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Citations for Thermo Fisher :
2201 - 2250 of 10000+ citations for Recombinant Goat VEGFA Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Protein concentrations were determined by a BCA Protein assay (Thermo Fisher).
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration was assessed using BCA protein assay (Thermo Fisher Scientific). Protein electrophoresis was performed using 4–15% Mini-PROTEAN TGX Precast acrylamide gel (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 μl Dynabeads Protein A and Protein G (Thermo Fisher Scientific) were pre-incubated overnight with rotation in the presence of 5 mg/ml BSA and 10 μg antibody (MYCN (B8.4.B) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration was determined with the Qubit protein assay (ThermoFisher Scientific) following manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were quantified using BCA Protein Assay Kit (Thermo Scientific) following the manufacturer’s protocol ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Protein concentration was measured using BCA Protein Assay (Fisher Scientific, PI23227). 20 µg of protein was removed for total protein measurements ...
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bioRxiv - Neuroscience 2024Quote: ... Protein estimation was carried out using BCA protein estimation kit (Invitrogen).
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was measured by BCA protein assay (Thermo Fisher Scientific). Fifteen to thirty micrograms of lysates were separated on 4-15% precast Mini-PROTEAN® TGX™ gels (Bio Rad ...
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bioRxiv - Plant Biology 2024Quote: ... Protein concentration then determined using a Qubit protein assay kit (Invitrogen). The MUG fluorescent assay was performed in duplicates with 20 µl protein extract in MUG assay buffer (50mM phosphate buffer (pH 7.0) ...
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bioRxiv - Cancer Biology 2021Quote: ... The secondary antibodies used for detection were horseradish peroxidase (HRP)–conjugated goat anti-mouse and goat anti-rabbit IgG (1:5,000; Thermo Scientific). The blots were incubated with Luminata Western HRP substrate (Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... Immuno-complexes were visualized with Alexa Fluor 488-labeled goat anti-rabbit IgG antibody and Alexa Fluor 568-labeled goat anti-guinea pig IgG antibody (Invitrogen). Cells were observed by a confocal laser microscope (LSM710 with Axio Observer Z1 ...
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bioRxiv - Cell Biology 2020Quote: ... The secondary antibodies used were goat anti-rabbit and goat anti-mouse conjugated to Alexa Fluor 488 or 568 (1:1000, Invitrogen). To distinguish individual cells for quantification of MUC5AC-positive cells ...
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bioRxiv - Cell Biology 2020Quote: Secondary antibodies were as follows: Alexa Fluor 488- or Alexa Fluor 594-conjugated to goat anti-mouse or goat anti-rabbit IgG (Invitrogen), Alexa Fluor 568-conjugated to goat anti-rat IgG (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated for 2 hrs with Alexa Fluor 555-conjugated goat anti-rat antibody and Alexa Fluor 488-conjugated goat anti-mouse antibody (both 1:200; Invitrogen). Fiber images were acquired by fluorescence microscopy and analyzed manually using Image J ...
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bioRxiv - Genetics 2021Quote: ... Alexa Fluor 488 Goat Anti-mouse IgG (1:300) and Alexa Fluor 568 Goat Anti-rabbit IgG (1:300) from Invitrogen. Digital images were obtained using Leica TCS SP8 LIGHTNING confocal microscope equipped with motorized stage and LAS X software ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated for 2h at room temperature with a goat anti-rat antibody and/or a goat anti-rabbit antibody or Alexa-647 phalloidin (Invitrogen) if needed ...
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bioRxiv - Immunology 2020Quote: ... and the secondary antibodies including horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (#31439) and HRP-conjugated goat anti-human IgG (#31410) were purchased from Invitrogen. The secondary antibody for HRP-conjugated goat Anti-rabbit IgG (#12-348 ...
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bioRxiv - Developmental Biology 2020Quote: ... and secondary antibodies (Goat α-Rabbit Alexa Fluor 633; Goat α-Mouse Alexa Fluor 488 and DAPI, Thermo Fisher Scientific) were incubated overnight in blocking solution at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... immunostaining was completed by a 1 hour room temperature incubation with secondary antibodies (goat anti-rabbit Alexa Fluor 488, goat anti-mouse Alexa Fluor 546, and goat anti-chicken Alexa Fluor 647; 1:1,000 dilution; ThermoFisher Scientific). After additional washes ...
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bioRxiv - Genomics 2020Quote: ... Goat anti-Rabbit IgG (H+L)-HRP (Bio Rad, 1706515) and Goat anti-Mouse IgG (H+L)-HRP (Invitrogen, 31430) were used as secondary antibody for western blotting.
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bioRxiv - Cell Biology 2020Quote: ... The membranes were washed three times in PBS and incubated with Goat Anti-Rabbit HRP or Goat Anti-Mouse HRP secondary antibody (1:10,000; ThermoFisher Scientific) in PBST either at room temperature for 2 h or overnight at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... Coverslips were then incubated with goat anti-rabbit or goat anti-mouse secondary antibodies conjugated to Alexa-fluorophores (1:1000; Invitrogen) for 1 h at RT.
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bioRxiv - Biochemistry 2019Quote: ... MilliporeSigma) and a mixture of goat anti-rabbit and goat anti-mouse DyLight secondary antibodies (both from Thermo Fisher Scientific). Blots were scanned with an Odyssey CLx (LI-COR Biosciences) ...
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bioRxiv - Neuroscience 2019Quote: ... Alexa488- and Cy5-conjugated goat α-guinea pig IgG and goat α-rabbit IgG secondary antibodies (Molecular Probes; Jackson Immunoresearch) were used at 1:500 ...
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bioRxiv - Developmental Biology 2019Quote: ... sections were incubated during 90 minutes at room temperature with secondary antibodies (Alexa Fluor 488 goat anti-rabbit, Alexa Fluor 555 goat anti-chicken; Invitrogen) diluted 1:1,500 in blocking solution ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by incubation with goat anti-rabbit IgG Alexa Fluor 488 and goat anti-mouse IgG Alexa Fluor 568 secondary antibodies (Invitrogen). Coverslips were mounted in Mowiol 4-88 (Calbiochem ...
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bioRxiv - Biophysics 2020Quote: ... AF647-conjugated goat anti-mouse-IgG (catalogue #A21236) and AF647-conjugated goat anti-rabbit-IgG (catalogue #A21244) were purchased from ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... the membranes were incubated with the recommended dilution of HRP-conjugated secondary antibody (goat anti-rabbit IgG HRP 1:10000, Invitrogen; goat anti-mouse IgG HRP 1:10000, Invitrogen) at room temperature for 1 h ...
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bioRxiv - Neuroscience 2020Quote: ... slides were incubated with fluorophore-conjugated secondary antibodies (Alexa Fluor goat-anti rabbit 488 nm, goat anti-rat 594 nm, Invitrogen) for 1 h at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... Wells were consecutively washed two times for 5 min in washing buffer before incubation with secondary antibodies (Alexa Fluor-488 goat anti-mouse IgG, Invitrogen, A-11029; Alexa Fluor-647 goat anti-mouse IgG, Invitrogen, A-21236 ...
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bioRxiv - Cell Biology 2021Quote: ... Alexa-Fluor-488-conjugated goat anti-mouse-IgG antibodies and Alexa-Fluor-594-conjugated goat anti-mouse-IgG antibodies (Thermofisher) were used.
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bioRxiv - Neuroscience 2019Quote: ... and subsequently incubated in secondary antibody (goat anti-chicken 488 1:200, Invitrogen A32931; goat anti-rabbit 647 1:200, Invitrogen A21244 ...
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bioRxiv - Neuroscience 2019Quote: ... and subsequently incubated in secondary antibody (goat anti-chicken 488 1:200, Invitrogen A32931; goat anti-rabbit 647 1:200, Invitrogen A21244; goat anti-rabbit 568 1:200, Invitrogen A11011 ...
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bioRxiv - Microbiology 2021Quote: ... slides were treated with appropriate secondary antibodies (1:1000 goat anti-rat/rabbit Invitrogen, 1:1000 goat anti-chicken, Abcam) following PBS rinsing ...
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bioRxiv - Microbiology 2021Quote: ... and incubated for three hours at 37°C in 2% BSA in PBS complemented with secondary antibody (goat anti-rabbit-Oregon Green, goat anti-mouse-A594, Invitrogen). Gels were washed three times in PBST before a second overnight expansion in ddH2O ...
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bioRxiv - Microbiology 2021Quote: ... the goat anti-ACE2 primary antibody (R&D) and Alexa Fluor 568 donkey anti-goat IgG (H+L) secondary antibodies (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed for 60 min with TBST at room temperature and then incubated for 2 h at room temperature with goat anti-rabbit (H+L) or goat anti-mouse HRP-linked secondary antibodies (Invitrogen) respectively ...
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bioRxiv - Cell Biology 2021Quote: ... and detected using horseradish peroxidase-conjugated goat-a-mouse or goat-a-rabbit secondary antibodies followed by chemiluminescence (Thermo Scientific). Chemiluminescent membranes were exposed to X-ray film for 5–180 s and developed with a Konica SRX-101A film processor ...
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bioRxiv - Cell Biology 2021Quote: ... incubated with goat anti-mouse or goat anti-rabbit secondary antibody conjugated to horseradish peroxidase (Thermo Fisher Scientific, 1:5,000), washed ...
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bioRxiv - Biophysics 2020Quote: ... and secondary antibodies used were goat anti-mouse IgG-HRP (Cell Signalling Technology) and goat anti-rabbit IgG-HRP (Thermofisher). Densitometric analysis of the target bands was done in ImageJ (34 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated for 1 hr at room temperature in the dark with secondary antibodies Alexa488-conjugated goat anti-mouse IgG and Alexa546-conjugated goat anti-mouse IgG (Invitrogen). Nuclei were stained with DAPI (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2022Quote: ... Blots were washed 3x with PBS and probed with Alexa Fluor 680 Goat Anti Mouse and Goat anti-Rabbit IgG (H+L) Alexafluor Plus 800 secondary antibodies (1/2000, Invitrogen) in 1% casein/PBS and visualized using a LiCor Odyssey infrared imager.
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bioRxiv - Microbiology 2022Quote: ... Bound primary antibodies were detected using 1:500-1:2000 dilutions of goat anti-mouse or goat anti-rabbit IgG secondary antibodies conjugated with Alexa Fluor 488 or Alexa Fluor 594 (Invitrogen). Tachyzoite and HFF nuclei were labelled with DAPI (4’,6’-diamidino-2-phenylindole) ...
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bioRxiv - Microbiology 2022Quote: ... Reactive bands were visualised using goat anti-mouse IgG or goat anti-rabbit IgG secondary antibodies conjugated to horseradish peroxidase (Invitrogen) and the LiteAblot Plus Enhanced Chemiluminescent Substrate (EuroClone).
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: ... goat anti-mouse 488 or goat anti-rabbit 488 diluted in blocking buffer (A11034 and A32731, Thermo Fisher; 1:400) for 75 min at RT ...
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bioRxiv - Genetics 2022Quote: ... Secondary antibodies goat anti-mouse Alexa Fluor 488 (1:200) and goat anti-rabbit Alexa Fluor 568 from Life Technologies were used for visualization ...
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bioRxiv - Microbiology 2022Quote: ... For IFA secondary antibodies were Alexa Fluor 594 or Alexa Fluor 488-conjugated goat anti-rabbit and goat anti-rat (Invitrogen), both used at 1:2000 ...
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bioRxiv - Microbiology 2022Quote: ... Slides were incubated with primary antibodies followed by Alexa Fluor 488-conjugated goat anti-mouse secondary antibody or Alexa Fluor 594-conjugated goat anti-rabbit secondary antibody (diluted 1:400) (Invitrogen). The nuclei were stained with DAPI (10 μg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... Embryos were then washed 4 x 2 h in PBT and incubated overnight at 4°C in PBT supplemented with goat anti-mouse and goat anti-rabbit secondary antibodies coupled to Alexa Fluor 488 and 568 (Invitrogen). Embryos were then washed 4 x 2 h in PBT and gradually dehydrated in methanol ...
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bioRxiv - Developmental Biology 2020Quote: ... Secondary antibodies were goat anti-mouse IgG conjugated with Alexa 488 or 546 and goat anti-rabbit IgG conjugated with Alexa 546 (Invitrogen). Alexa 633–phalloidin at 1:300 (Thermo Fisher Scientific ...