Labshake search
Citations for Thermo Fisher :
2101 - 2150 of 10000+ citations for OF 1 CAS 919973 83 4 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... and incubated overnight at 4°C with anti-ChAT (choline acetyltransferase) goat polyclonal antibody (1:500, Invitrogen #PIPA518518) and anti-c-Fos rabbit polyclonal antibody (1:1000 ...
-
bioRxiv - Microbiology 2023Quote: ... supplemented with 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and 10% Hi-FBS (Thermo Fisher Scientific). BSC-1 cells (ATTC ...
-
bioRxiv - Microbiology 2023Quote: ... DNA concentration was measured with 1×dsDNA HS Assay Kit on a Qubit 4 Fluorometer (Thermo Fisher Scientific). The four plate pools were pooled equimolarly to a final dilution of 2 nM and paired-end sequenced (2 × 250 ...
-
bioRxiv - Immunology 2022Quote: ... stimulator PBMCs were irradiated at 35 Gy and cultured (ratio 4:1) with CFSE-labeled responder PBMCs (Invitrogen, Waltham ...
-
bioRxiv - Cell Biology 2022Quote: ... Nuclei were counterstained using 4′,6-diamidin-2-phenylindol (DAPI, 1:1000, 7 min; D1306, Thermo Fisher Scientific). Washing steps were performed with PBS ...
-
bioRxiv - Bioengineering 2022Quote: ... the cell nuclei were stained with 1 μg/mL 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific #62248) in DPBS for 15 min at room temperature ...
-
bioRxiv - Developmental Biology 2022Quote: ... and pGL4.74-Renilla luciferase plasmids in a ratio of 0.5:1:0.1 (4 μg/6-well dishes) using Lipofectamine LTX PLUS (Invitrogen). The luciferase activity was assayed using the Dual-Luciferase Reporter Assay Kit (Promega) ...
-
bioRxiv - Neuroscience 2023Quote: ... Slices were either washed using DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride; 1:10000; Thermo Fisher Scientific, MA) made up in di H2O to stain for nuclei before being mounted or cover-slipped with ProLong Gold mounting medium with DAPI (Fisher Scientific ...
-
bioRxiv - Biophysics 2023Quote: ... in 3% BSA with a 1:50 ratio and 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) were employed ...
-
bioRxiv - Cell Biology 2023Quote: ... supplemented with 4 mM L-alanyl-L-glutamine and NucBlue nucleus staining reagent (1 drop per mL) (Invitrogen). Plates were incubated for 15 min (37 °C ...
-
bioRxiv - Cell Biology 2023Quote: ... supplemented with 4 mM L-alanyl-L-glutamine and NucBlue Live ReadyProbes Reagent (1 drop per mL) (Invitrogen). Plates were incubated for an additional 10 min (37 °C ...
-
bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were stained overnight in 4 °C with rat-anti-CD4 (1:100, Invitrogen, 14-9766-82) and rabbit-anti-Iba1 (1:500 ...
-
bioRxiv - Microbiology 2024Quote: ... the grid was blotted for 4 sec with a force of 1 using a Vitrobot Mark IV (ThermoFisher) and plunge-frozen into liquid ethane ...
-
bioRxiv - Microbiology 2024Quote: ... the cell nucleus was stained with 1:1000 DAPI (4’,6’-diamidino-2-phenylindole; Invitrogen; Catalog No: D1306) nuclear stain for 10 minutes at RT ...
-
bioRxiv - Molecular Biology 2024Quote: ... cDNA was diluted 1:4 and 2 μL was used in a 20 μL qPCR Taqman assay (Invitrogen). Assay probes were used to detect cDNA from Nkx2.9 (Mm00435145_m1) ...
-
bioRxiv - Neuroscience 2024Quote: iPSC neurons were loaded with calcium-sensitive fluorescent Fluo-4 dye (1×, Direct Calcium Assay Kit, Invitrogen, USA) in humidified incubator (5% CO2 ...
-
bioRxiv - Neuroscience 2024Quote: ... cells were then incubated overnight at 4°C with a P2RY12 primary antibody (1:100, 702516, Thermo Fisher) or TMEM119 primary antibody (1:50 ...
-
bioRxiv - Molecular Biology 2024Quote: ... as published.62 Nuclei was visualized by 1 μg/ml 4′,6-diamidino-2-phenylindole (DAPI, from ThermoFisher) as published.23
-
Placentas from SARS-CoV-2 infection during pregnancy exhibit foci of oxidative stress and DNA damagebioRxiv - Microbiology 2024Quote: ... Sections were incubated overnight at 4℃ with primary antibodies specific for SARS-CoV-2 Nucleocapsid (1:300, Invitrogen), Spike (1:500 ...
-
bioRxiv - Microbiology 2024Quote: ... Cells were then fixed in 4% paraformaldehyde and stained with BODIPY™ 493/503 (Invitrogen, 1 μg/ml) in 150 mM sodium chloride ...
-
bioRxiv - Biochemistry 2024Quote: ... GMPCPP-stabilized microtubule seeds were prepared by polymerizing a 1:4 molar ratio of tetramethylrhodamine (TAMRA, ThermoFisher Scientific) labeled:unlabeled tubulin (Hyman et al. ...
-
bioRxiv - Physiology 2024Quote: ... to identify cytoplasmic F-actin fragments and 4’,6-diamidino-2-phenylidole (DAPI) (Invitrogen Cat. #D1306; 1:1,000) to label nuclei ...
-
bioRxiv - Molecular Biology 2024Quote: ... Mitochondrial proteins were solubilized with 1% digitonin resolved on 4-16% or 3-12% NativePAGE Novex gels (Invitrogen). For blotting ...
-
bioRxiv - Molecular Biology 2024Quote: ... overnight at 4°C and incubated in secondary antibody (1:500 DyLight 488 goat anti-mouse, Invitrogen 35502) for 1 h at room temperature ...
-
bioRxiv - Biochemistry 2024Quote: ... The peptides were then labelled with tandem mass tag (TMT) reagent (1:4; peptide:TMT label) (Thermo Fisher Scientific). After quenching ...
-
bioRxiv - Neuroscience 2024Quote: ... slices were incubated in secondary antibody (Table 2) with 4’,6-diamidino-2-phenylindolefor (DAPI, 1:1000, Invitrogen) 45 minutes at room temperature in a rocking platform ...
-
bioRxiv - Developmental Biology 2024Quote: ... we homogenized the tissues in 1 ml TRIzol solution using the Bead Mill 4 homogenizer (Thermo Fisher Scientific). Following phase separation with 500 µl chloroform ...
-
bioRxiv - Cancer Biology 2024Quote: ... a 4 min incubation in secondary antibodies Mouse Alexa Fluor Plus 555 (1:200, A32727, Thermo-Fisher Scientific) Rabbit Alexa Fluor Plus 647 (1:400 ...
-
bioRxiv - Biochemistry 2024Quote: ... the mixture was ultracentrifuged at 1,77,500g and 4°C for 1 hour to remove insoluble debris using T-647.5 rotor (Thermo Fisher) and Sorvall WX 100 Ultra Series Centrifuge ...
-
bioRxiv - Cancer Biology 2024Quote: ... cell nuclei were stained with 1 µg 4’,6-Diamidino-2-Phenylindole (DAPI, Invitrogen, Thermo Fisher Scientific, D1306) for 15 min in a humidified incubator at 37 °C and 5% CO2 ...
-
bioRxiv - Cell Biology 2024Quote: ... and 4 dpf were injected with 1–2 nL of dextran tetramethyl rhodamine (molecular weight 2000 kDa, Invitrogen) at a concentration of 10 mg/mL ...
-
bioRxiv - Cancer Biology 2024Quote: ... cell nuclei were stained with 1 µg 4’,6-Diamidino-2-Phenylindole (DAPI, Invitrogen, Thermo Fisher Scientific, D1306) for 15 min in a humidified incubator at 37 °C and 5% CO2 ...
-
bioRxiv - Developmental Biology 2024Quote: ... They were then stained overnight at 4°C in a 1:100 dilution of Alexa647-conjugated phalloidin (ThermoFisher) in PBS ...
-
bioRxiv - Developmental Biology 2021Quote: Fibroblasts were cultured in Dulbecco’s Modified Eagle’s medium (DMEM) containing 1 g/L glucose supplemented with 10% heat-inactivated fetal bovine serum (FBS) and 1% antibiotic-antimycotic (Life Technologies, Carlsbad, CA, USA).
-
bioRxiv - Neuroscience 2021Quote: ... The sections were then incubated for 48h at 4°C in the same solution with mouse anti-NeuN (1:2000; Chemicon; MAB377, Temecula, CA, USA) and anti-human PHF-Tau antibody clone AT8 (1:2000, MN1020, Thermo Scientific, Waltham, MA, USA); for the sake of clarity ...
-
bioRxiv - Molecular Biology 2022Quote: ... TGF-β1 and monocyte chemotactic protein-1 (MCP-1) was measured using the ELISA kit following the manufacturer’s protocol (eBioscience Affymetrix, Santa Clara,CA, United States). The levels of Muc5ac and Muc5b in BALF were also measured according to Lu et al ...
-
bioRxiv - Neuroscience 2020Quote: ... A 10 µL reaction scale TaqMan method qPCR for Gapdh was performed using 1 µL of 1:40 diluted 20 cycle PCR products with 7900HT Fast Real-Time PCR System (Applied Biosystems, Foster City, CA) or CFX384 Touch Real-Time PCR Detection System (Biorad ...
-
bioRxiv - Immunology 2020Quote: ... Cells were washed 4X in PBST and subsequently incubated with secondary antibodies (goat anti-rabbit IgG 488, Invitrogen, Carlsbad, CA; #A-11034, 1:4000; & goat anti-human 647, Invitrogen #A-21445, 1:4000) for 1 hour at room temperature ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections then transferred to species-specific secondary antibodies conjugated with either AlexaFluor 594 (1:200) or AlexaFluor488 (1:100, Invitrogen-Molecular Probes, Carlsbad, CA) for 1.5 hours in room temperature ...
-
bioRxiv - Genomics 2021Quote: ... overnight at 4° C followed by goat anti-rabbit Alexa fluor 594 secondary antibody (1:1000 in PBS with 1% (w/v) BSA) (Invitrogen, Carlsbad, CA, cat. A1102) incubation for 1 hr at room temperature ...
-
bioRxiv - Neuroscience 2022Quote: ... and then stained in a PBS solution containing 1% skim milk and avidin conjugated to Alexafluor 594 (1:200, Life Technologies, Carlsbad, CA, USA) for 2 hrs ...
-
bioRxiv - Neuroscience 2024Quote: ... the sections were incubated 1 hour in secondary antibodies: goat anti-rabbit or goat anti-mouse AlexaFluor488/546 (1:1000; Invitrogen Life Technologies, Carlsbad, CA). Sections were mounted (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2023Quote: ... mouse monoclonal anti-rat DC217 (1:200, Axon Neuroscience R&D SE, Bratislava, Slovakia) and polyclonal anti-rat pThr212 (1:1000, Invitrogen Life Technologies, Carlsbad, CA). For total tau ...
-
bioRxiv - Cancer Biology 2022Quote: ... followed by incubation with secondary antibodies Alexa Fluor 488 goat anti-rabbit diluted 1:200 and Alexa Fluor 555 goat anti-mouse diluted 1:200 (Life Technologies, Carlsbad, CA, USA) and counterstaining with DAPI ...
-
bioRxiv - Microbiology 2020Quote: ... 4 μL of AlamarBlue (Invitrogen) was added and plates were incubated in a Biotek HT plate reader at 37 °C for 4 h ...
-
bioRxiv - Developmental Biology 2021Quote: ... and 4% FBS (ThermoFisher; 10082147) in PBS ...
-
bioRxiv - Biophysics 2022Quote: ... 4 mM L-Glutamine (Gibco), in the presence of penicillin-streptomycin (Gibco ...
-
bioRxiv - Immunology 2022Quote: ... and Qubit 4 Fluorometer (Invitrogen).
-
bioRxiv - Cell Biology 2022Quote: ... in 4 ml OptiPro (Invitrogen). The mixture was incubated for 20 min at room temperature before being added to the cell cultures ...
-
bioRxiv - Neuroscience 2021Quote: ... 0.1 fluo-4 (Molecular Probes), 0.1 Alexa Fluor 568 (for morphological visualization ...