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Citations for Thermo Fisher :
2201 - 2250 of 5129 citations for SPFH1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... BV-2 cells were fixed and sequentially incubated with Mouse-on-Mouse IgG Blocking Solution (Invitrogen R37621) and Navenci blocking solution at 37°C for 60 min each in a pre-heated humidified chamber ...
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bioRxiv - Immunology 2024Quote: ... The fixed slides were washed with PBS for twice and blocked with Blocking buffer (Thermo Fisher Scientific) for 1 h at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... for 1 hour before blocking with 3% (w/v) bovine serum albumin (BSA, Fisher Scientific, Cat # BP9703100). To detect ARP3 and GLUT4 ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were blocked with 1X BlockerTM FL 10X Fluorescent blocking buffer (Thermo Fisher Scientific, Cat # 37565) followed by incubation with the respective primary antibodies overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... 1% OVA in PBST for the second round and ELISA Blocker Blocking Buffer (Thermo Fisher Scientific, N502) for the third round ...
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bioRxiv - Developmental Biology 2023Quote: ... oocytes were incubated with the secondary antibodies (1:500 in blocking solution, Alexa Fluor, Thermo Fisher Scientific) for 1 hour in dark ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were diluted in 7.5ml Intercept PBS blocking buffer with 0.1% Tween (SynGAP1; 1:1000dil, Invitrogen, PA1-046 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections stained with mouse primary antibodies were blocked in ReadyProbes “Mouse on Mouse” IgG blocking solution (Thermofisher) for 1 hour before the initial blocking step ...
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bioRxiv - Immunology 2023Quote: ... was used at 1:8000 dilution in blocking solution together with 0.01 mg/ml DAPI (Thermo Fisher). The wells were washed with wash buffer and PBS ...
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bioRxiv - Microbiology 2023Quote: ... followed by destaining using 25 mM NaOH and blocking using 1x BlockerTM FL Fluorescent protein block (ThermoFisher). Primary antibodies used were Anti-GapDH (G9545 ...
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bioRxiv - Genetics 2023Quote: ... 0.08% blocking reagent) containing 10 µL H2O2 0.3% and 2.5 µL of Alexa488 fluorochrome-labeled tyramides (Invitrogen). Sections were washed two times in PBS for 10 min at RT ...
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Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat productionbioRxiv - Bioengineering 2023Quote: ... and blocked for 45 minutes using a blocking buffer of DPBS with 5% goat serum (ThermoFisher #16210064) and 0.05% sodium azide (Sigma #S2002) ...
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bioRxiv - Neuroscience 2023Quote: ... incubated with Alexa 647 labeled donkey anti-rabbit secondary antibodies (1:250 in blocking solution; Invitrogen A32795TR) for 1 h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... A blocking solution was then added with 0.1% Triton-X-100 and 3% bovine serum albumin (Invitrogen). An antibody solution of MAP2 (1:2500 ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was blocked for 1 h at RT with nucleic acid detection blocking buffer (Life Technologies), then probed for 1 h at RT with streptavidin-HRP 1:5,000 (Genescript ...
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bioRxiv - Developmental Biology 2023Quote: ... and incubated in blocking solution with goat or donkey raised Alexa Fluor-conjugated secondary antibodies (Thermo Fisher), at room temperature for 24 hours ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were incubated in a blocking solution containing 5% goat serum (16210–064, Thermo Fisher Scientific), 3% bovine serum albumin (BAC62 ...
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bioRxiv - Cancer Biology 2024Quote: ... blocking with 1% BSA and staining with rhodamine-conjugated phalloidin following manufacturer’s instructions (Invitrogen, Waltham, MA, USA) and Hoechst (15 min ...
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bioRxiv - Bioengineering 2024Quote: ... and acetylated tubulin staining: 2 mL blocking buffer containing 20 μL anti-Sox2 (Invitrogen, 14-9811-82), 20 μL anti-Myt1 (Abcam ...
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bioRxiv - Microbiology 2024Quote: ... the protocol remained the same except as follows: Blocking was done in TBST + 7.5% goat serum (GIBCO). The primary antibody incubation step included both mouse anti-N-extein and rabbit anti-C-extein antibodies (polyclonal sera ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were blocked in blocking buffer composed of 10% normal goat serum (NGS; Gibco, cat# 16210-064) and 0.3% Triton X-100 (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... The suspension for blocking and antibody incubations was 1x Tris buffered saline (Thermo Fisher Scientific, J60877.K3), with 0.1% Tween 20 (Merck ...
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bioRxiv - Microbiology 2024Quote: ... the wells were blocked with 200 μl of blocking buffer containing 1x PBS (ThermoFisher Scientific, MA, USA), 0.05% of Tween 20™ (ThermoFisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... and secondary antibodies in blocking buffer were added (1:200) [donkey Anti-Goat (Invitrogen; A11057 or A32814), Donkey Anti-Mouse (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... Peptides were loaded onto the trapping column (PepMap100, C18, 300 µm x 5 mm, Thermo Scientific) using partial loop injection with 2% acetonitrile (ACN) ...
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bioRxiv - Cancer Biology 2021Quote: ... fractionated into 8 fractions using the High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific 84868) according to the manufacturer protocol and dried ...
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bioRxiv - Cancer Biology 2021Quote: Peptide mixtures were separated by reversed-phase chromatography on an EASY-Spray column (Thermo Fisher Scientic), 25-cm long (inner diameter 75 μm ...
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bioRxiv - Biochemistry 2021Quote: ... with 15 µM Impβ mixed with 75 µM Rev-peptide and 1x SYPRO Orange (Molecular Probes) diluted in H2O ...
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bioRxiv - Cell Biology 2020Quote: ... Cleaved peptides were analyzed by nanoflow LC-ESI-MS/MS (Thermo Scientific LTQ Orbitrap Velos Pro). Peptide sequences were mapped using ProteinProspector and Mascot software packages.
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bioRxiv - Cell Biology 2020Quote: ... The isobaric labelling of the peptides was performed using 10-plex TMT reagents (Thermo Fisher Scientific).
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REEP4 is recruited to the inner nuclear membrane by ELYS and promotes nuclear pore complex formationbioRxiv - Cell Biology 2020Quote: Peptides were analyzed by C18 nanoflow reversed-phase HPLC (Dionex Ultimate 3000 RSLC nano, Thermo Scientific) combined with orbitrap mass spectrometer (Q Exactive HF Orbitrap ...
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bioRxiv - Microbiology 2021Quote: ... Peptides were loaded with the autosampler directly onto a 50 cm EASY-Spray C18 column (ThermoFisher). Peptides were eluted from the column using a Dionex Ultimate 3000 Nano LC system with a 14.8 min gradient from 1% Buffer B to 23 % Buffer B (100% acetonitrile ...
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bioRxiv - Developmental Biology 2020Quote: ... desalting and fractionation was performed with Pierce High pH Reversed-Phase Peptide Fractionation Kits (Thermo Scientific) to generate 8 fractions per sample ...
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bioRxiv - Developmental Biology 2022Quote: ... Peptide desalting was performed according to the manufacturer’s instructions (Pierce C18 Tips, Thermo Scientific, Waltham, MA). Desalted peptides were reconstituted in 0.1% formic acid in water and further separated into four fractions by strong cation exchange chromatography (SCX ...
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bioRxiv - Molecular Biology 2020Quote: ... Peptide desalting was performed according to the manufacturer’s instructions (Pierce C18 Tips, Thermo Scientific, Waltham, MA). Eluates were lyophilized and reconstituted in 11 µL of 5% acetonitrile and 2% formic acid in water ...
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bioRxiv - Microbiology 2021Quote: Peptides were labelled with Alexa Fluor® 488 (A488) 5-sulfodichlorophenol ester (SDP) (Thermo Fisher Scientific) using previously described methods [66] ...
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bioRxiv - Immunology 2021Quote: ... The digested peptides were labelled with iTRAQ reagents according to the manufacturer’s protocols (Applied Biosystems, USA). The peptides from the Hemocyte samples collected at four different time points were labelled with four 8-plex iTRAQ reagents (Applied Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... Peptides were analyzed on a hybrid quadrupole orbitrap mass spectrometer (Orbitrap Exploris 480; Thermo Fisher Scientific) equipped with an Easy1200 nanoLC system (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... Peptides were eluted from a 75 µm x 50 cm analytical C18 column (PepMan, Thermo Scientific) on a linear gradient running from 4% to 64% acetonitrile over 135 min ...
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bioRxiv - Microbiology 2019Quote: ... 0.8-3 μg peptide were loaded with an UltiMateTM 3000 RSLCnano Liquid Chromatograph (Thermo Fisher Scientific) in loading solvent A (2% acetonitrile ...
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bioRxiv - Neuroscience 2019Quote: ... and treated with 10 μM of freshly prepared amyloid-β peptide 1-42 (Aβ42, Life technologies) for 24 h ...
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bioRxiv - Molecular Biology 2019Quote: ... the peptides were trapped on an Acclaim PepMap C-18 precolumn (0.3×5mm, Thermo Fisher Scientific), using a flowrate of 25 µl/min and 100% buffer A (99.9% H2O ...
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bioRxiv - Biochemistry 2019Quote: ... The peptides were analyzed by an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific, USA)[32] ...
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bioRxiv - Cell Biology 2019Quote: ... Peptides (100ng) were separated on a Thermo ScientificTM EASY-nLC 1000 HPLC system (Thermo Fisher ScientificTM) for 1hour from 5-60% acetonitrile with 0.1% fromic acid and directly sprayed via a nanoelectrospray source in a quadrupole Orbitrap mass spectrometer (Q ExactiveTM ...
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bioRxiv - Microbiology 2019Quote: ... Peptides were resolved on the analytical column (PepMap C18, 75 μm×50 cm, 2μ, Thermo Scientific) at 300 nL/min flow rate using a step gradient of 5% to 22% solvent B (0.1% formic acid in 95% acetonitrile ...
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bioRxiv - Systems Biology 2019Quote: ... Peptides were analyzed using a Q Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher Scientific), equipped with a Nanospray Flex Ion Source (Thermo Fischer Scientific) ...
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bioRxiv - Biochemistry 2019Quote: ... peptides were ionized by nanoelectrospray and analyzed using a QExactive HF-X mass spectrometer (ThermoFisher Scientific). The full MS-scan was followed by MS/MS for the top 15 precursor ions in each cycle ...
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bioRxiv - Neuroscience 2019Quote: ... or 10 μM of freshly prepared human synthetic amyloid-β peptide 1-42 (Aβ42, Life technologies) for 24 h as described (Kiyota et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... Larval peptides were analyzed on an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific, Waltham, MA, USA) with a 4.5 cm ...
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bioRxiv - Synthetic Biology 2019Quote: ... peptide separation was carried out using an EASY nLC-1000 system (Thermo Fisher Scientific, MA, USA) equipped with a RP-HPLC column (75 μm × 30 cm ...