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Citations for Thermo Fisher :
2051 - 2100 of 4797 citations for Placental lactogen Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Fixed HUVEC were blocked for 1h at RT in blocking solution (5% FBS, 2X antibiotic-antimycotic (Gibco), 0.1% sodium azide (s2002-100G ...
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bioRxiv - Neuroscience 2019Quote: ... We replaced the blocking solution with primary antibody solution (1:500 anti-GFP-rabbit IgG; Life Technologies) for 12 hours at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... and incubated for 1 hr in blocking buffer (10% Normal Goat Serum; Thermo Fisher Scientific; Waltham, MA) containing 0.3% Triton X-100 ...
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bioRxiv - Cell Biology 2019Quote: ... Blocking was performed for 30 min at RT and incubation with rabbit anti-human AlexaFluor 647 (Invitrogen) that binds to the Tmab Fc region of T-DM1 and Accum-T-DM1 ...
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bioRxiv - Cell Biology 2019Quote: ... The membranes were washed four times with blocking buffer before adding HRP-conjugated secondary antibodies (Life Technologies), diluted as recommended in blocking buffer ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked in Starting Block Tris-buffered saline (TBS) blocking buffer (Thermo-Fisher Scientific, Darmstadt, Germany) for 1h and incubated with antibodies specific for p38 (Cell Signaling Technology ...
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bioRxiv - Microbiology 2021Quote: ... Non-specific binding to the membrane was blocked with Odyssey® PBS Blocking Buffer (Fisher Scientific NC9877369) and probed with the antibodies listed below ...
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bioRxiv - Cell Biology 2020Quote: ... The subsequently permeabilised cells were washed twice with PBS before blocking in 2% Goat serum (Life Technologies) and 1% BSA (Sigma).
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bioRxiv - Pathology 2021Quote: ... Membranes were blocked for 1 hour at RT using Pierce™ Clear Milk Blocking Buffer (ThermoFisher Scientific). Blots were incubated overnight at 4°C with mouse anti-FLAG tag mAB (Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2020Quote: ... the vitelline membrane was carefully removed and embryos were transferred to CAS blocking reagent (Invitrogen, cat. # 008120). For staining of sections ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained as follows: 1-blocking with 2 μg/mL FcBlock (anti mouse CD16/CD32, Invitrogen) for 1 h at RT in microscopy buffer (5% w/V BSA (Sigma) ...
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bioRxiv - Developmental Biology 2020Quote: ... we incubated samples in BODIPY® FL Phallacidin (1:40 dilution in blocking buffer, Thermo Fisher Scientific) at RT for 1 hour ...
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bioRxiv - Physiology 2021Quote: ... Sections were blocked with StartingBlock™ T20/phosphate buffered saline (PBS) Blocking Buffer (Thermo Scientific, Waltham, MA) before being incubated with primary antibodies (in 0.2% Triton X-100 ...
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bioRxiv - Immunology 2021Quote: ... The tissue was washed again as described before and incubated with blocking buffer-diluted Bodipy (Thermofisher # D3922) for 1h at room temp ...
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bioRxiv - Genomics 2021Quote: ... the samples were bathed in blocking buffer for 15 min before being stained by DRAQ5TM (Thermo Fisher) for 10 min ...
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bioRxiv - Cell Biology 2021Quote: ... Cross-absorbed secondary antibodies tagged with Alexa-fluorophores were diluted in blocking buffer (1:400, Invitrogen, Switzerland) and applied for 30 min at RT ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by incubation with secondary antibodies in blocking buffer and 1 μg/ml DAPI (Thermo Fisher Scientific) in blocking solution for 50 min at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... and blocked for 45 minutes using a blocking buffer containing DPBS with 5% goat serum (ThermoFisher #16210064) 0.05% sodium azide (Sigma #S2002 ...
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bioRxiv - Cell Biology 2022Quote: ... and washed 3 times with blocking buffer before mounting on glass slides with Prolong Gold (ThermoFisher Scientific). For immunoblotting ...
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bioRxiv - Cell Biology 2022Quote: ... then incubated with blocking buffer (10% normal goat serum–50062Z, Thermo Fisher; 0.5% BSA–BP1600, Thermo Fisher; 0.2% Triton X-100-T8787 ...
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bioRxiv - Immunology 2023Quote: ... 1% OVA in PBST for the second round and ELISA Blocker Blocking Buffer (Thermo Fisher Scientific, N502) for the third round ...
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bioRxiv - Microbiology 2023Quote: ... followed by destaining using 25 mM NaOH and blocking using 1x BlockerTM FL Fluorescent protein block (ThermoFisher). Primary antibodies used were Anti-GapDH (G9545 ...
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bioRxiv - Developmental Biology 2023Quote: ... oocytes were incubated with the secondary antibodies (1:500 in blocking solution, Alexa Fluor, Thermo Fisher Scientific) for 1 hour in dark ...
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bioRxiv - Neuroscience 2023Quote: ... Sections stained with mouse primary antibodies were blocked in ReadyProbes “Mouse on Mouse” IgG blocking solution (Thermofisher) for 1 hour before the initial blocking step ...
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bioRxiv - Immunology 2023Quote: ... was used at 1:8000 dilution in blocking solution together with 0.01 mg/ml DAPI (Thermo Fisher). The wells were washed with wash buffer and PBS ...
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bioRxiv - Genetics 2023Quote: ... 0.08% blocking reagent) containing 10 µL H2O2 0.3% and 2.5 µL of Alexa488 fluorochrome-labeled tyramides (Invitrogen). Sections were washed two times in PBS for 10 min at RT ...
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Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat productionbioRxiv - Bioengineering 2023Quote: ... and blocked for 45 minutes using a blocking buffer of DPBS with 5% goat serum (ThermoFisher #16210064) and 0.05% sodium azide (Sigma #S2002) ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were diluted in 7.5ml Intercept PBS blocking buffer with 0.1% Tween (SynGAP1; 1:1000dil, Invitrogen, PA1-046 ...
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bioRxiv - Neuroscience 2023Quote: ... incubated with Alexa 647 labeled donkey anti-rabbit secondary antibodies (1:250 in blocking solution; Invitrogen A32795TR) for 1 h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... A blocking solution was then added with 0.1% Triton-X-100 and 3% bovine serum albumin (Invitrogen). An antibody solution of MAP2 (1:2500 ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was blocked for 1 h at RT with nucleic acid detection blocking buffer (Life Technologies), then probed for 1 h at RT with streptavidin-HRP 1:5,000 (Genescript ...
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bioRxiv - Developmental Biology 2023Quote: ... and incubated in blocking solution with goat or donkey raised Alexa Fluor-conjugated secondary antibodies (Thermo Fisher), at room temperature for 24 hours ...
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bioRxiv - Pathology 2024Quote: ... HT7 was the only anti-mouse antibody used and a mouse-on-mouse blocking agent (Invitrogen: R37621) was applied for 30 minutes post protein block washes to reduce noise from secondary antibody binding and an additional three 5-minute washes were added before primary antibody incubation ...
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bioRxiv - Neuroscience 2024Quote: ... BV-2 cells were fixed and sequentially incubated with Mouse-on-Mouse IgG Blocking Solution (Invitrogen R37621) and Navenci blocking solution at 37°C for 60 min each in a pre-heated humidified chamber ...
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bioRxiv - Bioengineering 2024Quote: ... for 1 hour before blocking with 3% (w/v) bovine serum albumin (BSA, Fisher Scientific, Cat # BP9703100). To detect ARP3 and GLUT4 ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were blocked with 1X BlockerTM FL 10X Fluorescent blocking buffer (Thermo Fisher Scientific, Cat # 37565) followed by incubation with the respective primary antibodies overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... The fixed slides were washed with PBS for twice and blocked with Blocking buffer (Thermo Fisher Scientific) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Peptides were loaded onto the trapping column (PepMap100, C18, 300 µm x 5 mm, Thermo Scientific) using partial loop injection with 2% acetonitrile (ACN) ...
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bioRxiv - Cancer Biology 2021Quote: ... fractionated into 8 fractions using the High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific 84868) according to the manufacturer protocol and dried ...
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bioRxiv - Cancer Biology 2021Quote: Peptide mixtures were separated by reversed-phase chromatography on an EASY-Spray column (Thermo Fisher Scientic), 25-cm long (inner diameter 75 μm ...
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bioRxiv - Biochemistry 2021Quote: ... with 15 µM Impβ mixed with 75 µM Rev-peptide and 1x SYPRO Orange (Molecular Probes) diluted in H2O ...
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bioRxiv - Cell Biology 2020Quote: ... Cleaved peptides were analyzed by nanoflow LC-ESI-MS/MS (Thermo Scientific LTQ Orbitrap Velos Pro). Peptide sequences were mapped using ProteinProspector and Mascot software packages.
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bioRxiv - Cell Biology 2020Quote: ... The isobaric labelling of the peptides was performed using 10-plex TMT reagents (Thermo Fisher Scientific).
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REEP4 is recruited to the inner nuclear membrane by ELYS and promotes nuclear pore complex formationbioRxiv - Cell Biology 2020Quote: Peptides were analyzed by C18 nanoflow reversed-phase HPLC (Dionex Ultimate 3000 RSLC nano, Thermo Scientific) combined with orbitrap mass spectrometer (Q Exactive HF Orbitrap ...
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bioRxiv - Microbiology 2021Quote: ... Peptides were loaded with the autosampler directly onto a 50 cm EASY-Spray C18 column (ThermoFisher). Peptides were eluted from the column using a Dionex Ultimate 3000 Nano LC system with a 14.8 min gradient from 1% Buffer B to 23 % Buffer B (100% acetonitrile ...
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bioRxiv - Developmental Biology 2020Quote: ... desalting and fractionation was performed with Pierce High pH Reversed-Phase Peptide Fractionation Kits (Thermo Scientific) to generate 8 fractions per sample ...
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bioRxiv - Developmental Biology 2022Quote: ... Peptide desalting was performed according to the manufacturer’s instructions (Pierce C18 Tips, Thermo Scientific, Waltham, MA). Desalted peptides were reconstituted in 0.1% formic acid in water and further separated into four fractions by strong cation exchange chromatography (SCX ...
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bioRxiv - Molecular Biology 2020Quote: ... Peptide desalting was performed according to the manufacturer’s instructions (Pierce C18 Tips, Thermo Scientific, Waltham, MA). Eluates were lyophilized and reconstituted in 11 µL of 5% acetonitrile and 2% formic acid in water ...
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bioRxiv - Microbiology 2021Quote: Peptides were labelled with Alexa Fluor® 488 (A488) 5-sulfodichlorophenol ester (SDP) (Thermo Fisher Scientific) using previously described methods [66] ...
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bioRxiv - Immunology 2021Quote: ... The digested peptides were labelled with iTRAQ reagents according to the manufacturer’s protocols (Applied Biosystems, USA). The peptides from the Hemocyte samples collected at four different time points were labelled with four 8-plex iTRAQ reagents (Applied Biosystems ...