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Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The culture medium was refreshed every day and cells were passaged every 4–5 days using 1 mg/mL Dispase II (Thermo Fisher Scientific). Cells were routinely tested for mycoplasma contamination ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were rinsed 3x 10 min with PBS and incubated either for 2 hrs at RT or 24-72 hrs at 4° C in goat anti-rabbit Alexa Fluor 488 (1:1000, Invitrogen, ThermoFisher Scientific) secondary antibody in carrier solution containing 1% NGS ...
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bioRxiv - Cell Biology 2023Quote: ... cells at 70-80% confluency were switched to myogenic differentiation medium (DMEM, 4% horse serum, 2 mM Glutamax, 1 mM sodium pyruvate (Life Technologies #11360070), 1 μg/mL insulin (GeminiBio #800122 ...
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bioRxiv - Cell Biology 2024Quote: ... They were then incubated overnight at 4 °C with the primary antibody (anti-TMEM173/STING antibody (OT14H1),1:100 dilution) (Invitrogen, MA,USA). After they were rinsed with PBS-T ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were passaged with a ratio of 1:4 to when they reached 70% to 80% confluency by incubation cells with 0.5 mM EDTA (Fisher Scientific MT-46034CI) at room temperature for 5 minutes.
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bioRxiv - Neuroscience 2023Quote: ... for 1 h at 4°C before the addition of 25 μL of Pierce Protein A/G Magnetic Beads (Thermo Fisher Scientific) for an additional 2 h under constant rotation ...
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bioRxiv - Bioengineering 2023Quote: ... in PBS pH 7.4 or pH 6), HA (Sigma-Aldrich, 9067-32-7, 0.5%–1% w/v in Hanks’ Balanced Salt Solution (Gibco, 14025-050) or Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were co-transfected at ∼80% confluence with wild-type (4 μg) or mutant SCN1A construct (4 μg) and enhanced green fluorescent protein (eGFP (1 μg) using Lipofectamine 3000 Reagent (Thermo Fisher Scientific) and incubated under the culture conditions above for 24 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed 4-5 times in PBS for 2 hours then incubated for 3-4 hours with secondary antibody (anti-rabbit Alexa Fluor 568; 1:1000; Invitrogen; Cat #A10042) and NeuroTrace Green Fluorescent Nissl Stain (1:2000 ...
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bioRxiv - Microbiology 2023Quote: The RBDs of G614, Alpha, Beta, and Omicron (BA.1, BA.2, BA.4/5) variants were ordered as GeneString from GeneArt (Thermo Fisher Scientific). All sequences of the RBD (aa 319-541 in GenBank ...
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bioRxiv - Cancer Biology 2022Quote: Approximately 5 × 106 MCF10AER/vSrc cells (Control and treated with 10 nM D-Alanine for 4 h) were fixed with 1% methanol-free formaldehyde solution (Thermo Scientific, #28908) for 10 min at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were pre-extracted with 0.1% Tween-20 in PBS (PBST) for 1 min and then fixed with 4% methanol-free formaldehyde (Thermo Fisher Scientific, 28906) for 20 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... hTERT immortalized BJ-5ta foreskin fibroblasts were cultured in a 4:1 mixture of DMEM medium and Medium 199 (Gibco, Life Technologies) supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2023Quote: ... 3 µL of 1 mM Fluo-4 AM (stock solution in DMSO) and 30 µL of PowerLoad Concentrate (100X, Thermo Fisher Scientific) were vortexed vigorously ...
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bioRxiv - Developmental Biology 2023Quote: ... the sections were incubated overnight at 4°C with the following primary antibodies diluted in blocking buffer: rat anti-SOX2 (1:200, 14-9811-82, Thermo Fisher Scientific), goat anti-T (1:200 ...
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bioRxiv - Physiology 2023Quote: ... 100 ng pMD2 and 400 ng of each sgRNA CRISPR Cas9 lentivirus plasmid (plasmid amount rate 3:1:4) using Turbofect transfection reagent (Thermo Fisher Scientific). Lentiviral media was centrifuged once at 1500 x g for 3 minutes and the supernatant was collected ...
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bioRxiv - Biochemistry 2023Quote: ... then resuspended in 100 µL of secondary antibody (1:500 α-mouse IgG-Alexa647 [ThermoFisher, Waltham, MA] in TBST with 4% BSA), and incubated in the dark for 60 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... incubated at 65°C for 5 min and then immediately chilled on ice for at least 2 min before adding 4 μL reverse transcription reaction mix [in 10 μL RT reaction: 1× First Strand buffer (Invitrogen – 18064- 014), 20 mM DTT (Invitrogen - 18064-014) ...
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TNFR1/p38αMAPK signaling in Nex+ supraspinal neurons regulates sex-specific chronic neuropathic painbioRxiv - Neuroscience 2023Quote: ... for two hours at room temperature Sections were incubated in the following primary antibodies overnight at 4 °C: ERβ (rabbit, 1:200, Invitrogen PA1-311) and NeuN (mouse ...
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bioRxiv - Genomics 2023Quote: ... for 1 hour, followed by incubation with primary antibodies (overnight, 4°C) and secondary antibodies (1-2 hours, from Jackson ImmunoResearch or ThermoFisher Scientific). The slides were then mounted with coverslips using VECTASHIELD (Vector Labs ...
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bioRxiv - Immunology 2023Quote: ... CTLA-4 or PD-1) were suspended in IL-2 medium at 37°C containing either 65nM LysoTracker® Deep Red (ThermoFisher Scientific) or 25nM MitoTracker™ Red CMXRos for 1h or 30min ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then stained with primary antibodies overnight at 4°C targeting the following proteins: MAP2 (PA1-10005, ThermoFisher Scientific, 1:5000), AT8 (MN1020 ...
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bioRxiv - Cell Biology 2023Quote: ... Ex vivo lung slices were washed 3 × 30 minutes in PBS+0.5% Triton X-100) before incubating overnight at 4°C overnight with: 1:100 Alexa Fluor 488 goat anti-rabbit (Thermo Scientific - A11008) or anti-mouse (A32723 ...
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bioRxiv - Biochemistry 2023Quote: ... ATP measurements were taken before and after activation with magnetic Dynabeads coated with anti-CD3/CD28 antibodies (Thermofisher, 4:1 beads/cell).
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bioRxiv - Cell Biology 2023Quote: ... primary antibodies in 10% GS in 1xPBS overnight at 4°C followed by a 1h incubation with anti-mouse (A11003, Invitrogen, 1:800) secondary antibody in 10% GS in 1xPBS at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... The lysate was cleared at 45’000⍰g for 25⍰min at 4⍰°C and the supernatant was loaded into HisPur NiNTA Chromatography 1 ml cartridge (Thermo Fisher Scientific). The resin was washed with 50 ml of buffer A containing 20⍰mM imidazole ...
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bioRxiv - Developmental Biology 2023Quote: ... mucus-like compounds were stained by incubation (overnight at 4°C) with AlexaFluor 647-labeled PNA (1:1000 in PBSt; Molecular Probes #L32460).
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bioRxiv - Neuroscience 2023Quote: ... secondary antibodies were incubated on slides in a the dark for 3 hours at room temperature or overnight at 4°C: GαM 488 (Invitrogen A21121, 1:1000), GαRb 546 (Invitrogen A11010 ...
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bioRxiv - Immunology 2023Quote: ... a liver burn injury of approximately 1 mm3 was made on which a droplet of pHrodo Red succinimidyl ester (4 μM; Thermo Fisher Scientific) was administered ...
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bioRxiv - Immunology 2023Quote: ... The sections were fixed for 1 hour with 4% paraformaldehyde (PFA) in Hank’s Balanced Salt Solution (HBSS, Gibco, Waltham, MA, pH 7.2) supplemented with 0.1% Bovine Serum Albumin (BSA ...
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bioRxiv - Cell Biology 2023Quote: ... the coverslips were washed and fixed with 4% paraformaldehyde in PBS for 10 min at room temperature then stained with Alexa Fluor 488 Phalloidin (1:500; Invitrogen cat# A12379) in blocking buffer for 20 min at room temperature and mounted in DuoLink PLA mounting medium with DAPI ...
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bioRxiv - Cell Biology 2023Quote: ... were plated into 12-well plate at a density of 3-4 × 105 cells in 1 mL per well in 1x DMEM (Gibco Cat# 11995065) plus 10% FBS (Gibco Cat# 16000044 ...
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bioRxiv - Molecular Biology 2023Quote: ... lysates were incubated for overnight at 4°C with 2 μg of antibodies recognizing MTR4 or IgG control and then incubated for 1 h at 4°C with Dynabeads™ Protein A (ThermoFisher Scientific). After incubation ...
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bioRxiv - Physiology 2023Quote: ... and incubated for a period of 1 h in a dark room in 2 ml of extracellular solution containing 5 µM fluo- 4 AM (Thermo Fisher Scientific) or 7 µM Corona Green AM (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... with a FuGene: DNA ratio of 3:1 using 4 μg of DNA in OptiMEM Reduced Serum Medium (ThermoFisher Scientific Catalog #11058021). The transfection medium was removed from cells ∼6–8 h post transfection ...
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bioRxiv - Neuroscience 2023Quote: ... a series of every 6th section was immersed in 1% skim milk for 1 h at room temperature and incubated overnight at 4°C with rabbit anti-GFP monoclonal antibody (1:500, G10362, Thermo Fisher Scientific) i n PBS containing 0.1% Triton X-100 and 1% normal goat serum for 2 days at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed in PBS three times for 5 min at 4 °C by rocking and then incubated by rocking with secondary antibody (Goat-anti-Mouse 488, 1:2000, Invitrogen #A-11008) in 1% BSA PBST for 1 hour at RT in the dark ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were blocked with 5% non-fat dry milk overnight at 4°C under gentle agitation followed by 1,5 hours incubation at room temperature with anti-CRM1 (MA5-27879, Invitrogen, 1:2000 dilution) antibody to detect PmXPO1 protein ...
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bioRxiv - Cell Biology 2023Quote: ... 5 µl polar metabolite extract were injected in push partial mode using an overfill factor of 1 onto a Dionex IonPac AS11-HC column (2 mm × 250 mm, 4 μm particle size, Thermo Fisher Scientific), additionally this was equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm ...
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bioRxiv - Biochemistry 2023Quote: ... The grid was blotted for 3s (blot force 1) at 100 % humidity and 4°C in a Vitrobot Mark IV (Thermo Fisher Scientific) before plunge-freezing in liquid ethane.
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bioRxiv - Genomics 2023Quote: ... 5 ul of cDNA reaction was used in each of 4 x 50 ul Round 1 PCRs per sample using Phusion polymerase in HF buffer (Life Technologies-F531L). The 4 Round 1 PCRs were then pooled and 5 ul of that mix were used as template for a 50 ul Round 2 PCR ...
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bioRxiv - Cancer Biology 2024Quote: ... De-crosslinking was conducted using 4 µl of 5 M NaCl and 1 µl of 20 mg/ml proteinase K (Thermo Fisher Scientific) for 4 h at 60 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... 200 ng of RNA were mixed with 4 µl of diluted ERCC RNA Spike-In Mix (Invitrogen, cat# 4456740. 1:1,000 dilution). All libraries were sequenced on the Illumina NovaSeq 6000 system.
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bioRxiv - Bioengineering 2024Quote: ... Then secondary antibodies were then added in blocking buffer over night at 4 ºC (Goat anti Rabbit 647, Thermo Fisher Scientific A21245, 1:1000; Rhodamine Phalloidin, Fisher Scientific 50-646-256 ...
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bioRxiv - Genomics 2024Quote: ... The membrane was blocked with 5% non-fat milk for 1 hour at RT and incubated overnight at 4°C with primary antibody [Sfrp1 (Invitrogen, MA5-38193), Nr3c1 (Cell signaling ...
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bioRxiv - Cancer Biology 2024Quote: ... The PVDF membrane was blocked for 1 h with 5% BSA in TBST buffer containing 0.1% Tween 20 followed by overnight incubation at 4 °C with primary antibody for: ERα (MA5-14104, dilution 1:400, Thermo Fisher Scientific) and PR (sc-7208 ...
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bioRxiv - Immunology 2024Quote: CD14-positive monocytes isolated from human PBMCs (1 x 106 cells/well) were incubated with 27D6 antibody or isotype-matched control antibody (4 μg/mL, IgM, Thermo Fisher Scientific) for 30 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... The membrane was blocked with 5% non-fat milk for 3 hours and incubated overnight at 4°C with primary antibodies against COL1 (1:1000, Invitrogen, PA5-95137), COL3 (1:1000 ...
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bioRxiv - Immunology 2024Quote: ... Program T-023 with 150 pmol/106 cells of human TSP-1 and TSP-4-specific siRNAs (#s14108 and #s14100, Thermo Fisher Scientific) and control scrambled siRNAs (#4390846 ...
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bioRxiv - Cell Biology 2024Quote: ... Intracellular 7-ketocholesterol was measured by fixing cells in 4% paraformaldehyde and staining with 7-ketocholesterol monoclonal antibody at 1:50 (3F7, Invitrogen, MA5-27561) diluted in 1X permeabilization buffer (Invitrogen ...