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Citations for Thermo Fisher :
2051 - 2100 of 10000+ citations for Mouse Activation Induced Cytidine Deaminase AICDA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: For competitive ELISA recombinant hACE2 protein were immobilized (400 ng per well) on immunoplates (Nunc MaxiSorp, Thermo Fisher Scientific, USA). RBD-specific nanobodies were mixed with HRP-conjugated recombinant RBD protein (0.2 μg/ml ...
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bioRxiv - Cancer Biology 2020Quote: Medium samples from cells and from the engrafted scaffolds were used to measure TGFβ using ELISA (88-8350-22, ThermoFisher), following manufacturer’s guidelines ...
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bioRxiv - Immunology 2020Quote: ... submandibular bleeding was performed to collect blood for measurement of serum IL-1β levels by ELISA (Invitrogen 88-7013-22). At 4 hours ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was collected and ELISA was performed to detect IL-6 (8 h post stimulation, Cat # 88-7066-22, Invitrogen) and Lcn2 (24 h post stimulation ...
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bioRxiv - Microbiology 2022Quote: ... To measure the protein concentration of IFN-λ1 and -λ2 present in the SN we performed an ELISA following the protocol from the manufacturer: IFN-λ1 (IL-29) from Invitrogen/Thermo Fisher Scientific cat ...
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bioRxiv - Molecular Biology 2022Quote: ... the phage enrichment was evaluated by pooled phage ELISA in 384-well Flat bottom Nunc MaxiSorp plates (Nunc, Roskilde, Denmark). Bait proteins and GST control were immobilized (5 μg in 50 μl PBS per well ...
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bioRxiv - Systems Biology 2024Quote: ... We detected proteins by chemiluminescence using the SuperSignal® ELISA femto maximum sensitivity substrate (Thermo Fisher Scientific™, Waltham, USA) according to the manufacturer’s instructions and the Amersham™ Imager 600 (GE Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2024Quote: ... Triton- and Guanidine-soluble samples were subjected to analysis using species- and isoform-specific antibodies in Soluble and Insoluble Aβ ELISA (Invitrogen, Life Technologies ...
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bioRxiv - Pathology 2024Quote: ... and C-X-C Motif Chemokine Ligand 1 (CXCL1) levels were measured in liver protein lysates and EDTA-plasma by enzyme-linked immunosorbent assay (ELISA) (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Sera competition ELISA assay plates were coated with PBS containing 250 ng of anti-6x His tag monoclonal antibody (Invitrogen) and left covered overnight at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... the amount of IL-10 in the HDL-rich fraction and the (V)LDL-rich fraction was quantified with an IL-10 ELISA (Invitrogen) and used to calculate the fraction of bound IL-10 ...
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bioRxiv - Biochemistry 2024Quote: ... deglycosylated GCase and the GCase N370S mutant were solid-phase coated on the bottom of a 96-well ELISA plates (Maxisorp Nunc-immuno plate ...
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bioRxiv - Cell Biology 2022Quote: ... HMGB1 was measured in cell-free supernantants using an enzyme-linked immunosorbent assay (Tecan HMGB1 ELISA, cat# ST51011, Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... pH 7.2 (PBS) and 100 ul added to wells of a 96-well medisorp ELISA plate (Thermo Fisher Scientific #467320) and incubated overnight at 4° C ...
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bioRxiv - Microbiology 2024Quote: ... hybridomas were screened by enzyme-linked immunosorbent assay (ELISA) using Maxisorp high binding 96 well plates (442404, Thermo Fisher Scientific) coated with 100 ng of FimH-DSG WT protein ...
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bioRxiv - Microbiology 2024Quote: ... The plates were washed 3 times with PBS/Tween 20 0.05% (PBS-T) and saturated for 2 h at room temperature (RT) with 300 µl per well ELISA/ELISPOT diluent 1X (Invitrogen 00-4202-56 ...
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bioRxiv - Microbiology 2024Quote: ... Antigen-antibody reactions were developed by the addition of 1-Step™ TMB ELISA Substrate Solution (Invitrogen™, MA, USA) followed by ELISA Stop Solution (ThermoFisher Scientific ...
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bioRxiv - Systems Biology 2024Quote: ... Soluble VCAM-1 levels were measured by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s protocol (Invitrogen, Waltham, MA).
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bioRxiv - Molecular Biology 2024Quote: ... After another rinse and three more washes the plate was developed with 1-Step™ Ultra TMB-ELISA Solution (ThermoFisher) before stopping the reaction with an equal volume of 2M H2SO4 ...
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bioRxiv - Pathology 2023Quote: ... and then with the appropriate secondary antibody (Alex Fluor 488/555/647 anti-rabbit IgG, anti-mouse IgG1, anti-mouse IgG2a, anti-mouse IgG2b, or anti-goat IgG; 1:1,000, Invitrogen, Grand Island, NY, USA). Nuclei were counterstained with DAPI (#62248 ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was isolated and purified from cultured mouse TPCs (passage 1) with Trizol followed by complementary DNA synthesis with RevertAid™ H Minus kit (#K1632 Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated from spleen and mesenteric lymph nodes single cells suspensions by positive separation using a Dynabeads FlowComp Mouse CD4 Kit (11461D, Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: RNA was prepared from total mouse brains of mice and from BECs using Trizol and Cells-to-CT kit (Thermo Fisher Scientific), respectively ...
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bioRxiv - Microbiology 2022Quote: Lysates extracted from mouse lung tissues with a bead shocker were processed and digested using an EasyPep Mini MS Sample Prep Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... ME) using the EasySep™ mouse T cell isolation kit (StemCell, Cambridge, MA) and labeled with carboxyfluoroscein succinimidyl ester (CFSE; Life Technologies) as described previously [18] ...
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bioRxiv - Immunology 2021Quote: ... Insertion of single base pair changes for the generation of human and mouse FOXN1 variants was achieved by site-directed mutagenesis using the Phusion site-directed mutagenesis kit (Thermo Fisher Scientific).Specific primers for the introduction of single base pair deletions or single base pair changes were designed using the following software www.agilent.com/store/primerDesignProgram.jsp ...
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bioRxiv - Developmental Biology 2020Quote: ... biotinylated with Ovation kits from NuGEN (San Carlos, CA) and hybridized to the Affymetrix mouse genome 430 2.0 array (Affymetrix, Santa Clara, CA). Each array was probed with RNA isolated from an individual mouse ...
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bioRxiv - Immunology 2021Quote: T cells were enriched from the lymph nodes and spleen of mice using the Dynabeads untouched mouse T cell kit (Thermo Fisher Scientific) or the CD4+ T cell selection kit (Miltenyi) ...
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bioRxiv - Neuroscience 2022Quote: ... > 8.0 were further processed with the Affymetrix WT Plus kit and hybridized to Clariom S mouse arrays as described by the manufacturer (Thermo Fisher, Germany). Briefly ...
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bioRxiv - Immunology 2022Quote: ... RNA-seq libraries were prepared with 1 ng of total RNA using an Ion AmpliSeq Transcriptome Mouse Gene Expression kit (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: A minimum of four wells of mouse organoids were pooled and lysed following the protocol of the PureLink RNA Mini Kit (Ambion, Life Technologies, #12183025). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... Negative selection of mesenchymal cells via differential adherence on plastic culture dishes was followed by CD45 depletion using simultaneous incubation with Dynabeads untouched mouse T cell kit (Thermo Fisher #11413D) and Dynabeads mouse DC enrichment kit (#11429D ...
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bioRxiv - Immunology 2024Quote: The sandwich ELISA assay was performed with 4 µg/ml coated F(ab’)2 fragments that were generated from full-size antibodies using the Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (Thermo Fisher, 44980) according to the manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: Serum collected from mice were analyzed for type I IFNa and IFNb using Mouse IFN ProQuantum Immunoassay Kit (Invitrogen #A46736 and #A47435), following manufacture’s protocols.
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bioRxiv - Immunology 2022Quote: Wild-type C57BL/6 splenic-T cells are isolated using Stemcell EasySep™ Mouse T-cell Isolation kit (Stemcell #19851) and stained with Cell Trace Violet (ThermoFisher #C34571). These cells are then stimulated with anti-CD3/CD28 beads (Gibco #11456D ...
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bioRxiv - Microbiology 2022Quote: ... TNF-α and IL-5 cytokines using a mouse cytokine 11-plex antibody bead kit (Th1/Th2 Cytokine 11-Plex Mouse ProcartaPlex™ Panel, Cat No# EPX110-20820-901, Thermo Fisher). Preparation of samples ...
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bioRxiv - Neuroscience 2023Quote: ... The level of IL-1β in microglia culture supernatants was measured by Procartaplex Mouse Cytokine and Chemokine kit (cat no: EPXR260-26088-901, Thermo Fisher Scientific) using the MAGPIX plate reader according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... RNA-seq libraries were prepared with 1 ng of total RNA using the Ion AmpliSeq Transcriptome Mouse Gene Expression kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... qPCR of the SARS-CoV-2 nucleocapsid gene was then performed for each mouse and sample using the Applied Biosystems TaqMan RNA to CT One Step Kit (ThermoFisher Scientific 4392938) to measure viral copy number via transcript number with specifications for each reaction that have been described previously [17,25,27,28].
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bioRxiv - Immunology 2023Quote: ... a Luminex assay using the ProcartaPlex™ Mouse Cytokine & Chemokine Panel 1 36-plex kit (#EPXR360-26092-901, ThermoFisher Scientific, Waltham, MA) was performed according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Mono-biotinylated Fab’ fragments of goat anti-mouse Ig(G+M) were labeled with AF546 using an Alexa Fluor 546 antibody labeling kit (Thermo Fisher Scientific). The molar ratio of AF546 to Fab’ in the AF546-labeled Fab’ was ∼2 determined by a Nanodrop spectrophotometer (Nanodrop Technologies).
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bioRxiv - Cell Biology 2019Quote: ... Anti-rabbit or -mouse Alexa 488 and anti-mouse or -rabbit Alexa 568 (Invitrogen) secondary antibodies were used at a 1:250 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... Immunohistochemistry for mCherry (mouse, 1:1000, Takara; goat anti-mouse-alexa594, 1:500, Invitrogen) was used to delineate viral vector spread ...
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bioRxiv - Cell Biology 2022Quote: ... as well as the indicated secondary antibodies: goat α-mouse 488 mouse (Invitrogen A11029), goat α-mouse 568 (Invitrogen A11004) ...
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bioRxiv - Neuroscience 2021Quote: ... mouse GSCs and mouse NSCs were fixed with 1% formaldehyde (Thermo Fisher scientific, 28906) for 10 min and quenched with 0.125 M glycine for 5 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... and Goat anti-mouse IgG-Alexa 633 1:200 (mouse, A21052, Thermofisher, 1:200).
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bioRxiv - Bioengineering 2023Quote: ... an additional 45-min block was performed in mouse-on-mouse blocking reagent (Invitrogen). Sections were incubated with primary antibodies (Supplementary Table S2 ...
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bioRxiv - Microbiology 2023Quote: ... and corresponding mouse mouse IgG1-PE isotype control (cat# 12-4714-42, ThermoFisher Scientific). Cells were washed twice in FW and analyzed by flow cytometry.
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bioRxiv - Physiology 2023Quote: ... A mouse anti-mouse antibody conjugated with Alexa Fluor 647 was used (Invitrogen A21058). 4′,6′-Diamidino-2-phenylindole staining was used to counterstain cellular nuclei ...
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bioRxiv - Immunology 2024Quote: ... Anti-mouse IL-10 and anti-mouse IL-13 antibodies were purchased from Invitrogen, Thermo Fisher Scientific ...