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Citations for Thermo Fisher :
2101 - 2150 of 8443 citations for IL 12RB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... T cell activation was assessed using mouse IL-2 ELISA kits (Invitrogen), with samples diluted 1:2 −1:20 for comparison to the standard curve ...
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bioRxiv - Immunology 2020Quote: ... The following ELISA kits were used: IL-1β Mouse ELISA kit (ThermoFisher), IL-18 Mouse ELISA kit (ThermoFisher) ...
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bioRxiv - Biochemistry 2021Quote: ... PCR amplification was performed using TaqMan® master (Thermo Scientific, Rockford, IL) with the Roche LightCycler 480 Real-Time PCR system (AB7900HT) ...
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bioRxiv - Bioengineering 2021Quote: ... Detection was performed using an enhanced chemiluminescent substrate (Thermo Scientific, Rockford, IL). All primary antibodies against phosphorylated proteins were obtained from Cell Signaling Technologies (Beverly ...
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bioRxiv - Immunology 2022Quote: ... + 200 IU/mL IL-2 (NCI) + 0.08 mM 2-Mercaptoethanol (Gibco, 21985023) (NK-92 Media ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were developed with SuperSignal West Dura Substrate (Thermo Scientific, Rockford, IL) and imaged with a Bio-Rad Chemidoc MP imager (Bio-Rad).
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bioRxiv - Biochemistry 2019Quote: ... Alexa Fluor 594-conjugated secondary antibody (1:2000) (Invitrogen, Rockford, IL, USA) was added onto cells ...
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bioRxiv - Zoology 2020Quote: ... RPMI-1640 medium was sourced from Gibco (Gibco, Thermo Scientific, IL, USA). T4 DNA Ligase and JetFlex™ Genomic DNA Purification Kit was obtained from Invitrogen (Thermo Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... IL-7 20ng/ml (Miltenyi) and Lipids (hLDL) 50ng/ml (Life Technologies)63.
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bioRxiv - Cell Biology 2019Quote: ... siRNF167_2 separately using RNAimax (13778-075, Thermo Fisher Scientific, Rockford, IL, USA) in reduced serum-containing media (Opti-MEM ...
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bioRxiv - Cell Biology 2020Quote: ... antibodies to COPII (Sec23) and HS3ST5 from Thermo Scientific (Rockford, IL, USA) and antibody to HS2ST (N-term ...
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bioRxiv - Plant Biology 2019Quote: ... Biotinylated proteins were detected with streptavidin-HRP (21124, Thermo Scientific, Rockford, IL).
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bioRxiv - Plant Biology 2019Quote: ... Biotinylated protein was detected with streptavidin-HRP (21124, Thermo Scientific, Rockford, IL).
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bioRxiv - Molecular Biology 2020Quote: ... determined using the Pierce™ BCA Protein Assay Kit (Thermo Scientific, IL).
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bioRxiv - Neuroscience 2019Quote: ... and monoclonal rat anti-mouse IL-6 (1:50; Invitrogen, MP5-20F3) were diluted in 3% normal donkey serum and incubated overnight at 4°C ...
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bioRxiv - Pathology 2021Quote: ... Knockdown experiments were performed using Silencer Select siRNA from Ambion STAT3 (4392420) and IL-6RAa (4390771) transfected using Lipofectamine RNAiMax Reagent (Invitrogen). All cells tested negative for mycoplasma contamination using MycoAlert (Lonza ...
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bioRxiv - Cell Biology 2020Quote: ... and reverse transcribed to cDNA using SuperScript VILO MasterMix (ThermoFisher, Rockford, IL). Samples were then subjected to qPCR using PowerUp SYBR Green Master Mix (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: ... RNA was extracted with TRIzol™ Reagent (ThermoFisher Scientific, Rockford IL, USA) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... filled with isopropanol (423830025, Acros Organics, Fisher Scientific, Hanover Park, IL, USA). Cells were then stored at -140 °C in liquid nitrogen (CryoPlus Storage Systems model 7402 ...
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bioRxiv - Immunology 2020Quote: ... and α-IL-2 (JES6-5H4, 25-7021-80, Thermo Fisher Scientific) for 2 hours on ice ...
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bioRxiv - Developmental Biology 2020Quote: ... and goat anti-rabbit Alexa-Fluor 488 or 546 (Invitrogen, Rockford, IL) were used as secondary antibodies at a concentration of 1:300 diluted in blocking solution for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 ng/ml IL-4 (Thermo Fisher Scientific, Cat#14-8041-62) and 1 ng/ml TGF-β1 (Cell Signaling Technology ...
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bioRxiv - Plant Biology 2022Quote: ... and three protease inhibitors: 1 mM AEBSF (ThermoFisher, Scientific, Rockford, IL, USA), 10 mM 1-10 phenanthroline (Sigma Aldrich Chimie SARL ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with protease inhibitor cocktail kit (Thermo Fisher Scientific, Rockford, IL, USA). Cell lysates were incubated overnight with 90 μL of Dynabeads® MyOne™ Streptavidin C1 (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... IL-2 APC (MQ1-17H12, eBioscience) and IFN-g-PE (B27; Invitrogen). Other antibodies used were anti-murine CD19-eFluor450 (1D3 ...
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bioRxiv - Microbiology 2024Quote: ... the primers were treated with T4 Polynucleotide Kinase (Thermo Scientific, Rockford, IL) for 5’ phosphorylation according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... PBS and ELISA kits (TNFa and IL-6) were purchased from Invitrogen. MTT (M2128 ...
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bioRxiv - Genetics 2024Quote: ... mouse anti-mHsp70 (1:600; Thermo Scientific, Rockford, IL; Cat: MA3–028), rabbit anti-COUP-TF1 [1:1,000 (IB) ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 100 IU/ml IL-2 (Thermo Fisher Scientific Waltham, MA), 5% Human AB Serum (Valley Biomedical ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 mM tris(2-carboxyethyl)phosphine (TCEP) (Thermo Scientific, Rockford, IL, USA), 20 mM chloroacetamide (CAA) ...
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bioRxiv - Cell Biology 2023Quote: ... IL-8 and TNF-α were measured using ELISA kits from Invitrogen (Catalog# BMS224-2 ...
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bioRxiv - Immunology 2023Quote: ... 400fmol of Pierce® Retention Time Calibration Mixture (Thermo Scientific, Rockford, IL) was spiked into samples to track retention times and mass drift across all samples ...
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bioRxiv - Cancer Biology 2023Quote: ... and phophopeptides were enriched using titanium dioxide spin tips (Thermo Fisher, IL). LC-MS/MS analysis was performed on an Orbitrap ProVelos Elite MS system (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Samples were desalted using Pierce C18 Tips (ThermoFisher Scientific, Rockford, IL, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... According to the Micro BCA assay (Thermo Fisher Scientific Inc, Rockford, IL), every sample was normalized to 4 mg/mL and 200 μL diluted in 5% BSA in TBST to 1 mL ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentrations were then estimated by BCA assay (ThermoFisher Pierce, Rockford, IL). Disulfide bonds were reduced by incubation with 5 mM DTT (45 min ...
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bioRxiv - Microbiology 2023Quote: ... 0.95 µg of MS grade trypsin (Thermo Scientific Pierce, Rockford, IL, USA) in 40 µl of ABC was added to filters and incubated for 16 hours in a wet chamber at 37 °C ...
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Activation of glucocorticoid receptor signaling inhibits KSHV-induced inflammation and tumorigenesisbioRxiv - Cancer Biology 2023Quote: ... ELISA was performed using the rat IL-1α ELISA Kit (Invitrogen, BMS627) and IL-1Ra ELISA Kit (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Ultrapure formic acid (FA) was purchased from Thermo Scientific (Rockford, IL, USA).
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bioRxiv - Molecular Biology 2024Quote: ... and regularly checked for IL-3 addiction by performing 3x PBS (Gibco) washes and outgrowth in the absence of IL-3 to confirm cell death in the parental ...
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bioRxiv - Microbiology 2024Quote: ... The cytokine IL-1β was measured by ELISA (Invitrogen, #88-7013A-88) according to manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2020Quote: ... using Taqman probes (human RBM10: Assay ID, Hs00275935_m1, human Bcl-xL: Assay ID, Hs00236329_m1, Applied Biosystems, human Bcl-xS ...
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bioRxiv - Biochemistry 2022Quote: ... Human serum (catalog # 4522 from human male AB plasma) and fetal bovine serum were from Gibco, Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... and plasma human glucagon was measured by the human glucagon ELISA kit (Thermo Scientific, Frederick, MD). Exendin-4 plasma levels were measured using the exendin-4 EIA kit (Phoenix pharmaceuticals ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 50 μL fresh FACS buffer and 10 μL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Immunology 2022Quote: ... Total IgG in human sera was measured using a commercial human IgG ELISA kit (Fisher Scientific) according to manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... All human CD8+ T cells were activated with anti-human CD3/CD28 dynabeads (11131D, Thermo Scientific) at a 1:1 cell-to-bead ratio and cultured in complete RPMI supplemented with 10% FBS ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 50 µL fresh FACS buffer and 10 µL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 50 µL fresh FACS buffer and 10 µL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Neuroscience 2023Quote: ... Secreted Aβ42 peptides were quantified using the Human Amyloid β1-42 human ELISA kit (Thermo Fisher) according to the manufacturer’s protocol ...