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Citations for Thermo Fisher :
2101 - 2150 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPENTANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Laser Capture Microdissection (LCM; N=3; 7 µM glass slide coated with polyethylene naphthalate – ThermoFisher #LCM0522), multiplex or regular immunohistochemistry (N≥3 4 µM glass slide ...
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bioRxiv - Developmental Biology 2024Quote: ... follicles were incubated with 500 nM CellEvent™ Caspase-3/7 Green Detection Reagent (C10427, Invitrogen). Oocytes were stained with 150 nM Sir-Tubulin (CY-SC002 ...
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bioRxiv - Cell Biology 2021Quote: ... only 4 μL of 1 mM FM 4-64 (Cat. number T13320, Thermo Fisher, Waltham, MA) was injected ...
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bioRxiv - Microbiology 2020Quote: ... and resuspended in 0.1 ml of PBS supplemented with the lipophilic styryl membrane dye N-(3-triethylammoniumprpyl)-4-(p-diethylaminophenyl-hexatrienyl) pyridinium dibromide (FM4-64, Molecular Probes, Invitrogen; 10 μg.ml-1) (Pogliano et al. ...
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bioRxiv - Neuroscience 2022Quote: ... brain slices were washed (5 min at RT x 3 times) in PBS and incubated (overnight at 4°C) with streptavidin-Alexa 488 (ThermoFisher Scientific, S-11223, 1:500) in PBS-triton 0.05% ...
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bioRxiv - Physiology 2022Quote: ... The samples were then mixed with 1 mL of a 3:13 solution of Ehrlich’s reagent (1.5 g of 4-[dimethylamino] benzaldehyde [ThermoFisher]; 5 mL ethanol; 337 µL sulfuric acid) to isopropanol and incubated for 30 min at 58°C ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed 3 times in PBST and mounted in 4′,6-diamidino-2-phenylindole (DAPI)-containing ProLong Anti-fade Diamond mountant (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... The samples were washed again in PBS for 3×15 min and mounted in DAPI (4-,6-diamidino-2-phenylindole; Life Technologies, D1306) for 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... were separated using NuPAGE Bis-Tris gels (4 – 12 %) with 3-(N-morpholino)propanesulfonic acid (MOPS) or 2-(N-morpholino)ethanesulfonic acid (MES) running buffer (Thermo Fisher Scientific) followed by transferring to polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2022Quote: N2A cells were transfected as described above with BicD2-KIF tail fusions and MBNL-GFP proteins at a ratio of 2:3 in 4-well chamber slides (Thermo Fisher, 154526). 24 hours after transfection ...
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bioRxiv - Neuroscience 2022Quote: ... goat anti-mouse Alexa-Fluor 488, 1:500, Thermofisher Scientific, 4 hrs at RT; Th: goat anti-rabbit Alexa-Flour 488, 1:500, Thermofisher Scientific, 4 hrs at RT). Sections were mounted on gelatin subbed slides and coverslipped with VectaShield anti-fade mounting medium (Vector Labs).
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bioRxiv - Cell Biology 2020Quote: ... 2% BSA and 2 mM EDTA at 1 x 106 cells per 100 μl: anti-ITGA7 APC 1:500 (Invitrogen MA5-23555) and anti-SCA-1 FITC 1:50 (BD Pharmingen 557405) ...
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bioRxiv - Genetics 2020Quote: ... A single gRNA and single strand HDR oligo were co-transfected along with the gRNA (1-2 μg of each plasmid) into 1-2 × 106 WT ESCs using Lipofectamine 2000 (Invitrogen #1168-019) in a single well of a 6 well plate ...
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bioRxiv - Bioengineering 2024Quote: ... and HITI insert were first mixed with a ratio of 2:2:1 with the reagent P3000 (ratio of DNA: P3000 of 1:2) in Opti-MEM™ Reduced Serum Medium (Gibco™), and then added to Opti-MEM™ Reduced Serum Medium (Gibco™ ...
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bioRxiv - Genomics 2023Quote: ... coated dishes in N2 medium (high-glucose DMEM, 1% N-2, 2% B-27 and 1% penicillin/streptomycin, from Thermo Fisher Scientific) at a density of 2×105 cells/cm2 ...
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bioRxiv - Cell Biology 2020Quote: ... incubated with goat anti-rabbit Alexa 647 (1:500 or 1:1000, 2 hours, ThermoFisher) and in the case of biocytin filling ...
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bioRxiv - Developmental Biology 2021Quote: ... incubated with 2° antibody (1:1500) and Alexa FluorTM 647 Phalloidin (Invitrogen A22287, 1:2000) in PBTB for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... was dissolved in 0.25% acetic acid and diluted 1:1 with 2 x MEM (Gibco) to final concentration of 2.25 mg/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 × 106 cells were injected subcutaneously (resuspended in 1:1 media:Geltrex™ (Thermo Fisher A1413201)).
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bioRxiv - Cell Biology 2024Quote: ... Mouse IgG1 anti-alpha Tubulin Clone B-5-1-2 (1:500, ThermoFisher 32-2500), Rabbit anti-Myc polyclonal (1:500 ...
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bioRxiv - Microbiology 2023Quote: 1.5-2 μg total RNA was diluted 1:1 in 2x sample buffer (Invitrogen™), loaded onto 7.5% TBE-Urea PAGE gels ...
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bioRxiv - Cell Biology 2023Quote: ... For control knockdown a 1:1 mixture of Silencer Select Negative Control #2 (Ambion, 4390846) and Silencer Select Negative Control #1 (4390843 ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 µg/mL fibronectin in DMEM F12 with 1 % N-2 supplement (Invitrogen #17502048), 1 % L-Glutamine (Biological Industries #03-020-1B) ...
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bioRxiv - Cancer Biology 2021Quote: ... Drug dilution series (1:3) were prepared in PDAC organoid culture medium containing 1 μg/ml Hoechst (Invitrogen) and 1 μg/ml PI (Sigma) ...
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bioRxiv - Physiology 2022Quote: ... Plasmid DNA was mixed with PEI (1 mg/ml) at 1:3 in Opti-MEM medium (Gibco, 11058021) and added to cells after short incubation ...
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bioRxiv - Genetics 2022Quote: Calu-3 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM)/F12 (1:1) GlutaMAX™ (ThermoFisher Scientific) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... were added to the tumoroids in a 1:3 and 1:10 effector:target (E:T) ratio in RPMI 1640 (Gibco) supplemented with 10% FBS ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with 3 μg mRNA at a mass ratio of 1:1 using Lipofectamine MessengerMAX (Invitrogen) according to the manufacturer’s guide ...
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bioRxiv - Neuroscience 2024Quote: ... stained with TO-PRO-3 diluted 1:1000 in DPBS and 1 µM Hoechst 33342 (Thermo Fisher 62249) nuclear counterstain for 30 minutes at room temperature ...
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bioRxiv - Plant Biology 2019Quote: ... proteins were hydrolyzed into their protein-bound amino acids in a sealed glass tube at 150 °C for 2 h with 500 μL methanesulfonic acid 4 M (Fisher Scientific; Hampton, New Hampshire, USA) after flushing out air with a gentle stream of nitrogen gas ...
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bioRxiv - Cell Biology 2020Quote: ... 2 μM Rhod-2 AM (Thermo, R1244) and 2 μM Calcium green-1 AM (Life Technologies, C3011MP) was added to culture media and allowed to stain at 22 °C for 20 min prior to imaging (frozen Rhod-2 AM aliquots were used only when the DMSO suspension remained clear) ...
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bioRxiv - Immunology 2022Quote: ... mBECs treated with IFNγ were fixed with 4% paraformaldehyde in PBS for 15 minutes and stained with antibodies against Zona occludens-1 (ZO-1, 1:250 Invitrogen 33-9100) and CXCL10 (1:500 Abcam ab9938) ...
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bioRxiv - Genetics 2024Quote: ... and these primary antibodies were detected by the secondary antibodies Goat anti-Rabbit IgG (H+L) - Alexa Fluor 555 and Goat anti-Mouse IgG (H+L) - Alexa Fluor 488 (Invitrogen, A11001, 1:4000), respectively ...
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bioRxiv - Physiology 2023Quote: ... samples were further incubated with secondary antibodies (donkey anti-rabbit IgG H+L Alexa Fluor 546 and donkey anti-goat IgG H+L Alexa Fluor 488, both at 1:1000, Thermo Fisher Scientific, USA) at 4 °C overnight and mounted with fluorescence mounting medium (Agilent ...
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bioRxiv - Molecular Biology 2022Quote: ... siM1-4: 5’ -ACTGGTAGCTTATTAAAGATT- 3’) and the HOXA1 siRNA (5’ - AGAACTTCAGTGCGCCTTATT- 3’) were purchased from Invitrogen™ (Silencer® Select siRNA ...
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bioRxiv - Cell Biology 2020Quote: ... or on YPD plates (1% yeast extract, 1% peptone and 1% glucose) containing 2% agar supplemented with 100 µg/mL zeocin (Invitrogen), 300 µg/mL hygromycin B (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... the inoculum was removed, and 2 mL overlay media was applied (MEM with 10% FBS containing 1% methylcellulose, 1% HEPES, 1% GlutaMAX (Gibco), and 1% Pen/Strep ...
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bioRxiv - Developmental Biology 2021Quote: ... 4° C following by re-suspending the cells in 0.5-1 of ml cell isolation media (2% FCS, DPBS:HBSS=1:1 and 1mM SYTOX Blue Dead Cell stain (ThermoFisher Scientific). Cells were imaged before FACS and after FACS to confirm successful purification of GFP+ cells.
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bioRxiv - Microbiology 2021Quote: ... 6-diamidino-2-phenylindole (DAPI) at 1 μg mL−1 concentration and 50 μL of Film Tracer 1-43 membrane marker (Invitrogen) at 1 μg mL-1 concentration in DMSO ...
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bioRxiv - Neuroscience 2022Quote: ... cultures were incubated in rabbit anti-RIM 1/2 antibody (1:200) followed by donkey anti-rabbit AF647 antibody (1:500; Invitrogen).
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bioRxiv - Bioengineering 2024Quote: ... Cells were transfected at a concentration of 106 cells ml-1 with plasmid DNA (1 mg l−1) and 25 kDa linear polyethyleneimine (2 mg l−1) in OptiPRO SFM (Gibco). After 6 d of culture ...
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bioRxiv - Cell Biology 2024Quote: ... plates were prepared with 2 % agar in YPD (1 % yeast extract, 1 % peptone, and 1 % glucose) supplemented with 100 μg/mL zeocin (Invitrogen), 300 μg/mL hygromycin B (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... 47,5% DMEM/F12, 2% B27 w/o Vitamin A, 1% N2 supplement, 1% GlutaMAX, 1% Penicillin/Streptomycin; all from Gibco) supplemented with 10 µM SB-431542 (Torics Bioscience) ...
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bioRxiv - Neuroscience 2023Quote: ... six-well plates containing prewarmed neural differentiation medium (NDM; Neurobasal A, 2% B27, 1% N2, 1% Glut Amax, 1% P/S; all from Gibco) supplemented with 20 ng/mL of EGF and FGF2 (both PeproTech ...
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bioRxiv - Neuroscience 2023Quote: ... 47,5% DMEM/F12, 2% B27 w/o Vitamin A, 1% N2 supplement, 1% GlutaMAX, 1% Penicillin/Streptomycin; all from Gibco) supplemented with 10 µM SB-431542 (Tocris Bioscience) ...
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bioRxiv - Neuroscience 2023Quote: ... the spheroids were transferred to non-adhesive 100 mm dishes containing Neural differentiation medium (NDM; Neurobasal A, 2% B27, 1% N2, 1% GlutaMAX, 1% P/S all from Gibco) supplemented with 20 ng/mL of EGF and FGF2 (both PeproTech ...
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bioRxiv - Neuroscience 2020Quote: ... 4 µg of cDNA was transfected using a 1:1 ratio of cDNA: Lipofectamine 2000 (52887, Invitrogen) in OPTI-MEM transfection medium ...
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bioRxiv - Neuroscience 2020Quote: ... 4 μg of cDNA was transfected using a 1:1 ratio of cDNA: Lipofectamine 2000 (52887, Invitrogen) in OPTI-MEM transfection medium ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and washed once with 1 mL of EB and 4 times with 1 mL of PBS (Gibco). The sRNA were extracted from total/inputs and immunoprecipitated fractions using respectively Trizol and Trizol-LS ...
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bioRxiv - Developmental Biology 2021Quote: ... supplemented with 1% N-2 supplement (Invitrogen cat # 17502048), 1% Chemically Defined Lipid Concentrate (Invitrogen cat # 11905031) ...