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Citations for Thermo Fisher :
2251 - 2300 of 8794 citations for IL 13 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 Flp-In T-Rex cells were maintained in DMEM supplemented with 10% tetracyclin-free fetal bovine serum (Gibco, 10082139) and 1% penicillin/streptomycin (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... 15 × 104 wildtype HEK293 cells or SMAP1 KO A2 cells were transfected with GFP-vWF with Lipofectamine LTX (Thermo Fisher) by reverse transfection ...
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bioRxiv - Physiology 2020Quote: HEK293 cells were purchased from ATCC (Manassas, VA) and incubated in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Grand Island, NY) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... HEK293 and CHO cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12; Gibco, #11320033), supplemented with 10% (w/v ...
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bioRxiv - Microbiology 2021Quote: ... Transfections were carried out using 1 mg of total DNA (500 μg each of VH and Vκ plasmid DNA) and 1 l of cultured HEK293-F cell suspension maintained in sterile Freestyle 293 expression medium (Invitrogen) without antibiotics at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... The mixtures were kept still for 10 min and then added separately to a 24-well plate with each well containing 1mL of 106/mL HEK293-Freestyle cells (Invitrogen). The transfected cells were cultured by shaking at a speed of 200 rpm at 37 °C for 72 h followed the transfection ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293 cells were seeded at ∼10-15 % confluency 48 hours before a triple transfection with Lipofectamine 2000 (Thermo Fisher Scientific) as per the manufacturers protocol.
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bioRxiv - Systems Biology 2021Quote: ... HEK293 transfected cells were stained with Hoechst 3342 nuclear stain and a wheat germ agglutinin (WGA, Invitrogen Alexa Fluor 647) plasma membrane (PM ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293 cells were obtained from ATCC (CRL-1573) and cultured in DMEM with Glutamax and 25 mM glucose (Life Technologies) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Neuroscience 2021Quote: HEK293 HeLa and M17 cells were cultured at 95% air and 5% CO2 in DMEM (for HEK293 and HeLa) or DMEM-F12 (1:1; for M17) supplemented with 10% fetal bovine serum (Gibco) and penicillin-streptomycin (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: HEK293 were transfected with 3xFlag-tagged plasmids encoding each SARS-CoV-2 protein by Lipofectamine 3000 (Thermo Fisher Scientific, #L3000015). 3×107 HEK293 cells were harvested in 10 ml lysis buffer (50 mM Tris-HCl [pH 7.5] ...
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Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying essential circRNAsbioRxiv - Molecular Biology 2020Quote: ... The lentivirus of gRNA or shRNA library was produced by co-transfection of library plasmids with two viral packaging plasmids psPAX and pMD2.G into HEK293 cells using Lipofactamine 2000 (Invitrogen). Huh7 cells were transduced with lentivirus libraries at multiplicity of infection (MOI ...
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bioRxiv - Microbiology 2020Quote: ... and HEK293S GnTI-/- cells (ATCC CRL-3022) in Freestyle 293 Expression Medium supplemented with 2% ultra-low IgG FBS (Gibco). We maintained ...
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bioRxiv - Biophysics 2022Quote: ... CaVβ3-stable and HEK293S GnTI-cells were grown in suspension at 37°C supplied with 8% CO2 in FreeStyle 293 Expression Medium (Gibco) supplemented with 2% fetal bovine serum (FBS ...
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bioRxiv - Genomics 2022Quote: HEK293 cells were obtained from ATCC (CRL-1573) and grown in DMEM (high glucose, L-glutamine, no sodium pyruvate) (ThermoFisher), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Molecular Biology 2023Quote: ... The 3’ ends of the sperm and HEK293 TFAM mRNA species were mapped by 3’ RLM-RACE using the FirstChoice RLM-RACE Kit (Ambion) with modifications ...
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bioRxiv - Molecular Biology 2022Quote: ... fused to a C-terminal trimeric GCN4 (tGNC4) zipper tag via a 5XGGS linker region was secreted from suspension HEK293 FreeStyle cells (ThermoFisher) in the presence of 20 μM kifunensine (Dextra ...
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bioRxiv - Cell Biology 2023Quote: ... Lentivirus was generated according to the manufacturer’s specifications by co-transfecting HEK293-FT’s with a mixture of packaging plasmids (ViraPower Lentivirus Expression System; ThermoFisher #K497500). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... HeLa (ATCC CCL-2) and HEK293 (ATCC CRL-1573) cell lines were maintained in Dulbecco’s Modified Eagle Medium (Invitrogen 11965092) supplemented with 10% fetal bovine serum (Invitrogen 16140071) ...
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bioRxiv - Neuroscience 2023Quote: ... fusion protein was transfected in human embryonic kidney (HEK293) cells in a 6-well format with or without full-length wild-type BACE1 using Lipofectamine 2000 (Invitrogen). After 6-8h transfection ...
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bioRxiv - Microbiology 2023Quote: ... suspension-adapted and serum-free HEK293S cells were seeded at 3 × 106 cells/mL in Freestyle-293 medium (ThermoFisher Scientific). Next ...
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bioRxiv - Biochemistry 2023Quote: ... To generate stable expression cell lines Flp-In T-Rex HEK293 were grown in 100 μg/ml Zeocin (Invitrogen, R25001) for at least a week before transfection ...
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bioRxiv - Biochemistry 2023Quote: ... PANX1 and its mutants and PANX3 were transiently transfected into HEK293 cells with an enhanced green fluorescent protein (eGFP) using the transfection agent Lipofectamine 2000 (Invitrogen). The cells expressing GFP were selected for patch clamp electrophysiological recordings after 24-36 hours of transfection.
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells (a gift from H. Choe) used for generation of lentiviral particles were maintained in DMEM (Gibco #11995-065) supplemented with 10% fetal bovine serum (Sigma F0926 ...
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bioRxiv - Neuroscience 2023Quote: ... fusion protein was transfected in human embryonic kidney (HEK293) cells in a 6-well format with or without full-length wild-type BACE1 using Lipofectamine 2000 (Invitrogen) as described previously (31) ...
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bioRxiv - Molecular Biology 2023Quote: ... and ATG13 KO GFP-LC3B HEK293 cells (a kind gift from O. Florey) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; GIBCO Life Technologies) containing 10% Fetal Calf Serum (FCS) ...
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bioRxiv - Biochemistry 2023Quote: U2 OS and HEK293-T cells were grown at 37 °C in 5 % CO2 in DMEM low glucose (Gibco 11885) supplemented with 10 % heat inactivated FBS (F4135 ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting constructs were integrated into HEK293 Flp-In T-REx cells using the Flp-In technology (Thermo Fisher Scientific). HEK293 Flp-In T-REx were seeded in 6-well plates and co-transfected the following day with 200 ng bait-BirA*-FLAG construct and 2 μg pOG44 (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 cells stably expressing N-terminally signal sequence FLAG (ssf)-tagged µOR (HEK293-µOR) were cultured in the presence of 250 μg/ml Geneticin (Gibco). For transient DNA expression ...
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bioRxiv - Biophysics 2023Quote: ... HEK293S GnTI- suspension cells (ATCC CRL-3022, lot number 62430067) were cultured at 37 °C in Freestyle 293 medium (Gibco) supplemented with 2% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... ASAP3 construct was cloned in pcDNA3.1/Puro-CAG plasmid backbone and used to transfect HEK293 cells with Lipofectamine 3000 (ThermoFisher, US) using 400 ng DNA ...
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bioRxiv - Immunology 2023Quote: ... non-transfected HEK293 cells were labeled with the CFSE CellTrace and the Soromba expressing cells were labeled with Violet CellTrace (Invitrogen). After washing ...
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bioRxiv - Biochemistry 2024Quote: ... plasmid DNA into 1 × 106 HEK293 cells plated per well of a 6-well plate using Lipofectamine 2000 transfection reagent (Invitrogen). Each well was transfected with 2 µg of total plasmid DNA (50 ng Cg4-puro ...
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bioRxiv - Microbiology 2020Quote: ... we used human monoclonal antibodies produced recombinantly in human Expi293F cells (Life Technologies) as described before (Fang et al ...
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bioRxiv - Cell Biology 2021Quote: ... anti-human IgG-Alexa647 and anti-human IgG-Alexa568 were obtained from Invitrogen, anti-acetylated tubulin (T7451 ...
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bioRxiv - Cell Biology 2020Quote: ... and human GAPDH and human KIF18A Taqman probes and primers (Thermo Fisher Scientific) were used for reverse transcription and qRT-PCR ...
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bioRxiv - Neuroscience 2020Quote: ... and subjected to human total tau ELISA (human tau: # KHB0042, Thermo Fisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... Supernatants were collected for analysis in an IL-12/IL-23 p40 (total) mouse uncoated ELISA kit (No. 88-7120-22; Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Immunology 2021Quote: ... then reverse transcribed into cDNA using a high-capacity cDNA reverse transcription kit (Thermo Fisher, 43-688-13). RNA levels were quantified using the IDT Prime Time Gene Expression Master Mix ...
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bioRxiv - Neuroscience 2021Quote: ... The custom heat block was attached to a temperature-controlled recirculating water bath (13-874-180, Fisher Scientific) set to the cultivation temperature ...
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bioRxiv - Microbiology 2019Quote: ... Ribosomal RNA (rRNA) subtraction on 8 of the 13 biofilm samples was done using the MicrobExpress kit (Ambion). Good quality RNA (RIN > 7 ...
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bioRxiv - Molecular Biology 2019Quote: ... Aliquots were solubilized in 1 mL 8M urea lysis buffer: 8M Urea (Invitrogen, CA; CAS# 57-13-6), 100mM NaCl(Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Then sections were incubated in primary antibody solution (GFAP, 1:1000, Life technologies, 13-0300, Rat monoclonal antibody) overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... Corning™ Accutase™ detachment solution and phorbol 12-myristate 13-acetate (PMA) were obtained from Fisher Scientific (cat ...
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bioRxiv - Cancer Biology 2020Quote: ... BT-12 and BT-13[7] were cultured in Dulbecco’s modified Eagle’s medium with Nutrient Mixture F-12 (DMEM/F12, Gibco) supplemented with 2 mM L-glutamine ...
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bioRxiv - Molecular Biology 2022Quote: ... INS-1 832/13 cells were cultured in 11 mM glucose RPMI 1640 (Thermo Fisher Scientific, Cat# 11875) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Evolutionary Biology 2022Quote: ... cDNA libraries were amplified using 12-13 PCR cycles and quantified on a Qubit Fluorometer (Thermo Fisher Scientific). Average fragment size was determined on a Fragment Analyzer (Agilent) ...
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bioRxiv - Genomics 2022Quote: ... and a 1:100 dilution of Anti-mouse CD34 biotinylated antibody (Thermo Fisher Scientific, Cat#: 13-0341-85) for 20min at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 4T1 and J774.2 cells were seeded on 13-mm Nunc™ Thermanox™ Coverslips (ThermoFisher Scientific, Carlsbad, CA) in a 24-well plate at a density of 60,000 and 100,000 cells/well respectively ...
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bioRxiv - Cancer Biology 2021Quote: ... or AA-free DMEM (US Biologicals #D9800-13) without or with 10% dialyzed FBS (Life Technologies #A33820-01) for 50 minutes ...