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Citations for Thermo Fisher :
2101 - 2150 of 5122 citations for Cytomegalovirus Purified Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The mRNA was then further purified using the Dynabeads™ mRNA Purification Kit (ThermoFisher). The m6A levels in mRNA were detected by colorimetric ELISA assay with the EpiQuikTM m6A RNA Methylation Quantification kit (Epigentek ...
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bioRxiv - Biochemistry 2022Quote: ... All proteins were initially purified using HisPur Ni-NTA affinity resin (Thermo Fisher Scientific) using Ni-NTA buffer supplemented with 25 mM imidazole during wash and 250 mM imidazole during elution ...
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bioRxiv - Cell Biology 2022Quote: ... Purified proteins were analysed by SDS-PAGE and Instant Blue (10616474, Expedeon, ThermoFisher Scientific) staining ...
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bioRxiv - Biochemistry 2022Quote: 5 μM purified PWWP domain was incubated with 5× SYPRO Orange (Thermo Fisher Scientific) in assay buffer (20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: The supernatants were further purified using Pierce peptide desalting spin columns (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... gRNAs were in vitro transcribed and purified using the MAXIscript T7 kit (ThermoFisher # AM1312), according to the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: Purified naïve splenic B cells were stained with 5 μM CellTrace™ Violet (Invitrogen) in PBS for 20 minutes at room temperature in the dark ...
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bioRxiv - Immunology 2022Quote: ... RNA from virus stocks was purified using TRIzol Reagent (ThermoFisher Scientific, catalog no. 15596026). Brief ...
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bioRxiv - Microbiology 2022Quote: All PCR products were purified using the ExoSAP-IT PCR Product Cleanup (ThermoFisher Scientific) or Zymoclean Gel DNA Recovery Kits (ZYMO Research ...
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bioRxiv - Genomics 2022Quote: ... The 15N-GST-H4 peptide fusion was purified on glutathione agarose resin (Thermofisher Scientific), cleaved with PreScission Protease (16 hr incubation at 4°C) ...
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bioRxiv - Molecular Biology 2022Quote: ... and column purified RNA was biotinylated using Pierce RNA 3’ End Biotinylation Kit (ThermoFisher). Recombinant His-tagged NSP12 (R&D Systems ...
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bioRxiv - Molecular Biology 2022Quote: ... In vitro transcription was performed on the purified templates using the MegaShortScript kit (ThermoFisher), and column purified RNA was biotinylated using Pierce RNA 3’ End Biotinylation Kit (ThermoFisher) ...
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bioRxiv - Immunology 2022Quote: ... The composition of the purified antibodies was verified by NuPAGE gradient SDS-PAGE (ThermoFisher) in both reducing and non-reducing conditions plus Krypton fluorescent whole protein staining (ThermoFisher) ...
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bioRxiv - Microbiology 2022Quote: ... The PCR product was purified using E-GelTM SizeSelectTM II 2% agarose gel (Invitrogen). RNA-seq library for 300bp insert size was constructed following the manufacturer’s instructions (NEBNext UltraTM II DNA Library Prep Kit) ...
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bioRxiv - Genetics 2022Quote: ... PCR products were then purified and ligated into a pCRTM4-TOPO® vector (Invitrogen). StellarTM cells (Takara ...
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bioRxiv - Genetics 2022Quote: ... The fragments were gel purified and ligated using T4 DNA ligase (15224-017 Invitrogen life technologies ...
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bioRxiv - Immunology 2022Quote: ... The plasmid was purified using PureLink™ HiPure Plasmid Maxiprep Kit (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2022Quote: 5 μg of purified protein was mixed with 5× SYPRO orange (Thermo Fisher Scientific) in 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Then the purified PCR products were ligated into the pJet2.1 vector (Thermo Fisher Scientific) and transformed into the E ...
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bioRxiv - Neuroscience 2022Quote: ... The PCR products were purified using ExoSAP-IT PCR product Cleanup (Thermo Fisher Scientific) and analyzed by direct sequencing using the ABI PRISM 3730xl Genetic Analyzer ...
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bioRxiv - Bioengineering 2022Quote: Expi293F cells were transfected with the purified plasmids using ExpiFectamine 293 transfection kit (Invitrogen), where the fusion proteins were expressed and harvested at 96 hrs post-transfection under 8% CO2 at 37°C ...
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bioRxiv - Immunology 2022Quote: ... mRNA was purified from total RNA (5µg) using Dynabeads Oligo (dT)(Thermo Fisher Scientific) with two rounds of purification ...
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bioRxiv - Neuroscience 2020Quote: ... Biotinylated proteins were then purified using high capacity neutravidin-conjugated beads (Thermo Fisher Scientific) via incubation for three hours at 4°C after which beads were washed extensively in dilution buffer plus 0.5M NaCl ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR products were purified and cloned into the pDONRP4P1r vector (Thermo Fisher Scientific). The promoter fragment was then assembled with GAL4 into the pB-9FH2A-UAS-7m24GW destination vector in a multi-site gateway reaction to create an activator line construct using the LR Clonase II+ (Thermo Fisher Scientific) ...
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bioRxiv - Pathology 2021Quote: Total RNA was isolated and purified from lung tissues using Trizol Reagent (Invitrogen, CA). cDNA was obtained by reverse-transcribing the purified RNA using the first stand cDNA synthesis kit (Thermofisher ...
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bioRxiv - Microbiology 2020Quote: ... Fabs were expressed in ExpiCHO cells and purified using CaptureSelect CH1-XL resin (ThermoFisher) and followed by size exclusion chromatography ...
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bioRxiv - Molecular Biology 2020Quote: ... We sometimes also purified RNA probes using a 2.5x volume of AMPure beads (ThermoFisher 09-981-123 ...
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bioRxiv - Neuroscience 2021Quote: ... RNA was further purified for polyadenylated RNA by using Dynabeads mRNA Purification Kit (Ambion). The libraries were prepared by TruSeq RNA Sample Preparation Kit v2 (Illumina ...
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bioRxiv - Bioengineering 2019Quote: ... USA). GeneArt Platinum Cas9 nuclease purified protein (cat. nr. B25642) was purchased from Invitrogen (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... and purified using the Gene Jet RNA Cleanup Kit (Thermo Fisher Scientific, Waltham, MA). RNA quality and quantity were assessed using a Nanodrop 2000 (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... purified AHK5RD as a target was labelled with Alexa Fluor-488 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Purified coronavirus spike ectodomains were coated onto 96-well NUNC Maxisorp plates (Thermo Scientific) at equimolar amount at room temperature (RT ...
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bioRxiv - Physiology 2021Quote: Total RNA from the gravity-settled cardiomyocytes was then purified using TRIzol reagent (Invitrogen) followed by digestion with on column-RNase-free DNAse digestion kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... Amplicons were purified using the Axygen AxyPrep Mag PCR Clean-up Kit (Thermo Scientific), and eluted into 30 μL molecular biology grade water ...
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bioRxiv - Neuroscience 2020Quote: ... The nucleic acids solution was purified from DNA using TURBO DNA-free Kit (Thermofisher). The mRNA obtained was then retrotranscribed using the RT reverse transcription Kit (Thermofisher) ...
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bioRxiv - Cell Biology 2021Quote: ... The antibodies were purified via negative selection against non-acetylated peptide ARLDHKFDLMYAK (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... Purified PCR products were cloned into pCR™-Blunt II-TOPO® vectors (Invitrogen) and sequenced (GenHunter Corp.).
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bioRxiv - Microbiology 2021Quote: ... The peptides were purified in SOLAμ HRP 2mg/1ml 96 well plate (Thermo Scientific) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Purified libraries were quantified using a Qubit 2.0 fluorometer (Life Technologies, Carlsbad, CA, USA) and confirmed using a 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Bioengineering 2020Quote: ... and the material was further purified using a zeba spin column (Thermo Fisher Scientific). Labeled αCD8M and αCD8D nanobodies were stored in PBS at -20°C ...
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bioRxiv - Bioengineering 2020Quote: ... The concentration of purified RNA was measured by spectrophotometer (Nanodrop 2000c, Thermo Fisher Scientific). cDNA was then synthesized using the QuantiTect Reverse Transcription Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2022Quote: ... Purified PCR products were cloned into a pCR2.1-TOPO vector (Invitrogen, Carlsbad, CA, USA) and the insert sequences were determined by Sanger sequencing using Applied Biosystems™ 3130 DNA Analyzers (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... The labeled DNA fragments were purified with a GeneJet PCR purification kit (Thermo Fisher). 20 fmol labeled DNA (or 20 fmol labeled with 1,000 fmol unlabeled for cold competitor control ...
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bioRxiv - Microbiology 2022Quote: ... was purified using nickel sepharose beads (Ni-NTA, Thermo Fisher Scientific, Waltham, MA, USA) following the manufacture’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The ligation reaction was purified using the GeneJET Gel Extraction Micro Kit (Thermo Fisher). To transform the final construct ...
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bioRxiv - Immunology 2022Quote: The purified cDNAs were quantified by Qubit DNA High Sensitivity kit (Thermo Fisher Scientific). Then ...
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bioRxiv - Immunology 2022Quote: ... Capped mRNA was purified by reverse phase chromatography using POROS™ resin (Thermo Fisher) followed by tangential flow filtration (TFF) ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA was purified using lysis buffer containing Proteinase K (Thermo Fisher Scientific, BP1700-100) and genotyping PCR was performed using the primers described in Supplementary Table 1 ...
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bioRxiv - Immunology 2022Quote: ... or naïve donors were purified using the Pierce Protein G Spin Plate (Thermo Scientific). Briefly ...
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bioRxiv - Biochemistry 2022Quote: All samples were purified by UHPLC on a Dionex UltiMate3000 (Thermo Fisher Scientific, Germany) using an analytical Zorbax 300SB-C8 column (150 mm x 2.1 mm ...