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Citations for Thermo Fisher :
2101 - 2150 of 10000+ citations for 6 Methoxy 2 3 4 9 tetrahydro 1H b carbolin 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Invitrogen) and DiD (1,1’- Dioctadecyl-3,3,3’,3’-Tetramethylindodicarbocyanine, 4-Chlorobenzenesulfonate Salt; Invitrogen) crystals were inserted under a stereo fluorescence microscope (MZ10 F ...
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bioRxiv - Genetics 2023Quote: ... Cultures were passaged enzymatically every 3-4 days using 0.25% Trypsin (Gibco) diluted 1:3 in 1X PBS (Gibco) ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were passaged every 3 to 4 days using Accutase™ (Gibco) for up to 10 passages.
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bioRxiv - Genetics 2022Quote: ... Cells (1-2.5 x106/mL) were then cultured at 37 °C ex vivo for 2-6 hours in RPMI (Gibco, 21875-034) containing 10% FCS (IGC Technical Services ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected using 1-2 µg of pMX-GFP or pMX-53BP1 vectors and 6 µL Lipofectamine 2000 (Thermo Scientific). The media was changed after 3 hours of incubation ...
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bioRxiv - Bioengineering 2022Quote: ... The cell concentration was diluted 1:6 and cultured in 2 mL of Essential 8 (E8) - Flex Medium Kit (ThermoFisher Scientific) supplemented with 1% Penicillin Streptomycin (GIBCO ...
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bioRxiv - Developmental Biology 2024Quote: Total protein was extracted from 1-2 wells of a 6-well plate using RIPA lysis buffer with protease inhibitor (ThermoFisher Scientific). Protein concentration was measured using Pierce BCA kit (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.7 mL Buffer 2 (1 M sorbitol, 50 mM MES, pH 6, 5 mM MgCl2) plus protease inhibitors (Thermo Scientific A32955) was added ...
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bioRxiv - Microbiology 2021Quote: ... and mixed 1:1 with B-PER reagent (Thermo Fisher Scientific, Waltham, MA, USA). Cells were lysed by two rounds of sonification (Sonifier B-12 with microtip ...
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bioRxiv - Molecular Biology 2021Quote: ... one half of the Dicer-2 beads were incubated with 100 U RNase I (Ambion) in 100 µl IPP 150 at 25°C for 15 min on a thermomixer ...
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bioRxiv - Bioengineering 2022Quote: ... and 0.02 mM 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) (Invitrogen). The reaction was allowed to proceed on a rotary incubator at room temperature for at least 4 hours ...
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bioRxiv - Cell Biology 2019Quote: ... and 200 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDAC) (Invitrogen) in water for 1 hour at 60°C ...
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bioRxiv - Biophysics 2022Quote: ... 19 mg EDC (1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide) (Thermo Fisher), 11 mg sulfo-NHS (N-Hydroxysulfosuccinimide ...
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bioRxiv - Cell Biology 2023Quote: ... 1 % dipalmitoyl PI(3)phosphate (PI(3)P diC16) (Life Technologies) and extruded to 400 nm using a polycarbonate filter and a hand extruder (Avanti Polar Lipids ...
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bioRxiv - Bioengineering 2023Quote: ... and 0.02 mM 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) (Invitrogen). The reaction was performed on a rotary incubator at room temperature for at least 4 hours ...
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bioRxiv - Bioengineering 2024Quote: ... and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, Thermo Scientific, 22980) were added to the alginate solution in a molar ratio of 1 alginate:30 NHS:25 EDC ...
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bioRxiv - Microbiology 2023Quote: ... The cell lysate was mixed with 1 vol of lysis buffer B supplemented with 2% BSA and 50 µl of Dynabeads M-270 Streptavidin (Thermo Fisher Scientific, cat 65305) and incubated at 4°C for 30 min with rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... The 3×(CAC)2 and (CAC)2 RNAs were transcribed using T7 RNA polymerase (Thermofisher Scientific). A 10 μl binding reaction contains 10 nM RNA ...
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bioRxiv - Developmental Biology 2021Quote: ... Immunolabeling with performed with mouse anti-B23 primary antibody (1:300; 2h) then detected with Alexa 488 donkey anti-mouse antibody (Invitrogen; 1:500; 1h), then further fixed with 4% paraformaldehyde in DEPC-PBS ...
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bioRxiv - Genomics 2020Quote: ... After incubation for 1h with a secondary fluorescently labelled antibody in BS (1:1000) the tissue was stained with 1:100 YOYO1 (Molecular Probes, Life Technologies) for 15 min and mounted with Vectashield Antifade Mounting Medium (H-1000 ...
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bioRxiv - Neuroscience 2022Quote: ... following 1h incubation at RT with corresponding Alexa 488/Cy3/Cy5 coupled secondary antibodies (1:1000, Thermo Fisher Scientific). Cell nuclei were stained with DAPI ...
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bioRxiv - Cell Biology 2019Quote: ... Detection was performed with a 1h incubation at RT with secondary antibodies AlexaFluor488 or AlexaFluor594 (1:500; Molecular Probes), and DAPI (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... cells were exposed for 1h at RT to secondary antibodies: Goat anti-chicken AlexaFluor 488 (1:1000, ThermoFisher, A21131), Donkey anti-rabbit AlexaFluor 555 (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... Cell were incubated at 37°C for 1h in thyroid organoid media with mitotracker green 1:1000 (Invitrogen, M7514) and mitosox red 1:500 (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed 3 times with TBS-T and incubated 1h at RT with an HRP-conjugated anti-mouse secondary antibody (dilution 1:50,000, G-21040, Invitrogen). After 3 washes in TBS-T ...
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bioRxiv - Neuroscience 2024Quote: ... The secondary antibodies were diluted according to the manufacturer’s instructions and incubated for 1h at RT (488-ms/rb; 555-ms/rt; 647-ms, 1:1000, Invitrogen). Nuclei were visualized using 0,1μg/ml DAPI (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed three times within PBS and incubated for 1h at room temperature with the secondary antibody (Alexa-488 anti-mouse and anti-rabbit IgG, 1:2,000, Invitrogen; CY3 anti-rabbit ...
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bioRxiv - Developmental Biology 2024Quote: ... at a density of 9×104 cells ml−1 in N2B27 differentiation medium containing Advanced DMEMF12 and Neurobasal media (1:1, Life Technologies), B27 w/o vitamin A (Life Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: Glucose uptake experiments were performed using 2-NBDG (2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose) (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Glucose was traced using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, ThermoFisher, cat. #N13195) combined with 0,57 mg/mL 40kDa tetramethyl-rhodamine Dextran (Invitrogen ...
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bioRxiv - Immunology 2024Quote: In vivo labeling of tumor-infiltrating T lymphocytes (TILs) with 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino)-2-deoxyglucose (2-NBDG, Life Technologies, catalog: N13195) was done by intravenous (retroorbital ...
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bioRxiv - Cell Biology 2020Quote: ... A gradient consisting of 0% to 40% B in A (A: 0.1% formic acid [Fisher Scientific, A117], 6% acetonitrile [Fisher Scientific, A955] ...
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bioRxiv - Cell Biology 2019Quote: ... conjugated with GFP at the C-terminus (Par-6-GFP and aPKC-GFP) was inserted into pAc5.1/V5-His B plasmid (Invitrogen, Thermo Fisher Scientific). To construct an expression vector for Par-3 under control of the Gal4-UAS system ...
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bioRxiv - Developmental Biology 2021Quote: ... Peptides were eluted along a 90 min gradient from 6 to 40% Buffer B (see StageTip purification) with the EASY□nLC 1,200 system (Thermo Fisher Scientific). Measurement was done on an Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... cells from 100 mL of culture were resuspended and chemically lysed for 30 min in 6 mL of B-PER II (Thermo Fisher) diluted to 1x with TEMB buffer (10 mM Tris pH 8.0 ...
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bioRxiv - Neuroscience 2021Quote: ... propanal (ACROS Organics, 1%, CAS# 123-38-6), and 1,4-diaminobutane (ACROS Organics ...
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bioRxiv - Immunology 2020Quote: ... 6-incubation with 1 μg/mL DAPI (Invitrogen) and 2 μg/mL Wheat Germ Agglutinin (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 ng/ml IL-6 (Invitrogen, PHC3011)) (Wimmer ...
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bioRxiv - Developmental Biology 2023Quote: α6-FITC (1:200, Invitrogen, 11-0495-82),
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bioRxiv - Molecular Biology 2022Quote: ... and anti-G3BP-2 (Invitrogen, PA5-53776; at a 6 : 10 000 dilution), followed by three 2-min washes with room-temperature TBST ...
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bioRxiv - Developmental Biology 2022Quote: ... 5-(and-6)-chloromethyl-2′,7′ dicholorodihydrofluorescein diacetate (CM-H2DCFDA; Molecular Probes C6827), was used to visualize ROS accumulation (excitation ...
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bioRxiv - Immunology 2022Quote: ... or 2.5μM of 5-(and-6)-Carboxy-2’,7’-Dichlorofluorescein Diacetate (DCFDA) (Invitrogen) was then added and incubated with the cells 20 minutes at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... 6-diamidino-2-phenylindole (DAPI; Life technology) and mounted with antifade reagent (Invitrogen). The fluorescence microscopic images were acquired using the Leica TCS SP5 confocal microscope (Leica Microsystems ...
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bioRxiv - Cell Biology 2022Quote: Live cells were stained with Laurdan dye (6-dodecanoyl-2-dimethylaminonaphthalene) (Thermo Scientific) at 15 μM for 45 min ...
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bioRxiv - Cell Biology 2020Quote: ... 6 dpf/2 dpi larvae were pulsed with 2.5 nM EdU (Thermo Fisher) for 1h and chased for a further 1.5 h prior to fixation ...
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bioRxiv - Cell Biology 2023Quote: ... and 6-diamidino-2-phenylindole (DAPI) were obtained from Invitrogen (Carlsbad, CA, USA). General laboratory chemicals and biochemicals ...
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bioRxiv - Biophysics 2023Quote: ... 6-lauroyl-2-dimethylaminonaphthalene (Laurdan) was obtained from Molecular Probes (Eugene, OR, USA). HEPES was obtained from Fluka (Buchs ...
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bioRxiv - Cancer Biology 2023Quote: ... or 5- (and -6)-chloromethyl- 2′,7′-dichlorofluorescein diacetate (CM-H2DCFDA, ThermoFisher, C6827), or hydroxyphenyl fluorescein (HPF ...
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bioRxiv - Molecular Biology 2020Quote: ... and 0.25 μg/mL Amphotericin B (1% antibiotic/antimycotic; Gibco 15240112). Phlppi treatments were added as indicated ...
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bioRxiv - Microbiology 2021Quote: ... 70-kD dextran-rhodamine B (1 mg/ml, Thermo Fisher Scientific), or Lysotracker (1 μM ...