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Citations for Thermo Fisher :
2051 - 2100 of 10000+ citations for Zika Virus NS1 Proteins Uganda Suriname Strains Duo Pack since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: ... RNA was reverse-transcribed and amplified using the TaqMan Fast Virus 1-Step Master Mix RT-qPCR kit (Life Technologies, Carlsbad, CA, USA) on the LC96 instrument (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... Synthesis of c DNA from 1000 ng of pooled total RNA was carried out by using 10 μM of reverse primer from each reconstructed RNA virus and RevertAid Reverse Transcriptase (Thermo Fisher scientific, USA) (Cat ...
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bioRxiv - Microbiology 2023Quote: ... and real-time RT-PCR was performed to detect the presence of influenza A virus (M gene) using AgPath-ID™ One-Step RT-PCR Kit (Thermo Fisher Scientific). Positive influenza samples were then selected for whole genome sequencing using next generation sequencing (NGS ...
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bioRxiv - Neuroscience 2023Quote: ... The infusion line was primed with a loading line solution that was identical to the virus suspension solution [Corning phosphate-buffered saline without Ca2+ or Mg2+ with 350 mM NaCl (Gibco, ThermoFisher Scientific, Waltham, MA) containing 0.001% F68 surfactant (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA concentrations of 30 samples were normalized to approximately 15 ng µl-1 and TSV was detected and quantified from 2 µl of sample using a TaqMan™ Fast Virus 1-Step Master Mix (Applied Biosystems™) following the OIE recommended protocol and triplicate standards (mean R2 = 0.93 ± 0.06 ...
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bioRxiv - Microbiology 2023Quote: ... in a total volume of 25 µL containing 5 µL of template employing Taqman Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, USA). Oligonucleotide primer and probe sequences and thermocycling conditions are detailed in Table S2 ...
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bioRxiv - Bioengineering 2022Quote: ... vRNA levels were determined by quantitative real-time PCR (qRT-PCR) using Applied Biosystems™ TaqMan™ Fast Virus 1-Step Master Mix (Thermofisher #4444432) and a primer-probe combination recognizing a conserved region of gag (GAG5f ...
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bioRxiv - Synthetic Biology 2024Quote: MetBo2 (polyoma middle T oncogene–induced mouse mammary tumor on a syngeneic Friend Virus B NIH Jackson (FVB) background)[23] cells were maintained in 1X Dulbecco’s Modified Eagle Medium (DMEM) (Thermofisher Scientific; Cat. No. 11995065) with 10 % Fetal Bovine Serum (FBS ...
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bioRxiv - Systems Biology 2023Quote: ... and yield was determined by quantitative real-time PCR using the TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems, Foster City, CA) and TaqMan Gene Expression Assay ...
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bioRxiv - Immunology 2024Quote: ... lentiviral particles were generated through the transient transfection of plasmid into 293T virus- producing cells (ATCC, Manassas, VA, USA) in the presence of Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA), vesicular stomatitis virus G (VSV-G) ...
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bioRxiv - Immunology 2024Quote: ... RT-qPCR was used to detect and determine virus titers using the AgPath ID one-step RT-PCR kit (ThermoFisher Scientific, Waltham, MA) from the cell culture supernatants as previously described [28] ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was reverse-transcribed using Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase (RT) according to the manufacturer’s instructions (Invitrogen, Thermo Fisher Scientific, cat# 28025013). For quantitative Real Time Polymerase Chain Reaction (RT-qPCR) ...
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bioRxiv - Immunology 2024Quote: ... and cDNA was reverse transcribed using the Moloney murine leukemia virus reverse transcriptase and random hexamer oligonucleotides for priming (Life Technologies, CA, USA). The expression of the genes HIF-1α and LDH-A was determined using the PCR SYBR Green sequence detection system (Eurogentec ...
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bioRxiv - Cancer Biology 2024Quote: One microgram of total RNA was reverse transcribed at 37□°C for 60□min in the presence of random hexamers and Moloney murine leukaemia virus reverse transcriptase (Invitrogen, Carlsbad, CA, USA). Specific oligonucleotide primers for
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bioRxiv - Microbiology 2020Quote: ... IAV (H1N1 PR8 strain) and SARS-CoV-2 were grown in Vero cells with VP-SFM medium (11681-020, Gibco). DENV ...
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bioRxiv - Genomics 2021Quote: ... isolated from brain and testes tissues each of XYRIII strain of mouse were reverse transcribed with the (SuperscriptII, Reverse Transcriptase enzyme, Invitrogen), using oligo (dT ...
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bioRxiv - Microbiology 2019Quote: ... The plasmids used in the control reactions were generated by segment-specific RT-PCR from clinical samples of B/Victoria and B/Yamagata strains from the 2012-2013 season followed by gel extraction and TOPO-TA cloning (Invitrogen). The sequence of each plasmid was determined by Sanger sequencing ...
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bioRxiv - Molecular Biology 2021Quote: 24h after seeding of Vero E6 cells (5×104 per 48 well) APN01 was mixed with viral particles of the indicated strains at the given concentrations in DMEM Medium (Thermofisher) containing 5% FBS in 100μl per well and incubated for 30min at 37°C ...
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bioRxiv - Microbiology 2021Quote: The mutated alleles were first PCR-amplified from the evolved strains with Phusion high-fidelity DNA polymerase (Thermo Scientific, Finland), using the primers listed in Table S2 ...
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bioRxiv - Microbiology 2020Quote: ... an overnight culture of the parent strain was grown in BD Bacto™ Todd Hewitt broth with horse serum (Invitrogen), then diluted 200-fold and incubated at 37°C ...
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Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cellsbioRxiv - Cell Biology 2022Quote: The yeast strain ino1Δ used in this study belongs to the Saccharomyces cerevisiae BY4741 background and was obtained from Invitrogen. Cells were maintained on YPD plates (2% bactopeptone ...
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bioRxiv - Immunology 2019Quote: ... adult females aged 6 to 11 days after eclosure were injected with fluorescent bioparticles (E. coli K-12 Strain Bioparticles TexasRed Conjugate; Invitrogen) or pH sensitive fluorescent bioparticles (pHrodo E ...
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bioRxiv - Biochemistry 2020Quote: Growth experiments of selected strains carrying FDH variants were performed by inoculating M9 medium with the respective strain at a starting OD600 of 0.01 in 96-well microtiter plates (Nunclon Delta Surface, Thermo Scientific) and carried out at 37 °C ...
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bioRxiv - Immunology 2019Quote: ... Macrophages infected with fluorescent Mtb strains were used for lysosomal localization by using 100nm Lysotracker–Red DND-99 (Invitrogen, USA) according to manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2019Quote: ... The yeast strain used in the current experiments was ySS1224 (genotype: BY4741, CDC19::CDC19-GFP-HIS3, Life Technologies, Zug, Switzerland)
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bioRxiv - Genetics 2021Quote: ... RNA was isolated and cDNA generated from 10 young adult animals per strain and condition using the Power SYBR Green Cells-to-Ct kit (Invitrogen) according to the method described by Chauve et al (Chauve et al ...
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bioRxiv - Developmental Biology 2021Quote: ... were amplified from the genomic DNA of the RW strain and were cloned into pCR Blunt II-TOPO vector (Invitrogen). pDestTol2pA2-charon:EGFP (charon:EGFP for short ...
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bioRxiv - Cell Biology 2021Quote: Gene deletions were generated by PCR amplification of the locus-specific KanMX cassette from template genomic DNA obtained from the relevant strains of the gene disruption collection (Invitrogen) using primers annealing upstream and downstream of the genomic loci and subsequent transformation into the W303 background ...
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bioRxiv - Neuroscience 2021Quote: ... inactivated mouse embryonic fibroblasts (EmbryoMax® PMEF, strain CF-1) in hESC medium consisting of Knockout DMEM (#10829-018, Invitrogen) with 20% Knockout Serum Replacement (#10828-028 ...
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bioRxiv - Bioengineering 2021Quote: ... The wild-type and drug-resistant IAV strains were propagated in MDCK cells cultured in DMEM supplemented with 1% bovine serum albumin (Gibco) and 2 µg/mL l-1-tosylamido-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin at 34 °C for 48 h ...
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bioRxiv - Microbiology 2021Quote: Verticillium dahliae strain JR2 (CBS 143773; Faino et al., 2015) was cultured on potato dextrose agar (PDA) (Oxoid, Thermo Scientific) at 22°C in the dark ...
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bioRxiv - Microbiology 2021Quote: Well-differentiated camelid AEC cultures were infected with 4000 PFU of MERS-CoV (strain EMC/2012, propagated on Huh7 cells diluted in Hanks balanced salt solution (HBSS, Gibco). The cells were washed with 100 μl of HBSS prior to infection (18) ...
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bioRxiv - Microbiology 2021Quote: ... if sequences diverged between strains) were codon-optimised for expression in human cells and synthesised by GeneArt (Thermo Fisher Scientific), with an optimal 5’ Kozak sequence and fused to an N-terminal FLAG epitope ...
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bioRxiv - Cell Biology 2021Quote: The laboratory strain of Leishmania donovani (Dd8) was routinely maintained in high glucose Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Life Technologies) supplemented with 10% of heat-inactivated foetal bovine serum (FBS ...
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bioRxiv - Evolutionary Biology 2022Quote: ... simulans mh genomic region covering the coding region and ~3000 bp upstream was PCR amplified from the strain w501 using oligos 2099/2100 and cloned into pCR-Blunt II-TOPO (Invitrogen). The sequence of sim-mh was confirmed by Sanger sequencing (using oligos 788 ...
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bioRxiv - Synthetic Biology 2022Quote: Transformations for plasmid targeting experiments were performed in chemically competent PIR1 cells containing pTarget (original PIR1 strain obtained from Invitrogen), using 25 ng of pCAST or pHELIX and 25 ng of pDonor ...
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bioRxiv - Microbiology 2022Quote: ... parahaemolyticus strains were grown in LB-Miller at 37°C and diluted in DMEM (Dulbecco Modified Eagle’s Medium, Invitrogen #11995) to generate a multiplicity of infection (MOI ...
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bioRxiv - Microbiology 2022Quote: ... All strains were washed with PBS and diluted in Dulbecco’s Modified Eagle Medium (DMEM) without phenol red (Gibco, Life technologies). Number of bacteria were quantified by absorbance measurement at OD600 with an estimation of 2.5×108 bacteria in 1 ml of 1.0 OD600 ...
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bioRxiv - Microbiology 2021Quote: ... velezensis strains were prepared and inoculated into the wells of 24-well polyvinylchloride (PVC) microtiter plates (Fisher Scientific, Shanghai, China) filled with MSgg medium as described previously (52) ...
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bioRxiv - Microbiology 2020Quote: ... dahliae strain JR2 (CBS 143773; Faino et al., 2015) was maintained on potato dextrose agar (PDA) (Oxoid, Thermo Scientific, CM0139) and grown at 22°C in the dark ...
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bioRxiv - Microbiology 2021Quote: Two-day biofilm formation of Pseudomonas strains was determined by crystal violet staining using a 96-well plate lid with pegs extending into each well (Nunc-TSP lid ...
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bioRxiv - Microbiology 2022Quote: ... All strains were washed with PBS and diluted in Dulbecco’s Modified Eagle Medium (DMEM) without phenol red (Gibco, Life technologies). Number of bacteria were quantified by absorbance measurement at OD600 with an estimation of 2.5×108 bacteria in 1 ml of 1.0 OD600 ...
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bioRxiv - Microbiology 2019Quote: ... rodA2 and rodA3 in the different strains was assessed using the TaqMan® probe-based gene expression assay (Applied Biosystems). Expression of gyrB was used as control ...
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bioRxiv - Microbiology 2019Quote: ... Standard curves for gfp and oriV were constructed using plasmid isolated from the ancestral strain using the PureLink Plasmid DNA Miniprep Kit (Invitrogen). Total DNA amounts were quantified using a Qubit Fluorometer (ThermoFisher).
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bioRxiv - Cell Biology 2021Quote: ... All HTM cell strains used in this study were validated with dexamethasone (DEX; Fisher Scientific, Waltham, MA, USA; 100 nM) induced myocilin (MYOC ...
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bioRxiv - Biophysics 2021Quote: The DNA coding for NRMTs from 82 bacterial and archaeal strains were cloned from gDNA (obtained from DSMZ) into pBXNHG3 or pBXC3H vectors (Invitrogen) using FX cloning (Geertsma and Dutzler ...
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bioRxiv - Microbiology 2021Quote: ... The mycelia of the tested strains were then collected and preincubated for 15 min with 200 nM MitoTracker Red CMRos (M7512, Invitrogen). After washing with phosphate-buffered saline (PBS) ...
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bioRxiv - Bioengineering 2021Quote: ... All plates were spread with 150uL of the yMM1456 (suc2Δ leu2Δ) strain with glass beads (Fisher Scientific, #11-312B 4mm), the beads were removed and the plate allowed to dry for 10 minutes ...
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bioRxiv - Molecular Biology 2020Quote: The catalytic domain of the human ST6Gal I (residue range 132-406) was expressed in a BL21(DE3) Escherichia coli strain (Invitrogen), using a polycistronic Ptac vector containing the CyDisCo system (Gąciarz et al ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 3 wells from each strain were fixed with 10 µl of 16% paraformaldehyde (Fisher Scientific Cat. No. 50-980-487) and stored at 4°C ...