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Citations for Thermo Fisher :
2051 - 2100 of 10000+ citations for Mouse Anti Hepatitis B Surface Antigen Antibody 1838 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... antibodies were used for surface staining after LIVE/DEAD™ Fixable Aqua Dead Cell staining (ThermoFisher scientific). Briefly ...
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bioRxiv - Genomics 2024Quote: ... Antibodies were added to stain surface markers for 30 min at 4°C (CD3 PE, Life Technologies; CD8 FITC ...
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bioRxiv - Neuroscience 2024Quote: ... before coating the surfaces with a laminin solution consisting of 16 µg/mL natural mouse laminin (Gibco™, 23017015) diluted in phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2021Quote: ... The antibody-antigen complex was captured by incubating with lysis buffer prewashed proteinA/G beads (Thermo Fisher Scientific) for 2 h ...
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bioRxiv - Biochemistry 2020Quote: ... The antigen-antibody complexes were detected using the Pierce ECL chemi-luminescent detection system (Thermo Scientific, Rockford, IL) and visualized on the ChemiDoc XRS+ gel documentation system (Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: ... The antigen/antibody complex was eluted from beads by boiling in Lane Marker Sample Buffer (#39001, Thermo Fisher) containing β-mercaptoethanol for SDS-PAGE and Western blotting.
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bioRxiv - Developmental Biology 2022Quote: ... after dewaxing and antigen retrieval non-specific antibody binding was minimised by incubating sections in CAS Block (Invitrogen). Primary antibodies were diluted in Dako antibody diluent (S0809 ...
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bioRxiv - Plant Biology 2022Quote: ... Chemiluminescence detection of antigen-antibody complexes was carried out with SuperSignal West Femto Western substrate (Thermo Fisher Scientific). As a loading control ...
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bioRxiv - Immunology 2020Quote: ... Each mouse received 50 μg of antigen in 150 μL of PBS (NP40‑FICOLL) or PBS/Alum (77161, Thermo Fisher) adjuvant (2:1 ratio ...
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bioRxiv - Cell Biology 2020Quote: ... for one hour at 25°C and incubated overnight with the rabbit anti-tau (C-terminal) antibody (MilliporeSigma) or the mouse anti-phospho-Tau (Thr181) Clone AT270 antibody (Thermo Fisher Scientific, Waltham, MA, USA) at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... the primary antibodies were detected using HRP-conjugated secondary antibodies (goat anti-rabbit#65-6120 and goat anti-mouse-#65-6520; Invitrogen; dilution of 1: 5,000-10,000) following which the blots were developed using enhanced chemiluminescence substrate (Thermo Fisher-#34579).
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bioRxiv - Cancer Biology 2021Quote: ... Detection of all other primary antibodies was performed using a goat anti-mouse Poly HRP secondary antibody or goat anti-rabbit Poly HRP secondary antibody (Invitrogen B40961/2; 10-minute incubation). The HRP-conjugated secondary antibody polymer was detected using fluorescent tyramide signal amplification using Opal dyes 520 ...
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bioRxiv - Physiology 2023Quote: ... Membranes were then incubated with the primary antibodies (listed in Table 2) and HRP-linked secondary antibodies (anti-mouse or anti-rabbit IgG; Thermo Fisher Scientific, 10368172 and 11034). Clarity Western ECL (Bio-Rad ...
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bioRxiv - Genomics 2022Quote: B-cell subpopulations from PBMCs were stained with the monoclonal antibody anti-CD19(PE) (Clone HIB19; Invitrogen; Cat. num. 12-0199-41) in a 1:20 dilution for 30 minutes on ice ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies were Alexa Fluor-488 and -594 goat anti-rabbit and goat anti-mouse IgGs (1:200; Invitrogen). Imaging was performed on a Zeiss LSM880 + Airyscan Inverted confocal microscope using a 40x oil objective (numerical aperture ...
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bioRxiv - Cell Biology 2020Quote: ... ProteinTech) and secondary antibodies (Goat-anti-Mouse-Alexa-488, Thermo Fisher Scientific; Goat-anti-Rabbit-Cy5, Thermo Fisher Scientific) were diluted 1:1000 in PBST + 10% NGS ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies (Alexa Fluor 488, 594-conjugated goat anti-rabbit or anti-mouse immunoglobulins G (IgGs) (1/1,000; Invitrogen) at 37°C for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... Anti-mouse and anti-rat antibody conjugated to Alexa Fluor 488 or Alexa Fluor 546 (1:100, Life Technologies, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... the anti-mouse and anti-rabbit secondary antibodies conjugated with AlexaFluor 488 or AlexaFluor 594 (Invitrogen, Carlsbad, CA, USA) (diluted 1:1000 ...
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bioRxiv - Developmental Biology 2020Quote: ... Secondary antibodies were goat-anti-mouse Alexa Fluor 568nm and goat-anti-rabbit Alexa Fluor 488 (1:1000, Invitrogen).
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bioRxiv - Cell Biology 2022Quote: ... Lysates were centrifuged at 14,000 RPM for 30 seconds and separated on 8% SDS-PAGE gels followed by western blotting with mouse anti-HA (H9658, Clone HA-7, MilliporeSigma) or anti-PGK1 monoclonal antibodies (AB_2532235, 22C5D8, Invitrogen), and secondary polyclonal goat anti-mouse antibodies conjugated to horseradish peroxidase (115–035-146 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with Alexa 555-conjugated anti-mouse IgG1 and Alexa 488-conjugated anti-rabbit secondary antibodies (ThermoFisher), as appropriate ...
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bioRxiv - Biophysics 2019Quote: ... and Alexa Fluor 594-goat anti-rabbit and 633-donkey anti-mouse conjugated secondary antibodies (Invitrogen; 1:1000 dilution).
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bioRxiv - Microbiology 2020Quote: ... The membranes were washed with TBST and further reacted either with alkaline phosphatase-conjugated anti-mouse or anti-rabbit IgG secondary antibody (1:2000) or with horse radish peroxidase-conjugated anti-mouse or anti-rabbit IgG antisera (1:20,000) (Invitrogen) at room temperature for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... cells were stained with secondary antibody (goat anti-rabbit-AlexaFluor 488 or goat anti-mouse-AlexaFluor 488, Thermo Fisher) diluted in blocking buffer containing 1 μg/ml DAPI for 30 min at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... slides were incubated with Alexa 555-conjugated anti-rat and Alexa 488-conjugated anti-mouse secondary antibodies (Molecular Probes). DNA fibres were imaged in a LSM780 confocal microscope using a 64x oil objective and lengths of CldU and IdU tracks were measured using ImageJ software (http://fiji.sc/Fiji).
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bioRxiv - Neuroscience 2020Quote: ... secondary antibodies were applied (Goat anti-mouse Alexa 488, Abcam Cat# ab150113-500ug, Goat anti-rabbit Alexa 568, ThermoFisher Cat# A11036 ...
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CRISPR screens for lipid regulators reveal a role for ER-bound SNX13 in lysosomal cholesterol exportbioRxiv - Cell Biology 2021Quote: ... then permeabilized in 0.1% Saponin and stained with PFO*-Alexa647 or anti-BMP and Alexa Fluor 647 goat anti–mouse antibodies (Invitrogen). Quantification of cholesterol and BMP total fluorescence was performed using a BD Accuri C6 flow cytometer and data were analyzed using Flowjo software (Tree Star).
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bioRxiv - Neuroscience 2020Quote: ... followed by incubation with donkey Alexa Fluor 555-conjugated anti-mouse or anti-rabbit IgG antibodies (Thermo Fisher Scientific). Fluorescence images were acquired with a confocal laserscanning microscope (TCS SP8 ...
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bioRxiv - Developmental Biology 2021Quote: ... Anti-rat or isotype-specific anti-mouse secondary antibodies conjugated to Alexa Fluor-488 or - 568 (Thermo Fisher Scientific) were used at 1:400 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies were Alexa Fluor 546-conjugated goat anti-rabbit IgG or goat anti-mouse IgG (1:1,000; Invitrogen), or Alexa Fluor 488-conjugated goat anti-rabbit IgG or goat anti-mouse IgG (1:300 ...
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bioRxiv - Biochemistry 2020Quote: ... Secondary antibodies conjugated with horseradish peroxidase (anti-mouse IgG; anti-rabbit IgG, 1:2000 for western blot; Thermo Scientific). Function-blocking TREM2 antibody (Clone 78.18 ...
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bioRxiv - Microbiology 2021Quote: ... HRP-conjugated anti-Rabbit Ig and anti-mouse Ig antibodies (Cat#:NA931V and NA934V) were obtained from Thermo Fisher.
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bioRxiv - Microbiology 2021Quote: ... Cells were stained with Alexa Fluor 488 goat anti-rabbit and 594 goat anti-mouse secondary antibodies (Life Technologies). Cell nuclei were stained with DAPI in Prolong anti-fade mountant (Thermofisher scientific) ...
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bioRxiv - Microbiology 2020Quote: ... and secondary antibodies (Alexa488- and Alexa 594-conjugated anti-rabbit and anti-mouse, 1:100, ThermoFisher Scientific, Massachusetts, USA) diluted in PBS (1h ...
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bioRxiv - Cell Biology 2019Quote: ... primary antibodies and with Alexa Fluor 488 anti-rabbit and Alexa Fluor 594 anti-mouse (1:500, Life Technologies) secondary antibodies ...
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bioRxiv - Cell Biology 2019Quote: ... and the anti-mouse and anti-rabbit conjugated with Alexa Fluor 488 antibodies were from Invitrogen (Carlsbad, CA, USA). TRITC conjugated phalloidin was purchased from Cytoskeleton (Denver ...
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bioRxiv - Immunology 2019Quote: ... conjugated rabbit anti-mouse (1:64) or AlexaFluor 594-conjugated rabbit anti-donkey antibody (1:250; Invitrogen, Paisley, UK). All secondary antibodies had been tested and found to be negative for cross-reactivity against human epithelial cells ...
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bioRxiv - Neuroscience 2019Quote: ... Secondary antibodies used were Alexa-488 goat anti-mouse IgG and TexasRed goat anti-rabbit IgG (both from ThermoFisher). Controls were included in each run where the primary antibodies were omitted and these sections were used to set the background threshold prior to image capture ...
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bioRxiv - Neuroscience 2021Quote: ... but after rinsing sections were incubated in secondary antibodies (donkey anti-guinea pig 555 1:1,000 Sigma SAB4600298; goat anti-mouse 546 1:400 Invitrogen A-11030 ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated with an Alexa Fluor-568 conjugated goat anti-rabbit or anti-mouse antibody (Thermo Fisher Scientific) for 1 h ...
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bioRxiv - Microbiology 2019Quote: ... Secondary antibodies included Alexa Fluor 594 or Alexa Fluor 488 conjugated goat anti-rabbit and goat anti-mouse (Invitrogen), all used at 1:2,000.
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bioRxiv - Microbiology 2021Quote: ... Secondary antibodies were goat anti-rat AF647 and anti-mouse AF488 (diluted 1:1000, ThermoFisher #A-21247 #A-28175). The pan-flavivirus anti-Env 4G2 antibody was a kind gift from Phillipe Desprès.
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bioRxiv - Cancer Biology 2020Quote: ... Secondary antibodies: Alexa Fluor 568 donkey anti mouse IgG and Alexa Fluor 488 goat anti rabbit IgG (Molecular Probes). Cell nuclei were counterstained with 1 μg/ml DAPI (4’,6’-diamidino-2-phenylindole).
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bioRxiv - Biophysics 2022Quote: ... Secondary antibodies [Alexa 647-goat anti-mouse or Alexa 647 Goat anti-Human IgG (H+L) Cross-Adsorbed (Invitrogen)] at 1:1000 dilution were then incubated for 60 minutes at RT ...
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bioRxiv - Microbiology 2022Quote: ... Secondary antibodies included Alexa Fluor 594- or Alexa Fluor 488-conjugated goat anti-rabbit and goat anti-mouse (Invitrogen), all used at 1:1,000.
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bioRxiv - Physiology 2023Quote: ... incubated in appropriate secondary antibodies (Anti-rabbit IgG, 7074, Cell Signaling Technology; or anti-mouse IgG, 31450, Thermo Scientific), and washed in TBST ...
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bioRxiv - Developmental Biology 2024Quote: ... they were rinsed twice in 1X PBS and incubated with 1:1,000 dilutions of Alexa Fluor-conjugated donkey anti-mouse or donkey anti-rat secondary antibodies (Invitrogen) for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... For protein detection secondary anti-mouse or anti-rabbit antibodies conjugated either to Alexa Fluor 488 and 555 (Invitrogen) for immunofluorescence or to horseradish peroxidase (HRP ...
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bioRxiv - Developmental Biology 2024Quote: ... Secondary antibodies used included HRP-conjugated sheep anti-mouse (Amersham) and HRP-conjugated goat anti-rat (Thermo Fisher Scientific). For densitometry analysis of immunoblots ...