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Citations for Thermo Fisher :
2201 - 2250 of 10000+ citations for Progesterone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: Single cells were sorted in 96-well PCR plates in 9 µl of RT/pre-amp mix from the CellsDirect One-Step qRT-PCR Kit (Life Technologies) and were kept at −80°C at least overnight ...
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bioRxiv - Microbiology 2021Quote: ... The resulting amplification products were verified by gel electrophoresis and cleaning and normalization of individual PCR products was performed with SequalPrep™ Normalization Plate (96) Kit (Invitrogen). The normalized PCR amplicons were mixed ...
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bioRxiv - Microbiology 2019Quote: ... The DNA amplicons in each PCR reaction was normalized using the SequalPrep Normalization Plate Kit (Thermo Fisher Scientific, catalog no. A1051001). The normalized reactions were pooled and quantified using the Kapa Biosystems Library qPCR MasterMix (ROX Low ...
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bioRxiv - Cell Biology 2019Quote: ... The incubation and cycling conditions were set as described in the kit and the plates were analysed in a StepOnePlus Real-Time PCR System (Thermo Scientific).
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bioRxiv - Pathology 2021Quote: ... Cells grown for 24h in 6-well plates were transfected with 1 μg of cDNA construct (Asic2b or t-Asic2b) using a Lipofectamine™ Plus kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... Samples were quantified in a 96-well plate reader using the Quant-IT DNA high sensitivity assay kit (Invitrogen, Cat #Q33232) according to the manufacturer’s protocol and pooled into 1 tube ...
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bioRxiv - Immunology 2020Quote: ... or luminal cells collected in vaginal washes were plated in 96-well plates and incubated with Live/Dead Fixable Aqua Dead Cell Stain kit (Molecular Probes) for 15 minutes at room temperature (RT ...
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bioRxiv - Genomics 2021Quote: Protein lysates derived from same plates used for metabolomics collection were assayed for total protein concentration with Pierce BCA Protein Assay Kit (ThermoFisher 23225) for normalizing raw metabolite values ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were plated into black 96-well clear bottom plates and analyzed using CyQuant cell proliferation assay kits (Thermo Scientific, c7026).
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bioRxiv - Immunology 2022Quote: ... Amplified linear DNA cassettes encoding monoclonal heavy and light chain genes were co-transfected into Expi293 cells in 96-well deep-well plates using the Expi293 Transfection Kit (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... A volume of 200 μL of milk samples were used as starting material for magneticbased DNA extraction using a 96-well plate and CORE kit in a KingFisher instrument (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Approximately two million colonies were scraped from plates and pooled and plasmid DNA was purified using PureLink® HiPure plasmid filter Midiprep kit (Invitrogen). The resulting plasmid library was transformed into TRy1392 pdr5∆ yeast strain ...
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bioRxiv - Cell Biology 2023Quote: Cells were seeded in 96 well plates (6055302, Revvity) and stained with Blue/Green ReadyProbes Cell Viability Imaging Kit (Invitrogen, R37609) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Calcium flux was measured in MNs seeded in a 96 well plate using the Fluo-4 NW Calcium Assay Kit (ThermoFisher, #F36206) per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: RNA was extracted from 8-μm snap frozen sections of distal femur and proximal tibial growth plates in Axin2CreERT2;R26RTdTomato mice using the Picopure RNA Isolation Kit (KIT0204, Thermo Fisher) as previously described.56 Microdissections of resting chondrocytes and proliferative chondrocytes were performed using a Leica LMD 7000 laser microdissection system (Leica Microsystems) ...
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ALS iPSC-derived microglia and motor neurons respond to astrocyte-targeted IL-10 and CCL2 modulationbioRxiv - Neuroscience 2023Quote: Calcium flux was measured in MNs seeded in a 96 well plate using the Fluo-4 NW Calcium Assay Kit (ThermoFisher, #F36206) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 20 clones from each plate were expanded and genomic DNA extracted by a GeneJet Genomic DNA Purification Kit (ThermoFisher Scientific, K0722) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... extracted from a single well of a 6-well plate using a mixture of RIPA lysis buffer and protease inhibitor then quantified using Pierce BCA kit (ThermoFisher Scientific), were separated via SDS-PAGE and subsequently transferred onto PVDF membranes ...
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bioRxiv - Molecular Biology 2023Quote: ... cell culture plates (12-well plates were Corning™ Costar™ cat# 3513 and 6-well plates were Thermo Fisher Scientific Nunc™ cat# 140675) were coated with GeltrexTM (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... cell culture plates (12-well plates were Corning™ Costar™ cat# 3513 and 6-well plates were Thermo Fisher Scientific Nunc™ cat# 140675) were coated with GeltrexTM (Gibco ...
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bioRxiv - Biochemistry 2022Quote: ... and 5 µg/mL and 5 units/mL penicillin-streptomycin (Gibco). One T175 flask of HEK293 cells of 70% confluency per condition was transiently transfected using 90 µg of EGFP-PABPi or EGFP-PABPi mut ...
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bioRxiv - Neuroscience 2021Quote: ... 5 U/ml penicillin and 5 U/ml streptomycin (Invitrogen, USA). Cells were plated on poly-D-lysine (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... using forward primer 5’-CACAGGAAGCCCTGGAAGCT and reverse primer 5’-GAGCAGGTCAGGTTTTTGGA (Invitrogen). SIGLEC22P was amplified similarly with forward primer 5’-GCACCTCAGAGTGGAAGGAC and reverse primer 5’-GAAGGGGTGACTGAGGTACA ...
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bioRxiv - Cell Biology 2021Quote: ... with forward primer 5’-GCACTTGCAGCCGGATTTTTGGATCCATAGCCAGGGCC and reverse primer 5’-GGCCCTGGCTATGGATCCAAAAATCCGGCTGCAAGTGC (Invitrogen) to generate the pcDNA3.1-KIR-CD33-V5/HIS vector ...
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bioRxiv - Biochemistry 2024Quote: hSSB2 was labeled with 5-IAF (5-iodoacetamido-fluorescein, Thermo Fisher) on the intrinsic cysteines ...
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bioRxiv - Cell Biology 2023Quote: ... and 5 µL of 5% ammonium persulfate (APS) (Thermo Scientific, 17874). 50 µl was added to a strip of parafilm in a humidity chamber on ice ...
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bioRxiv - Biophysics 2023Quote: ... samples were incubated with a 5 μM DRAQ-5 (Thermo Scientific) DNA staining buffer at 37° C for 30 minutes with agitation ...
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bioRxiv - Biochemistry 2024Quote: ... 0.3 × 5 mm trapping column (5 µm, 100 Å, Thermo Scientific) and analyzed by nanoLC-ESI-MS/MS analysis using a Thermo Ultimate 3000 RSLC nanoUPLC coupled online to an Orbitrap Exploris™ 240 mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... containing 5 unit/mL penicillin and 5 ug/mL streptomycin (Gibco).
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bioRxiv - Microbiology 2020Quote: ... and probe E_Sarbeco_P1 (5′-FAM-ACACTAGCCATCCTTACTGCGCTTCG-BBQ-3′) using the TaqPath 1-Step Multiplex Master Mix kit (Applied Biosystems) on a QuantStudio 5 real-time PCR system (Appiled Biosystems) ...
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bioRxiv - Developmental Biology 2021Quote: ... guide RNA for RBPMS2 (sequence 5’-GTCTTGCAGTGAGCTTGATC-3’) was synthesized using the T7 MegaShortScript transcription kit (Thermo Fisher Scientific). We previously described methods 28 to generate a doxycycline - inducible Cas9 line in the WTC-11 iPSC background (Coriell Institute) ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA of E12.5 neural tube tissue was extracted using the Ambion Purelink RNA mini kit according to the manufacturer’s instructions (ThermoFisher). Invitrogen Purelink DNase (ThermoFisher ...
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bioRxiv - Genetics 2020Quote: ... 1μl (equivalent to a 5:1 insert:vector ratio) was ligated into a blunt-ended TOPO vector (Zero Blunt TOPO PCR Cloning Kit, ThermoFisher) and transformed into E.coli ...
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bioRxiv - Systems Biology 2021Quote: ... A 1:5 dilution of this protein solution was quantified using a Pierce BCA protein assay kit (Thermo scientific). For analysis of the relative composition of amino acids ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μg of total RNA was treated with TURBO DNA-free™Kit (Invitrogen, Thermo Fisher Scientific, MA, USA) to remove any potential genomic DNA contaminants ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μg of total RNA was treated with TURBO DNA-free™Kit (Invitrogen, Thermo Fisher Scientific, MA, USA) to remove any potential genomic DNA contaminants ...
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bioRxiv - Microbiology 2022Quote: ... DNA was digested from 5-10µg of total RNA per sample using the TURBO DNA-free kit (ThermoFisher Scientific) and purified using RNA Clean and ConcentratorTM-5 kit ...
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bioRxiv - Physiology 2022Quote: Amplicons for dsRNAs were designed using the SnapDragon dsRNA design tool (https://www.flyrnai.org/snapdragon) and in vitro transcribed (IVT) using MEGAscript® T7 Transcription Kit (Cat# AMB1334-5, ThermoFisher). IVT reactions were carried out as per the protocol provided by the DRSC (available at ...
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bioRxiv - Plant Biology 2021Quote: The TSS of PlAMV sgRNA was detected in 5′ RACE analysis using a GeneRacer Kit (Invitrogen, Carlsbad, CA, USA), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Remaining cells were centrifuged for 5 minutes at 500 g before 100µL of extraction buffer added (Arcturus Picopure RNA kit, Thermofisher) and lysates frozen at −80°C.
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bioRxiv - Evolutionary Biology 2020Quote: ... The full structure of the NUKU2 transcript was determined with 5’ and 3’ RACE using the GeneRacer kit (Invitrogen), and testicle total RNA (Ambion ...
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bioRxiv - Neuroscience 2022Quote: ... 5-ethynyl-2’-deoxyuridine (EdU) was incubated using Click-iT™ Plus EdU Cell Proliferation Kit (Thermo Fisher Scientific) after DAPI staining ...
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bioRxiv - Microbiology 2022Quote: ... Messenger RNA (5 μg total RNA per sample) was enriched using a MICROBExpress bacterial mRNA purification kit (Thermo Fisher) and cDNA libraries containing unique barcodes generated from 100 ng mRNA using the Next UltraII Directional RNA Library Prep kit for Illumina (New England Biolabs) ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were loaded with 5 µM calcium-sensitive fluorescent dye Fluo-4AM (Direct Calcium Assay Kit, Invitrogen, USA) for 30 min at 37° C and washed for 15 min or 30 min at 37° C and then for 5 min at RT in basic solution (BS ...
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bioRxiv - Neuroscience 2024Quote: ... 5303 SPRN SPRN-5) was biotinylated using the EZ-Link NHS-PEG4 Biotinylation Kit (Thermo Scientific, catalog No. PI21455) and used at a concentration of 1µg/ml ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from 5×107 promastigotes with the help of the PureLink RNA mini kit (Invitrogen, USA). The RNA was treated with DNaseI (1U DNase I per 2 μg RNA at 37°C for 30 min ...
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MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylationbioRxiv - Molecular Biology 2023Quote: 5 µg of total RNA was used for poly(A)+ RNA purification with MicroPoly(A)Purist™ Kit (Ambion). Poly(A)+ RNA was fragmented at 70°C for 10 min in RNA Fragmentation buffer (Ambion) ...
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bioRxiv - Physiology 2023Quote: Amplicons for dsRNAs were designed using the SnapDragon dsRNA design tool (https://www.flyrnai.org/snapdragon) and in vitro transcribed (IVT) using MEGAscript® T7 Transcription Kit (Cat# AMB1334-5, ThermoFisher). IVT reactions were carried out as per the protocol provided by the DRSC (available at ...
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bioRxiv - Developmental Biology 2023Quote: ... 5 uL proteinase K mix from the ARCTURUS® PicoPure® DNA extraction kit (Applied Biosystems, Cat no. KIT0103) was added to each sample and they were incubated for 30 min at 65°C followed by 5 min at 95°C ...
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bioRxiv - Genomics 2023Quote: ... Fragmented DNA was purified using ZYMO DNA Clean & Concentrator™-5 kit and quantified using Qubit fluorometer (Life Technologies). Purified DNA was serial diluted with healthy donor cfDNA to generate libraries from 100% ...