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Citations for Thermo Fisher :
2151 - 2200 of 3328 citations for HVEM Rhesus Macaque HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... YP_009724390.1) ectodomain was produced in 500mL cultures of HEK293F cells grown in suspension using FreeStyle 293 expression medium (Life technologies) at 37°C in a humidified 8% CO2 incubator rotating at 130 r.p.m ...
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bioRxiv - Physiology 2020Quote: ... stable inducible cell lines expressing WT or mutant EAAT2 were generated by selection of Flp-In T-Rex 293 cells (Invitrogen) transfected with pcDNA5/FRT/TO-EAAT2 and used either with or without induction with tetracycline (38,50,52).
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bioRxiv - Microbiology 2021Quote: ... Transfections were carried out using 1 mg of total DNA (500 μg each of VH and Vκ plasmid DNA) and 1 l of cultured HEK293-F cell suspension maintained in sterile Freestyle 293 expression medium (Invitrogen) without antibiotics at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... Cells grown to a density of 3 million cells per mL were transfected using pCMV::SARS-CoV-2_S_NTD derivative mutants with the ExpiFectamine™ 293 Transfection Kit (ThermoFisher Scientific) with and cultivated for five days at which point the supernatant was harvested ...
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bioRxiv - Immunology 2019Quote: ... 1 μg/mL puromycin and expanded after adapting cells in suspension to Expi-293 medium (Life Technologies, Grand Island, NY) supplement with 1 μg/mL puromycin ...
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bioRxiv - Biochemistry 2021Quote: Human embryonic kidney 393T cells were adapted to suspension culture in Gibco Freestyle 293 medium (Thermo Fisher Scientific, Waltham USA) supplemented with 20 U/L penicillin ...
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bioRxiv - Biochemistry 2021Quote: Human embryonic kidney cells adapted to suspension culture (HEK293F) were grown in Free Style 293 Expression Medium (Thermo Fisher Scientific) and harvested at a density of 3 million cells/ml ...
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bioRxiv - Immunology 2020Quote: ... were transfected with the plasmid expressing S-2P protein ectodomains by ExpiFectamine 293 transfection kit and ExpiFectamine CHO transfection kit (ThermoFisher), respectively ...
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bioRxiv - Immunology 2021Quote: ... Cells grown to a density of 2.5 million cells per mL were transfected using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) and cultivated for four days at which point the supernatant was harvested ...
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bioRxiv - Microbiology 2020Quote: ... and HEK293S GnTI-/- cells (ATCC CRL-3022) in Freestyle 293 Expression Medium supplemented with 2% ultra-low IgG FBS (Gibco). We maintained ...
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bioRxiv - Biochemistry 2022Quote: ... RBD of SARS-CoV2 and SARS-CoV S were transiently expressed in FreeStyle™ 293-F cells (Thermo Fisher Scientific). Cells were resuspended at 1×106 per mL with fresh media on the day of transfection ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids encoding the heavy chain and light chain of antibodies were transfected into Expi293F cells using an Expifectamine 293 transfection kit (Gibco) in a 2:1 mass ratio following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... CaVβ3-stable and HEK293S GnTI-cells were grown in suspension at 37°C supplied with 8% CO2 in FreeStyle 293 Expression Medium (Gibco) supplemented with 2% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... sequence confirmed plasmid DNA was diluted in OptiMEM I serum-free medium and incubated for 20 minutes with ExpiFectamine 293 Reagent (Gibco). The DNA– ExpiFectamine 293 Reagent complexes were used to transfect Expi293F cell cultures in 96-deep-well blocks ...
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bioRxiv - Immunology 2022Quote: All SARS-CoV-2 S ectodomains were produced in 500-mL cultures of FreeStyle™ 293-F cells (ThermoFisher Scientific) grown in suspension using FreeStyle 293 expression medium (ThermoFisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... 293 cells (106 cells) expressing CD177 were treated by 0.2 unit PI-PLC (cat# P-6466, ThermoFisher Scientific, Waltham, Massachusetts) in 0.5 ml PBS for 1 h at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... and RSV B/ 18537 DS-Cav1 (51) constructs were expressed by transient transfection of FreeStyle 293-F cells (Thermo Fisher) with polyethylenimine (PEI ...
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bioRxiv - Cell Biology 2023Quote: ... Adeno-X 293 cells (Takarabio Cat #632271) were transfected with linearized adenoviral vectors using Lipofectamine and Plus Reagent (Thermo Fisher). Cells were dislodged with gentle agitation upon exhibiting late cytopathic effect and were lysed with three freeze-thaw cycles ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein was produced from IL-2 and IL-12 expressing stable lines during one-week culture in serum-free media (Freestyle 293, Invitrogen) and purified with HisPur Ni-NTA affinity resin (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... Flp-In T-REx 293 cells were grown in DMEM supplemented with 10% (v/v) fetal bovine serum (FBS; Invitrogen), 100µg/mL zeocin (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: Plasmids encoding the heavy chain and light chain of the antibody were transfected at an equimolar ratio into Expi293F cells using ExpiFectamine 293 transfection kit (Gibco) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... The full-length spike proteins were stabilized by introducing 2 prolines at amino acid positions 986 and 987 (Spike 2P) and transiently transfected in FreeStyle 293-F cells using Turbo293 (SpeedBiosystems) or 293Fectin (ThermoFisher). The constructs contained an HRV3C-cleavage site followed by His and/or streptagII tags for purification purposes ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with pcDNA4TO HMM-HA-Spike and pcDNA tagBFP for transfection marker using ExpiFectamine 293 (Thermo Fisher Scientific). Cells were centrifuged at 200 g for 2 min 48 h after transfection and washed with Dulbecco’s phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2023Quote: ... with a secretion sequence and C-terminal His6-tag) were transiently expressed using FreeStyle™ 293-F cells (Thermo Fisher) in FreeStyle™ F17 Expression Medium supplemented with L-glutamine and 1X MEM non-essential amino acids (Gibco) ...
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bioRxiv - Molecular Biology 2023Quote: ... human kidney) were maintained at 37°C and 9% CO2 in a 75% humidified atmosphere in FreeStyle 293 Expression Medium (GIBCO). The Plasmid was transiently transfected using Turbo293 (SpeedBiosystems ...
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bioRxiv - Cell Biology 2023Quote: ... Y-A or YIY-AAA) and FLAG-tagged CDK-1 constructs were expressed in FreeStyle 293-F cells (ThermoFisher Scientific). Cells were transfected using FreeStyle MAX Reagent and OptiPRO SFM according to the manufacturer’s guidelines (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... were transfected with expression vectors for 3xFLAG-tagged wt eIF3g or its mutated forms using Expifectamine 293 according to the manufacturer’s protocol (Thermo Scientific). Cells expressing 3xFLAG-tagged eIF3g (200 ml suspension ...
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bioRxiv - Microbiology 2023Quote: ... suspension-adapted and serum-free HEK293S cells were seeded at 3 × 106 cells/mL in Freestyle-293 medium (ThermoFisher Scientific). Next ...
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bioRxiv - Immunology 2023Quote: ... plasmids encoding for various SCTs were co-transfected with a plasmid encoding for an ER-retained BirA-ligase into FreeStyle 293-F cells (Invitrogen) using the 293-free transfection reagent (Merck ...
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bioRxiv - Biochemistry 2023Quote: The WDR76 knockout cell line was derived from Flp-In™-293 Cell Line purchased from Invitrogen (Carlsbad, CA, USA). The knockout was achieved using RNA-guided CRISPR (Clustered regularly interspaced short palindromic repeats)-Cas9 system (51) ...
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bioRxiv - Immunology 2023Quote: The cells used for expression of the rIgE clones construct were Freestyle 293-F cells (Thermofisher, cat no: K900001/510029). A 1 mL stock of 1*10^7 cells were rapidly thawed from −80°C in 37°C water bath and transferred to 19 mL prewarmed Freestyle™ 293 Expression Medium (Thermofisher ...
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bioRxiv - Biochemistry 2022Quote: HEK293F cells were cultured in serum-free FreeStyle 293 Expression media using 125 mL disposable culture flasks (Thermo Fisher Scientific). Cells were shaken constantly at 125 rpm at 37°C with 5% CO2 ...
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bioRxiv - Biophysics 2022Quote: ... CaVβ3-stable cells were grown in suspension at 37°C supplied with 8% CO2 in FreeStyle 293 Expression Medium (Gibco) supplemented with 2% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2022Quote: Murine neuroblastoma Neuro-2a cells (#CCL-131; ATCC, Manassas, VA, USA) and Flp-In T-REx 293 cells (Thermo Fisher) were maintained in DMEM (D5796 ...
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bioRxiv - Neuroscience 2023Quote: ... 7 x 106 AAV-293 cells were seeded into 150 mm cell culture plates in DMEM (# 41965062, Thermo Fisher Scientific) containing 10% fetal bovine serum (#16000-044 ...
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bioRxiv - Biophysics 2023Quote: ... HEK293S GnTI- suspension cells (ATCC CRL-3022, lot number 62430067) were cultured at 37 °C in Freestyle 293 medium (Gibco) supplemented with 2% (v/v ...
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bioRxiv - Biochemistry 2023Quote: Expression and purification of secreted SIDT1 and SIDT2 extracellular domain The SIDT1ECD and SIDT2ECD proteins were expressed using FreeStyle 293-F cells (Invitrogen). The cells were cultured in OPM-293 CD05 medium (OPM Biosciences co. ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids encoding the heavy chain and light chain of antibodies were transfected into Expi293F cells using an Expifectamine 293 transfection kit (Gibco) in a 2:1 mass ratio following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... EXPI293 cells were diluted to 0.5×105 cells per millilitre 48 hours prior to transfection with FreeStyle 293 Expression Medium (ThermoFisher Scientific). Cells counts were adjusted to 1.5×106 cells/mL immediately prior to transfection ...
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bioRxiv - Biochemistry 2024Quote: ... Stable cell lines for expression were generated using the doxycycline-inducible Flp-In T-Rex 293 system (Thermo Fisher Scientific) according to the manufacturer’s guidelines.
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bioRxiv - Immunology 2024Quote: ... Plasmids encoding both the heavy chain and light chain for each antibody were co-transfected into FreeStyle 293-F cells (Invitrogen) using polyethylenimine ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids encoding heavy and light chains were transfected into Expi293F cells in a 2:1 mass ratio using an ExpiFectamine 293 transfection kit (Gibco). Six days post-transfection ...
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bioRxiv - Microbiology 2024Quote: ... with a plasmid encoding for a human furin ectodomain (residues 1–794) into 40 mLs of FreeStyle 293-F cells (ThermoFisher). Cells were transfected at a density of 1 million cells/mL using 25 kDa linear polyethylenimine (PEI) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... alecto EFNB2 and EFNB3 were expressed in Expi293F cells by transient transfection using the ExpiFectamine 293 Transfection Kit (Thermo Fisher) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2024Quote: ... and human embryonic kidney cell line HEK293 Flp-In T-Rex cells (Cellosaurus Flp-In-T-REx-293 (CVCL_U427)) were maintained in Dulbecco’s Modified Eagle Medium (DMEM) (GibcoTM, Thermo Fisher Scientific) including 10% FBS ...
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bioRxiv - Biophysics 2024Quote: ... HEK-293 cells were grown to 40%-50% confluency in DMEM (GenDEPOT) supplemented with 10% FBS (GenDEPOT) and penicillin/streptomycin (Invitrogen) and transfected with GluA2(Q) ...
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bioRxiv - Immunology 2024Quote: ... Plasmids encoding antibody heavy and light chain genes were used to transfect suspension Expi293F cells in a 1:2 heavy to light chain ratio using ExpiFectamine 293 transfection reagents (Gibco) according to the manufacturer’s instructions ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... ExpiCHO cells were transiently transfected with the vector containing the gene encoding soluble spike glycoprotein ectodomain or CR3022 (using a heavy chain:light chain ratio of 1:2) using the Expifectamine 293 Transfection Kit (ThermoFisher #A29129), following the user manual ...
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bioRxiv - Microbiology 2024Quote: ... including a C-terminal His6-tag and transfected into mycoplasma screened Expi293F™ GnTI- cells using ExpiFectamine 293 (ThermoFisher Scientific). Mutagenesis of RBK21 constructs was carried out using the Q5 Site Directed Mutagenesis kit (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... 2ug of CBP-pRetroX-pTRE3G constructs or pRetroX-Tet3G were co-transfected with 2ug of the envelope pVSV-G plasmid into GP2-293 cells using lipofectamine 3000 (ThermoFisher). After 48 hours ...