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Citations for Thermo Fisher :
2101 - 2150 of 4311 citations for 6 Nitroisoquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... cells were plated into 6 well plates (0.5×106 cells per well) and cultured in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2022Quote: ... CC3 iPSCs were maintained in E8 medium on 6-well tissue culture plates (Fisher Scientific #07-20083) coated with growth-factor reduced Matrigel (Corning #354230) ...
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bioRxiv - Neuroscience 2022Quote: ... Real time quantitative PCR was carried out with QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) using TaqMan Universal PCR master mix (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclei were visualized with Hoechst (4′,6-diamidino-2-phenylindole) (DAPI) (Invitrogen; 3258, ICC/IHC 1:5,000).
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bioRxiv - Microbiology 2022Quote: Vero cells were grown on 6-well plates and were transiently transfected with Lipofectamine 2000 (Invitrogen, 11668019) based on the manufacturer’s protocols ...
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bioRxiv - Physiology 2022Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole (1:2500, DAPI, ThermoFisher Scientific, Cat No. D1306). Images were taken using Zeiss 880 confocal microscopy and quantified by using Fiji/ImageJ86.
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bioRxiv - Immunology 2022Quote: ... HT29 and SW480 cells were transfected in 6-well plate in presence of Lipofectamine 2000 (Life Technologies), 1μg Cas9+sgRNA plasmid (Mata-Garrido et al ...
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bioRxiv - Physiology 2022Quote: ... and primers listed in Table S3 and amplification was detected on the QuantStudio 6 Flex (Thermo Fisher). Gene expression was normalized to 18s ribosomal RNA expression and analyzed in QuantStudio (Thermo Fisher).
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bioRxiv - Cell Biology 2023Quote: ... sections were counterstained with 0.2 µg/ml 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Cat# 62248) diluted in 1xPBS for 15-30 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... cell nuclei were stained with DAPI (4’,6-diamidino-2-phenylindole, Thermo Fisher Scientific, 1:5000 dilution) for 15 mins ...
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bioRxiv - Cell Biology 2023Quote: ... Hepa 1-6 and HuH-7 were grown in Dulbecco’s modified Eagle’s medium–high glucose (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2023Quote: ... and serum IL-6 and TNF-α levels were measured using a commercial ELISA kit (Thermo Fisher). Serum CD5L levels were measured using ELISA ...
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bioRxiv - Plant Biology 2022Quote: ... N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino) phenyl) hexatrienyl) pyridinium dibromide (FM 4-64; 50 μM) (Invitrogen) or propidium iodide (PI ...
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bioRxiv - Biochemistry 2022Quote: ... The quality of obtained microsomes was tested with 9-amino-6-chloro-2-methoxyacridine (ACMA; Invitrogen A1324) fluorescence quenching assays.
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bioRxiv - Neuroscience 2023Quote: ... Data collection and analysis were completed using a Quantstudio 6 Flex real-time PCR machine (Thermo Fisher). Results utilized relative HCMV UL123 quantities being normalized to relative cellular GAPDH quantities.
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bioRxiv - Molecular Biology 2023Quote: U2OS 2-6-3 cells (Shanbhag et al., 2010) were cultured in McCoy’s 5A (Modified) Medium (Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... in a QuantStudioTM 6 Flex Real-Time PCR system (Applied Biosystems®, ThermoFisher Scientific, Waltham, MA, USA). Primers were from Sigma-Aldrich (St ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative gene expression analysis was performed on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) using SYBR-green technology (ABI) ...
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bioRxiv - Cell Biology 2023Quote: ... Real-time PCR reactions were performed using SYBR green in Quant Studio 6 Flex from Applied Biosystems. The data were analyzed with Quant Studio real-time PCR software version 1.3 ...
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bioRxiv - Cell Biology 2023Quote: ... DAPI (4’,6-Diamidino-2-henylindole, dihydrochloride) was obtained from Invitrogen (Catalog: D1306, CAS:28718-90-3).
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bioRxiv - Microbiology 2023Quote: ... Quantitative real-time PCR was performed on a real-time PCR system (QuantStudio 6 Flex, Applied Biosystems) with Power SYBR Green RNA-to-CT 1-Step Kit (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... were isolated from embryos incubated for between 6 (E6.0) and 8 (E8.0) days and maintained in chilled Leibovitz’s L-15 media (GIBCO, Invitrogen). Papillae were dissected as described previously 65 and cultured nerve-side-down on Millicell cell culture inserts (Millipore ®) ...
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bioRxiv - Biochemistry 2023Quote: ... The slides were then incubated with 1 ug/ml of 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen) in 1xPBS for 20 min at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were seeded onto 6-well plates forward transfected with Lipofectamine™ RNAiMAX transfection reagent (Invitrogen, # 13778150) according to manufacturer′s instructions using 30 pmol of siRNA for 72h at 37°C before harvesting ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclear stain: 4′,6′-diamidino-2-phenylindole dihydrochloride (DAPI, blue; 2 ng ml−1; Molecular Probes, USA). Sections were cover-slipped using ProLong Gold anti-fade reagent (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... 250 ng/ml 4′,6-diamidino-2′-phenylindole dihydrochloride (DAPI) or 500 nM SYTOX Orange (Life Technologies, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... FUCCI-hESCs cells were seeded into 6 well plates using medium without Penicillin-Streptomycin (Pen/Strep, Gibco), in low density ...
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bioRxiv - Bioengineering 2023Quote: ... After another set of gentle washes and 4′,6-diamidino-2-phenylindole (DAPI) nuclear staining (Thermo Scientific, R37606 ...
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bioRxiv - Cancer Biology 2023Quote: ... MM099 and MM029 were plated in 6-well plates and transfected with Lipofectamine 2000 (Thermo Fisher Scientific) according to manufacturer instructions either with 25 nM non-targeting siRNA control or with targeting siRNA (SMART-pool ...
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bioRxiv - Biophysics 2023Quote: ... Bead-bound protein was eluted by adding 6 μL of 4x LDS sample buffer (Thermo Fisher, NP0007) and heating the sample at 90 ºC for 5 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... 6 × 104 detached HeLa cells were transfected with 20 nM siRNA using Lipofectamine RNAiMAX (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Cells were seeded on 6-well plates and transfected 24 h later using Lipofectamine 3000 (Invitrogen, L3000150), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... The qPCR was performed using the QuantiStudio 6 Flex Real-Time PCR System (Thermo Fisher, CA, USA).
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bioRxiv - Cell Biology 2023Quote: ... wells were prepared with ice cold HBSS containing 6 µM FM1-43FX (Thermo Fisher Scientific Cat# F35355) with or without tubocurarine ...
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bioRxiv - Microbiology 2023Quote: ... Protein-nucleic acid mixtures were then loaded into either 6% or 10% Novex TBE gels (Thermo Fisher). Gels were pre-run for at least 30 min at 100V in 0.5x TBE buffer before sample loading ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were visualized with 4′,6-diamidino-2-phenylindole (DAPI) in SlowFade Diamond antifade mounting medium (ThermoFisher). Primary antibodies were incubated with tissues overnight at 4°C in 1% normal donkey serum ...
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bioRxiv - Synthetic Biology 2023Quote: Protein thermal stability was measured by differential scanning fluorimetry using a QuantStudio Pro 6/7 (Applied Biosystems). Protein was brought to a concentration of 10 uM with 5x Sypro Orange dye in a final volume of 20 µL in 20 mM NaPi ...
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bioRxiv - Molecular Biology 2023Quote: ... or electroporated into Hepa1-6 cells using a Neon Transfection System 10 µl kit (ThermoFisher MPK 1096) with the following electroporation parameters ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei staining was performed with 4’,6 diamidino-2-phenylindole dihydrochloride (DAPI, Molecular Probes, Eugene, OR, USA) at 300 nM in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... The sections were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; 0.02 mg/mL, ThermoFisher Scientific, USA), mounted and cover-slipped before being imaged with the Zeiss Axio Imager.Z1 Microscope ...
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bioRxiv - Neuroscience 2023Quote: ... the differentiation was initiated by changing the E8 to the Essential 6 (E6) medium (Thermo Fisher Scientific). E6 was fully refreshed every 24 hours for four days ...
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bioRxiv - Molecular Biology 2023Quote: ... qPCR was performed using a QuantStudio 6 Pro Real-Time PCR machine (Thermo Fisher Scientific, Waltham, MA). The following primers (5’-3’ ...
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bioRxiv - Molecular Biology 2023Quote: ... The transfection mixtures were replaced at 6 h post-transfection by 293 SFM II expression medium (Gibco), supplemented with sodium bicarbonate (3.7 g/L) ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were stained with DAPI (4’,6-diamidino-2-phenylindole, dihydrochloride) NucBlue (Cat. #: R37606; Thermo Fisher Scientific). Slides were mounted using Aqua-Poly/Mount (Cat ...
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bioRxiv - Immunology 2023Quote: ... 6 to 8 samples were pooled and loaded on the Ion 530™ Chip (Thermo Fisher Scientific) using the Ion Chef™ Instrument (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... After 6 hours the medium containing transfection reagents was replaced by FluoroBrite DMEM medium (Thermo Fisher, A1896701), supplemented with 10% (v/v ...
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bioRxiv - Biochemistry 2023Quote: ... and 2mM DTT) for 30 min at 4 °C and analyzed on 6% TBE gels (Life Technologies) at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... The pellet was dissolved in 50 μL of 6 M guanidine hydrochloride (GuHCL, Thermo Scientific™, AAJ6078622) and placed on the shaker for 20 min at 1000 rpm ...
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bioRxiv - Bioengineering 2023Quote: ... Bulk GBM cell lines (GBM-43, GBM-6) were harvested using 0.25% Trypsin-EDTA (Thermo Fisher Scientific) and passaged less than 30 times.
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bioRxiv - Neuroscience 2023Quote: The iPSCs and ESCs were maintained on 6-well plates coated with Geltrex (#A1413302, Thermo Fisher Scientific). For the initial 24 hours following seeding ...