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Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for Cleaved PARP Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... and further digested for 5 min at room temperature (22°C) using a TrypLE Express recombinant protease (ThermoFisher Scientific, cat. #12605010). The dissociated cells were sedimented by centrifugation (900 g for 10 min at 4°C) ...
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bioRxiv - Molecular Biology 2021Quote: ... designed using CRISPOR) and 7.5 μg recombinant Cas9 protein (Vienna Biocenter Core Facilities) using the Neon Transfection System (Thermo Fisher Scientific). A week later ...
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bioRxiv - Microbiology 2019Quote: ... The cell lysate was incubated with superparamagnetic beads covalently linked to recombinant protein G (Dynabeads protein G Novex, Life technologies, USA) to obtain the precleared lysate ...
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bioRxiv - Developmental Biology 2019Quote: ... Recombinant HSF2 (TP310751 Origen) was desalted (ZebaTM Spin Desalting Columns, 7K molecular-weight cutoff, 0.5 ml (1034–1164, Fisher Scientific, Germany)) and biotinylated at a molar ratio biotin/protein (3:1 ...
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bioRxiv - Cell Biology 2019Quote: ... sequence-verified plasmids (wild-type and mutant variants of pbiG2ab:6His-ZZ-SNAPf-DHC1/IC2/LIC2/Tctex1/Robl1/LC8) were used to generate recombinant baculoviral genomes by Tn7 transposition into DH10Bac cells (Life Technologies). White ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μg of purified GST-MKLP1620-858 proteins (wild type and AQA mutant) bound to beads were phosphorylated in vitro with 2 μg of recombinant His-tagged Aurora B (Thermo Fisher) as described10,27 and then washed three times with 500 μl of phosphatase buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Developmental Biology 2019Quote: ... was introduced into the BglII and SpeI sites of pCR4-HBB-24XMS2SL and the recombinant HBB- 24XMS2SL-MCS sequence was subcloned into the pcDNA3 mammalian expression vector (ThermoFisher Scientific) using the NotI and XbaI sites ...
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bioRxiv - Biochemistry 2021Quote: Recombinant baculoviruses for expression of the mTTYH3 ECD1-T4L-ECD2 construct were generated using the Bac-to-Bac system (ThermoFisher Scientific). Sf9 cells were cultured at 27 °C in 1 L culture flasks with ESF-921 insect cell culture medium (Expression systems) ...
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bioRxiv - Cancer Biology 2020Quote: ... The phage library was incubated with biotinylated CD40-Fc fusion protein or Her2 recombinant protein (Acro biosystems) for 2 hours at RT and the phage-antigen complex was captured by Dynabeads M280 (Life technologies). The bound phages were eluted by Glycin-HCl (pH 2.2 ...
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bioRxiv - Immunology 2021Quote: CASP1-/- and NLRP1-/- N/TERT-1 keratinocyte cells were prepared by delivery of Cas9 ribonucleoprotein complexes containing an Alt-R CRISPR-Cas9 sgRNA and recombinant Cas9 (IDT) using the Neon Transfection System (ThermoFisher Scientific) following the manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2020Quote: ... luminescence was compared to a ladder of recombinant Gaussia Luciferase (NanoLight #321-500) quantified using a Gaussia Luciferase Glow Assay Kit (ThermoFisher #16161) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Anthony White (QIMR Berghofer Medical Research Institute, Australia) were maintained on human recombinant vitronectin in StemFlex medium (Life Technologies, cat. # A3349401). Differentiation was performed as previously described [15 ...
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bioRxiv - Cancer Biology 2020Quote: ... and 150 ng were used for the BP recombinant reaction with pDONR221 according to the Gateway technology manual (Thermo Fisher Scientific). Chemically competent E ...
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bioRxiv - Cell Biology 2021Quote: ... two and three consecutive phage enrichment rounds were performed either with recombinant Miro1 immobilized on NuncTM ImmunoTM MaxiSorpTM tubes (Thermo Scientific) or HEK293-expressed GFP-Miro1 immobilized on ChromoTek GFP- Trap® Multiwell plate (Proteintech) ...
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bioRxiv - Microbiology 2020Quote: One 96-well plate was coated with 200ng/well of recombinant N protein and another plate was coated with 200ng/well of recombinant 6xHis-tagged SARS-CoV-2 spike protein receptor binding domain (RBD) produced using the FreeStyle 293 Expression System (Thermo Fisher) from BEI Resources (Cat# NR-52366 ...
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bioRxiv - Systems Biology 2020Quote: ... 72 h after seeding on 96-well microplates differentiated cells were incubated with recombinant human interferon gamma (IFN-γ, ThermoFisher, #PHC4031) at concentrations 0-10 ng/mL or recombinant human interleukin 10 (IL-10 ...
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bioRxiv - Plant Biology 2021Quote: ... Inserts from the recombinant pENTR plasmids were transferred to destination vectors via gateway cloning by using the LR clonaseTM II Enzyme mix (Invitrogen, USA) and by following the manufactureŕs instructions ...
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bioRxiv - Genomics 2021Quote: Cryopreserved stocks of the KH004 parent and the Mal31×KH004 uncloned bulk recombinant progeny pool and were thawed and grown in complete media (CM) [RPMI 1640 with L-glutamine (Gibco, Life Technologies), 50 mg/L hypoxanthine (Calbiochem ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was added to 15 mL of glutathione agarose resin (which binds up to 40 mg of purified recombinant GST protein per milliliter of resin) (Thermo Scientific) and incubated on ice (4°C ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant baculovirus was generated in Sf9 cells using the Bac-to-Bac system according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Recombinant proteins were then expressed by infecting Hi5 cells for 96 h at 28°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Canada and maintained in keratinocyte serum-free media supplemented with 0.05 mg/ml of bovine pituitary extract and 0.02 μg/ml EGF recombinant human protein (Life Technologies, Benicia, CA) in a 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... or HIF1β were expressed in Sf9 cells that had been infected with recombinant baculovirus as described in the Bac-to-Bac® Baculovirus Expression System from Invitrogen.
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bioRxiv - Cell Biology 2021Quote: ... The His-tagged recombinant fusion protein was separated by cobalt immobilized metal chelate affinity beads using the HisPur Cobalt Purification Kit (Thermo Scientific) following the procedure described by the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: ... coated overnight with 10 μg/ml of xeno-free human recombinant Laminin 521 (LN521, Biolamina) prepared in GMP DPBS+/+ (A1285801, Life Technologies) by reversing the strainer and washing the EBs into the plate with GMP N2 media (CTS™ DMEM-F12 ...
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bioRxiv - Molecular Biology 2022Quote: ... intracellular ATP levels were determined by reaction of ATP with recombinant firefly luciferase and its substrate D-luciferin according to the manufacturer’s instructions (Thermo Scientific, A22066).
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bioRxiv - Immunology 2022Quote: ... Cells were incubated in RP-10 supplemented with 10 ng/ml recombinant human IL-2 and 2 μg/ml anti CD28 (clone 37.51, Thermo Fisher Scientific) for 18-22 hours ...
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bioRxiv - Physiology 2022Quote: ... Success of the transformation and choice of the recombinant plasmids was verified in LB agar cultures (Lennox L Agar, Thermo Fisher) containing Ampicillin (200 mg/mL ...
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bioRxiv - Microbiology 2020Quote: ... recombinant SARS-CoV-2 spike protein was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10% fetal bovine serum (FBS)) and 30IU/mL recombinant human IL-2 (rhIL-2; Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Immunology 2022Quote: The α−TD monoclonal antibody was produced in Sf9 insect cells by using a Bac-to-Bac recombinant baculovirus system (Invitrogen). The monoclonal antibody manufacturing process started with the revival ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting plasmids were utilized for the generation of recombinant Baculoviruses according to the Bac-to-Bac expression system protocol (Invitrogen #10359016). Exponentially growing Spodoptera frugiperda 9 cells (2E06 cells/mL in Lonza Insect-XPRESS medium #BELN12-730Q ...
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bioRxiv - Plant Biology 2020Quote: ... cDNAs from pUC57 entry vector were transformed by electroporation into Escherichia coli strain DH10B and transferred by a recombinant LR reaction of Gateway cloning (Clonase II enzyme mix Kit, Thermo Fisher) into pB7WGF2 (Karimi et al. ...
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bioRxiv - Microbiology 2020Quote: All recombinant DNA manipulation was conducted according to standard procedures [18] and the recommendations of the enzyme manufacturer (Thermo Scientific, USA). Plasmid and chromosomal DNAs were isolated with Plasmid Miniprep (Evrogen ...
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bioRxiv - Cell Biology 2020Quote: ... HUVECs were treated with 30 ng/ml of VEGF (Recombinant Human Vascular Endothelial Cell Growth Factor, Fisher Scientific 10690920, Bordeaux, France) for 24 hours.
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bioRxiv - Microbiology 2021Quote: ... PCR was performed using 50 ng of the template and the PCR mix contained 1U of recombinant Taq DNA Polymerase (Invitrogen™), 1x buffer ...
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bioRxiv - Biochemistry 2021Quote: The pFAST-bac1 vectors were used to produce recombinant baculovirus according to the protocol for the Bac-to-Bac system (Life Technologies), except using Insect GeneJuice (EMD Millipore ...
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bioRxiv - Pathology 2021Quote: ... His-tag recombinant SARS-CoV-2 Spike Protein S1/S2 (S-ECD) (1 ng/ml; ThermoFisher Scientific; aa11-1208; RP-87680) or Flag-tag recombinant SARS-CoV-2 Spike RBD (1 ng/ml ...
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bioRxiv - Cell Biology 2020Quote: Recombinant 6XHis-tagged TbVps24 (N-terminus tag) was cloned using the pTrcHis TOPO TA expression system (Thermo Fisher Scientific, Carlsbad, CA) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Monoclonal antibodies or recombinant SARS-CoV-2 RBD + SBD1 or NTD proteins were transfected in FreeStyle 293F suspension cells (Life Technologies) using PEIMAX transfection reagent (Polysciences) ...
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bioRxiv - Immunology 2021Quote: Thermal melt analysis of the recombinant proteins was performed in triplicate in 96-well plates in a QuantStudio 7 Flex real time PCR machine (Applied Biosystems). Each well contained 2-4 μg protein in buffer (25mM TRIS pH 8 ...
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bioRxiv - Molecular Biology 2020Quote: ... # 236-EG), 20 ng/ml recombinant human FGF basic protein (R&D, # 4114-TC),1% penicillin/streptomycin (P/S, Invitrogen, SV30010), 1% sodium pyruvate (Gibco ...
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bioRxiv - Immunology 2020Quote: ... CD8α+IEL were expanded in complete medium containing 50 ng/ml recombinant mouse IL-15/IL-15R complex (Thermo Fisher Scientific) on a tissue culture plate at the concentration of 5×105 cells/ml for 3 days ...
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bioRxiv - Immunology 2021Quote: Purified CD8+ cells were cultured in AIMV medium supplemented with 10% FBS and 100 U/ml recombinant human IL-2 (ThermoFisher Scientific) and used for sorting of CD8+ T cell subsets and for further experiments three days after isolation.
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bioRxiv - Immunology 2022Quote: ... Recombinant MARV-Angola GPdTM (IBT Bioservices) was tethered to Fluospheres NutrAvidin-Microspheres yellow-green or red (Thermo Fisher Scientific, Waltham, MA) using the EZ-link Micro Sulfo-NHS-LC-Biotinylation kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2022Quote: ... Organoids were cultured in basement membrane extract (BME, Trevingen) and overlaid with growth medium consisting of murine recombinant epidermal growth factor (EGF 50 ng/ml, Life Technologies), murine recombinant Noggin (100 ng/ml ...
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bioRxiv - Cell Biology 2022Quote: ... 50 mM β- Mercaptoethanol and 1 ng/ml human recombinant leukemia inhibitory factor) for 24 h and then treated with 0.1 μg/ml Colcemid (Gibco, 15210-040) for 2.5 h ...
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bioRxiv - Genetics 2022Quote: ... Secondary antibodies: Goat anti-Mouse IgG (H+L) Superclonal Recombinant Secondary Antibody Alexa Fluor 555 (1:200, ThermoFisher Scientific catalogue #A28180), Goat Anti-Rabbit IgG H&L Alexa Fluor 488 (1:200 ...
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bioRxiv - Microbiology 2022Quote: ... The CHO cell growth and Spike recombinant plasmid transfection were performed according to the ExpiCHO expression system User Guide (MAN0014337, ThermoFisher website). The S-trimer expression was under the control of a strong CMV promoter ...
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bioRxiv - Molecular Biology 2022Quote: ... using conditions recommended by the supplier (1× PNK buffer without ATP, 2 U/μl RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific), incubation at 37°C for 2 h) ...
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bioRxiv - Molecular Biology 2022Quote: ... generally according to the suppliers’ recommendations (1× EPAP buffer, 1 mM ATP, 1 mM DTT, and 0.8 U/μl RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific), incubation at 37°C for 1 h) ...