and DNase I for 1 h at 37 °C in HBSS (Gibco). After digestion ...
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bioRxiv - Immunology 2022Quote: ... RNA (15ng) isolated from small intestinal crypts was retro-transcribed to cDNA using SuperScript VILO cDNA Synthesis Kit (11754-05; Invitrogen). Barcoded libraries were prepared using the Ion AmpliSeq Transcriptome Mouse Gene Expression Panel ...
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bioRxiv - Neuroscience 2022Quote: Larval fish (4-7 dpf) were mounted in a small drop of 2% low melting point agarose (ThermoFisher Scientific 16520) in the center of the uncoated side of a mirror galvanometer (Thorlabs GVS0111) ...
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bioRxiv - Cell Biology 2023Quote: Unirradiated and irradiated mice were sacrificed at indicated time points and the middle 10 to 12 cm length (jejunum) of the small intestine was isolated and flushed with cold DPBS (Gibco). The tissue was then divided into 2 cm segments for 10% formalin fixation for 16-24 hours ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... The embryonic body was minced using sterile forceps and small pieces of tissue were transferred to 5 mL Trypsin-EDTA (0.05%; Gibco #25300054) and homogenized through repeated pipetting ...
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bioRxiv - Microbiology 2024Quote: ... A small amount of the mixture was stained with trypan blue stain 0.4% (Gibco®, Thermo Fisher Scientific Inc., USA) for observation and enumeration under a light microscope.
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bioRxiv - Immunology 2024Quote: ... 2 cm section of small bowel tissue was excised approximately 1 cm from the cecum and frozen at -80°C in Trizol reagent (Invitrogen). To extract RNA ...
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bioRxiv - Neuroscience 2024Quote: ... at ZT14 were anesthetized on ice in the dark followed by dissections in small batches in ice-cold Schneider’s Drosophila medium (SM, Gibco #21720001). About 80-100 brains were dissected for each genotype and incubated in an enzyme solution containing 0.75ug/ul Collagenase (Sigma-Aldrich ...