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Citations for Thermo Fisher :
2051 - 2100 of 10000+ citations for Recombinant Mouse Hmgb1 Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... binding was quantified by measuring the fluorescence of a PE-conjugated anti-human Fc antibody (Invitrogen) detecting the Fc-fused protein target ...
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bioRxiv - Immunology 2023Quote: ... blocked with Fc-block and stained with LiveDead APCe780 (1:1000; Thermo Fisher, #65-0865-14), APC anti-CD19 (1:200 ...
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bioRxiv - Bioengineering 2023Quote: ... HRP-conjugated goat anti-human IgG-Fc (10000x dilution) was used for detection (Invitrogen Cat# 31413). Immobilon Western Chemiluminescent HRP Substrate (Millipore Sigma ...
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bioRxiv - Microbiology 2023Quote: ... prior to resuspension in complete RPMI (with 10% FCS, 1% Penicillin/Streptomycin, 1% L-glutamine, Gibco) and passing through a 70 μm cell strainer ...
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bioRxiv - Immunology 2023Quote: ... After treating the cells for 15 minutes (min) with Fc Receptor Binding Inhibitor Polyclonal Antibody (ThermoFisher), they were stained for 30 min at 4°C in the dark and washed twice again ...
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bioRxiv - Immunology 2023Quote: ... Optic density was then measured at 405 nm on a Multiskan FC microplate photometer (Thermo Scientific).
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bioRxiv - Bioengineering 2023Quote: Ly6a-Fc was produced in Expi293F suspension cells grown in Expi293 Expression Medium (Thermo Fisher Scientific) in a 37 °C ...
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bioRxiv - Bioengineering 2023Quote: Ly6a-Fc was produced in Expi293F suspension cells grown in Expi293 Expression Medium (Thermo Fisher Scientific) in a 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... medium of the HEK293T cells was changed to transfection medium containing DMEM and 10% FCS (Gibco), incubating the cells for 30 min at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... The tissue was incubated 2 times in RPMI 1640/5%FCS supplemented with 5mM EDTA (Gibco) for 20min each at 37°C and 200rpm ...
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bioRxiv - Bioengineering 2023Quote: ... The sections were then incubated with WA2.22 Fc fusion (100 nM) and AT8 (1:200, Invitrogen) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... KELLY cells were cultured in RPMI medium with 10-15% fetal calf serum (FCS) (Life Technologies). Cells were maintained at 5% CO2 and at 37°C and were split at full confluency (at 5% CO2 and at 37°C ...
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bioRxiv - Immunology 2023Quote: ... cells were first incubated with an anti-CD16/CD32 Monoclonal Antibody to block FC receptors (Invitrogen). Cells were washed using PBS and incubated with LIVE/DEAD™ dye for 30 minutes at 4°C ...
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: ... ALK1-Fc-complexes were then captured with CaptureSelect FcXL Affinity Matrix (Cat: 194328010, Thermo Fisher Scientific). The ALK1 ligand-depleted 30% BSA was diluted to 0.2% with phenol red-free EBM2 for use.
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bioRxiv - Immunology 2023Quote: ... Raji cells and CHO cells were cultured in complete RPMI-1640 (Gibco, 10% FCS, 2mM GlutaMAX), and HEK293T cells ...
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bioRxiv - Immunology 2023Quote: ... prior to resuspension in complete RPMI (with 10% FCS, 1% Penicillin/Streptomycin, 1% L-glutamine, Gibco) and passing through a 70 μm cell strainer ...
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bioRxiv - Microbiology 2023Quote: Isolated monocytes were resuspended in complete RPMI media with 2mM L-glutamine and 5% FCS (Gibco) and seeded in a 24-well cell culture plate at 5 x 105 cells/well ...
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bioRxiv - Developmental Biology 2023Quote: ... Optical density values were determined using a Multiskan™ FC Microplate Photometer (Thermo Scientific™ 51119000).
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 10% (v/v) fetal calf serum (FCS) (Gibco A5670401; Thermo Fisher Scientific, Waltham, MA) and 1% (v/v ...
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bioRxiv - Synthetic Biology 2024Quote: ... after which adsorption was measurement at 450 nm using a microplate reader (Multiscan FC, Thermo Scientific).
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bioRxiv - Microbiology 2024Quote: ... supplemented with 10 % (v/v) FCS and 0.5 % (v/v) Insulin-Transferrin-Selenium (Thermo Fisher Scientific). For the experiment ...
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bioRxiv - Immunology 2024Quote: ... prior to resuspension in complete RPMI (with 10% FCS, 1% penicillin/streptomycin, 1% L-glutamine; Gibco) and passing through a 70 µm cell strainer ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 10% fetal calf serum (FCS) (#S181BH-500, Biowest) and 1% penicillin-streptomycin (#15140122; Gibco). Purity > 97% of CD14+ monocytes after magnetic sorting was verified by flow cytometry (anti-CD14-FITC clone MφP9 ...
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bioRxiv - Biophysics 2021Quote: ... mCherry-tagged protein-expressing Neuro2a cells were incubated in a medium containing 0.5 μM Lysotrascker green DND-26 (Thermo Fisher) for 30 min before image acquisition.
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bioRxiv - Developmental Biology 2021Quote: Immunoprecipitations from HEK293T cells expressing either HA-or FLAG-tagged Yap were performed using the Dynabeads Protein A Immunoprecipitation Kit (Invitrogen) by coupling 5 μg of anti-FLAG (Cell signaling ...
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bioRxiv - Cell Biology 2020Quote: ... The Flp-In T-Rex HEK293 cells were transfected with the N-terminal GFP-tagged FAM83F packaged in a pcDNA5-FRT/TO vector along with Flp recombinase pOG44 (Invitrogen) in a ratio of 1 μg ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was then incubated with appropriate antibodies for 12 h and then conjugated with dynabeads Protein A or Protein G beads for an additional 1 h SFB- or GFP-tagged proteins were immunoprecipitated with streptavidin dynabeads (Invitrogen) or GFP-Trap (Chromotek) ...
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bioRxiv - Molecular Biology 2021Quote: ... cerevisiae EKY3/pYES2-TOP1 induced for expression of wild-type human TOP1with the PrepEase Histidine-Tagged Protein Purification Midi Kit – High Specificity (from Affymetrix) according to the manufacturer’s procedures ...
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bioRxiv - Molecular Biology 2020Quote: 6xHis-tagged REV-ERBβ LBD (4 nM) was incubated with anti-His antibody conjugated to terbium (1 nM) (ThermoFisher #PV5863) and FITC-labeled NCoR ID1 or NCoR ID2 peptide (400 nM ...
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bioRxiv - Molecular Biology 2020Quote: ... MBP-tagged full-length TRIM21 was cloned into a pFastBac vector for expression in the baculovirus system (Bac-to-Bac, Invitrogen), resulting in pFastBac-MBP-TRIM21 ...
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bioRxiv - Molecular Biology 2020Quote: ... Large-scale expression of Strep-tagged proteins for purification was conducted in Trichoplusia Ni High Five cells (Thermo Fischer Scientific) in SF-900 medium (Gibco). Cells at a density of 2×106 cells/ml were infected with 1.5% of amplified virus and incubated for 40 hours ...
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bioRxiv - Bioengineering 2022Quote: ... supernatants were harvested and filtered with a 0.22 µm membrane.The His-tagged proteins were purified with the HisPur Ni- NTA Resin (Thermo Fisher, 88222). After three columns of washing with 25 mM Imidazole (pH 7.4) ...
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bioRxiv - Molecular Biology 2019Quote: Expression constructs for repeat RNAs or GFP-tagged codon-optimized DPRs were transfected in HEK293 cells or U-2 OS cells with Lipofectamine 2000 (Invitrogen) or FuGENE HD (Promega) ...
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bioRxiv - Molecular Biology 2019Quote: ... with Strep and His epitope tags was amplified by PCR from pIEx10-LRIM1 (Povelones et al. 2009) with BamHI tagged primers (LRIM1-F, LRIM1-R) and subcloned into pJET1.2 (Thermo Scientific) before cloning into pslVg-BGH-attP ...
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bioRxiv - Molecular Biology 2019Quote: BGH was amplified by PCR from pslVg-GFP-BGH by PCR with BamHI and NotI tagged primers (BGH-F, BGH-R) and subcloned into pJET1.2 (Thermo Scientific). attP was amplified from pBCPB+ (Groth et al ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid encoding for FLAG-tagged TM or IM-TM were transfected into EXPI293F cells according to the manufacturer (Thermo Fisher), and incubated at 37°C ...
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bioRxiv - Neuroscience 2019Quote: ... and subsequently incubated in secondary antibody solution (AlexaFluor 488-tagged goat anti-rabbit Ab, 1:1000, ThermoFisher Scientific, A-11008) for 1 h at RT ...
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bioRxiv - Cell Biology 2019Quote: ... 1-2 μg His-tagged calcineurin was first bound to Ni-NTA-agarose or magnetic Dynabeads (Thermo Fisher Sci. USA) in base buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... mCherry-(backbone pLV-CMV-bc) and FLAG-His-tagged(backbone Pcdna3) p16INK4A constructs were created using gateway technology (Life Technologies). For cell cycle profiling ...
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bioRxiv - Molecular Biology 2019Quote: T7-tagged cDNA template was PCR amplified and subjected to in vitro transcription using a MEGAscript T7 kit (Applied Biosystems). Amplified cRNA was purified with an RNeasy Mini Kit (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were next incubated in biotinylated secondary antibody for 1 hour at room temperature followed by incubation in fluorophore tagged horseradish peroxidase (Alexa Fluor; Thermofisher). Sections were washed thrice in PBS-T and counterstained with RNAscope DAPI for 30 seconds ...
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bioRxiv - Biochemistry 2020Quote: ... pcDNA5/FRT/TO plasmid encoding Flag-tagged WT ExoU or S142A ExoU was incubated in serum free medium containing lipofectamine 2000 (Invitrogen) for 30 minutes at ambient temperature ...
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bioRxiv - Biochemistry 2020Quote: MBP-tagged (pTHMT) full-length SARS-CoV2 nucleocapsid protein was expressed in Escherichia coli BL21 Star (DE3) cells (Life Technologies). Bacterial cultures were grown to an optical density of 0.7 to 0.9 before induction with 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Bioengineering 2021Quote: ... supernatants were harvested and filtered with a 0.22 μm membrane.The His-tagged proteins were purified with the HisPur Ni-NTA Resin (Thermo Fisher, 88222). After three columns of washing with 25 mM Imidazole (pH 7.4) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were co-transfected with either a constant concentration of 0.1 μg of NF1-NanoLuc pcDNA expression plasmid or CRAF RBD-NanoLuc pcDNA expression plasmid with 0.2 μg of GFP-tagged KRAS (WT or Mutant) with 0.25 μl of Lipofectamine 2000 per well following the manufacturer’s protocol (Thermo Fisher Scientific). Twenty-four hours later ...
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bioRxiv - Cell Biology 2021Quote: ... full length N-terminally 3xFLAG tagged human KNSTRN ORF was cloned into pTREx-DEST30 vector by serial Gateway recombinations (Invitrogen) according to the manual ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transiently transfected with mCherry-tagged wild type or mutant ORP1L at 70% confluency using Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... The generation of HeLa stable cell lines expressing all different variants of FLAG-tagged HERPUD1 cloned in the pLVX-IRES-Puro vector were generated by transfection with Lipofectamine 2000 (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... Clarified lysates containing His6x-tagged proteins were subjected to gravity-flow Ni2+-affinity purification using HisPur Ni-NTA Resin (ThermoFisher) following manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2020Quote: ... LLC-luc cells were stably transfected with CIITA overexpression vector pcDNA3-myc-tagged CIITA (addgene Plasmid #14650) or control pcDNA3.1 (Thermofisher V79020) using Lipofectamine 2000 Reagent (Invitrogen 11668-027) ...