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Citations for Thermo Fisher :
2051 - 2100 of 10000+ citations for Mouse OLFR139 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... purified and cloned in the plasmids pCR8/GW/TOPO® (Invitrogen) using the primers listed in Supplementary Table S2 ...
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bioRxiv - Biochemistry 2022Quote: ... U2OS cells were transfected with plasmid DNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions and analysed 24 hours after transfection ...
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bioRxiv - Microbiology 2022Quote: ... PCR and cloned into the TOPO-TA plasmid (Thermo Fisher Scientific). The human wild-type GBA1-containing TOPO-TA plasmid was then used as a template to generate the N370S and L444P GBA1 variants ...
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bioRxiv - Neuroscience 2022Quote: ... or pCINeo (transfection control plasmid) using Lipofectamine 2000 reagent (Thermo Scientific). Cells were fixed using 4% paraformaldehyde (PFA ...
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bioRxiv - Molecular Biology 2023Quote: ... the plasmid was digested with BsmBI (FastDigest Esp3I, Thermo Fisher Scientific) and a synthetic 391 bp double-stranded DNA fragment encoding 5′-(1st gRNA/scaffold/H1 promoter/2nd gRNA)-3′ was inserted using the NEBuilder HiFi assembly system (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were cut using appropriate restriction enzymes (Fast Digest-Thermo Scientific), dephosphorylated with FastAP and purified from an agarose gel with a Zymoclean Gel DNA Recovery kit (Zymo Research) ...
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bioRxiv - Microbiology 2023Quote: ... pyciF was purified (GeneJET plasmid miniprep kit, Thermo Fisher Scientific, Lithuania) and confirmed by restriction digestion to visualize the insert release and Sanger sequencing (Aggrigenome ...
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bioRxiv - Immunology 2023Quote: HeLa cells were transfected with receptor plasmids using Lipofectamine 3000 (Invitrogen) for 24 hrs ...
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bioRxiv - Developmental Biology 2024Quote: ... Plasmid DNA was isolated using a midi-prep kit (Invitrogen; K210005). The integrity of the CRISPRi library was validated by Illumina Miseq through Cornell Genomics Facility (detailed library preparation steps are presented below in “Library validation and CRISPRi dropout screening”).
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bioRxiv - Molecular Biology 2022Quote: ... The expression kit containing pPICZαB plasmid was purchased from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were transfected with pCBA-SceI plasmid using Lipofectamine 2000 (Invitrogen). Cells were harvested 48 hrs post-transfection ...
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bioRxiv - Molecular Biology 2022Quote: ... and 500 ng TORNADO Broccoli expression plasmid using Lipofectamine 3000 (Invitrogen). After 48 and 72 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmid transfection was performed using Lipofectamine 3000 Reagent (Thermo Fisher Scientific), in accordance with the manufacturer’s instructions.
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bioRxiv - Biophysics 2022Quote: ... the vector backbone was amplified from the pcDNA5-FRT plasmid (Invitrogen), the stdMCP-stdGFP fragment was amplified from the pUbC-NLS-HA-stdMCP-stdGFP plasmid (Addgene ...
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bioRxiv - Cell Biology 2022Quote: Plasmid DNA was electroporated into iPSDMs using the Neon system (Invitrogen). iPSDM were resuspended at 1.5×106 cells in 100 µl buffer R ...
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bioRxiv - Bioengineering 2024Quote: ... and the YAP/TAZ-TRE-tdTomato plasmid using Lipofectamine 2000 (Invitrogen). Cells were cultured for 2 days post-transfection ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293FTs were transfected with plasmid DNA using lipofectamine 3000 (Invitrogen, L3000015) at 70-80% confluency ...
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bioRxiv - Microbiology 2024Quote: ... Individual reporter plasmids were amplified by EquiPhi29 polymerase (Thermo Fisher Scientific) and transformed into recipient B ...
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bioRxiv - Cell Biology 2024Quote: ... and the aforementioned plasmid in Opti-MEM medium (Thermo Fisher Scientific). This mixture was allowed to interact at RT for 5 minutes prior to application ...
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bioRxiv - Neuroscience 2024Quote: ... Plasmid DNA was linearized using the Mlu1 restriction enzyme (Thermo Fisher) and then in vitro transcribed using the Sp6 RNA polymerase (Thermo Fisher) ...
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bioRxiv - Microbiology 2024Quote: ... and ligated into pBAD/Thio-TOPO plasmid vector (Thermo Fisher Scientific). The plasmids were transformed into TOP10 competent cells (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... The FLAG-pTBK1-HA plasmid was generated by Gateway cloning (Invitrogen), using the FLAG-pTBK1 as template for generation of the entry clone (forward primer ...
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bioRxiv - Cell Biology 2024Quote: ... plasmids were transfected into the cells using Lipofectamine2000 (Life Technologies 11668019). 1 µg of DNA was complexed with 3 µg of Lipofectamine2000 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with plasmid DNA using Lipofectamine 2000 (Thermo Scientific) for approximately 48 h ...
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bioRxiv - Genomics 2024Quote: ... and packaging plasmids psPAX2 and pMD2.G using Lipofectamine 3000 (Invitrogen). Virus- containing medium was collected 48 and 72 h post transfection ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmid transfection was performed using Lipofectamine 2000 or Lipofectamine 3000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... about 250-300 ng of plasmids were transfected using Fugene (Invitrogen). p53-TA-Luc (Clontech/Takara ...
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bioRxiv - Cell Biology 2024Quote: Plasmids were constructed using Gateway assembly (Thermo Fisher Scientific, Waltham, MA). The pME’ clrn1 entry clone was generated by amplifying the full-length cDNA of clrn1 (ENSDART00000171202.2 ...
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bioRxiv - Immunology 2024Quote: ... and plasmid DNA was isolated using a Maxiprep kit (Invitrogen, K210016). RRV production ...
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bioRxiv - Microbiology 2024Quote: ... All vectors were purified using the GeneJet Plasmid Kit (Thermo Scientific) and sequence verified by Sanger sequencing (PSOMAGEN) ...
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bioRxiv - Molecular Biology 2024Quote: ... Amplified DNA fragments were cloned into pENTR/D-TOPO plasmids (Invitrogen) as recommended by the manufacturer ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with appropriate plasmids using Lipofectamine 3000 (Thermo Fisher) by reverse transfection as previously described (30) ...
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bioRxiv - Systems Biology 2023Quote: ... One μg of sgRNA plasmids were transfected with Lipofectamine 3000 (Invitrogen) 1 μg of pPAX2 and 0.1 μg VSVG into HEK293Ts seeded the night before at 2e5 cells per 6 well ...
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bioRxiv - Developmental Biology 2023Quote: ... 4 μg of the plasmid was transfected using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... and cloned into the pENT-D-Topo plasmid (Thermofisher, Rochester,U.S.A). The entry vectors were fully sequenced to ascertain the integrity of the cloned sequences and were used to transfer the branching enzyme sequences to the ChampionTM pET300 plasmid (Thermofisher ...
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bioRxiv - Microbiology 2023Quote: ... which were purified with GeneJET Plasmid Midiprep kit (Thermo Scientific, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were transfected with plasmid DNA using Lipofectamine 2000 (ThermoFisher #11668019) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... and transfer plasmid pLenti-NLuc1-9_IRES_EGFP (Figure S5) using Lipofectamine2000 (Invitrogen). DNA ratio of plasmid mix was 2:1:4 ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were purified using a maxiprep kit (Thermo Fisher Scientific, A31217).
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bioRxiv - Cell Biology 2023Quote: Plasmid DNA (1-2 μg) was transiently transfected with Optimem (Gibco) and Lipofectamine 2000 (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... plasmids at a 49:1 ratio using Lipofectamine 2000 (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Plasmids were transfected using TurboFect transfection reagent (ThermoFisher Scientific, Basel, Switzerland) and siRNAs using Lipofectamine RNAiMax (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... modified pFastBac1 plasmids was performed using T4 DNA Ligase (Thermo Scientific). Ligated plasmids were amplified in E ...
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bioRxiv - Neuroscience 2023Quote: ... The plasmid was used to transfect Expi293F cells (Thermo Fisher, USA) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... 1 µg plasmid DNA was diluted in 100 µl OptiMEM (ThermoFisher). The DNA mix was incubated with 100 µl OptiMEM containing 3 µl Lipofectamine2000 ...
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bioRxiv - Microbiology 2023Quote: ... 2 µg plasmid DNA was diluted in 100 µl OptiMEM (ThermoFisher). The DNA mix was incubated with 100 µl OptiMEM containing 6 µl polyethylenimine (PEI ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with the respective plasmids using Lipofectamine 2000 (Invitrogen) following the instructions provided by the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... plasmids at a 49:1 ratio using Lipofectamine 2000 (ThermoFisher Scientific) and read out as described above 24 h later.
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bioRxiv - Neuroscience 2023Quote: ... and the indicated plasmids were co-transfected using Lipofectamine 3000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: Plasmids were mainly constructed based on the Gateway technology (Life technologies). Primers used in the plasmid construction are listed in Table S1 ...