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Citations for Thermo Fisher :
2051 - 2100 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... proteins were subjected to a NuPAGE 12% gel (Invitrogen) and in-gel trypsin digestion was done on Coomassie-stained gel pieces with a modification ...
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bioRxiv - Molecular Biology 2024Quote: ... SYBR™ Gold Nucleic Acid Gel Stain (Thermo Fisher) was used for staining the gel ...
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Discovery and Optimization of Inhibitors for the Pup Proteasome System in Mycobacterium tuberculosisbioRxiv - Microbiology 2019Quote: ... Samples were boiled for 10 min before analysis by 10% SDS-PAGE gels (NuPAGE™ 10% Bis-Tris Protein Gels, 1.0 mm, Thermo-Fisher Scientific, Catalog # NP0301BOX). For immunoblot analysis ...
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bioRxiv - Immunology 2019Quote: ... PCR products were run at 100 V for 20 min on 2% (w/v) agarose gels and stained with SYBR Safe DNA Gel Stain (Invitrogen; Carlsbad, California, USA). Gels were visualised in a UV trans-illuminator and imaged using Quantum-Capture software.
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bioRxiv - Cell Biology 2019Quote: ... 100 µg were loaded onto a 1% agarose gel and ran on Owl™ EasyCast™ B1 Mini Gel Electrophoresis Systems (Thermo Scientific) at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... Electrophoresis was preformed through the stacking gel at 100 V and through the separating gel at 150 with PageRuler Prestained Protein Ladder (Thermofisher, Cat. No. 26620) as a marker ...
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bioRxiv - Microbiology 2020Quote: ... The phosphorylation of SUMO-DsbR within the gel was detected by using a Pro-Q™ Diamond Phosphoprotein Gel Staining Kit (Invitrogen, catalog#: MPP33300) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Purity of the protein was assessed by SDS-PAGE using NuPAGE™ Novex® Bis-Tris gels and blue native PAGE was performed by using the NativePAGE™ Novex® Bis-Tris Gel System and G250 additive (Life Technologies).
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bioRxiv - Microbiology 2020Quote: ... The gradient fractions were then analyzed by alkaline gel electrophoresis and visualized by SYBR Gold Nucleic Acid Gel Stain (Thermo Fisher, Cat# S11494). The peak fraction(s ...
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bioRxiv - Plant Biology 2022Quote: ... and proteins were loaded at equal Chl content in the native gel (NativePAGE™ 3 to 12%, Bis-Tris, 1.0 mm, Mini Protein Gel, 10-well from ThermoFisher catalog number BN1001BOX). Prior to loading the samples were supplemented with sodium deoxycholate (final concentration 0.3%) ...
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bioRxiv - Biochemistry 2022Quote: ... the PEG-tagged samples and the negative controls of the respective stable cell lines were resolved by denaturing gel electrophoresis on 3-8% Tris-acetate gradient protein gels (Thermo Fisher Scientific, EA0378BOX) using Tris-acetate-SDS running buffer ...
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bioRxiv - Immunology 2022Quote: ... The proteins were resolved by SDS-PAGE on 4-12% NuPAGE gels using NuPAGE MOPS buffer (NuPage gels, Thermo Fisher Scientific, Rockford, IL), and transferred to methanol-activated PVDF membrane (Perkin Elmer ...
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bioRxiv - Microbiology 2019Quote: ... The S5 datasets are distinguished by whether the libraries were size-selected using E-Gel SizeSelect II gels (SDG dataset, 300 bp; Invitrogen, Carlsbad, CA USA) or purified using standard AMPure XP bead cleanup (SDS ...
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bioRxiv - Microbiology 2021Quote: ... Bands of interest were excised from the gel and the DNA was purified using Thermo Scientific GeneJET Gel Extraction Kit (Thermo Fisher Scientific, USA) following manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... For sequencing a unique visible band in the agarose gel of ~700 bp was excised using a sterile bistoury and purified using GeneJET Gel Extraction Kit (Thermo Scientific™, USA). Finally ...
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bioRxiv - Cell Biology 2021Quote: ... and the soluble fractions were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on 6% Tris-Glycine gels (Life Technologies, Carlsbad, CA)64 ...
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bioRxiv - Molecular Biology 2019Quote: ... and the target size of the libraries (ca. 370-bp) was collected using 2% E-Gel Size Select Agarose Gels (Thermo Fisher Scientific, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Fifty μg of tissue homogenate were resolved with prefabricated polyacrylamide gel (BOLT 4-12% Bis-Tris Plus gel; Thermo Fisher Scientific, Monza, Italy) before electrophoretic transfer to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Synthetic Biology 2021Quote: ... and analyzed by SDS-PAGE (SDS-polyacrylamide gel electrophoresis) using Novex™ 14% Tris-Glycine protein gels (Cat. #XP00145BOX, Thermo Scientific, MA, USA). Protein bands were visualized with Coomassie Brilliant Blue staining.
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bioRxiv - Microbiology 2020Quote: ... The quality was assessed by running 5 μL of DNA template combined with 1 μL loading dye on a 0.3% agarose gel with 1 μL of 10,000x SYBR™ Gold nucleic acid gel stain (Invitrogen™, Cat. No. S11494) per 10 mL of gel at 3 V cm-1 for 90 minutes ...
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bioRxiv - Microbiology 2022Quote: ... The first round PCR sub-fragments were purified by extraction from an agarose gel using a GeneJET Gel Extraction Kit (Thermo Scientific™, ThermoFisher). A total mass of 10-15 ng of sub-fragments at a 1:1 molar ratio were then used as templates for OL-PCR using the forward and reverse outer primers ...
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bioRxiv - Cell Biology 2024Quote: ... 10-30 µg of protein was loaded onto precast gel (NP0321, NuPAGE™ 4- 12%, Bis-Tris, Mini Protein Gels, ThermoFisher Scientific, USA) and resolved at 135 volts for 90 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... 30 μg of protein per lane was loaded and run on a 4–12% Bis-Tris PAGE gel (Bolt 4–12% Bis-Tris Protein Gel, Invitrogen, catalogue no. NW04122BOX) and transferred onto a polyvinyl difluoride membrane (Immobilon-FL ...
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bioRxiv - Synthetic Biology 2023Quote: ... The second-round PCR products were size-selected using 2% agarose gel and purified using GeneJET Gel Extraction Kit (Thermo Fisher Scientific #K0691). The samples were pooled ...
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bioRxiv - Genomics 2022Quote: ... 15 μL of DNA extract with 1 μL of loading dye was run on a 0.3% agarose gel with 1 μL of 10,000x SYBR™ Gold nucleic acid gel stain (Invitrogen™, Cat. No. S11494) per 10 mL of gel at 3 V cm-1 for 90 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Transfer of proteins from SDS-PAGE gels to nitrocellulose membranes was performed by dry transfer using the iBlot™ 2 Gel Transfer Device (Thermo Fisher Scientific) following the manufacturers’ recommendations ...
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bioRxiv - Cancer Biology 2022Quote: ... The proteins in the lysates were separated by gel electrophoresis with Bolt™ 4-12% Bis-Tris Plus Gels (Invitrogen™, CAT: NW04122BOX) and transferred to Immobilon-FL PVDF Membranes (MilliporeSigma™ ...
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bioRxiv - Microbiology 2023Quote: ... 20μg of protein was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on NuPAGE 4-12% Bis-Tris gels (Thermo Fisher Scientific #NP0329). The gel was blotted onto Immobilon-FL PVDF Membrane (Millipore #IPFL00010 ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein samples were separated by SDS-PAGE ((4-20%; Criterion XT precast gel) with 1× MOPS SDS gel running buffer (NP0001, Invitrogen, Life Technologies Corp). Following electrophoresis ...
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bioRxiv - Bioengineering 2024Quote: ... 25 µg of proteins were separated in each well using 6% sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel with pre-stained molecular weight markers (ThermoFisher St. Louis, MO) and transferred to PVDF (polyvinylidene ...
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bioRxiv - Cell Biology 2024Quote: ... The cell lysates were cleared at 12.000 rpm at 4 °C for 10 min and 40 μg of proteins were loaded onto 10% polyacrylamide gel (Tris-glycine gels, Thermo Fisher Scientific, USA) and then transferred onto polyvinylidenefluoride (PVDF ...
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bioRxiv - Neuroscience 2024Quote: ... Protein lysates were then separated by gel electrophoresis on Bolt Bis-Tris Plus gradient 4-12% precast gels in MOPS/SDS running buffer in a Mini gel tank (Life Technologies, Carlsbad CA). Separation was performed for 60 minutes at 150V ...
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bioRxiv - Synthetic Biology 2020Quote: ... The sfGFP purification samples were combined and analysed via sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis (PAGE) using 4–12% Bis-Tris gels (NuPAGE Novex, Thermo Fisher Scientific, MA, USA), followed by western blot analysis using a HRP-conjugated GFP-specific polyclonal antibody (1:4,000 dilution ...
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bioRxiv - Genetics 2021Quote: ... and products were run on a 0.8 % agarose gel stained with Invitrogen™ SYBR™ Safe DNA Gel Stain (Life Technologies, Carlsbad, CA, USA). Template DNA carrying the mutant allele (A at nucleotide 585 ...
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bioRxiv - Genomics 2020Quote: ... amplified cDNA fragments were excised from the agarose gel and further purified using the PureLink® Quick Gel Extraction Kit (Life Technologies Corporation, USA). The gel extracted cDNA fragments were eluted with DEPC water to a final volume of 35 μL.
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bioRxiv - Cancer Biology 2020Quote: ... 10 of 50uL from the reaction volume was then heat-denatured in the presence of DTT and protein bands were detected by SDS-PAGE gel electrophoresis using a Tris-Glycine 10-20% gel (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Immunology 2020Quote: ... Samples were run on NativePAGE 3-12% BisTris Protein Gels using the dark blue cathode protocol for the NativePAGE™ Novex® Bis-Tris Gel system (Thermo Fisher Scientific). Gels were fixed ...
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bioRxiv - Genomics 2019Quote: ... Then the beads were eluted with water and the cleaned PCR product was run on the E-Gel® 2% agarose gel (Thermo Fisher Scientific, G501802) for further size selection and extraction ...
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bioRxiv - Molecular Biology 2021Quote: Lysates were loaded into either 10 % SDS-PAGE gels or pre-casted 4-12 % Bis-Tris 26-well NuPAGE™ or 10-well Bolt™ gels (Invitrogen, Thermo Fisher Scientific) and electrophoresed in either 1x SDS-PAGE running buffer or 1x MOPS running buffer ...
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bioRxiv - Bioengineering 2020Quote: ... was carried out under reducing and nonreducing conditions using Novex 12% Tris-Glycine Gels or Novex 16% Tricine Gels (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s recommended protocol and stained using Instant Blue Protein Stain (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... The linear vector was gel-purified (Monarch® DNA Gel Extraction Kit, New England Bioloabs) and quantified on a NanoDrop™ (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2019Quote: ... loaded on leaf area basis and separated by polyacrylamide gel electrophoresis (Nu-PAGE 4-12% Bis-Tris gel, Invitrogen, Life Technologies Corporation, Carlsbad, CA). Proteins were transferred to a nitrocellulose membrane and probed with antibodies against various photosynthetic proteins purchased from Agrisera (Vännäs ...
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bioRxiv - Genetics 2020Quote: Haplotype assignment for samples with a call of three across the TaqMan or Pharmacoscan CNV probes was conducted by L-PCR as described to generate an amplicon specific for the duplicated gene27 followed by gel extraction (GeneJET Gel Extraction Kit, Thermo Fisher Scientific, Waltham, MA) and the use of TaqMan Drug Metabolising assays specific for the haplotypes found in samples of heterozygous CYP2D6XN genotype by at least one platform (such as *2 ...
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ER exit sites in Drosophila display abundant ER-Golgi vesicles and pearled tubes but no megacarriersbioRxiv - Cell Biology 2021Quote: ... The resulting fragment was purified through gel extraction (Magen HiPure Gel Pure DNA Mini kit, cat # D2111-03,) and cloned into vector pDONR221 (Thermo Fisher Scientific, cat # 12536017) with GatewayTM BP ClonaseTM II Enzyme Mix (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated by their molecular weight using precast gradient polyacrylamide gels for improved separation of a wide range of proteins (NuPAGE® 4-12% Bis-Tris gels, Life Technologies, Darmstadt, Germany). Protein lysates were prepared by supplementation with the corresponding NuPAGE LDS Sample Buffer (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... based 4-20 % polyacrylamide (Rotiphorese Gel 30; Carl Roth) gradient gels and run (80-120 V; 90 min) in NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific; Cat#NP000102) using Mini-PROTEAN Tetra Vertical Electrophoresis Cells (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... correct size fragments corresponding to T/F LTR U3R regions and the linearized pGL3 Basic vector were gel extracted using the GeneJet Gel extraction kit (ThermoFisher Scientific, Waltham, MA, USA) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The 8-well chamber walls were placed around the gels and 150-200 µl of hydroxyethyl agarose processing gel (Thermo Fisher, HG 4000-012) was poured into the well ...
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bioRxiv - Bioengineering 2023Quote: ... Gels were loaded into an Invitrogen mini gel tank (A25977) and run with Bolt™ 1X MES SDS Running buffer (Life Technologies, Carlsbad, CA). Using a power ease 300W (Life Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by polyacrylamide gel electrophoresis (PAGE) using 4-12% gradient gels followed by protein transfer onto nitrocellulose membranes with iBLOT2 (Thermo Fisher Scientific, Waltham, MA). Membranes were blocked in 5% nonfat dry milk made in Tris-buffered saline with Tween-20 (TBST ...