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Citations for Thermo Fisher :
2201 - 2250 of 10000+ citations for HER3 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... with 10% FBS (Nucleus Biologics, #AU FBS-500ml L1 HI) and 1% Penicillin-Streptomycin (Gibco, #15140122) and transferred to a new tube ...
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bioRxiv - Neuroscience 2024Quote: ... The buffer was then exchanged to the wash solution (10 % Hi-DiTM Formamide (Thermofisher scientific; 4311320) in 2x saline sodium citrate (SSC) ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatant fraction was incubated with 2 ml Ni-NTA His-Pur resin (Thermo Fisher, PI88222) for 1 h ...
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bioRxiv - Molecular Biology 2023Quote: Plasmid CassetteAv2_pBAD contains the CRISPR03 array cloned into the pBAD/Myc–His B backbone (Life Technologies).12 It was used to prepare CRISPR DNA substrates by PCR with primers MMB1Lead40-5 and MMB1crisp3-r1 using Phusion High-Fidelity DNA polymerase according to the manufacturer’s protocol (New England Biolabs or ThermoFisher) ...
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bioRxiv - Cell Biology 2023Quote: ... The inserts were released by Bam-HI and Eco-RI digestion and ligated to pENTR4 (Invitrogen; previously digested with the same restriction enzymes) ...
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bioRxiv - Cell Biology 2023Quote: ... The in vitro-translated proteins were then purified using His Pur cobalt spin columns (Thermo Scientific). For in vitro kinase assays ...
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bioRxiv - Biophysics 2022Quote: ... The His tagged RBD construct was purified from the culture supernatant of Expi293 cells (Thermo Fisher) that were transfected with the plasmid DNA using FectoPRO (Polyplus Inc.) ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were incubated 10 minutes at 4°C with Dynabeads™ His-Tag Isolation (Invitrogen, 10103D). After one wash with NLB buffer ...
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bioRxiv - Plant Biology 2023Quote: ... His-tagged AtKu70/80 was isolated from the supernatant using HisPur Ni-NTA resins (Thermo Fisher) and dialysed in 20 mM HEPES pH7.5 ...
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bioRxiv - Microbiology 2023Quote: ... brucei rRAP1-His at 10 µM was incubated with 10 mM SYPRO Orange dye (ThermoFisher Scientific), filtered before use with a 0.22 μm sterile filter (Fisher scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Cloning strategy for generating codon optimized SARS CoV-2 ORF7a in pCDNA-V5/His TOPO (Invitrogen) has been described previously (13) ...
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bioRxiv - Immunology 2023Quote: ... + 10% (v/v) HI-FBS + 100 U/ml penicillin-streptomycin (pen-strep, ThermoFisher Gibco™ #15140122) at 37°C ...
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bioRxiv - Immunology 2023Quote: ... + 10% (v/v) HI-FBS + 100 U/ml penicillin-streptomycin (pen-strep, ThermoFisher Gibco™ #15140122) at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... before preparation for sequencing with using Ion PGMTM Hi-QTM View Sequencing Kit (Thermo Fisher Scientific). The library was added to an Ion 318TM Chip Kit v2 BC (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... The digestion cocktail for RNase HI-negative samples was further supplemented with 120 U RNaseOUT (Invitrogen), while the digestion cocktail for RNase HI-positive samples were supplemented with 10 U recombinant Escherichia coli RNase HI (Invitrogen) ...
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bioRxiv - Synthetic Biology 2024Quote: ... His-tagged proteins were purified using 0.2 mL HisPur™ Ni-NTA Spin Columns from ThermoFisher according to the manufacturer’s protocol with the exception of carrying out 4 washing steps ...
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bioRxiv - Molecular Biology 2024Quote: ... the membrane was incubated with an anti-His Rabbit primary antibody (Thermo Scientific, Massachusetts, United States) (1:1000) ...
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bioRxiv - Genetics 2019Quote: HEK cells were transfected with plasmids encoding GFP-tagged Cdc42 variants using Calcium Phosphate transfection or Lipofectamine LTX Kit (Life Technologies).
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bioRxiv - Cell Biology 2022Quote: ... Transfer and packaging vectors were transfected into HEK 293T cells to produce lentiviruses using Lipofectamine 3000 reagent (ThermoFisher Scientific, MA, USA). Lentiviruses were harvested 48hr post transfection ...
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bioRxiv - Immunology 2022Quote: ... In-house expressed antibodies were made through co-transfection of heavy and light chain plasmids in Expi293 HEK cells (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: The human embryonic kidney (HEK) 293FT cells a high transfection efficiency isolate of the HEK293T cells were purchased from ThermoFisher (# R70007), and used without further authentication ...
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bioRxiv - Molecular Biology 2019Quote: HEK-293T cells (293T/17, ATCC) were maintained in high-glucose DMEM supplemented with 10% fetal bovine serum (FBS, Life Technologies). U2OS cells (ATCC ...
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bioRxiv - Genomics 2021Quote: ... Human embryonic kidney (HEK) 293T cells (CRL-11268, ATCC) were cultured in Dulbecco’s Modified Eagle’s Medium (11965-092, Gibco, Gaithersburg, MD, USA) supplemented with 10% (16000044 ...
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bioRxiv - Cell Biology 2021Quote: ... and pCMV-dR8.91 (Zufferey et al., 1997) (5 mg each) were transfected into HEK 293T/17 cells using Lipofectamine LTX (Thermo Fisher Scientific). Media containing lentivirus was collected 48 h and 72 h following transfection and filtered using a 13 mm syringe filter with a 0.45 µm PVDF membrane (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... envelope and one of the transfer plasmids were co-transfected into human embryonic kidney (HEK 293T) cells using Lipofectamine 3000 (ThermoFisher Scientific) according to the manufacturer’s lentiviral production protocol ...
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bioRxiv - Cancer Biology 2021Quote: A lentiviral pLKO.1-puro constructs targeting KSR1 and non-targeting control were transfected into HEK-293T cells using trans-lentiviral packaging system (ThermoFisher Scientific). The virus was collected ...
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bioRxiv - Physiology 2022Quote: ... ATCC) and human HEK-293T cells (catalog no. CRL-3216, ATCC) were grown in DMEM (catalog no. 31966-021, ThermoFisher Scientific) supplemented with 10% Fetal Bovine Serum (catalog no ...
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bioRxiv - Microbiology 2022Quote: ... The human anti-TNFα IgG1 monoclonal antibody was expressed in HEK-293F FreeStyle cell line expression system (Life Technologies, Carlsbad, CA) with co-transfection of vectors encoding p21 ...
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bioRxiv - Biophysics 2022Quote: 106 HEK cells were plated in 6-well plates and transfected with the indicated plasmids with Lipofectamine 2000™ reagent (Invitrogen). 24 hours after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Flag-CTPS1H355 and TRE-promoter driven myc-IMPDH2 and myc-IMPDH2Y12A were delivered into HEK 293T or HeLa cells with TurboFect™ transfection reagent (R0532, ThermoFisher). The expression of myc-IMPDH2 was turn off with culture medium containing 1 μg/ml doxycycline (ab141091 ...
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bioRxiv - Microbiology 2020Quote: ... New York University) and a pcDNA3.1 plasmid expressing the SARS-CoV2 glycoprotein into HEK 293T cells using Lipofectamine 3000 (Thermo Fisher Scientific). The supernatant was collected 48 h after transfection ...
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bioRxiv - Molecular Biology 2020Quote: Human embryonic kidney (HEK) 293T cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Thermo Fisher, Waltham, MA, USA; Cat#10566016) supplemented with 10% fetal calf serum (FBS ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells (INS-1 832/13 and HEK-293T) were seeded onto poly-D-lysine coated 8-well chambered cover glasses (Lab-Tek, Thermo Scientific) at a cell density of 70,000 cells/cm2 ...
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bioRxiv - Cell Biology 2021Quote: ... Expression characteristics of each plasmid were evaluated in HEK 293T cells by transient transfection using Lipofectamine™ 3000 (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... Lenti-X Human Embryonic Kidney (HEK) cells were transfected with the sgRNA plasmids using Lipofectamine 3000 (Life Technologies, Cat. No. L3000150), then cultured for 2-3 days in the following media ...
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bioRxiv - Immunology 2022Quote: ... HepG2 and HEK 293T cell lines were cultured in Dulbecco’s modified eagle medium (DMEM high glucose, Life Technologies, Carlsbad, CA, USA), supplemented with 10 % (v/v ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... SNAP-tagged human β2-adrenoceptor or human EP2 receptors fused at the C terminus to a thermostable Nanoluciferase (Hek-ssβ2-AR-tsNluc) using Lipofectamine 3000 reagent (Invitrogen, Paisley, UK). Original SNAP tag and Nanoluciferase sequences were from NEB (Hitchen UK ...
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bioRxiv - Synthetic Biology 2024Quote: HEK 293T cells (UCSF Cell Culture Facility) were cultured in medium consisting of Dulbecco’s Modified Eagle Medium (DMEM) (Gibco #10569-010) and 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... The pLV vectors were co-transfected with packaging plasmids (Celecta #CPCP-K2A) into HEK 293T cells using Lipofectamine 3000 reagent (Invitrogen #L3000001) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: Protein lysates from HEK cells or two-month-old mice frontal cortex were prepared using Pierce IP Lysis Buffer (Thermo Scientific) supplemented with cOmplete EDTA-free Protease Inhibitor Cocktail (Millipore Sigma ...
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bioRxiv - Molecular Biology 2024Quote: Luciferase emissions from transfected Hek-Luc cells were measured using the Pierce® Firefly Luciferase Glow Assay Kit (Thermo Fisher Scientific). To initiate cell lysis ...
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bioRxiv - Neuroscience 2024Quote: ... Both HEK cells and astrocytes were transfected the day after plating with 500 ng of plasmid DNA using lipofectamine 2000 (Invitrogen #11668019) and OptiMEM (Life Technologies #31985-070) ...
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bioRxiv - Microbiology 2024Quote: HEK 293T cells sorted as described in ‘Flow Cytometry’ below were counted using the Countess II Cell Counter (Thermo Fisher Scientific) and cell samples were diluted such that all conditions contained the same cell numbers as input ...
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bioRxiv - Cancer Biology 2024Quote: ... lentiviral vector DNA was transfected with psPAX2 and pMD2.G packaging plasmids into HEK-293FT cells using Lipofectamine 2000 transfection reagent (Invitrogen, 11668019) and D10 media lacking antibiotics ...
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bioRxiv - Cell Biology 2024Quote: Human FLAG-ATGL (Uniprot ID: Q96AD5) was expressed in 20 15-cm dishes of HEK 293T cells were cultured in DMEM (ThermoFisher Scientific) with 5% FBS and then transiently transfected with a solution of 1 µg mL-1 plasmid DNA and 1 mg mL−1 PEI ...
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bioRxiv - Immunology 2023Quote: ... Plasmids were transiently transfected in HEK-293T cells using polyethyleneimine (PEI, PolyAR) in a 1:2.5 DNA:PEI ratio in OptiMem medium (Thermo Fisher Scientific, 22600050). The supernatant containing recombinant proteins was harvested 72 h post-transfection and centrifuged at 10,000 g for 20 min at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... HEK-293T cells and HeLa cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM; Thermo Fisher Scientific, Waltham, MA, United States) supplemented with 10% FBS (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... commercially synthesized RNA with m6A methylation (Sequence: GGAAAGGA(m6A)CUAAAAA) and the corresponding unmethylated RNA were transfected into HEK-293T cells by lipofectamine 2000 (Invitrogen, 11668019), respectively ...
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bioRxiv - Genomics 2023Quote: ... Two 15-cm dishes were each seeded with 1.5×107 HEK 293T cells (ATCC, CRL-3216) in 25 ml DMEM complete medium: DMEM (Gibco, 11965-092) supplemented with 10% FBS (VWR ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK-293T cells for viral vectors production were cultured in DMEM high-glucose (w/ 4.5 g/l; #11995065; Thermo Fisher Scientific) with 10% heat inactivated FBS ...