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Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for Mouse Anti Human Ig kappa chain Alexa Fluor 647 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... or anti-human CD68 (IgG3, kappa, PG-M1, ThermoFisher Scientific, MA, USA) antibodies were diluted 1:50 in PBS supplemented with 0.1% BSA and applied on the devices overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... plasmids of paired heavy chain (IgA1, IgA2) and kappa light chain together with a J chain were co-transfected into Expi293™ expression system (Thermo Fisher Scientific) at the ratio of 1:1:1 following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated with secondary antibodies (1:500 Goat anti -rabbit Alexa Fluor 647 [Thermo Fisher Scientific, A-21245], 1:500 Donkey anti-rat Alexa Fluor 594 [Thermo Fisher Scientific, A-21209]) in PBS for 2 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated with secondary antibodies (1:500 Goat anti -rabbit Alexa Fluor 647 [Thermo Fisher Scientific, A-21245], 1:500 Donkey anti-rat Alexa Fluor 594 [Thermo Fisher Scientific, A-21209]) in PBS for 3 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... or detected with a monoclonal mouse antibody (SantaCruz, sc-245, 1:250) and visualised with a donkey anti-mouse Alexa Fluor 647 secondary antibody (Molecular Probes, A31571, 1:1000). SCC1/ RAD21 was detected with a monoclonal mouse antibody (Millipore ...
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bioRxiv - Biophysics 2021Quote: ... The sections were rinsed in TBST and further incubated with the secondary antibody of goat anti-mouse FITC (1:500) or goat anti-rabbit Alexa Fluor-647(1:500) (Life Technologies, Thermo Scientific, USA) or goat anti-Guinea pig Alexa Fluor 555(1:500)for 2 h at room temperature in a humid chamber ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissues were washed with TBST followed by incubation with the secondary antibody such as anti-mouse FITC-488 (1:1000) or goat anti-rabbit Alexa Fluor-647 (Life Technologies, Thermo Scientific, USA) at room temperature for 2 h ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were washed 3 x 5 min with TBSB and incubated with conjugated anti-HRP (goat mAb, JacksonImmuno; 1:500) and secondary antibodies (Alexa Fluor 647 Donkey anti-mouse, Invitrogen at 1:500) for 2 hours at RT and washed with PBS ...
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bioRxiv - Cell Biology 2024Quote: Secondary antibodies used are: donkey anti-mouse or donkey anti-rabbit coupled to Alexa Fluor 594 or 647 (Invitrogen, ThermoFisher Scientific; 1:1,000). To amplify the GFP fluorescence intensity post-fixation ...
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bioRxiv - Cell Biology 2024Quote: Secondary antibodies used are: donkey anti-mouse or donkey anti-rabbit coupled to Alexa Fluor 594 or 647 (Invitrogen, ThermoFisher Scientific; 1:1,000). To amplify the GFP fluorescence intensity post-fixation ...
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bioRxiv - Microbiology 2020Quote: ... cells were then washed 3 times with PBS and incubated with goat anti-rabbit Alexa Fluor 488 or goat anti-human Alexa Fluor 488 secondary antibodies (Thermo Fisher Scientific) for 1h at RT ...
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bioRxiv - Cell Biology 2020Quote: Secondary antibodies were as follows: Alexa Fluor 488- or Alexa Fluor 594-conjugated to goat anti-mouse or goat anti-rabbit IgG (Invitrogen), Alexa Fluor 568-conjugated to goat anti-rat IgG (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were detected using 1 μg/ ml of anti-rabbit Alexa Fluor 594 or anti-mouse Alexa Fluor 488 in 1%BSA as appropriate (Invitrogen). All antibodies were incubated at room temperature for 1 hr ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by incubation with goat anti-rabbit IgG Alexa Fluor 488 and goat anti-mouse IgG Alexa Fluor 568 secondary antibodies (Invitrogen). Coverslips were mounted in Mowiol 4-88 (Calbiochem ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated with anti-mouse or anti-goat Alexa Fluor 488 and Alexa Fluor 594 antibodies (1:400; 1 hr at RT; Invitrogen) and counter-stained with DAPI nuclear dye (0.5 µg/ml in PBS ...
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bioRxiv - Microbiology 2022Quote: ... Bound primary antibodies were detected using 1:500-1:2000 dilutions of goat anti-mouse or goat anti-rabbit IgG secondary antibodies conjugated with Alexa Fluor 488 or Alexa Fluor 594 (Invitrogen). Tachyzoite and HFF nuclei were labelled with DAPI (4’,6’-diamidino-2-phenylindole) ...
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bioRxiv - Physiology 2022Quote: ... Specific staining was revealed using anti-mouse or anti-rabbit Alexa Fluor 488 and Alexa Fluor 555-conjugated secondary antibodies (InVitrogen) with Hoechst33358 nuclear stain (0,1 μg/ml ...
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bioRxiv - Genetics 2022Quote: ... Secondary antibodies goat anti-mouse Alexa Fluor 488 (1:200) and goat anti-rabbit Alexa Fluor 568 from Life Technologies were used for visualization ...
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bioRxiv - Immunology 2020Quote: ... Coverslips were then washed 3 times with PBS-saponin and stained with anti-mouse Alexa Fluor 488 and anti-rabbit Alexa Fluor 568-conjugated secondary antibodies (Invitrogen), and Hoechst for 1 hour at RT ...
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bioRxiv - Immunology 2021Quote: ... The cells were then washed 3x PBS and incubated with species-specific Alexa Fluor 488 conjugated goat anti-rabbit or Alexa Fluor 594 conjugated goat anti-mouse secondary antibodies (1:300; Invitrogen). The cells were then washed 3x PBS and mounted on a 1.5 mm coverslip with a Prolong™ Gold Antifade Mountant with DAPI.
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bioRxiv - Biochemistry 2020Quote: ... Alexa Fluor 488 Goat anti-mouse antibody and Alexa Fluor 568 Goat anti-rabbit antibody were obtained from Life Technologies and used at 1:2000 for immunofluorescence.
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were then stained with secondary antibodies: Alexa Fluor 488 anti-mouse or Alexa Fluor 488 anti-rabbit (ThermoFisher Scientific) for 1.5 hours at room temperature and washed in the same manner ...
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bioRxiv - Cell Biology 2022Quote: ... the tissues were stained with 1:2000 goat anti-mouse Alexa Fluor 594 and goat anti-rabbit Alexa Fluor 488 secondary antibodies (Invitrogen) at 25°C for 2 hours ...
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bioRxiv - Cancer Biology 2019Quote: ... Coverslips were then washed three times in PBS before incubation with secondary antibodies (Alexa Fluor 555 goat anti-mouse and Alexa Fluor 555 goat anti-rabbit; Invitrogen) at room temperature for 1 hour in a humidified chamber ...
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bioRxiv - Microbiology 2021Quote: ... This was followed by 3×washes with PBS then incubation with Alexa Fluor 488 goat anti-mouse or Alexa Fluor 594 goat anti-rat antibodies (1:1000; Invitrogen) in PBS containing 3% BSA with DAPI ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were washed three times with PBS and incubated with the corresponding secondary antibodies (1:1000; anti-rabbit Alexa Fluor 488, Invitrogen, A11008; anti-mouse Alexa Fluor 488, Invitrogen, A11001 ...
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bioRxiv - Cell Biology 2020Quote: ... Specific staining was revealed using anti-mouse or anti-rabbit Alexa Fluor 488 and Alexa Fluor 555-conjugated secondary antibodies (InVitrogen) with Hoechst33358 nuclear stain (0.1 µg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... The recommended concentration of secondary antibodies (Goat Anti-Rabbit Alexa Fluor 488, A-11034 and Goat Anti-Mouse Alexa Fluor 568, A-11031, Invitrogen) were added and the sections were incubated for 1 hour ...
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bioRxiv - Microbiology 2019Quote: ... Secondary antibodies included goat anti-mouse or anti rabbit IgG conjugated to Alexa Fluor 488 or Alexa Fluor 568 (Invitrogen) for immunostaining ...
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bioRxiv - Microbiology 2019Quote: ... followed by goat anti-mouse IgG Alexa fluor-594 conjugate and goat anti-rabbit IgG Alexa fluor-488 conjugate (Invitrogen). Images were captured using an DeltaVision OMX SR imaging system (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... Hoechst 33258 and secondary antibodies goat anti-mouse IgG Alexa Fluor 488 (A11001) and goat anti-rabbit IgG Alexa Fluor 555 (A21428) were obtained from Invitrogen.
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bioRxiv - Biochemistry 2022Quote: ... and subsequently probed with alexa fluor 488-conjugated anti-His or anti-mouse IgG (H + L) Cross-Adsorbed Secondary Antibody Alexa Fluor 568 (ThermoFisher), respectively ...
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Synthetic lethal targeting of TET2-mutant hematopoietic stem and progenitor cells by XPO1 inhibitorsbioRxiv - Cancer Biology 2022Quote: ... were co-incubated at 4°C overnight and visualized with Alexa Fluor 488 goat anti-mouse secondary antibody and Alexa Fluor 546 goat anti-rabbit secondary antibody (Invitrogen). TUNEL apoptosis assay was performed on embryos with the In-Situ Cell Death Detection kit (POD ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies (anti-rabbit IgG-Alexa Fluor 633 and anti-mouse IgG-Alexa Fluor 488) were purchased from Thermo Fisher Scientific and used at 1/1000 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated with secondary antibodies (1:500; Alexa Fluor goat anti-rabbit 488, A11008; Alexa Fluor goat anti-mouse 594, A11032; Invitrogen), and mounted with DAPI Fluoromount-G ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were incubated in anti-mouse Alexa Fluor 488 and anti-rabbit Alexa Fluor 594 (Thermo Fisher Scientific, Waltham, MA) diluted in 1× TBS with 1% BSA for 1 hour at RT in a dark room ...
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bioRxiv - Neuroscience 2024Quote: ... the bound antibodies were detected with an Alexa Fluor 488 goat anti-rabbit IgG or Alexa Fluor 568 goat anti-mouse IgG (Invitrogen), and the nuclei were labeled with DAPI in HBSS containing 5% BSA and 1% normal donkey serum solution for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... for 20 min at RT and incubated with 3% NGS PBS containing secondary antibodies (1:600, Alexa-Fluor 594 anti-mouse IgG, Invitrogen and Alexa-Fluor 488 anti-guinea pig IgG, Invitrogen) for 90 min at RT ...
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bioRxiv - Microbiology 2024Quote: ... The secondary antibodies were applied O/N on a shaker: Alexa Fluor 594 anti-mouse IgG (H+L) and Alexa Fluor 488 anti-rabbit IgG (H+L) (both Invitrogen), both at 1:500 dilutions in 2% BSA/PBS ...
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bioRxiv - Microbiology 2024Quote: ... Secondary antibodies included Alexa Fluor 594- or Alexa Fluor 488-conjugated goat anti-rabbit-IgG and goat anti-mouse-IgG (Invitrogen), all used at 1:1000.
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Sections were incubated in secondary antibodies (1:1000 Alexa Fluor 488 anti-rabbit IgG [Invitrogen]; 1:1000 Alexa Fluor 488 anti-mouse IgG [Invitrogen] ...
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bioRxiv - Plant Biology 2023Quote: ... the samples were incubated with 1:200 diluted Alexa Fluor 488-conjugated goat anti-mouse antibody and Alexa Fluor 594-conjugated goat anti-rabbit antibody (Invitrogen) for 1 h ...
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bioRxiv - Genomics 2023Quote: ... Antibodies for immunofluorescence staining were goat anti-mouse Alexa Fluor 488 (A11029, 1:300) and goat anti-rabbit Alexa Fluor 555 (A21428, 1:300) from Invitrogen.
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were washed three times in antibody dilution buffer and then incubated with an appropriate secondary antibody in antibody dilution buffer (1:500 dilution; Alexa Fluor 488-conjugated goat anti-rabbit IgG or Alexa Fluor 594-conjugated goat anti-mouse IgG; Invitrogen) at room temperature for 1 h ...
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bioRxiv - Immunology 2024Quote: ... Secondary antibodies (Donkey anti-mouse Alexa Fluor 594, 1: 200, and Donkey anti-rabbit Alexa Fluor 488; 1:100; both from Invitrogen) were applied for 1 hour at room temperature ...
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bioRxiv - Physiology 2024Quote: ... was acquired from Novas Biologicals and Anti-Rabbit IgG Alexa fluor 555 (#A32732) and Anti-Mouse IgG Alexa fluor 488 (#A32723) were procured from Invitrogen. Primary antibodies SQSTM1/p62 (D1Q5S ...
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bioRxiv - Cancer Biology 2021Quote: ... and Alexa Fluor 647 conjugated Goat anti-Rabbit IgG (1:1000; Invitrogen™ A27040). After staining ...
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bioRxiv - Cell Biology 2020Quote: ... anti-rat Alexa Fluor 647 from goat (A21247, Invitrogen, at 1:1000 for IF), peroxidase-conjugated anti-mouse from goat (115-035-003 ...
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bioRxiv - Cell Biology 2020Quote: ... Goat Anti-Chicken Alexa Fluor® 647 (4 μg/ml, A-21449, Molecular Probes), Goat Anti-Mouse Alexa Fluor® 488 antibody (8 μg/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... slides were incubated with anti-rabbit Alexa Fluor 647 (1:500, Fisher Scientific; A21244), anti-mouse or anti-goat Cy3 (1:250 ...