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Citations for Thermo Fisher :
2051 - 2100 of 7943 citations for Recombinant Human Leptin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Stable cell lines expressing BioID tagged genes of interest were generated by transfection with Lipofectamine 2000 (Thermo Fisher Scientific) and a combination of the appropriate pcDNA5/FRT/TO construct and Flipase expressing pOG44 plasmid ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids for expressing tagged proteins in S2 cells were generated by cloning coding sequences into pEntr/SD (Thermofisher, K242020), and recombining the coding sequences into pAc5 promoter destination vectors (pAWM and pAWF) ...
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bioRxiv - Cell Biology 2021Quote: ... and GST-tagged baits contained in supernatants were normalized using Pierce Glutathione Magnetic Agarose Beads (Thermo Fisher Scientific, #78602) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: IPs with FLAG-tagged protein were performed with Protein G magnetic beads (Invitrogen™ Dynabeads™ Protein G; #10004D) and α-FLAG antibody (Monoclonal ANTI-FLAG® M2 antibody produced in mouse ...
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bioRxiv - Cell Biology 2022Quote: ... Cells at 40% confluence were transfected with 1 ug of GFP-tagged LRRK2 plasmid using Lipofectamine 2000 (Invitrogen, 11668019) as described for 12-15 hrs ...
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bioRxiv - Neuroscience 2019Quote: ... Myc-tagged proteins were immunoprecipitated using anti-c-Myc monoclonal antibody supernatant (9E10, DSHB) and protein A-agarose (Invitrogen). Flag-tagged proteins were immunoprecipitated using anti-Flag-M2 affinity gel (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... HEK293T cells were transfected with N-terminally 3XFLAG and V5-tagged wild type mouse ZBP1 or Zα1α2-mutant mouse ZBP1 transducing vectors in the pLenti6 backbone (Life Technologies) (Maelfait et al. ...
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bioRxiv - Microbiology 2021Quote: ... were used to pull-down FLAG-tagged proteins and any associated proteins rather than the Dynabead Protein G (Invitrogen) pre-bound with anti-FLAG M2 antibody (SigmaAldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified A-C/EBP (30 - 40 μg) was tagged with Alexa Fluor 488 according to manufacturer’s protocol (Thermo fisher). Time lag expression of A-C/EBP protein was observed using EGFP tagged A-C/EBP plasmid (pCMV-A-C/EBP-EGFP ...
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bioRxiv - Immunology 2019Quote: ... The labeled cells were counter-stained with a fluorophore-tagged secondary goat anti-rabbit IgG (H+L) antibody (Invitrogen), before acquisition on a LSR Fortessa (BD Biosciences) ...
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bioRxiv - Biophysics 2020Quote: ... The C-terminally SBP-tagged DISP1-A or DISP1-A-NNN was captured using Streptavidin-agarose affinity resin (ThermoFisher). The eluted protein was concentrated by an Amicon Ultra filter (100 kDa cutoff ...
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bioRxiv - Developmental Biology 2021Quote: ... Each required tagged protein expression construct was transfected in HEK 293T cells with Lipofectamine 3000 transfection reagent (L3000150; Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... captured dsRNA was detected by incubating with sulfo-tagged rat anti-mouse IgM antibody (Thermo Fisher Scientific, Waltham, MA) for 1h before washing and adding MSD GOLD Read Buffer A (Meso Scale ...
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bioRxiv - Biophysics 2022Quote: ... stably expressing N-terminally GFP-tagged murine GBP2 (GFP-mGBP2) or GFP alone11 were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen/Gibco) supplemented with 10% (v/v ...
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bioRxiv - Biochemistry 2022Quote: A DNA fragment coding HA-tagged Siwi was amplified by PCR and cloned into pIZ vector (Thermo Fisher/Invitrogen) by In-fusion cloning kit (Takara) ...
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bioRxiv - Biochemistry 2022Quote: A DNA fragment coding HA-tagged Siwi was amplified by PCR and cloned into pIZ vector (Thermo Fisher/Invitrogen) by In-fusion cloning kit (Takara) ...
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bioRxiv - Microbiology 2022Quote: ... The cells were subsequently washed with TSB and incubated with WGA tagged with Alexa Fluor 488 (WGA-488, Invitrogen) at a final concentration of 2 μg/ml for 5 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... Transgenic S2 cells carrying the SIN3 187 transgene tagged with an HA epitope were grown in medium containing 0.1 mg/ml penicillin and streptomycin (Gibco). For treatment with CuSO4 ...
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bioRxiv - Plant Biology 2023Quote: ... Epitope-tagged proteins were detected by the HRP activity on SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher 34095) using a Gel Doc™ XR+ Gel Documentation System (Bio-Rad).
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bioRxiv - Plant Biology 2023Quote: ... Myc-tagged proteins were detected by the HRP activity on SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher 34095) using a Gel Doc™ XR+ Gel Documentation System (Bio-Rad).
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bioRxiv - Plant Biology 2023Quote: ... mYFP tagged proteins were detected by the HRP activity on SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher 34095) using a Gel Doc™ XR+ Gel Documentation System (Bio-Rad).
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bioRxiv - Molecular Biology 2022Quote: ... cells were transfected with uLimch1 and mLimch1 epitope tagged expression plasmids using Lipofectamine 3000 following the manufacturer’s protocol (catalog number L3000015, Invitrogen). mLimch1 and uLimch1 epitope tagged expression plasmids were commercially synthesized by GenScript ...
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bioRxiv - Neuroscience 2024Quote: ... samples were washed and incubated with donkey anti-mouse secondary antibody tagged with Alexa Fluor 647 (Thermo Fisher #A32787) diluted in fresh blocking solution ...
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bioRxiv - Cancer Biology 2024Quote: ... or Flag-tagged proteins were generated by inserting PCR-amplified fragments into the pcDNA3.1(+) mammalian expression vector (V79020, Invitrogen). Eukaryotic expression vectors encoding green fluorescent protein (GFP)-tagged proteins were generated by inserting PCR-amplified fragments into pd1-EGFP-N1 vector (6073-1 ...
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bioRxiv - Molecular Biology 2023Quote: ... the C-terminal HA-tagged Cas12f/sgRNA-encoding plasmids were transfected into HEK293T cells by using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... slides were washed twice with PBS and stained with AlexaFluor 488-tagged donkey-anti-goat (Molecular Probes, 1:100), Cy3-tagged donkey-anti-chicken (Jackson Immuno ...
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bioRxiv - Synthetic Biology 2023Quote: Tetra-cysteine (TC) tagged proteins (CCPGCC) were site-specifically labelled using the TC-ReAsH II TC detection dye (Invitrogen). TC-HERD-2.2–TnaA and TC-HERD-2.2–FMO (100 μM ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies were-donkey anti-rabbit or donkey anti-mouse tagged with either Alexaflour488 or Alexafluor568 (1:1000; Invitrogen) or goat anti-rat Alexaflour350 (1:200 ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were gently mixed with a 2x solution such that the final concentrations were 0.5mg/mL Alexa Fluor 647-tagged 10,000 MW dextran (Invitrogen #D22914), 200nM caged fMLP (NEP) ...
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bioRxiv - Immunology 2024Quote: ... Cells were transfected with C9-tagged SARS-CoV-2 S protein or with empty vector (pcDNA3.1) using Lipofectamine 2000 (Invitrogen) and maintained in antibiotic free DMEM supplemented with 10% FBS ...
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bioRxiv - Microbiology 2024Quote: Erythrocyte pull-down studies were performed with 6His-tagged EtpA linked to cobalt-coated magnetic beads (Dynabeads, Invitrogen 10103D) and blood group A1 red blood cells (RBC ...
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bioRxiv - Microbiology 2020Quote: ... DNA samples containing the extension products were added to Hi-Di formamide (Applied Biosystems, Foster City, CA). The mixture was incubated for 5 min at 95°C ...
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bioRxiv - Cell Biology 2020Quote: ... Air-liquid interface medium consisted of 50% BEGM and 50% hi-glucose minimal essential medium (DMEM, Gibco) containing 100 nM additional retinoic acid (Sigma Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... parahaemolyticus POR1 genomic DNA and inserted into the multiple cloning site (MCS) of pBAD/Myc-His (Invitrogen) vector containing a kanamycin-resistance cassette 25 (in-frame or out-of-frame with the C-terminal Myc-His tag ...
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bioRxiv - Genetics 2020Quote: ... The human PROS1 cDNA (GenBank NM_000313.4) was PCR-amplified from HepG2 cDNA and cloned into pcDNA3.1/myc-His(−) plasmid (Invitrogen). The PCR amplification of PROS1 from c.-44 to c.2028 was performed using Phusion High Fidelity (HF ...
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bioRxiv - Developmental Biology 2021Quote: ... The others were TA-cloned to pcDNA3.1/V5-His TOPO TA vectors (Cat. No. K4800-01, Invitrogen). HEK293T cells were cultured in DMEM medium with 10% FBS in 5% CO2 at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The araC gene and araBAD promoter were PCR-amplified from pBAD/His/lacZ (Invitrogen, Carlsbad, CA, USA). The lac promoter and inhibitory unit (iUnit ...
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bioRxiv - Developmental Biology 2020Quote: ... The template was prepared using the Ion PI Hi-Q OT2 200 kit (Thermo Fisher Scientific, UK). Sequencing was performed using the Ion PI Chip Kit v3 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... using the following primary/secondary antibodies: rabbit anti-6x-His Tag (ThermoFisher Scientific PA1-983B, 1:10,000), mouse monoclonal anti-GFP (Abcam ab1218 ...
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bioRxiv - Bioengineering 2019Quote: ... and cloned into the standard mammalian expression vector pEF1/myc-His B (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Immunology 2021Quote: RBD-His protein was expressed in Expi293 cells and purified on Ni-NTA resin (#88221, Thermo Fisher) followed by size-exclusion chromatography on a Superdex 200 gel filtration column in PBS.51 S1-S2-His (referred as S ...
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bioRxiv - Cell Biology 2020Quote: ... The protease was removed using His-Pur™ Ni-NTA agarose resin (Thermo Fisher Scientific, Waltham, MA). Gle1152-360 was further purified by a 30% ammonium sulfate precipitation ...
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bioRxiv - Cell Biology 2021Quote: ... 6xHis-SNAP-Rab5B was then captured onto 20 μL Dynabeads His-Tag Magnetic Beads (Thermo Fisher Scientific) and washed three times in Ni-binding buffer in 2 mL Protein Lo Bind Tubes (Eppendorf) ...
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bioRxiv - Bioengineering 2020Quote: ... HEK 293T cells were cultured in DMEM (HyClone) supplemented with 10% heat-inactivated FBS (HI-FBS; ThermoFisher). CHLA-255-Luc-EGFP cells were cultured in IMDM (ThermoFisher ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The stable cell lines were generated using the pcDNA/FRT/V5-His TOPO TA Expression kit (Invitrogen) performed according to the manufacturer’s protocol and as described previously (28) ...
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bioRxiv - Microbiology 2021Quote: ... large-scale expression cultures were performed with Hi-5 cells cultured in Express-Five media (Life Technologies) and infected with 0,5% vol/vol of generation 2 (V1 ...
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bioRxiv - Neuroscience 2020Quote: ... CCM2 isoforms were inserted into pcDNA3.1/V5-His-TOPO and Topo pCR®II plasmid vectors (Invitrogen) respectively (1-6 ...
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bioRxiv - Molecular Biology 2020Quote: The sequence of the OpIE2 promoter was obtained from pIB/v5-His map sequence (ThermoFisher Scientific, USA). The truncated version of the wild-type OpIE2 promoter sequence (tIE2) ...
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bioRxiv - Microbiology 2020Quote: ... The templates were sequenced with an Ion PGM Hi-Q View Sequencing Kit (Thermo Fisher Scientific Inc.) and a 318 Chip Kit v2 (Thermo Fisher Scientific Inc. ...
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bioRxiv - Biochemistry 2022Quote: ... Ribosome nascent chain complexes (RNCs) were purified via the encoded His-Tag using magnetic Dynabeads (Invitrogen, #10104D). The translation reaction was applied to equilibrated beads (50 mM HEPES pH 7.5 ...