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Citations for Thermo Fisher :
2201 - 2250 of 10000+ citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... cDNA was diluted 1:20 and amplified with PowerUp SYBR Green Master Mix (Applied Biosystems, A25778) using a ViiA-7 Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Cell Biology 2022Quote: ... pre-complexed for > 20 min with 1 μg DNA and 3 μg lipofectamine 2000 (ThermoFisher 11668019); DNA and lipofectamine were each diluted to 100 μl before combining ...
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bioRxiv - Immunology 2022Quote: ... 1 mM Na3VO4 and 20 mM NaF) supplemented with 2 U/ml DNase I (Invitrogen, AM2222), protease and phosphatase inhibitors ...
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bioRxiv - Microbiology 2022Quote: ... M(GFP)NE or M(GFP)NES were diluted (1:20) in transparent DMEM Solution (Gibco) and put into contact with the live cells ...
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bioRxiv - Neuroscience 2022Quote: ... and muscle-specific expression gateway entry clones of mbl-1 transgene (Thermo Fisher Scientific; K2500-20), the MBL-1 cDNA was single-site LR recombined with pCZGY66 (Prgf-1 destination vector) ...
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bioRxiv - Cell Biology 2024Quote: ... pre-complexed for > 20 min with 1 μg DNA and 3 μg lipofectamine 2000 (ThermoFisher 11668019); DNA and lipofectamine were each diluted to 100 μl before combining ...
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bioRxiv - Bioengineering 2024Quote: ... Digested ssDNA product was then mixed with 1 μl 20 mg/mL glycogen (Thermo Scientific, R0561) and 1/3 volume 10 M ammonium acetate to perform EtOH precipitation ...
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bioRxiv - Microbiology 2023Quote: ... The gel was stained for 20 minutes with 1:10,000 SYTO™ 60 stain (Invitrogen™) before subsequent visualisation on a Odyssey® CLx (LI-COR) ...
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bioRxiv - Microbiology 2022Quote: ... TIRF and STORM samples were incubated with 1 :20 dilution of phalloidin 647 Alexa Fluor (Invitrogen) overnight at 4 degree ...
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bioRxiv - Molecular Biology 2023Quote: ... and a 20-minute incubation with 1:200 tyramide AF555 (Thermo Fisher Scientific, catalog #: B-40957).
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bioRxiv - Molecular Biology 2023Quote: ... followed by addition of 2.8 μl of 20 mg ml−1 proteinase K (Thermo Fisher Scientific) and 1 h incubation at 55 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 7.4 0.15 M NaCl 0.1% Tween 20) with 1:100 protease inhibitor (78442, Thermo Scientific). The lysates were denatured by boiling ...
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Induction of PARP7 Creates a Vulnerability for Growth Inhibition by RBN2397 in Prostate Cancer CellsbioRxiv - Cancer Biology 2023Quote: PC3-AR cells were transfected with 20 nM of Negative Control siRNA #1 (siCon, Ambion AM4635), siPARP7 (sense strand 5’-AAUACUCUCAUCGAACGGAAGTT-3’ ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR was performed in 20 µl reactions containing 1 × Phusion HF buffer (Thermo Fisher Scientific, USA); 0.2 mM dNTPs (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... and 1/20 volume of 1M (in DMSO) Alexa Fluor 488 ester (Molecular Probes, Cat# A20000). A drop of premixed gel (∼50 μL ...
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bioRxiv - Cell Biology 2023Quote: ... Immunostaining for Scribble was performed using an anti-Scrib primary antibody (1:20, Invitrogen PA5-54821) and 18 nm gold-labeled secondary antibody.
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bioRxiv - Cancer Biology 2024Quote: ... 20 ng/ml bFGF and 1% penicillin and streptomycin and 50 µg/ml Gentamicin (Gibco, 15750060). PDX derived cells were plated in 6-well ULA plates (Corning ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 mM glucose (pH 7.3) that contained 0.02% Pluronic-F127 (P3000MP, ThermoFisher Scientific, 20% (w/v) solution in DMSO ...
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bioRxiv - Immunology 2024Quote: ... Bacteria is then diluted 1:20 into 10 mL DMEM (Cat# 11995-065 ThermoFisher Waltham, MA) or LB with increasing concentrations of CP96345 or DMSO and cultured at 37°C for 3 hours 200 RPM ...
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bioRxiv - Neuroscience 2023Quote: ... 20 μl Pluronic F-127 (20% w/v in DMSO, Thermo Fisher Scientific), and 60 μl sterile phosphate-buffered saline (PBS) ...
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bioRxiv - Cell Biology 2020Quote: ... 0.1% Tween-20 for 10 min and incubated with primary antibody (1:500 mouse α8-oxoG, QED Bioscience; 1:5000 rabbit αGFP, ThermoFisher, 1:5000 mouse αHA, Covance) overnight at 4°C in a humidity chamber ...
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bioRxiv - Microbiology 2021Quote: ... the purified PCR products were normalized to equimolar concentration with the SequalPrep Normalization Plate Kit (Thermo Fisher Scientific) and the DNA library was sequenced on the Illumina MiSeq next generation sequencing system (Illumina Inc. ...
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Hypoxia-mediated suppression of pyruvate carboxylase drives tumor microenvironment immunosuppressionbioRxiv - Cancer Biology 2022Quote: ... Labeled cDNA was hybridized to the plate using GeneTitan Hybridization Wash and Stain Kit for WT Arrays (Affymetrix).
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bioRxiv - Molecular Biology 2022Quote: ... The same plate was used for evaluating SOD (Superoxide Dismutase Colorimetric Activity Kit – Thermo Fisher Scientific – ref EIASODC) and CAT (Catalase Colorimetric Activity Kit –Thermo Fisher Scientific – ref EIACATC ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products were normalized with the SequalPrep Normalization Plate Kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) and eluted in 20 μL elution buffer ...
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bioRxiv - Immunology 2023Quote: ... CpG content was measured in a 96 well plate using Ribogreen™ fluorescence staining kit from Thermo Fisher and a SpectraMax i3 plate reader (Molecular devices ...
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bioRxiv - Microbiology 2023Quote: ... Duplicate PCRs were combined prior to clean up and normalization with a SequalPrep Normalization Plate kit (ThermoFisher Scientific) following manufacturer instructions using 25 µL of PCR product per sample ...
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bioRxiv - Immunology 2023Quote: ... CpG content was measured in a 96 well plate using Ribogreen™ fluorescence staining kit from Thermo Fisher and a SpectraMax i3 plate reader (Molecular devices ...
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bioRxiv - Microbiology 2023Quote: ... triplicate first-step PCR reactions were pooled and purified and normalised with the SequalPrep Normalisation Plate Kit (Invitrogen) following the manufacturers’ instructions ...
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bioRxiv - Physiology 2024Quote: ... Protein concentration was quantified on a plate reader using the Pierce BCA protein assay kit (#23225, Thermo Scientific). Gel lanes were loaded with 30 µg of total protein ...
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bioRxiv - Immunology 2021Quote: ... 5×105 murine or human T cells have been stimulated at 1:1 ratio in 48well plate in presence of Sulfate Latex 4% w/v 5µm Beads (Molecular Probes S37227). For each experiment ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cells were washed four times with PBS (10min at RT for each wash) and then ECL (1:1) was added to detect the signal by the plate reader (Varioskan Flash, Thermo Scientific). The signal from the samples ...
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bioRxiv - Immunology 2020Quote: ... monocytes were plated in low-serum media (1% FBS) for 1-2 hours in 12-well Nunc-coated plates (ThermoFisher Scientific) to promote adherence ...
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bioRxiv - Evolutionary Biology 2022Quote: ... then mixed 1:1 and diluted 400-fold in 200 µl of fresh LB in 96-well plates (Thermo Scientific, Denmark), and incubated during 24 in the same conditions ...
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bioRxiv - Cancer Biology 2022Quote: Organoids were collected from culture plates and dissociated into single cells using a 1:1 mixture of TrypLE Express (Gibco, 12604013) & Accutase (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... and THP-1 cells (100 μl of 1 x 106 cells/mL) were seeded in 96 well tissue culture plate (Nunc™, Thermofisher) for 24 hours using DMEM and RPMI media respectively supplemented with 10% heat inactivated FBS in 5% CO2 incubator at 37°C ...
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bioRxiv - Immunology 2020Quote: ... were cultured for 16 h in each well of a 12 well plate in serum-free medium (MEM Glutamax, containing 1% v/v Penicillin-Streptomycin and 1%v/v sodium pyruvate [Gibco, Thermofisher]). SARS-CoV-2 clinical samples (100 TCID50/ well ...
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bioRxiv - Neuroscience 2023Quote: ... Rosettes were plated on growth factor reduced matrigel coated 6 well plates in ‘pNPC medium’ composed of a 1:1 mixture of Neurobasal (ThermoFisher Scientific) and DMEM/F12 ...
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bioRxiv - Neuroscience 2024Quote: ... coated organoids were returned to the 24-well ultra-low-attachment plates with differentiation media without vitamin A [1:1 mixture of DMEM/F12 and Neurobasal (Gibco, USA), 0.5% N2 supplement ...
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bioRxiv - Cancer Biology 2023Quote: ... the cell pellet was resuspended and cultivated in a 24-well plate with a 1:1 medium of defined Keratinocyte-SFM (Thermo Fisher) and complete embryonic fibroblast medium (Cell Biologics) ...
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bioRxiv - Developmental Biology 2020Quote: ... 5um serial sections were generated and used for immunofluorescence with primary antibodies recognizing sarcomeric myosin (1:20, DSHB MF20) and Alexa Fluor-conjugated secondary antibodies (1:1000, Thermo Fisher Scientific). Antigen retrieval was performed on all sections with 10mM sodium citrate buffer ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were washed in 0.2% Tween-20/1× PBS and stained with 200 µL of 1:200 Alexa-488 conjugated donkey anti-rabbit secondary antibody (Invitrogen, Cat. # A21206) for 1 hour ...
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bioRxiv - Cancer Biology 2019Quote: ... supplemented with 10% (KG-1, HL-60) or 20 % (MOLM-14, KASUMI-1) heat inactivated fetal bovine serum (FBS, Gibco, Thermo Fisher Scientific), 2 mM L-glutamine (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2024Quote: Larvae were paralyzed by a 1 minute immersion in α-bungarotoxin (20 µl of 1 mg/ml solution in system water; B1601, ThermoFisher Scientific), followed by washing in fresh system water (Duboué et al. ...
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bioRxiv - Neuroscience 2023Quote: ... at which time they were paralyzed by a 1 min immersion in α-bungarotoxin (20 µl of 1 mg/ml solution in system water, B1601, ThermoFisher Scientific) and flushed with fresh system water ...
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bioRxiv - Developmental Biology 2023Quote: ... 20% Knockout Serum Replacement, 1% Glutamax, 1% Non-Essential Amino Acids, 0.1% ß-Mercaptoethanol, and 8 ng/mL bFGF, Thermo Fisher Scientific), and passaged weekly by manual disruption of 6-10 representative colonies ...
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bioRxiv - Cell Biology 2023Quote: ... and 20% sucrose-PBS and then incubated overnight at 4°C in a 1:1 solution of 20% sucrose and OCT compound (Fisher Scientific: #23730571). The next day ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were washed by PBS with 0.1% Tween 20 (PBST) (three times for 5 min each) and incubated with Alexa Fluor secondary antibodies (1:500) (Thermofisher, #A-11036) for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: ... Purified peptides were diluted to 0.1 μg μl−1 and 20 μl (2 μg) was injected onto a Dionex UltiMate 3000 RSLCnano LC system (Thermo Fisher Scientific) with an additional RSLCnano pump run in online two-dimensional active modulation mode coupled to a Thermo Fusion Orbitrap mass spectrometer.
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bioRxiv - Molecular Biology 2019Quote: ... for 20 min at RT and reverse transcribed using the Maxima First-Strand cDNA Synthesis Kit (K1671, ThermoFisher Scientific) according to the manufacturer’s protocol ...