Labshake search
Citations for Thermo Fisher :
2051 - 2100 of 4771 citations for TBK1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat productionbioRxiv - Bioengineering 2023Quote: ... and blocked for 45 minutes using a blocking buffer of DPBS with 5% goat serum (ThermoFisher #16210064) and 0.05% sodium azide (Sigma #S2002) ...
-
bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were diluted in 7.5ml Intercept PBS blocking buffer with 0.1% Tween (SynGAP1; 1:1000dil, Invitrogen, PA1-046 ...
-
bioRxiv - Neuroscience 2023Quote: ... incubated with Alexa 647 labeled donkey anti-rabbit secondary antibodies (1:250 in blocking solution; Invitrogen A32795TR) for 1 h at RT ...
-
bioRxiv - Neuroscience 2023Quote: ... A blocking solution was then added with 0.1% Triton-X-100 and 3% bovine serum albumin (Invitrogen). An antibody solution of MAP2 (1:2500 ...
-
bioRxiv - Microbiology 2023Quote: ... The membrane was blocked for 1 h at RT with nucleic acid detection blocking buffer (Life Technologies), then probed for 1 h at RT with streptavidin-HRP 1:5,000 (Genescript ...
-
bioRxiv - Developmental Biology 2023Quote: ... and incubated in blocking solution with goat or donkey raised Alexa Fluor-conjugated secondary antibodies (Thermo Fisher), at room temperature for 24 hours ...
-
bioRxiv - Pathology 2024Quote: ... HT7 was the only anti-mouse antibody used and a mouse-on-mouse blocking agent (Invitrogen: R37621) was applied for 30 minutes post protein block washes to reduce noise from secondary antibody binding and an additional three 5-minute washes were added before primary antibody incubation ...
-
bioRxiv - Neuroscience 2024Quote: ... BV-2 cells were fixed and sequentially incubated with Mouse-on-Mouse IgG Blocking Solution (Invitrogen R37621) and Navenci blocking solution at 37°C for 60 min each in a pre-heated humidified chamber ...
-
bioRxiv - Bioengineering 2024Quote: ... for 1 hour before blocking with 3% (w/v) bovine serum albumin (BSA, Fisher Scientific, Cat # BP9703100). To detect ARP3 and GLUT4 ...
-
bioRxiv - Cell Biology 2024Quote: ... The membranes were blocked with 1X BlockerTM FL 10X Fluorescent blocking buffer (Thermo Fisher Scientific, Cat # 37565) followed by incubation with the respective primary antibodies overnight at 4°C ...
-
bioRxiv - Immunology 2024Quote: ... The fixed slides were washed with PBS for twice and blocked with Blocking buffer (Thermo Fisher Scientific) for 1 h at room temperature ...
-
bioRxiv - Cancer Biology 2021Quote: ... Peptides were loaded onto the trapping column (PepMap100, C18, 300 µm x 5 mm, Thermo Scientific) using partial loop injection with 2% acetonitrile (ACN) ...
-
bioRxiv - Cancer Biology 2021Quote: ... fractionated into 8 fractions using the High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific 84868) according to the manufacturer protocol and dried ...
-
bioRxiv - Cancer Biology 2021Quote: Peptide mixtures were separated by reversed-phase chromatography on an EASY-Spray column (Thermo Fisher Scientic), 25-cm long (inner diameter 75 μm ...
-
bioRxiv - Biochemistry 2021Quote: ... with 15 µM Impβ mixed with 75 µM Rev-peptide and 1x SYPRO Orange (Molecular Probes) diluted in H2O ...
-
bioRxiv - Cell Biology 2020Quote: ... Cleaved peptides were analyzed by nanoflow LC-ESI-MS/MS (Thermo Scientific LTQ Orbitrap Velos Pro). Peptide sequences were mapped using ProteinProspector and Mascot software packages.
-
bioRxiv - Cell Biology 2020Quote: ... The isobaric labelling of the peptides was performed using 10-plex TMT reagents (Thermo Fisher Scientific).
-
REEP4 is recruited to the inner nuclear membrane by ELYS and promotes nuclear pore complex formationbioRxiv - Cell Biology 2020Quote: Peptides were analyzed by C18 nanoflow reversed-phase HPLC (Dionex Ultimate 3000 RSLC nano, Thermo Scientific) combined with orbitrap mass spectrometer (Q Exactive HF Orbitrap ...
-
bioRxiv - Microbiology 2021Quote: ... Peptides were loaded with the autosampler directly onto a 50 cm EASY-Spray C18 column (ThermoFisher). Peptides were eluted from the column using a Dionex Ultimate 3000 Nano LC system with a 14.8 min gradient from 1% Buffer B to 23 % Buffer B (100% acetonitrile ...
-
bioRxiv - Developmental Biology 2020Quote: ... desalting and fractionation was performed with Pierce High pH Reversed-Phase Peptide Fractionation Kits (Thermo Scientific) to generate 8 fractions per sample ...
-
bioRxiv - Developmental Biology 2022Quote: ... Peptide desalting was performed according to the manufacturer’s instructions (Pierce C18 Tips, Thermo Scientific, Waltham, MA). Desalted peptides were reconstituted in 0.1% formic acid in water and further separated into four fractions by strong cation exchange chromatography (SCX ...
-
bioRxiv - Molecular Biology 2020Quote: ... Peptide desalting was performed according to the manufacturer’s instructions (Pierce C18 Tips, Thermo Scientific, Waltham, MA). Eluates were lyophilized and reconstituted in 11 µL of 5% acetonitrile and 2% formic acid in water ...
-
bioRxiv - Microbiology 2021Quote: Peptides were labelled with Alexa Fluor® 488 (A488) 5-sulfodichlorophenol ester (SDP) (Thermo Fisher Scientific) using previously described methods [66] ...
-
bioRxiv - Immunology 2021Quote: ... The digested peptides were labelled with iTRAQ reagents according to the manufacturer’s protocols (Applied Biosystems, USA). The peptides from the Hemocyte samples collected at four different time points were labelled with four 8-plex iTRAQ reagents (Applied Biosystems ...
-
bioRxiv - Molecular Biology 2021Quote: ... Peptides were analyzed on a hybrid quadrupole orbitrap mass spectrometer (Orbitrap Exploris 480; Thermo Fisher Scientific) equipped with an Easy1200 nanoLC system (Thermo Fisher Scientific) ...
-
bioRxiv - Plant Biology 2020Quote: ... Peptides were eluted from a 75 µm x 50 cm analytical C18 column (PepMan, Thermo Scientific) on a linear gradient running from 4% to 64% acetonitrile over 135 min ...
-
bioRxiv - Microbiology 2019Quote: ... 0.8-3 μg peptide were loaded with an UltiMateTM 3000 RSLCnano Liquid Chromatograph (Thermo Fisher Scientific) in loading solvent A (2% acetonitrile ...
-
bioRxiv - Neuroscience 2019Quote: ... and treated with 10 μM of freshly prepared amyloid-β peptide 1-42 (Aβ42, Life technologies) for 24 h ...
-
bioRxiv - Molecular Biology 2019Quote: ... the peptides were trapped on an Acclaim PepMap C-18 precolumn (0.3×5mm, Thermo Fisher Scientific), using a flowrate of 25 µl/min and 100% buffer A (99.9% H2O ...
-
bioRxiv - Biochemistry 2019Quote: ... The peptides were analyzed by an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific, USA)[32] ...
-
bioRxiv - Cell Biology 2019Quote: ... Peptides (100ng) were separated on a Thermo ScientificTM EASY-nLC 1000 HPLC system (Thermo Fisher ScientificTM) for 1hour from 5-60% acetonitrile with 0.1% fromic acid and directly sprayed via a nanoelectrospray source in a quadrupole Orbitrap mass spectrometer (Q ExactiveTM ...
-
bioRxiv - Microbiology 2019Quote: ... Peptides were resolved on the analytical column (PepMap C18, 75 μm×50 cm, 2μ, Thermo Scientific) at 300 nL/min flow rate using a step gradient of 5% to 22% solvent B (0.1% formic acid in 95% acetonitrile ...
-
bioRxiv - Systems Biology 2019Quote: ... Peptides were analyzed using a Q Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher Scientific), equipped with a Nanospray Flex Ion Source (Thermo Fischer Scientific) ...
-
bioRxiv - Biochemistry 2019Quote: ... peptides were ionized by nanoelectrospray and analyzed using a QExactive HF-X mass spectrometer (ThermoFisher Scientific). The full MS-scan was followed by MS/MS for the top 15 precursor ions in each cycle ...
-
bioRxiv - Neuroscience 2019Quote: ... or 10 μM of freshly prepared human synthetic amyloid-β peptide 1-42 (Aβ42, Life technologies) for 24 h as described (Kiyota et al. ...
-
bioRxiv - Molecular Biology 2019Quote: ... Larval peptides were analyzed on an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific, Waltham, MA, USA) with a 4.5 cm ...
-
bioRxiv - Synthetic Biology 2019Quote: ... peptide separation was carried out using an EASY nLC-1000 system (Thermo Fisher Scientific, MA, USA) equipped with a RP-HPLC column (75 μm × 30 cm ...
-
bioRxiv - Developmental Biology 2021Quote: Peptides were separated using an Ultimate 3000 RSLC nano liquid chromatography system (Thermo Fisher Scientific, USA) coupled to an LTQ Velos Orbitrap mass spectrometer (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2020Quote: ... Cross-linked peptides were separated on an UltiMate 3000 RSLC nano HPLC system (Thermo Fisher Scientific) coupled to an Orbitrap Fusion Lumos Tribid mass spectrometer (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2020Quote: ... The proteomic analysis of the peptides was carried out on Q Exactive (Thermo Fisher Scientific, Inc.) equipped with UltiMate 3000 Nano LC Systems (Thermo Fisher Scientific ...
-
bioRxiv - Bioengineering 2020Quote: ... the cross-link peptide mixtures were desalted and analyzed with a nano-LC 1200 (Thermo Fisher) coupled to a Q Exactive™ HF-X Hybrid Quadrupole-Orbitrap™ mass spectrometer (Thermo Fisher) ...
-
bioRxiv - Biochemistry 2021Quote: ... The chemically synthesized p53 TAD2 domain peptide (38QAMDDLMLSPDDIEQWFTEDPGPD61) was obtained with >90% purity from Thermo Scientific, USA ...
-
bioRxiv - Neuroscience 2021Quote: ... TMT 10-plex labeling was performed on peptide samples according to the manufacturer’s instructions (ThermoFisher Scientific). After incubating for 2 hr at room temperature ...
-
bioRxiv - Cell Biology 2021Quote: ... Peptides were separated by liquid chromatography and analyzed on an Orbitrap Elite mass spectrometer (Thermo Fisher). Data were analyzed using Proteome Discoverer Software (Thermo Fisher).
-
bioRxiv - Biochemistry 2021Quote: Produced peptides of HTT were separated using a Vanquish Flex UHPLC (Thermo Fisher Scientific, Bremen, Germany) coupled on-line to an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2021Quote: ... Peptide analysis was performed in an LTQ Velos nano-ESI linear ion trap equipment (Thermo Scientific) in a data-dependent acquisition mode ...
-
bioRxiv - Biochemistry 2021Quote: ... The peptides were separated on an Ultimate 3000 RSLC nano-flow chromatography system (Thermo Fisher Scientific), using a pre-column for sample loading (PepMapAcclaim C18 ...
-
bioRxiv - Bioengineering 2021Quote: ... Peptide mixtures were trapped on a 2 cm × 100 μm Pepmap C18 column (Thermo Fisher 164564) and then separated on an in-house packed 50 cm × 75 μm capillary column with 1.9 μm Reprosil-Pur C18 beads (Dr ...
-
bioRxiv - Cell Biology 2021Quote: ... Peptides were extracted with ethyl acetate and desalted on C18-Stage tip columns (Thermo Fisher Scientific), eluates were dried in a SpeedVac and resuspended in 1% formic acid for loading ...
-
bioRxiv - Plant Biology 2020Quote: Peptides were analyzed using Easy nanoLC-II coupled with LTQ Velos Pro mass spectrometer (Thermo Scientific). Peptides (350 ng ...