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Citations for Thermo Fisher :
2201 - 2250 of 5144 citations for Parkin Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Non-specific binding to the membrane was blocked with Odyssey® PBS Blocking Buffer (Fisher Scientific NC9877369) and probed with the antibodies listed below ...
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bioRxiv - Cell Biology 2020Quote: ... The subsequently permeabilised cells were washed twice with PBS before blocking in 2% Goat serum (Life Technologies) and 1% BSA (Sigma).
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bioRxiv - Pathology 2021Quote: ... Membranes were blocked for 1 hour at RT using Pierce™ Clear Milk Blocking Buffer (ThermoFisher Scientific). Blots were incubated overnight at 4°C with mouse anti-FLAG tag mAB (Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2020Quote: ... the vitelline membrane was carefully removed and embryos were transferred to CAS blocking reagent (Invitrogen, cat. # 008120). For staining of sections ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained as follows: 1-blocking with 2 μg/mL FcBlock (anti mouse CD16/CD32, Invitrogen) for 1 h at RT in microscopy buffer (5% w/V BSA (Sigma) ...
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bioRxiv - Developmental Biology 2020Quote: ... we incubated samples in BODIPY® FL Phallacidin (1:40 dilution in blocking buffer, Thermo Fisher Scientific) at RT for 1 hour ...
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bioRxiv - Physiology 2021Quote: ... Sections were blocked with StartingBlock™ T20/phosphate buffered saline (PBS) Blocking Buffer (Thermo Scientific, Waltham, MA) before being incubated with primary antibodies (in 0.2% Triton X-100 ...
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bioRxiv - Immunology 2021Quote: ... The tissue was washed again as described before and incubated with blocking buffer-diluted Bodipy (Thermofisher # D3922) for 1h at room temp ...
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bioRxiv - Genomics 2021Quote: ... the samples were bathed in blocking buffer for 15 min before being stained by DRAQ5TM (Thermo Fisher) for 10 min ...
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bioRxiv - Cell Biology 2021Quote: ... Cross-absorbed secondary antibodies tagged with Alexa-fluorophores were diluted in blocking buffer (1:400, Invitrogen, Switzerland) and applied for 30 min at RT ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by incubation with secondary antibodies in blocking buffer and 1 μg/ml DAPI (Thermo Fisher Scientific) in blocking solution for 50 min at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... and blocked for 45 minutes using a blocking buffer containing DPBS with 5% goat serum (ThermoFisher #16210064) 0.05% sodium azide (Sigma #S2002 ...
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bioRxiv - Cell Biology 2022Quote: ... and washed 3 times with blocking buffer before mounting on glass slides with Prolong Gold (ThermoFisher Scientific). For immunoblotting ...
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bioRxiv - Cell Biology 2022Quote: ... then incubated with blocking buffer (10% normal goat serum–50062Z, Thermo Fisher; 0.5% BSA–BP1600, Thermo Fisher; 0.2% Triton X-100-T8787 ...
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bioRxiv - Bioengineering 2024Quote: ... for 1 hour before blocking with 3% (w/v) bovine serum albumin (BSA, Fisher Scientific, Cat # BP9703100). To detect ARP3 and GLUT4 ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were blocked with 1X BlockerTM FL 10X Fluorescent blocking buffer (Thermo Fisher Scientific, Cat # 37565) followed by incubation with the respective primary antibodies overnight at 4°C ...
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bioRxiv - Pathology 2024Quote: ... HT7 was the only anti-mouse antibody used and a mouse-on-mouse blocking agent (Invitrogen: R37621) was applied for 30 minutes post protein block washes to reduce noise from secondary antibody binding and an additional three 5-minute washes were added before primary antibody incubation ...
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bioRxiv - Neuroscience 2024Quote: ... BV-2 cells were fixed and sequentially incubated with Mouse-on-Mouse IgG Blocking Solution (Invitrogen R37621) and Navenci blocking solution at 37°C for 60 min each in a pre-heated humidified chamber ...
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bioRxiv - Immunology 2024Quote: ... The fixed slides were washed with PBS for twice and blocked with Blocking buffer (Thermo Fisher Scientific) for 1 h at room temperature ...
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bioRxiv - Immunology 2023Quote: ... 1% OVA in PBST for the second round and ELISA Blocker Blocking Buffer (Thermo Fisher Scientific, N502) for the third round ...
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bioRxiv - Microbiology 2023Quote: ... followed by destaining using 25 mM NaOH and blocking using 1x BlockerTM FL Fluorescent protein block (ThermoFisher). Primary antibodies used were Anti-GapDH (G9545 ...
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bioRxiv - Developmental Biology 2023Quote: ... oocytes were incubated with the secondary antibodies (1:500 in blocking solution, Alexa Fluor, Thermo Fisher Scientific) for 1 hour in dark ...
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bioRxiv - Neuroscience 2023Quote: ... Sections stained with mouse primary antibodies were blocked in ReadyProbes “Mouse on Mouse” IgG blocking solution (Thermofisher) for 1 hour before the initial blocking step ...
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bioRxiv - Immunology 2023Quote: ... was used at 1:8000 dilution in blocking solution together with 0.01 mg/ml DAPI (Thermo Fisher). The wells were washed with wash buffer and PBS ...
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bioRxiv - Genetics 2023Quote: ... 0.08% blocking reagent) containing 10 µL H2O2 0.3% and 2.5 µL of Alexa488 fluorochrome-labeled tyramides (Invitrogen). Sections were washed two times in PBS for 10 min at RT ...
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Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat productionbioRxiv - Bioengineering 2023Quote: ... and blocked for 45 minutes using a blocking buffer of DPBS with 5% goat serum (ThermoFisher #16210064) and 0.05% sodium azide (Sigma #S2002) ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were diluted in 7.5ml Intercept PBS blocking buffer with 0.1% Tween (SynGAP1; 1:1000dil, Invitrogen, PA1-046 ...
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bioRxiv - Neuroscience 2023Quote: ... incubated with Alexa 647 labeled donkey anti-rabbit secondary antibodies (1:250 in blocking solution; Invitrogen A32795TR) for 1 h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... A blocking solution was then added with 0.1% Triton-X-100 and 3% bovine serum albumin (Invitrogen). An antibody solution of MAP2 (1:2500 ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was blocked for 1 h at RT with nucleic acid detection blocking buffer (Life Technologies), then probed for 1 h at RT with streptavidin-HRP 1:5,000 (Genescript ...
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bioRxiv - Developmental Biology 2023Quote: ... and incubated in blocking solution with goat or donkey raised Alexa Fluor-conjugated secondary antibodies (Thermo Fisher), at room temperature for 24 hours ...
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bioRxiv - Microbiology 2024Quote: ... the wells were blocked with 200 μl of blocking buffer containing 1x PBS (ThermoFisher Scientific, MA, USA), 0.05% of Tween 20™ (ThermoFisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... and secondary antibodies in blocking buffer were added (1:200) [donkey Anti-Goat (Invitrogen; A11057 or A32814), Donkey Anti-Mouse (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... blocking with 1% BSA and staining with rhodamine-conjugated phalloidin following manufacturer’s instructions (Invitrogen, Waltham, MA, USA) and Hoechst (15 min ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were incubated in a blocking solution containing 5% goat serum (16210–064, Thermo Fisher Scientific), 3% bovine serum albumin (BAC62 ...
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bioRxiv - Microbiology 2024Quote: ... the protocol remained the same except as follows: Blocking was done in TBST + 7.5% goat serum (GIBCO). The primary antibody incubation step included both mouse anti-N-extein and rabbit anti-C-extein antibodies (polyclonal sera ...
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bioRxiv - Bioengineering 2024Quote: ... and acetylated tubulin staining: 2 mL blocking buffer containing 20 μL anti-Sox2 (Invitrogen, 14-9811-82), 20 μL anti-Myt1 (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were blocked in blocking buffer composed of 10% normal goat serum (NGS; Gibco, cat# 16210-064) and 0.3% Triton X-100 (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... The suspension for blocking and antibody incubations was 1x Tris buffered saline (Thermo Fisher Scientific, J60877.K3), with 0.1% Tween 20 (Merck ...
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bioRxiv - Cancer Biology 2021Quote: ... Peptides were loaded onto the trapping column (PepMap100, C18, 300 µm x 5 mm, Thermo Scientific) using partial loop injection with 2% acetonitrile (ACN) ...
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bioRxiv - Cancer Biology 2021Quote: ... fractionated into 8 fractions using the High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific 84868) according to the manufacturer protocol and dried ...
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bioRxiv - Cancer Biology 2021Quote: Peptide mixtures were separated by reversed-phase chromatography on an EASY-Spray column (Thermo Fisher Scientic), 25-cm long (inner diameter 75 μm ...
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bioRxiv - Biochemistry 2021Quote: ... with 15 µM Impβ mixed with 75 µM Rev-peptide and 1x SYPRO Orange (Molecular Probes) diluted in H2O ...
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bioRxiv - Cell Biology 2020Quote: ... Cleaved peptides were analyzed by nanoflow LC-ESI-MS/MS (Thermo Scientific LTQ Orbitrap Velos Pro). Peptide sequences were mapped using ProteinProspector and Mascot software packages.
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bioRxiv - Cell Biology 2020Quote: ... The isobaric labelling of the peptides was performed using 10-plex TMT reagents (Thermo Fisher Scientific).
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REEP4 is recruited to the inner nuclear membrane by ELYS and promotes nuclear pore complex formationbioRxiv - Cell Biology 2020Quote: Peptides were analyzed by C18 nanoflow reversed-phase HPLC (Dionex Ultimate 3000 RSLC nano, Thermo Scientific) combined with orbitrap mass spectrometer (Q Exactive HF Orbitrap ...
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bioRxiv - Microbiology 2021Quote: ... Peptides were loaded with the autosampler directly onto a 50 cm EASY-Spray C18 column (ThermoFisher). Peptides were eluted from the column using a Dionex Ultimate 3000 Nano LC system with a 14.8 min gradient from 1% Buffer B to 23 % Buffer B (100% acetonitrile ...
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bioRxiv - Developmental Biology 2020Quote: ... desalting and fractionation was performed with Pierce High pH Reversed-Phase Peptide Fractionation Kits (Thermo Scientific) to generate 8 fractions per sample ...
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bioRxiv - Developmental Biology 2022Quote: ... Peptide desalting was performed according to the manufacturer’s instructions (Pierce C18 Tips, Thermo Scientific, Waltham, MA). Desalted peptides were reconstituted in 0.1% formic acid in water and further separated into four fractions by strong cation exchange chromatography (SCX ...
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bioRxiv - Molecular Biology 2020Quote: ... Peptide desalting was performed according to the manufacturer’s instructions (Pierce C18 Tips, Thermo Scientific, Waltham, MA). Eluates were lyophilized and reconstituted in 11 µL of 5% acetonitrile and 2% formic acid in water ...