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Citations for Thermo Fisher :
2201 - 2250 of 5822 citations for ZBED1 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... along with goat anti-human IgG Fc secondary antibody (polyclonal, ThermoFisher). HLA-ESC was detected with an anti-HLA-E antibody (clone 3D12 ...
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bioRxiv - Molecular Biology 2023Quote: ... and activated with Dynabeads Human T Activator CD3/CD28 (Gibco, USA) at a 1:1 ratio for 24 hours ...
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bioRxiv - Immunology 2023Quote: Adipocytes were differentiated from human adipose-derived stem cells (ADSCs, ThermoFisher), which were obtained from human lipoaspirate tissue and cryopreserved from primary culture ...
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bioRxiv - Immunology 2023Quote: ... were obtained from ThermoFisher and maintained in Human Large Vessel Endothelial Cell Basal Medium (ThermoFisher) according to manufacturer’s protocol.
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Chimeric Antigen Cytotoxic Receptors for In-Vivo Engineering of Tumor-targeting Natural Killer CellsbioRxiv - Immunology 2023Quote: ... Cytokine & Chemokine Convenience 34-Plex Human ProcartaPlex™ Panel 1A (Invitrogen) was used to collect and analyze the supernatant for levels of cytokines/chemokines following the protocol provided by the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... CCL5 and CCL2 (Human cytokine magnetic 25-plex panel; Life technologies).
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bioRxiv - Immunology 2023Quote: ... and erythrocytes using the Dynabeads Untouched Human Monocytes Kit (Invitrogen, 11350D) (Supplemental Figure 2).
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bioRxiv - Developmental Biology 2023Quote: ... KiPS line was derived by reprogramming of commercial human keratinocytes (Invitrogen) with Sendai viruses encoding for OSKM 62 and kindly provided by Austin Smith’s laboratory ...
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bioRxiv - Cell Biology 2023Quote: Human embryonic kidney HEK293T cells were maintained in DMEM/F12 (Gibco) with 10 % FCS (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... miBrains were cultured in Human Endothelial Serum-Free Medium (ThermoFisher Scientific) with 1% pen/strep (Sigma-Aldrich) ...
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bioRxiv - Genomics 2023Quote: Commercially available human iPSC lines derived from a female donor (Gibco Episomal hiPSC ...
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bioRxiv - Immunology 2024Quote: ... Human embryonic kidney (HEK) epithelial 293FT cells were ordered from Invitrogen. A549 cell lines were maintained in F-12K media with 10% fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2024Quote: ... rabbit anti-human CD16 (clone: JE49-79) polyclonal antibody (Invitrogen, USA) and mouse anti-human CD64 (clone ...
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bioRxiv - Immunology 2024Quote: ... Sorted T cells were stimulated with Human T-Activator Dynabeads (Thermofisher) in RPMI-1640 (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... using the Dynabeads Untouched Human CD4 T cell kits (Thermofisher, USA). T lymphoblast were generated by culturing T cells at 2 million/mL onto P12 wells on RPMI 1640 with FBS (10 %) ...
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bioRxiv - Microbiology 2024Quote: ... followed by AlexaFluor 647-conjugated anti-human antibodies (Invitrogen, 1:1,000). Slides were then stained with 1 μg/mL DAPI and mounted in Citifluor (Citifluor Ltd. ...
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bioRxiv - Immunology 2024Quote: ... and biotinylated human BCMA (Bio-connect) with streptavidin-PE (Thermo Fisher). For intracellular staining ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 20 ng/ml human epidermal growth factor (Invitrogen, PHG0311), 20 ng/ml human basic fibroblast growth factor (Stemcell Technologies ...
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bioRxiv - Genetics 2024Quote: ... supplemented with human keratinocyte growth supplement (HKGS, Gibco™, cat. #S0015), containing Human growth factor I insulin-like recombinant ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... Ion AmpliSeq transcriptome human gene expression panel (Thermofisher Scientific, Cat#A31446) was added to the Reagents cartridge ...
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bioRxiv - Microbiology 2024Quote: ... were cultured in human B+ erythrocytes in RPMI 1640 medium (GIBCO) supplemented with 10% human type AB-positive plasma ...
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bioRxiv - Molecular Biology 2023Quote: Full length cDNAs encoding human TOPORS WT (codon optimized, Invitrogen GeneArt), *RING (I105A ...
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bioRxiv - Molecular Biology 2024Quote: ... total RNAs were processed with RiboMinus kit (Human/ Mouse Module, Invitrogen) to deplete most ribosomal RNAs (ribo-RNA) ...
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bioRxiv - Immunology 2024Quote: ... fibrinogen from human plasma AF488 Conjugate (Thermo Fisher Scientific, Cat# F13191), platelet-poor plasma ...
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bioRxiv - Immunology 2024Quote: ... and ELISA analysis (human IL-1β ELISA Kit, Thermo Fisher Scientific) according to the manufacturer ‘s instructions.
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bioRxiv - Neuroscience 2024Quote: ... FITC anti-human/mouse CD45R (1:500, #11-0452-82, Invitrogen), PE anti-mouse CD3ε (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... Treatment with recombinant 10 U/mL human IFNγ (ThermoFisher Scientific RIFNG100) was done at 24 hours post-infection ...
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bioRxiv - Neuroscience 2024Quote: Human embryonic kidney (HEK) 293T cells were cultured in DMEM (GIBCO), 10% FBS ...
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bioRxiv - Microbiology 2024Quote: ... and donkey anti-human IgG-Daylight 550 (Thermo Fisher, SA5-10127). The stained parasites were acquired by Attune NxT flow cytometry ...
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bioRxiv - Bioengineering 2024Quote: ... 5250 ng/mL human recombinant IL-6 protein (Invitrogen; Waltham, MA) was added to the experimental group and an equal volume of 1X PBS was added to the control group ...
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bioRxiv - Synthetic Biology 2023Quote: ... and F-FGF21ΔNΔC) to human FZD7 CRD and βKlotho (Fisher Scientific) or L-39F7 series (L-39F7 ...
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bioRxiv - Cell Biology 2024Quote: ... SASP factors: human IL-8 Uncoated ELISA (88-8086-88, Invitrogen), human IL-6 Uncoated ELISA (88-7066-88 ...
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bioRxiv - Cell Biology 2024Quote: ... and human GRO alpha (CXCL1) Uncoated ELISA (88-52122-22, Invitrogen). Collagen type II synthesis ...
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bioRxiv - Cancer Biology 2024Quote: ... 50 µl Human Epidermal Growth Factor (hEGF; 500 µg/ml, Invitrogen), 50 µl human Fibroblast Growth Factor-10 (hFGF-10 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Human GPR1 was cloned into a pFastBac vector (Invitrogen, Carlsbad, CA) for protein expression and purification ...
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bioRxiv - Plant Biology 2019Quote: ... Aliquots were analyzed on a 10% SDS-PAGE followed by Coomassie Blue staining or Western blot analysis with goat α-6X-His-HRP antibody (Life Technologies, Carlsbad, CA) at a 1:5,000 dilution in Tris-buffered saline (20 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The gene optimized for the Drosophila codon usage and extended by C-terminal His-tag was purchased from Life Technologies (DNA String fragments, USA). The DNA fragment was inserted into a pMT-BiP-V5-His_A vector (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: 1-2 μg of purified 6xHis-tagged calcineurin or 6xHis-MBP as a negative control was first bound to 10 μL magnetic Dynabeads™ His-Tag Isolation and Pulldown Beads (Thermo Fisher Sci., 10104D) in 490 μL base buffer (50 mM Tris-HCl pH 7.5,150 mM NaCl ...
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bioRxiv - Plant Biology 2019Quote: ... 1 μL of each reaction was added to a mix containing 9 μL of Hi-Di Formamide (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) and 0.5 μL of GeneScan 500 LIZ Size Standard (Applied Biosystems ...
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bioRxiv - Plant Biology 2019Quote: ... 1 μL of each reaction was added to a mix containing 9 μL of Hi-Di Formamide (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) and 0.5 μL of GeneScan 500 LIZ Size Standard (Applied Biosystems ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by sequencing using the Ion PI™ Hi-Q™ Sequencing 200 Kit on Ion Proton PI™ V2 chips (Thermo Fisher Scientific), an Ion Proton™ System ...
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bioRxiv - Genetics 2020Quote: ... in all the above clonings except for the his-72::GFP repair template which required ccdB resistant bacteria for which we used One Shot ccdB Survival (Thermo Fisher Scientific, cat. A10460). DNA extractions by miniprep were done with the ZymoPURE Plasmid Miniprep kit and elution with water (Zymo Research ...
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bioRxiv - Immunology 2020Quote: ... Nunc MaxiSorp ELISA plates were coated with 50 μL of capture antibody (anti-6x-His antibody clone HIS.H8; 2 μg/mL; Thermo Fisher Scientific, Waltham, MA, USA) in phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2023Quote: ... Protein bands were visualized with Coomassie staining (ROTIBlue Quick, Carl Roth) or immunodetected with a monoclonal anti-His antibody conjugated to HRP (MA1-21315-HRP, Thermo Fisher, 1:2000 diluted). A detailed protocol can be found in protocols.io (dx.doi.org/10.17504/protocols.io.bu67nzhn).
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bioRxiv - Genomics 2023Quote: ... Vero/hSLAM and VeroE6/TMPRSS2 were maintained in supplemented DMEM with 10% HI-FBS and 1 mg/mL G418 sulfate (Thermo Fisher 10-131-027). HeLa/ACE2/TMPRSS2 cells were a generous gift from Dr ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% (v/v) heat-inactivated (HI) FBS (Thermo Fisher Scientific, Waltham, MA, USA). Stable cell lines expressing F-CIC-S ...
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bioRxiv - Pathology 2023Quote: ... The cDNA was used for RT-qPCR with qPCRBIO SyGreen Blue mix Hi-ROX (PCR Biosystems, London, UK) on the StepOne Plus Real-Time PCR machine (Applied Biosystems, Foster City, CA) and primers listed in Table S2 ...
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bioRxiv - Microbiology 2024Quote: ... BMDMs were plated a day in advance on a coverslip lined 24-well plate at 8 x105 cells in 1mL per well, in “BMDM media” supplemented with 10% L929 (20% HI FBS, 1% penicillin-streptomycin, 1% non-essential amino acids (ThermoFisher, cat#11-140-050), 1% sodium pyruvate) ...
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bioRxiv - Microbiology 2024Quote: ... The amplified PCR product of the C-His oipA gene fragment was cloned into vector pLATE11 using aLICator LIC Cloning and Expression Kit 1 (Thermo Fisher Scientific, United States) by following the kit’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cDNA plasmids in pcDNA™4/TO Mammalian Expression Vector were transiently transfected into Expi293F™ cells stably expressing human FHF2b and human SCN1B subunit (polyclonal) background using ExpiFectamine™ 293 Transfection Kits (Gibco,Thermo Fisher Scientific CAT #: A14524). Induction was achieved using Tetracycline (Sigma Aldrich) ...