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Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for Mouse Bcl 2 Modifying Factor BMF ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and developed with 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Scientific™). For primary G8 T cells ...
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bioRxiv - Microbiology 2023Quote: ... and 50 mL of 1-Step Ultra TMB-ELISA was added (ThermoFisher Cat. #34028). Following a 12 to 15-min incubation ...
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bioRxiv - Neuroscience 2023Quote: ... then exposed with 100µL 1-step ultra TMB-ELISA substrate solution (Thermo Scientific; 34028) for 5 minutes ...
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bioRxiv - Immunology 2023Quote: ... 100 μL of room temperature 1-Step Ultra TMB-ELISA Substrate Solution (ThermoFisher #34028) was added to each well and the plate was incubated at 25 °C for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... Plates were developed with 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific) for 10-15 minutes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Antibodies and other reagents for immunofluorescence and ELISA experiments were obtained from Thermo Fisher Scientific (Spain) ...
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bioRxiv - Immunology 2023Quote: ... ELISA plates were developed with the TMB substrate (1-Step Turbo-TMB, Thermo Fisher) for six minutes and terminated with sulfuric acid (2M) ...
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bioRxiv - Cell Biology 2024Quote: ... ELISA absorbance was measured at 450nm and normalized to total DNA content (SYBRGreen; Invitrogen) using an absorbance/fluorescence microplate reader (BioTek) ...
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bioRxiv - Immunology 2024Quote: Indirect ELISA was performed using Maxisorp 96-well microtiter plates (Thermo Scientific, Ref. 442404) coated with 50 µL of a 2 µg/mL solution of the rPvs48/45 protein solution overnight at 4°C ...
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bioRxiv - Physiology 2021Quote: ... Samples (2 μl) were run on Qubit 3.0 using the Qubit RNA HS Kit (Invitrogen) to determine RNA quantification ...
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bioRxiv - Physiology 2021Quote: ... cDNA was synthesized using 2 ug RNA with RevertAid RT Kit (Thermofisher, catalog number K1691). Maxima SYBR Green/ROX qPCR Master Mix (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... We used 2 µg RNA for cDNA synthesis using a RevertAid cDNA synthesis kit (ThermoFisher) and measured gene expression using qRT-PCR (BioRad CFX system) ...
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bioRxiv - Immunology 2022Quote: Caspase-3 activity was determined using EnzChek™ Caspase-3 Assay Kit #2 (Thermo Fisher).
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bioRxiv - Molecular Biology 2020Quote: ... incorporation for 2 hours and detection (Click-iT EdU Alexa Fluor 488 Imaging Kit, Invitrogen).
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bioRxiv - Zoology 2019Quote: ... Calcium calibration was performed using the Fura-2 calcium calibration kit (Cat. Nr. F6774, Invitrogen). Cells were identified as regions of interest (ROIs ...
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bioRxiv - Immunology 2021Quote: ... 150 µl of enhancer 1 and 1.5 ml of enhancer 2 (Expifectamine transfection kit, Gibco) were added and cells cultured for an additional 3 days ...
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bioRxiv - Plant Biology 2022Quote: ... 2 ug of total RNA were treated with TURBO DNA-free Kit (Thermo Fisher Scientific) to remove any residual genomic DNA ...
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bioRxiv - Immunology 2023Quote: ... was mixed with 2 μL Buffer R for each reaction (Neon Transfection System Kit, Invitrogen), then mixed with 5 μL annealed crRNA:tracrRNA duplex ...
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bioRxiv - Pathology 2023Quote: ... and/or 2 mM SYTO RNA-Select Green Fluorescent Cell Stain Kit (Thermo Fisher Scientific) for 1 h at 37°C to stain RNAs inside EV ...
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bioRxiv - Genomics 2020Quote: Total RNA was extracted from whole blood using a PAXgene Blood RNA Kit version 2 (PreAnalytiX, #762164) and cDNA was generated with a High Capacity Reverse Transcription Kit (Applied Biosystems, #4368814) in an identical fashion as described for CDKL5 cDNA amplicon sequencing ...
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bioRxiv - Plant Biology 2020Quote: Edu (5-ethynyl-2′-deoxyuridine) staining was performed using Click-iT® EdU AlexaFluor® 647 Imaging Kit as per the kit protocol (Invitrogen). The 5 DAG Col-0 and flz8 seedlings grown on 0.5X MS medium with 30 mM Suc were fixed in the fixation reagent (1X PBS with 3.7% formaldehyde and 0.5% Triton-X ...
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bioRxiv - Pathology 2023Quote: ... were incubated with 20 μg/ml DQ gelatin fluorescein conjugate for 2 h at 37°C according to kit instructions (EnzChek Gelatinase/Collagenase Assay Kit, E-12055, Molecular Probes, Inc). The fluorescence generated by hydrolysis of the added substrate was captured by ZEISS Axioscan 7 (ZEISS Microscopy ...
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bioRxiv - Microbiology 2021Quote: ... The recommended quantity of purified nucleic acids were amplified using real time PCR kits for PCV-2 (LSI VetMAX™ Porcine Circovirus Type 2 Quantification, Thermo Fisher Scientific, MA, USA), Porcine reproductive and respiratory syndrome virus [PRRSV] European and North American strains (LSI VetMAX™ PRRSV EU/NA Real-Time PCR Kit ...
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bioRxiv - Physiology 2021Quote: ... mouse anti-mouse GAPDH (Thermo Fisher, #AM4300), rabbit-anti-mouse LSD1 (Abcam ...
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bioRxiv - Cancer Biology 2022Quote: The detection of TGF-β in the cell culture media from SH-SY5Y and MDA-MB-231 cells treated with TEPA for 24h was performed according to the protocol suggested by Thermo Fisher (TGF-beta-1-Human-ELISA-Kit/BMS249-4).
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bioRxiv - Developmental Biology 2020Quote: Total RNA was isolated from mouse embryos using the pico pure RNA isolation Kit (Thermo Fisher) and purified following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: The IFN-β levels in BALF were determined using mouse IFN beta ProQuantum Immunoassay kit (ThermoFisher). IFN-α and IFN-β levels in the supernatants of cultured BMDCs were determined by ELISA (PBL Biomedical Laboratories).
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bioRxiv - Cancer Biology 2021Quote: ... BV711 anti-mouse Tim3) and LIVE/DEAD fixable Aqua dead cell stain kit (ThermoFisher, Cat. # L34957) at room temperature for 15 min followed by flow cytometry.
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bioRxiv - Neuroscience 2020Quote: Mouse brain and lungs were processed to isolate total RNA using PureLink RNA Mini Kit (Invitrogen). An equal amount of total RNA was used to synthesize cDNA with a High-Capacity cDNA reverse transcription kit (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2021Quote: DNA was isolated from mouse saliva swabs using the PureLink™ Microbiome DNA Purification Kit (Invitrogen). ITS and FungiQuant qPCR primers [57 ...
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bioRxiv - Cell Biology 2020Quote: ... To generate mouse gapdh RNA probe the pTRI-GAPDH control construct (NorthernMax™-Gly kit, Ambion) was used as a DNA template for in vitro transcription ...
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bioRxiv - Cell Biology 2021Quote: LRRC23 was immunoprecipitated from mouse testis using the Pierce crosslink IP kit (Thermo Scientific, MA, USA) with anti-LRRC23 antibody described above ...
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bioRxiv - Neuroscience 2021Quote: ... OT-I CD8 T cells were isolated using a Dynabeads Untouched Mouse CD8 Cells Kit (Invitrogen). The purity of CD8 T cells was confirmed by flow cytometry (Fig S5) ...
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bioRxiv - Physiology 2024Quote: ... and γ subunits and mouse prostasin were transcribed using the mMessage mMachine transcription kit (Life Technologies). Resulting cRNA encoding each of the subunits was injected into stage V to VI Xenopus oocytes ...
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bioRxiv - Molecular Biology 2023Quote: ... AmpliSeq libraries were constructed using the Ion AmpliSeq Transcriptome Mouse Gene Expression Kit (Thermo Fisher Scientific) that is designed for targeted amplification of over 20,000 mouse RefSeq genes simultaneously in a single primer pool ...
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bioRxiv - Immunology 2022Quote: ... 0.5% 2-mercaptoethanol (2-ME) (GIBCO) and 1% HEPES (GIBCO) ...
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bioRxiv - Cancer Biology 2021Quote: ... primary antibodies were detected using Alexa Fluor 680 F(ab’)2 fragments of goat anti-mouse immunoglobulin G (IgG) or anti-rabbit IgG (Life Technologies) diluted at 1:8,000 in Blocking buffer ...
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bioRxiv - Neuroscience 2021Quote: ... into jaw closer muscles of 5-8 day old mouse pups with 1-2% dilution of Alexa Fluor 488 hydrazide (Life Technologies), under isoflurane anesthesia.
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bioRxiv - Cell Biology 2019Quote: ... 9101) were purchased from Cell Signalling. Mouse monoclonal anti tot-ERK1/2 (Cat. Nr. 13-6200) was purchased from Thermo Fisher. Mouse monoclonal anti-α-Tubulin (Cat ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed once and stained with goat anti-mouse IgG-Alexa Fluor 488 (2 μg/mL, # A11029, Thermo Fisher Scientific) for 30 min on ice ...
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bioRxiv - Immunology 2021Quote: ... The cells were washed and stained with 2 μg/mL goat anti-mouse IgG-Alexa Fluor 647 (# A21235, Thermo Fisher Scientific) in FACS buffer for 30 min ...
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bioRxiv - Immunology 2021Quote: ... at the concentration 1×106 cells per ml or Dynabeads Mouse T-Activator CD3/CD28 (ratio 1:2, Thermo Fisher Scientific) in the presence of protein transport inhibitor (BD GolgiStop™ ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was diluted with 400 μl of NET-2 buffer and antibody-conjugated Dynabeads M-280 Sheep anti-mouse IgG (ThermoFisher Scientific) beads were added ...
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bioRxiv - Molecular Biology 2022Quote: ... The membranes were incubated with horseradish Peroxidase-conjugated AffiniPure Goat Anti-Rabbit IgG and Goat Anti-Mouse IgG (ZSGB-BIO, Beijing, China) for 2 h and signals were observed using Super Signal West Pico (Thermo Scientific). Western blotting band intensity was quantified by densitometry analysis using ImageJ version 2x (NIH Image software ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were then washed 3x in PBS with 2% FBS and incubated with secondary antibody (Alexa Fluor 488-conjugated goat anti-mouse IgG) and fluorescently-tagged phalloidin (Molecular Probes), in a humidity chamber for 1hr ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated for 2 hours at room temperature in Alexa Fluor 488 donkey-anti-mouse (1:500 in TNB, Invitrogen, RRID:AB_141607). Following three washes with TNT ...
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bioRxiv - Molecular Biology 2019Quote: ... Membranes were washed with TBST and incubated for 1 h in TBST/2% BSA with secondary antibody (Immunopure goat anti-mouse # 31430, Thermo Scientific, and peroxidase-labeled affinity anti-rabbit # 04-15-06 ...
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bioRxiv - Pathology 2019Quote: ... Stimulation of T cells was achieved by adding 40 IU IL-2 and Dynabeads Mouse T-Activator CD3/CD28 (ThermoFisher Scientific) for 4 days ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were then washed and incubated at room temperature for 2 h in donkey anti-mouse alexa fluor 594 (1:1000, Life Technologies) and donkey anti-rabbit alexa fluor 488 (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Triton X-100 with 5 % normal-goat-serum and unconjugated F(ab′)2 goat anti-mouse IgG (H+L) antibody (1:500, Thermo Scientific), at 4 °C for 2 h ...