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Citations for Thermo Fisher :
2151 - 2200 of 6821 citations for Recombinant Human FABP6 None tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Secreted dimeric C-terminally Fc-tagged MDGA1 stably expressed in HEK293T cells was collected in serum-free Opti-MEM (Thermo Fisher Scientific, Inc.). Fc-tagged MDGA1 protein was run on an affinity column packed with Protein-G Plus Agarose fast flow resin (Pierce ...
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bioRxiv - Cell Biology 2021Quote: ... The second step was the separation of mCherry-zyxin or EGFP-VASP from 6xHis-MBP and the removal of the His-tagged TEV protease by Ni-NTA chromatography (HisPur™ Ni-NTA Resin, Thermo Scientific: 88222). First ...
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bioRxiv - Cell Biology 2021Quote: ... Alexa-Fluor-488- or Alexa-Fluor-568-tagged donkey anti-mouse- or anti-rabbit-immunoglobulin antibodies (A32766, A32790, A10042, A10037, Molecular Probes, 1:200) and Alexa-Fluor-633 goat anti mouse (A-21050 ...
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bioRxiv - Biophysics 2022Quote: ... anti-His tag antibody molecules were tagged with the dye Alexa Fluor® 647 NHS Ester (Succinimidyl Ester, ThermoFisher SCIENTIFIC, Catalog number: A20006) using the same procedure as followed in our previous paper.(11 ...
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bioRxiv - Physiology 2022Quote: ... HPAECs were transfected with either control plasmids (empty vector) or 1μg of HA-ubiquitin plasmids (HA-tagged Ubi) using Lipofectamine 3000 (Thermo fisher Scientific, PA, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed three times with 1X PBS and incubated with suitable fluorophore tagged secondary antibodies (1: 2000, Invitrogen, San Diego, CA, USA). Cells were washed with 1X PBS before mounting ...
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bioRxiv - Molecular Biology 2024Quote: ... 2008) N-terminally tagged with the V5 epitope was synthesized as a DNA string (GeneArt Strings DNA Fragments, Thermo Fisher Scientific, Schwerte, Germany) and cloned into pcDNA3.1 to obtain pcDNA3.1-V5-RHEB-Q64L ...
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bioRxiv - Molecular Biology 2024Quote: T7 double stranded DNAs (T7-dsDNAs) were amplified by T7-tagged primer pairs (Table 2) by Phire Hot Start II PCR Master Mix kit (Thermo Fisher, Cat # F125L) using proper plasmids as templates ...
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bioRxiv - Microbiology 2024Quote: ... Detection of the murine-human chimera 72A1 detection was performed using goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody Alexa Fluor™ 488 tagged (Invitrogen #A-11001).
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bioRxiv - Cancer Biology 2023Quote: ... Tissues were further incubated for 2 h at room temperature with HRP-tagged secondary antibody of goat anti-mouse (1:1000) (Life Technologies, Thermo Scientific, USA). The sections were then washed with TBST three times ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed twice with phosphate-buffered saline for 5 minutes and incubated with Alexa Fluor 555-tagged donkey-anti-goat (Molecular Probes, 1:1000) and Alexa Fluor 647-tagged donkey-anti-rabbit (Molecular Probes ...
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bioRxiv - Developmental Biology 2023Quote: ... the samples were washed with 0.1% PBS-TX three times and treated with Alexa Fluor 488-tagged goat-anti-rabbit IgG antibodies (Thermo Fisher, Cat # A-11008), diluted 1:800 in 0.1% PBS-TX for 6–12 h at 4 °C ...
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bioRxiv - Bioengineering 2023Quote: ... 1 x 107 pfu of mRFP-VP26 tagged virions or 1 x 108 of Yellow-Green fluorescent tagged nanoparticles (160 nm, FluoSpheres™ Carboxylate-Modified Microspheres Catalog #F8811, Thermo Fisher Scientific) were added to the dish ...
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bioRxiv - Cell Biology 2024Quote: HEK293T or HeLa Kyoto cells expressing the indicated tagged proteins were washed twice with ice-cold PBS and lysed in M-PER mammalian protein extraction reagent (Thermo Fisher Scientific, 78501) containing protease and phosphatase inhibitors ...
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bioRxiv - Microbiology 2019Quote: Human IL-18 and TNFα were measured with the human IL-18 ELISA Kit (MBL, Woburn, Massachusetts) and human TNFα ELISA Kit (ThermoFisher, Waltham, Massachusetts) according to the manufacturers’ instructions using cell culture supernatant at a 1:5 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... Human brain samples classified into healthy (n=47) and AD (n=32) were processed by array (Affymetrix Human Gene 1.0 ST Array)53 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Telomerase-immortalized human neonatal foreskin keratinocytes (N-TERTs) (21) were cultured in EpiLife medium containing human keratinocyte growth supplement (HKGS) (Thermo Fisher Scientific) and penicillin/streptomycin ...
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bioRxiv - Immunology 2022Quote: Single cell suspensions of human PBMC were stimulated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (ThermoFisher Scientific) as per manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... 0.01ug/ml soluble human anti-CD3 (R&D clone UCHT1) and 0.1 ug/ml soluble human anti-CD28 (Life Technologies; clone CD28.2) at a concentration of 1×106 cells/ml in RPMI-1640 (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... SH-SY5Y human neuroblastoma (ECACC) and RH-30 human rhabdomyosarcoma (kindly donated by Marc-David Ruepp) were maintained in DMEM (Thermo Fisher Scientific), supplemented with 10% FBS (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: Cell lines used in this study were THP-1 (human monocytic leukemia) cells, HeLa (Henrietta Lacks, human cervical carcinoma cells, CCL2) and HEK293T Flp-In T-REx 293 (Thermo Fisher Scientific). THP-1 cells were cultured in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Immunology 2021Quote: ... CD4+ and CD8+ T cells were isolated by negative selection with the Dynabeads Untouched Human CD4 T Cells Kit and the Dynabeads Untouched Human CD8 T Cells Kit (Invitrogen, Thermo Fisher Scientific) respectively ...
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bioRxiv - Immunology 2021Quote: ... Human primary CD8+ T Cells were negatively isolated from PBMCs using Dynabeads™ Untouched™ Human CD8 T Cells Kit (ThermoFisher Scientific) or Human CD8+ T Cell Isolation Kit (Miltenyi Biotec) ...
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bioRxiv - Immunology 2021Quote: ... and the biotinylated secondary anti-human IgG (ref. B1140-1ML, MilliporeSigma, St. Louis, USA)] and the anti-human IgE (ref. A18803, Invitrogen, Waltham, USA) antibodies were added at 1:1250 and 1:100 dilutions ...
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bioRxiv - Cell Biology 2022Quote: ... were prepared from fresh human umbilical veins as previously described (Barbieri et al., 1981) and maintained in Human Endothelial SFM (Gibco™, Life Technologies Corporation ...
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bioRxiv - Cell Biology 2024Quote: ... Human kidney cells (HKC) and human embryonic kidney cells (HEK293T) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, ThermoFisher Scientific, Cat. # 11965126). Mouse embryonic fibroblasts (MEF ...
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bioRxiv - Immunology 2024Quote: ... The cells were then washed in PBS and stained with a mix of secondary antibodies anti-human IgG-Fc-AF647 (1:600) and anti-human IgA-Alpha-Chain-AF488 (1:200) (Thermo Fisher Scientific) for 30 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... 0.01 µg/ml soluble human anti-CD3 (R & D; clone UCHT1) and 0.1 µg/ml soluble human anti-CD28 (Life Technologies; clone CD28.2) for 3 days before infection ...
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bioRxiv - Molecular Biology 2023Quote: ... All hFRG mice reported in this study were engrafted with human and non-human primate hepatocytes from the same donors (Caucasian, 15-month-old donor, Lonza, #HUM181791; ThermoFisher, #CY409, respectively).
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bioRxiv - Immunology 2023Quote: Human pluripotent stem cells (PSCs) were generated from anonymized human PBMCs based on published protocols115 using Sendai viral vectors (Thermo Fisher, A16517) driving high expression of pluripotency markers NANOG and OCT4 ...
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bioRxiv - Biochemistry 2024Quote: ... The preparations of insect cell microsomes containing human CYP2A6 (lot 2520331) and CYP2D6 (lot 2350106) along with human CPR and cytochrome b5 (Baculosomes®) were procured from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Genomics 2024Quote: Dataset 3: This dataset includes the Human Origins SNP Array genotypes (2,583 present-day individuals genotyped on the Affymetrix Human Origins Array) merged with ancient individuals from SW Asia and S Europe called across a total of 616,938 autosomal SNPs (Lazaridis et al. ...
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bioRxiv - Developmental Biology 2021Quote: Human iPSCs were cultured in StemPro hESC SFM (Gibco) supplemented with 20ng/ml bFGF (R&D ...
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bioRxiv - Genomics 2021Quote: ... and HLA-DR/human MHC class II antibodies (Invitrogen Dynabeads® ...
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bioRxiv - Microbiology 2021Quote: ... Human hepatocellular carcinoma cells (Huh-7; Thermo Fisher Scientific) and African green monkey kidney cells (Vero E6 ...
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bioRxiv - Immunology 2022Quote: ... and CD3/CD28 Human T-activator Dynabeads (Thermo Fisher) at a 1:1 bead to cell ratio ...
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bioRxiv - Molecular Biology 2020Quote: ... Commercial pooled RNA (Quantigene Human Reference, QS0639, Life Technologies) and pooled human male DNA (G3041 ...
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bioRxiv - Molecular Biology 2021Quote: ... MEFs and human fibroblasts were cultured in DMEM (Invitrogen) supplemented with 10% FBS (Atlanta Biologicals ...
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Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytesbioRxiv - Molecular Biology 2021Quote: ... and Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher) at 1 bead per cell ...
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bioRxiv - Genomics 2020Quote: ... or Mouse anti-human GAPDH monoclonal (MA5-15738, Invitrogen). The secondary antibody was HRP conjugated Goat anti-Rabbit (NA934 ...
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bioRxiv - Cell Biology 2019Quote: Unlabeled LDL (1 mg) from human plasma (Thermo Scientific) was incubated with 50 nmol cholesterol (Top-Fluor cholesterol ...
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bioRxiv - Cancer Biology 2019Quote: ... rabbit anti-human CD133 (Thermo Fisher Scientific, 1:1000) or rabbit anti-human Vimentin (Proteintech Group ...
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bioRxiv - Physiology 2019Quote: ... Antibodies: anti-human endoglin (Ref. H300; Thermo Fisher Scientific) and anti-mouse β-actin (Ref ...
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bioRxiv - Cancer Biology 2019Quote: ... Rabbit anti-human-Ki67 (1:20, Thermo Fisher Scientific), Rabbit anti-Chromogranin A (1:500 ...
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bioRxiv - Cancer Biology 2021Quote: ... a secondary goat anti-human Alexa Fluor-555 (Invitrogen) was used as the target sites were bound with anti-human TCB after administration ...
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bioRxiv - Immunology 2021Quote: ... and 10% heat-inactivated human AB-positive serum (Invitrogen) at 37°C in 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... purified using CaptureSelect LC-lambda affinity matrix (human) (ThermoFisher), and concentrated with amicon ultra centrifugal filter tubes (30K MWCO ...
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bioRxiv - Bioengineering 2021Quote: ... 5 ng/ml human epithelial growth factor (Life Technologies), 0.5 ng/ml bFGF (Life Technologies) ...
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bioRxiv - Bioengineering 2021Quote: ... free style HEK-293 human embryonic kidney cells (Invitrogen) were transfected with a mammalian expression vector encoding for the human IgG1 kappa light chain and full-length heavy chain sequences (based on variable sequences of cetuximab ...
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bioRxiv - Cancer Biology 2021Quote: ... were transfected into the human 293FT (Thermo Fisher Scientific) cell line using X-tremeGENE 9 DNA transfection reagent (Roche) ...