Labshake search
Citations for Thermo Fisher :
2101 - 2150 of 7029 citations for Ammonium Polyphosphate phase II 02 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... and the second tube was cultured to early stationary phase (1.2-1.4 OD600) as measured in a Thermo Spectronic 200 (Thermo Fisher Scientific brand ...
-
bioRxiv - Microbiology 2021Quote: ... pneumoniae strains were grown overnight at 37°C to stationary phase in LB-Lennox broth (Fisher Scientific, BP1427-2) with constant agitation ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: Purified peptides were subjected to Edman degradation on a gas-phase sequencer model ABI 492 (Applied Biosystems, CA, USA). The phenylthiohydantoin (PTH ...
-
bioRxiv - Cell Biology 2021Quote: ... and colonies with at least eight-cell cluster were scored using Inverted Infinity and Phase Contrast Microscope (Fisher Scientific). Where indicated ...
-
bioRxiv - Cell Biology 2022Quote: Brightfield microscopy images (10x, 20x magnification) were taken before each passaged using inverted phase-contrast microscope (Fisher Scientific #11350119). Cell counts ...
-
bioRxiv - Biochemistry 2022Quote: ... The eluate was loaded onto a reversed-phase analytical column (50 µm × 250 mm, 2 µm, C18, Thermo Fisher) with an elution gradient of 4%-35% mobile phase B (80% acetonitrile + 0.1% formic acid + 20% water at a flow rate of 300 nL/min ...
-
bioRxiv - Immunology 2022Quote: ... Axenic amastigotes were generated by culturing stationary-phase promastigotes at 32°C in Grace’s insect cell culture medium (Invitrogen), pH 5.2 ...
-
bioRxiv - Neuroscience 2020Quote: ... Peptide separation was performed using a reversed-phase analytical column (Acclaim PepMap RSLC, 0.075 x 250 mm, Thermo Scientific) with a linear gradient of 4%-36% solvent B (0.1% FA in 98% ACN ...
-
bioRxiv - Microbiology 2020Quote: ... The peptides were loaded on a reversed-phase nano-trap column in mobile phase A (75μm i.d. × 2 cm, Acclaim PepMap100 C18 3μm, 100Å; ThermoFisher Scientific; # 164946) and separated over an EASY-Spray column ...
-
bioRxiv - Neuroscience 2021Quote: ... HeLa digest was fractionated into 8 fractions using a high pH reversed-phase peptide fractionation Kit (ThermoFisher, Cat. #84868). Twenty μg of HeLa digest was loaded to a C18 solid phase spin column and eluted with a serial concentration of ACN (5% to 50%) ...
-
bioRxiv - Microbiology 2020Quote: ... The reporter plasmids were extracted from either log or stationary phase cultures using a Qiagen Miniprep kit (ThermoFisher, Germany). The plasmids were analyzed on chloroquine gels (1.5% agarose ...
-
bioRxiv - Biochemistry 2021Quote: ... Offline fractionation by high-pH reversed-phase chromatography was carried out on an Ultimate 3000 HPLC (Thermo Fisher Scientific) equipped with a 15 cm long ...
-
bioRxiv - Microbiology 2022Quote: ... offline high pH reverse-phase fractionation was performed using a Hypersil Gold column (Thermo Scientific, P/N 25002-202130). The mobile phase A was water adjusted with ammonium hydroxide to pH 10 and B was 10 mM ammonium bicarbonate in 80% Acetonitrile (ACN ...
-
bioRxiv - Biochemistry 2022Quote: ... the peptides were separated by reversed-phase HPLC using an Easy-nLC 1000 Nanoflow chromatograph (Thermo Fisher Scientific, USA). The separation was carried out in a –adsorbent with a particle size of 3.6 μm and a pore size of 300 Å ...
-
bioRxiv - Cancer Biology 2020Quote: ... and were separated on a 50 cm reversed-phase liquid chromatographic column (0.075 mm ID, Acclaim PepMap 100, C18, 2 µm) (ThermoFisher). Chromatography solvents were (A ...
-
bioRxiv - Microbiology 2020Quote: ... with an alkaline mobile phase on an Ultimate U3000 LC system (Dionex) and Q Exactive Orbitrap MS (ThermoFisher®) operating in both positive and negative ion mode as previously described [38 ...
-
bioRxiv - Genomics 2020Quote: Low passage SH-SY5Y cells in the undifferentiated growth phase were transfected with the plasmids via Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... After centrifugation the aqueous phase was loaded onto RNA columns (PureLink™ RNA Mini Kit, Thermo Fisher Scientific, #12183025). On-column DNaseI digestion was performed using the PureLink™ DNase Set (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2020Quote: Random biotinylation of S proteins was conducted using EZ-Link NHS-PEG Solid-Phase Biotinylation Kit (Thermo Scientific #21440). 10ul DMSO were added per tube for making concentrated biotin stock ...
-
bioRxiv - Cell Biology 2021Quote: ... Centrifugation was repeated (27,000g for 20 min) and the resulting soluble phase was incubated with HisPur Ni-NTA resin (ThermoFisher) for 30 min at 4°C ...
-
bioRxiv - Microbiology 2021Quote: The reduced muropeptides were desalted by reverse-phase high performance liquid chromatography (rp-HPLC) on a C18 Hypersil Gold aQ column (3µm, 2.1×200mm; Thermo Fisher) at a flow rate of 0.4ml/min ...
-
bioRxiv - Cell Biology 2020Quote: ... peptides were analyzed by online C18 nanoflow reversed-phase nano liquid chromatography (Dionex Ultimate 3000 RS LC, Thermo Scientific) combined with orbitrap mass spectrometer (Q Exactive Orbitrap ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Reconstituted subfractions were separated using reversed phase LC on an Ultimate 3000 chromatography system (Thermo Scientific, Sunnyvale, CA, USA). Peptides were loaded onto a C18 trap column (Thermo Scientific ...
-
bioRxiv - Microbiology 2020Quote: RNA extracted from the KS79 WT strain grown to E and PE phase was treated with DNase (ThermoFisher Scientific). 1 μg of DNase treated RNA was reverse transcribed with Protoscript II (New England Biolabs ...
-
bioRxiv - Microbiology 2020Quote: Total RNA was extracted from 5 ml cells grown to log phase (OD600 ∼0.6) at 37°C using TRIzol Max Bacterial RNA Isolation Kit (Invitrogen). RNA was further purified using the RNeasy kit (Qiagen) ...
-
bioRxiv - Molecular Biology 2020Quote: ... USA) and SOLAμ™ Solid Phase Extraction (SPE) HRP 2mg/1ml 96-well plates were purchased from Thermo Fisher Scientific (Rockford IL ...
-
bioRxiv - Immunology 2020Quote: ... 1x106 CTL (5days after restimulation therefore in expansion phase) were washed and resuspended in 100μl Opti-MEM medium (Gibco) at RT with 2μg mCherry-tagged Granzyme B mRNA (square wave electrical pulse at 300V ...
-
bioRxiv - Molecular Biology 2022Quote: ... Samples were pooled and dried using a Speedvac (Thermo) and fractionated using high pH reversed phase fractionation (Thermo Scientific) following the manufactures directions.
-
bioRxiv - Cancer Biology 2022Quote: ... RNA was subsequently extracted by transferring the aqueous phase from the TRIzol-chloroform extraction to RNeasy columns (Invitrogen, 74104). The rest of the RNA extraction was performed according to the RNeasy manufacturer protocol ...
-
bioRxiv - Plant Biology 2022Quote: ... USA) and separation was performed using a reversed-phase Acclaim PepMap RSL column (75LLμm IDLL×LL15Lcm, 2Lμm; Thermo Scientific). The mobile phase was 0.1% formic acid (FA ...
-
bioRxiv - Microbiology 2022Quote: ... cells were pulse-chased with 100 µg x ml-1 fluid phase marker Dextran AlexaFluor 647 (Thermo Fisher Scientific) 16 h prior infection ...
-
bioRxiv - Plant Biology 2022Quote: ... Pooled samples were subjected to high pH fractionation using Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific) following manufacturer instructions ...
-
bioRxiv - Genomics 2022Quote: ... tryptic peptides (1 mg) were decomplexed by reversed phase nanoLC separation (Ultimate 3000 nanoRSLC; Thermo Fisher Scientific, Dreieich, Germany) using a trap column setup (2 cm length ...
-
bioRxiv - Neuroscience 2022Quote: Normal-phase chromatography was performed using a Vanquish LC system controlled by Chromeleon Software (version 7.2.9 Thermo Fisher Scientific) and coupled to an Orbitrap Q-Exactive High Field mass spectrometer controlled by Xcalibur software (version 4.1.31.9 Thermo Fisher Scientific) ...
-
bioRxiv - Neuroscience 2022Quote: ... and the lower organic phase (containing the hydrophobic ACSS2i) was dried using a ThermoSavant SC110A SpeedVac Plus (Thermo Scientific). Once ready to inject in the MS ...
-
bioRxiv - Plant Biology 2022Quote: Pteridines were separated using a gradient elution program on a reverse-phase Hypersil Gold C18 column (1.9 µm particle size, 50 mm x 2.1 mm I.D.) (Thermo Fisher Scientific). The gradient consists of a binary solvent system consisting of 0.1% (v/v ...
-
bioRxiv - Cell Biology 2022Quote: ... This combined sample was then subjected to fractionation using the high pH reversed-phase peptide fractionation kit (Thermo Fisher) for a final of six fractions for the nuclear eluates ...
-
bioRxiv - Systems Biology 2024Quote: ... 100ug of the mixture was fractionated using a high pH reverse phase fractionation kit (Thermo Fisher Scientific, Cat #84868) for proteomics ...
-
bioRxiv - Plant Biology 2024Quote: ... in H2OMilliQ (phase A) and 0.1% formic acid in acetonitrile (LC-MS grade; Fisher Scientific or HiPerSolv CHROMANORM, VWR) (phase B ...
-
bioRxiv - Microbiology 2024Quote: ... Peptide separation was performed using a reversed-phase analytical column (Acclaim PepMap RSLC, 0.075 × 250 mm (Thermo Fisher Scientific)) with a linear gradient of 4-27.5% solvent B (0.1% FA in 98% ACN ...
-
bioRxiv - Cancer Biology 2024Quote: ... and cell membranes were labeled with 5 µg/ml fluorescent disordered/nonraft phase marker FAST DiO (Thermo Fisher Scientific) for 10 min on ice ...
-
bioRxiv - Molecular Biology 2023Quote: ... The aqueous phase was transferred to a new tube and precipitated by adding 1.5 μl of GlycoBlue Coprecipitant (Invitrogen) and 50 μl of ice-cold isopropanol and incubating at room temperature for 15 min ...
-
bioRxiv - Molecular Biology 2023Quote: ... LC-MS/MS was carried out by nanoflow reverse phase liquid chromatography (Dionex Ultimate 3000, Thermo Scientific, Waltham, MA) coupled online to a Q-Exactive HF Orbitrap mass spectrometer (Thermo Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... Peptide separation was performed using a reversed-phase analytical column (Acclaim PepMap RSLC, 0.075 x 250 mm, Thermo Scientific) with a linear gradient of 4%-27.5% solvent B (0.1% FA in 98% ACN ...
-
bioRxiv - Plant Biology 2022Quote: ... An autosampler and reverse phase UPLC chromatography column (2.6 µ m Accucore RP-MS, 100 mm length x 2.1 mm i.d.; ThermoFisher Scientific) was used to separate ABA with an acetonitrile (2%-55% ...
-
bioRxiv - Cell Biology 2023Quote: Fluorescence and phase-contrast images were captured using EVOS FL Auto microscope (AMAFD1000; Thermo Fisher Scientific, Waltham, MA, USA). Colony size of mESCs was measured using ImageJ software version 1.52a (Wayne Rasband ...
-
bioRxiv - Cell Biology 2023Quote: Samples were pooled in equimolar ratio and subjected to High pH Reversed-Phase Peptide Fractionation kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... were used to amplify the flgB upstream phase variation region and CloneJET PCR Cloning Kit (Thermo Fisher Scientific Inc.) was used to create a vector that carrying phase ON (pJET-ON ...
-
bioRxiv - Microbiology 2023Quote: ... bacteria were grown to log phase in the presence of 1:200 pooled normal human serum (Thermo Fisher Scientific) to opsonize bacteria ...
-
bioRxiv - Bioengineering 2023Quote: ... Phase contrast images were captured of stained differentiated and undifferentiated controls using a EVOS VL Core (Thermo Fisher Scientific).