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Citations for Thermo Fisher :
2251 - 2300 of 10000+ citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The resin was removed by vacuum filtration and the ‘one ring’ macrocyclic peptides precipitated with cold diethyl ether (50 mL) and by centrifugation at 4000 g for 5 min at 4 ◦C on a Heraeus Multifuge X1R centrifuge (Thermo Fisher Scientific, Waltham, MA, USA) or Sigma 4-16KS centrifuge (Merck ...
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bioRxiv - Biochemistry 2024Quote: ... The resin was removed by vacuum filtration and the peptides were precipitated with cold diethyl ether (50 mL) and centrifugation at 4000 g for 5 min at 4 ◦C either on a Heraeus Multifuge X1R centrifuge (Thermo Fisher Scientific, Waltham, MA, USA) or a Sigma 4-16KS centrifuge (Merck ...
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bioRxiv - Biophysics 2020Quote: ... Samples were heated to 90°C over 1 hr and then slowly cooled overnight to 4 °C (−1°C per 20 minutes) using a Thermocycler (ProFlex PCR System, ThermoFisher). Refolded samples were resuspended through pipetting and combined into a fresh 1.5 mL microfuge tube ...
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bioRxiv - Cancer Biology 2022Quote: ... washed with PBS and pelleted by centrifugation (450 x g, 10 min, 4 °C) prior to being stored at -80 °C in TRIzol Reagent (Invitrogen). RNA was extracted with the Direct-zol RNA Microprep kit as per manufacturer’s instructions (Zymo Research R2062) ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated with primary antibodies in 10% NGS/NDS in PBST over night at 4°C following 1h incubation at 24°C with appropriate Alexa488/568 coupled secondary antibodies (1:1000, Invitrogen). Cell nuclei were stained with DAPI and final images were acquired using Leica TCS SP8 confocal microscope (Leica Microsystems ...
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bioRxiv - Synthetic Biology 2020Quote: ... Treated (or mock treated) cells were then incubated at 4°C or 37°C for >60 min in Fluorobrite (Gibco) culture medium ...
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bioRxiv - Cell Biology 2021Quote: WBM cells were incubated for 30 minutes at 4°C with antibodies for HSC isolation (CD34, CD48, Sca, c-Kit; Invitrogen), lineage markers (CD3e ...
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bioRxiv - Neuroscience 2022Quote: ... the c-organoids were cryoprotected in 30% sucrose overnight at 4°C followed by flash freezing in OCT Compound (ThermoFisher) and stored at −20°C ...
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bioRxiv - Microbiology 2023Quote: ... The epidermis was peeled off and discarded and the dermis was minced into small pieces and digested at 37°C for 1 h following 4°C for overnight using collagenase IV (17104019, Gibco). The resulting cell suspension was filtered through a 70 μm cell strainer (BD Falcon) ...
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Direct analysis of ribosome targeting illuminates thousand-fold regulation of translation initiationbioRxiv - Molecular Biology 2020Quote: ... and 3′ biotinylated using the Pierce RNA 3′end biotinylation kit (Thermo Scientific 20160).
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bioRxiv - Molecular Biology 2021Quote: ... counted and reseeded in 3 mL A medium (1:3 mix DMEM/F12 (Gibco) and Neurobasal (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... 3 μl were sampled on a 3 well Diagnostika slides (X1XER303B) from Thermo scientific for observation on an Zeiss LSM710 confocal microscope equipped with a Plan-Apochromat 63×/1.4 Oil objective and 405 nm and 488 nm lasers ...
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bioRxiv - Microbiology 2023Quote: ... 3′RNA-seq libraries were analyzed on a Qubit 3 Fluorometer (Thermo Fisher Scientific) and an Agilent 4200 TapeStation System prior to paired- end sequencing using the HiSeq 2500 system (Illumina).
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bioRxiv - Neuroscience 2022Quote: ... 3 μg DNA and 3 μL Lipofectamine in 300 μl Optimem (Thermofisher Scientific, USA) were used per well containing 700 μl DMEM ...
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bioRxiv - Bioengineering 2023Quote: ... and EDC (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... 3 mg of solid red DiI (1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate, Molecular Probes) dissolved in methylene chloride were mixed with 50 mg of tungsten beads (1.3 microns in diameter ...
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bioRxiv - Neuroscience 2020Quote: ... a selected cell terminal was puffed for 5 s with a solution containing 3-5 μM FM1-43 (Molecular Probes) and (in mM) ...
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bioRxiv - Microbiology 2020Quote: The Q577R gp41 change was introduced into pSHIV-AD8-EO via site-directed mutagenesis using 5’p-TCAAGCAGCTCCGGGCAAGAGTCC-3’ (forward) and 5’p-TGCCCCAGACTGTGAGTTGCAACA (reverse) with Platinum SuperFi PCR mastermix (ThermoFisher) as described in the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then washed 3 x 5 min in PBS and incubated for 5 min in DAPI (Invitrogen, cat# D1306) 1:1000 in PBS ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 1.5 µg of total RNA of each sex were subjected to 5’ and 3’ RACE with a GeneRacer kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... followed by 40 cycles at 95°C for 15 sec then 60°C for 1 min using a QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific). Extracted MAC DNA was used as the quantification standard.
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bioRxiv - Microbiology 2020Quote: ... 5-(and-6)-carboxyfluorescein (FAM) and 5-(and-6)-carboxytetramethylrhodamine (TAMRA) were obtained from Invitrogen™ ...
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bioRxiv - Bioengineering 2022Quote: ... 4 μl of 4 mM resazurin sodium salt (Acros Organics, Belgium), 0.002 U purified Castellaniella defragrans geraniol dehydrogenase ...
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bioRxiv - Neuroscience 2021Quote: ... at 6-7 days in vitro (DIV) or Lipofectamine 2000 (Thermofisher) 24/72 hours before the experiments ...
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bioRxiv - Cell Biology 2021Quote: ... iPSCs were passaged every 6-7 days with Versene (Life Technologies) at a 1:12 ratio ...
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bioRxiv - Cell Biology 2022Quote: ... DCFH-DA (6-carboxy-2′,7′- dichlorodihydrofluorescein diacetate) was from Invitrogen. DMSO and Evans Blue Dye were purchased from Sigma Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... for 30 min followed by glycerol in PBS (volume ratio 7:3) for 30 min before being mounted onto superfrost slides (Fisher Scientific) for imaging ...
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bioRxiv - Neuroscience 2021Quote: ... CS03iCTRn2 hPSCs were dissociated with Versene and colonies were transferred to an ultra-low attachment T-25 flask containing EZ sphere culture medium (a mixture of DMEM and F-12 medium in a 7:3 ratio supplemented with 1× B-27 supplement minus vitamin A [Life Technologies] ...
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bioRxiv - Neuroscience 2022Quote: ... the percentage of apoptotic astrocytes was evaluated using CellEvent Caspase-3/7 Green Detection Reagent (1:250; Thermo Fisher, cat. #C10423) added directly to the medium ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then resuspended in DMEM + 10% FBS supplemented with 50 μM CellEvent™ Caspase 3/7 Green Detection Reagent (Invitrogen) at 37 °C for 25 min ...
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bioRxiv - Genomics 2020Quote: ... 4 μM glass slide) then for Laser Capture Microdissection (LCM; N=3; 7 μM glass slide coated with polyethylene naphthalate – ThermoFisher #LCM0522). The slides were stored at −20°C in an airtight container with desiccant until ready for dissection (1 day to 3 months) ...
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bioRxiv - Systems Biology 2021Quote: ... coli phosphate-binding protein labeled with 7-Diethylamino-3-[N-(2-maleimidoethyl)carbamoyl]coumarin (MDCC) (phosphate sensor, CAT # PV4406, Thermo Fisher) upon binding of the free phosphate GTP hydrolysis product (excitation at 425 nm ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were incubated for 30 minutes with 40 µl/ml CellEvent™ Caspase-3/7 Green ReadyProbes Reagent (#R37111, Life Technologies). Cells were washed with PBS (without Mg2+ and Ca2+) ...
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bioRxiv - Cancer Biology 2022Quote: ... At time of treatment the cells were also treated with 2μM CellEvent caspase-3/7 green detection reagent (C10423; Invitrogen, ThermoFisher Scientific, Inc), which was used to measure apoptosis ...
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bioRxiv - Neuroscience 2022Quote: To analyze effector caspase activity in stimulated hippocampal neurons we employed the CellEvent(tm) caspase-3/7 green detection assay (ThermoFisher Scientific) that allows analyzing activity in individual cells ...
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bioRxiv - Neuroscience 2022Quote: ... and medium from day 3-7 is Neurobasal Plus medium (NB-Plus) supplemented with B-27 μg/mL (Thermo Fisher Scientific), GlutaMax 10 μg/mL (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: Crude protein extraction obtained as described above was electrophoresed through a slab isoelectric focusing gel (pH 3-7, Invitrogen Novex EC66452) employing freshly made cathode and anode buffers (Novex) ...
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bioRxiv - Cell Biology 2022Quote: ... Single-axis tilts were collected from −60° to +60° at 2° increments at 7 □µm defocus on a Falcon 3 camera (Thermo Fisher) operated in linear mode at 22,000X magnification ...
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bioRxiv - Biophysics 2023Quote: ... the media was removed and the cells were incubated with 8 µM CellEventTM Caspase 3-7 green detection reagent (Thermo Fisher) in PBS containing 5% FBS for 30 min at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... as well as between stratified patient plasma samples (n=7) over three time points using an IL-23 Human ELISA Kit (BMS2023-3, ThermoFisher, Canada), as per manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Serum starved PANC-1 and MiaPaCa-2 cells were incubated with human properdin (20 µg/ml) and CellEvent™ Caspase-3/7 Green Detection Reagent (Invitrogen) (5 μM ...
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bioRxiv - Cell Biology 2023Quote: ... BAL samples were then centrifuged at 3,000 g for 20 min to collect cells and ApoBDs for staining with a combination of SR-DEVD-FMK (caspase 3/7 activity detection dye, Thermo Fisher), annexin A5-V450 and TO-PRO-3 in 1× A5 binding buffer at room temperature for 10 min ...
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bioRxiv - Immunology 2024Quote: ... and normal tissues were mechanically cut into 3-7 mm fragments and incubated in RPMI 1640 medium (Gibco™, cat. 42401042) containing 1 mg/ml Liberase TL Research Grade (Roche ...
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bioRxiv - Immunology 2024Quote: ... Cells were pelleted onto glass slides via cytospin centrifugation at 700 rpm for 7 min and stained with Hema 3 (Fisher Scientific). Neutrophils ...
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bioRxiv - Immunology 2024Quote: ... The cell suspension was slowly layered on 70% Percoll solution [for 10 ml: 7 ml 100% Percoll solution with 3 ml 1x HBSS (14175-053, Gibco™)] in a 15 ml Falcon tube and centrifuged for 30 min (500 g ...
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bioRxiv - Immunology 2024Quote: ... 6-well culture plates) for 8 days at 37°C and 5% CO2 in RPMI1640 (Life technologies) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2020Quote: ... One portion was incubated for 16 h at 4°C with Dynabeads (Thermo Fisher Scientific) directly conjugated to anti-CD9 antibody with rotation ...
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bioRxiv - Microbiology 2021Quote: ... overnight at 4 °C followed by Goat-anti Mouse IgG-HRP secondary antibody (Invitrogen™) at room temperature for 2 hours ...
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bioRxiv - Cell Biology 2022Quote: ... overnight at 4 °C and then incubated with fluorescence-conjugated secondary antibody (Thermo Fisher Scientific) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM ThDP) at 4 °C using a 20 kDa MWCO dialysis cassette (Thermo Scientific).