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Citations for Thermo Fisher :
2101 - 2150 of 10000+ citations for 7 METHOXY 4 4 DIMETHYL 3 4 DIHYDRO 2H NAPHTHALEN 1 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and all sections were counterstained with 4′,6-diamidino-2-phenylindole (DAPI, ThermoFisher, 1:1000).
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bioRxiv - Immunology 2021Quote: ... 836.31 and 0526.02) used 4-fold excess PGT151 mAb and 1:60 α-hIgG:PE (Invitrogen) pre-incubated for 60 minutes as the phycoerythrin signal ...
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bioRxiv - Molecular Biology 2021Quote: ... 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside in DMSO (0.1-1mM, AICAR, Fisher Scientific), or isoproterenol (5-25µM ...
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bioRxiv - Immunology 2020Quote: ... the sections were stained with DAPI (4′, 6-diamidino-2-phenylindole; 1 μg/ml; Invitrogen). In the immunofluorescence study ...
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bioRxiv - Cell Biology 2021Quote: ... PCR products were run on an agarose gel (1-4%) supplemented with SybrSafe (Thermofisher Scientific) for visualisation on a UV transilluminator ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclear staining was done with 4’,6-diamidino-2-phenylindole (DAPI) (1:1000; Thermo Fisher) for 5 minutes at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... overnight at 4° C and Alexa Fluor 488 anti-rabbit secondary antibody (Invitrogen; 1:500) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were counterstained with 4’,6-diamidino-2-phenylindole (DAPI, 1:1000; Invitrogen, Eugene, OR). The sections were mounted onto gelatinized slides and left to dry (at least 2 hours ...
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bioRxiv - Neuroscience 2022Quote: ... or PDS-95 were fixed with 4% formaldehyde in 1× PBS (Thermofisher; catalog no. 10010023) for 15 min at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... 1/4 concentration of both inhibitors and is supplemented with 2% ESC-qualified FBS (Gibco). After day 2 ...
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bioRxiv - Genetics 2024Quote: ... The nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (1 μg/mL, Invitrogen) for 5 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: THP-1 macrophages were then fixed with 4 % formaldehyde (formaldehyde 16% MeOH free, ThermoFisher Scientific) in phosphate buffered saline (PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Pre-cast NuPAGE 1 mm 4-12% Bis-Tris gels (Thermo Fisher Scientific; Cat# NP0322BOX) were loaded and run at 200 V and 140 mA for 55 minutes in 1X NuPAGE BOLT SDS Running Buffer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... A 4:1 composite medium of Dulbecco’s minimum essential medium (DMEM, Gibco, Invitrogen, NY, USA) and Medium 199 ...
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bioRxiv - Cell Biology 2023Quote: ... A 4:1 composite medium of Dulbecco’s minimum essential medium (DMEM, Gibco, Invitrogen, NY, USA) and Medium 199 ...
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bioRxiv - Developmental Biology 2023Quote: ... Tissues were then stained with 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, D1306, 1:5000) for five minutes to visualize DNA ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated in 4′,6-diamidino-2-phenylindole (DAPI) (1:1000) (Invitrogen, Carlsbad, CA, USA) for 10 minutes and washed with PBS again ...
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bioRxiv - Biochemistry 2022Quote: ... for 1 hour at 4°C and analyzed on an Attune Flow Cytometer (ThermoFisher Scientific).
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bioRxiv - Bioengineering 2023Quote: ... cell media was removed and 1 ml of 4 µm Calcein AM stain (Fisher Scientific) in PBS was added before being left to incubate for 30 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were then counterstained with 4’,6-diamidino-2-phenylindole (DAPI) (D1306, 1:10,000, Invitrogen) diluted in 1X PBST (1:10,000) ...
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bioRxiv - Bioengineering 2023Quote: ... we add 1 mL of 4% w/v paraformaldehyde (Thermo Scientific, Cat. No. AA47392-9M) to the apical side of the Transwell® and incubate the culture at room temperature (RT ...
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bioRxiv - Bioengineering 2023Quote: ... followed by incubation with 2 μM calcein-AM and 4 μM ethidium homodimer-1 (Invitrogen) for 30 min to examine viability ...
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bioRxiv - Bioengineering 2023Quote: ... 1% penicillin-streptomycin and 4 mM L-glutamine (all three from Gibco, Thermo Fisher Scientific). Cells were plated at a density of 120,000 per dish either on high precision #1.5 25 mm coverslips (Marienfeld ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 4 mg ml-1 collagenase IV (Thermo Fisher Scientific, Catalog no. 17104-019) and DNAse I (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... 1% penicillin-streptomycin and 4 mM L-glutamine (all three from Gibco, Thermo Fisher Scientific). Cells were plated at a density of 120,000 per dish either on high precision #1.5 25 mm coverslips (Marienfeld ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were seeded in a 4-chamber glass bottom dish (D35C4-20-1-N, Invitrogen) for confocal imaging or in a 60 mm × 15 mm dish (705001 ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1-20 µg of protein was loaded onto 4-12% NuPage BisTris gels (Invitrogen) for electrophoresis ...
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bioRxiv - Bioengineering 2024Quote: ... and Alexa Fluor 647 (4 µg/mL, A21244, Invitrogen and 1:500, #4410, Cell Signaling). To stain for nucleus and cytoskeleton ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclei were stained with DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate, 1:3000; #D3571, Invitrogen) then mounted on glass slides with Mowiol ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... PBS (pH 7.4) containing 2X ethidium homodimer-1 (4 μM; #L3224B, Thermo Fisher Scientific, USA) was added to the existing medium in each well at a 1:1 ratio ...
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bioRxiv - Microbiology 2024Quote: ... AF647-conjugated donkey anti-sheep IgG (Thermo Fisher, A-21448, 1:500, 4 µg/mL), CF568-conjugated donkey anti-goat IgG (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... AF647-conjugated donkey anti-rabbit IgG (Thermo Fisher, A-31573, 1:500, 4 µg/mL), AF647-conjugated donkey anti-sheep IgG (Thermo Fisher ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... using crude extracts from plant leaves and monitoring the quantity of 4-methylumbelliferone products generated from the substrate 4-methylumbelliferyl-β-D-glucuronide (Duchefa) on a fluorometer (Thermo Scientific fluoroskan ascent).
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bioRxiv - Cell Biology 2022Quote: ... primary antibody at a 1:500 dilution in 1% BSA for 90 min at room temperature or 24 hr at 4°C and was followed by incubation with Alexa Fluor 488 goat anti-rabbit secondary antibody (Invitrogen A11008; 4 μg/ml) in 0.2% BSA for 60 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... RNA quantity was measured using a Qubit 4 fluorometer (Qubit 4 fluorometer; Qubit dnDNA HS Assay Kit, Cat No. Q32854 Invitrogen, Eugene, OR, United States). RNA quality was assessed using an Agilent RNA Nano kit on a BioAnalyzer instrument (RNA 6000 Nano Kit ...
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bioRxiv - Neuroscience 2023Quote: ... Dissected retinas were either fixed in 4% formaldehyde (MilliporeSigma, Cat.# FX0415-4) in PBS for 2 hours at room temperature or lysed with RIPA buffer (Thermo Fisher Scientific, Cat.# 89900) containing protease and phosphatase inhibitors (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was extracted from 4 young (⩽32 years) and 4 older patient (⩾40 years) granulosa cell samples using TRIzol (Life Technologies, Carlsbad, CA, USA) as previously described.111,112 Further ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were postfixed in 4% paraformaldehyde overnight at 4 °C and cut into 50 µm sections on a cryostat microtome (Thermo Fisher, NX50, Waltham, MA). Mounted sections were imaged on a scanning confocal microscope (TCS SP5 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell extracts (20 µg/lane) were fractionated in 3%-8% or 4-12 % tris-acetate gel (NP0006, NuPAGE Life Technologies). All antibodies were used at the concentrations recommended by the manufacturers.
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bioRxiv - Cell Biology 2020Quote: ... and then loaded with fluorescent Ca2+ probes (3 μM of fura-2 AM or 5.69 μM of Fluo-4 AM, Life Technologies) in HBSS for 30 minutes at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... IP and lysate samples were separated by SDS-PAGE on precast 3-8% tris-acetate and 4-12% bis-tris acrylamide gels (Invitrogen) followed by transfer to 0.2 μm nitrocellulose membranes (Amersham) ...
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bioRxiv - Genetics 2020Quote: ... Samples were loaded on 4-12% NuPAGE Bis-Tris gels or 3-8% NuPAGE Tris acetate gels (Thermo Fisher Scientific) and transferred to the nitrocellulose membranes (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... 10 ul of each sample and 3% input of each cell lysate was loaded on a 4-12% Bis-Tris pre-cast SDS-page gel (Invitrogen) and transferred to a PVDF membrane (BioRad) ...
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bioRxiv - Neuroscience 2021Quote: ... filtered through a 70 µm nylon colander (Falcon) with 3-4 ml of Ca2+- and Mg2+-free HBSS medium containing 10 % fetal bovine serum (FBS, Gibco), 1 % MEM-vit (Gibco ...
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Endothelial transmigration hotspots limit vascular leakage through heterogeneous expression of ICAM1bioRxiv - Immunology 2022Quote: ... Samples were boiled at 95ᵒC for 3 minutes to denature proteins and separated on a 4-12% NuPage Bis-Tris gel (Invitrogen, NP0322BOX). Proteins were transferred using an iBlot Gel Transfer device (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... third instar larvae were dissected in zero Ca2+ HL-3 solution at room temperature and stimulated with a HL-3 solution of 90 mM K+/2 mM Ca2+/4 μM FM4-64 (Invitrogen) for 5 min to load FM4-64 dye into the boutons ...
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bioRxiv - Molecular Biology 2021Quote: ... COCS were incubated for 3 days at 4°C with secondary antibodies Alexa594-conjugated goat anti-rabbit IgG (Life Technologies) or Alexa647-conjugated goat anti-rat IgG (Life Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... and 100 µg/ml CHX) and centrifuged at 35,000 rpm for 3 hours at 4° C in a TH-641 rotor (Thermo Scientific) before collecting the monosome and polysome peaks (Fig 5E) ...
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bioRxiv - Neuroscience 2019Quote: ... worms were kept at 4°C for 3 hours in 2X SSC buffer before treatment with Prolong Diamond Antifade (ThermoFisher, cat # P36961 ...
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bioRxiv - Neuroscience 2019Quote: ... Six of the ganglia were immediately fixed in 4% paraformaldehyde (n = 3 animals) or placed in RNAlater (ThermoFisher, Cat. #AM7021) for RNA extraction.