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Citations for Thermo Fisher :
2051 - 2100 of 6394 citations for GITR Human T45A HEK 293 mFc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: HEK 293T-ACE2 cells (BEI NR2511), HEK 293T (ATCC) and Vero E6 (Green Monkey Kidney cell line, ATCC) cells were grown in DMEM (Thermo Fisher Scientific, Waltham, MA). For HEK 293T and 293T-ACE2 cells ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... An inducible D540K hERG HEK293 cell line (T-REx D540K hERG HEK) was generated using the Flp-In T-REx system (Thermo Fisher Scientific, MA, USA). The D540K hERG mutant was made site-directed mutagenesis from the wild-type (WT ...
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bioRxiv - Immunology 2023Quote: ... Plasmids were co-transfected into HEK-293F cells (ATCC, Manassas, VA) in FreeStyle Media using 293Fectin for transient protein expression (Thermo Fisher Scientific, Waltham, MA). Secreted IgGs were purified from cell supernatants with Protein A resin (Roche ...
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bioRxiv - Immunology 2021Quote: ... 50μg of the plasmid was then transfected into Expi293F cells at a density of 3×106 cells/mL in a 250mL shaking flask using the ExpiFectamine 293 Transfection Kit (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). Ninety-six hours later ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant reporter proteins were expressed as a soluble secreted proteins by transient transfection of suspension culture HEK293F cells (FreeStyle™ 293-F cells, Thermo Fisher Scientific, Waltham MA) for wild type glycosylated structures and in HEK293S (GnTI- ...
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bioRxiv - Synthetic Biology 2021Quote: ... The T cells were isolated using the EasySep human T cell isolation kit (Stemcell) and activated with human T-activator CD3/CD28 Dynabeads (Gibco) at a bead:cell ratio of 1:1 ...
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bioRxiv - Microbiology 2021Quote: ... which is composed of two ACE2 ectodomains linked to the Fc portion of the human IgG (Anand et al., 2020).Alexa Fluor-647-conjugated goat anti-human Abs (Invitrogen) were used as secondary antibodies to detect ACE2-Fc and plasma binding in flow cytometry experiments.
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bioRxiv - Molecular Biology 2022Quote: In vitro sumoylation reactions were performed on protein array slides with more than 9000 human proteins spotted (ProtoArray® Human Protein Microarray v5.0, Invitrogen). The reaction was performed by the technical service of Invitrogen with our purified enzymes ...
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bioRxiv - Immunology 2022Quote: ... For Cytokine Multiplex of Human Inflammation Panel (Invitrogen™ Inflammation 20-Plex Human ProcartaPlex™ Panel) (Catalog # EXP20012185901, eBioscience/Invitrogen) procedures were performed following manufacturer protocol and the plate was processed on BioRad Bio-Plex 200 system with Bio-Plex-HTF attachment (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: RNA was isolated from human NSCLC cells and human clinical NSCLC tumor and normal samples by homogenization in Trizol reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... supernatants were diluted 4-fold and assayed with the Amyloid beta 40 Human ELISA Kit and either the Amyloid beta 42 Human ELISA Kit or the Amyloid beta 42 Human ELISA Kit Ultrasensitive (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Enzyme-linked immunosorbent assays (ELISAs) for human IL-8 were performed using the Human IL-8 ELISA Kit (ThermoFisher SCIENTIFIC) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: HMF and MRC5 fibroblasts were transfected with a pool of 3 siRNAs targeting each of the 710 human kinases (Silencer Human Kinase siRNA Library, #A30079 Thermofisher). Fibroblasts (1250 cells/well ...
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bioRxiv - Immunology 2019Quote: ... human PBMC derived T cells were purified by Human T cell isolation kit from Invitrogen (Invitrogen Dynal AS, Oslo, Norway) according to manufacturer’s instruction ...
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bioRxiv - Bioengineering 2019Quote: ... Samples were rinsed and re-suspended in flow buffer containing 25 μL mL−1 of each of the following monoclonal anti-human antibodies per sample: anti-human CD3-AF700 (56-0037-42, ThermoFisher), - CD40L/CD154-FITC (11-1548-42 ...
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bioRxiv - Immunology 2021Quote: ... and total T cells were purified from PBMC suspensions by negative immunomagnetic selection (purity >90%) using the Human Myeloid DC Enrichment Kit (STEMCELL) and the Untouched total human T cell (Invitrogen) kits ...
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bioRxiv - Cancer Biology 2022Quote: ... primary human breast CAFs were transfected with 75 nm human periostin-targeting stealth siRNA (si-POSTN) (ThermoFisher siRNA ID: HSS116400) or 75 nm non-targeting control siRNA (si-Control ...
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bioRxiv - Cell Biology 2022Quote: ... were prepared from fresh human umbilical veins as previously described (Barbieri et al., 1981) and maintained in Human Endothelial SFM (Gibco™ ...
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bioRxiv - Microbiology 2022Quote: ... Surface PCDH1 was stained using human anti-EC7 mAb-3677 (5 µg/mL) followed by anti-human Alexa FluorTM 555 (ThermoFisher) for 1 h at 4°C each ...
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bioRxiv - Microbiology 2022Quote: ... encoding full-length human MCT1 (uniprot ID: P53985) and human basigin (uniprot ID: P35613-2) were cloned individually into pFastBac vector (Invitrogen) for recombinant expression in Sf9 cells ...
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bioRxiv - Molecular Biology 2023Quote: ... human ETAR (residues 21-406) and human ETBR (residues 27-424) DNA was cloned into a modified pFastBac vector (Invitrogen), which contains an N-terminal FLAG tag (DYKDDDD ...
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bioRxiv - Neuroscience 2023Quote: ... The human FUS or human TARDBP cDNA was subcloned into the pENTR™/D-TOPO® vector (Thermo Fisher Scientific). To generate the Gateway destination vector pUAST-DEST ...
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bioRxiv - Cell Biology 2023Quote: Human islets were transfected with 60 nM siRNA targeting human CEBPG (s2901, Silencer Select Pre-designed siRNA, Ambion, Life Technologies), TMEM176A (s226845 ...
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bioRxiv - Cell Biology 2023Quote: Human islets were transfected with 60 nM siRNA targeting human CEBPG (s2901, Silencer Select Pre-designed siRNA, Ambion, Life Technologies), TMEM176A (s226845 ...
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bioRxiv - Neuroscience 2023Quote: ... and secreted signaling proteins were measured in culture supernatants by the Human Immune Monitoring Center (HIMC) at Stanford University using a Human 48-plex Luminex Procarta Immuno-assay (ThermoFisher).
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bioRxiv - Neuroscience 2023Quote: Human colorectal adenocarcinoma (ATCC, #SK809) and human pancreatic carcinoma (ATCC, #CRL1469) cells were grown in McCoy’s 5A medium (Gibco, #16600082) and Dulbecco’s Modified Eagle’s medium (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... and the Human embryonic kidney (HEK293) cells (Gibco) in Freestyle F17 expression medium (Gibco) ...
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bioRxiv - Cancer Biology 2021Quote: ... rabbit anti-human ER(#RM9101,ThermoFisher Scientific, MA). Histological stained slides were scanned using the Aperio ScanScope XT digital slide scanner and positivity analysis for each target gene staining was carried out using Aperio ImageScope V 10.2.1.2314 software ...
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bioRxiv - Cell Biology 2020Quote: ... 10 ng/ml human basic FGF (Invitrogen, USA), 10 ng/ml recombinant human GDNF (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... 10 ng/ml recombinant human GDNF (Invitrogen, USA) and 1% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: ... mouse anti-human GATA3-PE (clone TWAJ; Invitrogen). BAL cells were collected as described above ...
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bioRxiv - Genomics 2021Quote: ... 20 ng/mL human recombinant EGF (Thermo Fisher), 10 ng/mL cholera toxin ...
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bioRxiv - Microbiology 2021Quote: Human embryonic kidney cells (HEK293FT; Thermo Fisher Scientific), Human hepatocellular carcinoma cells (Huh-7 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... or human IFNγ [1000 U/mL (ThermoFisher, USA)] diluted in DMEM and incubated for 24 hours ...
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bioRxiv - Genomics 2020Quote: ... and Human Brain Total RNA brRNA (ThermoFisher AM7962)) ...
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bioRxiv - Immunology 2022Quote: ... 50 ng/mL recombinant human EGF (Gibco, PHG0314), 80 ng/mL recombinant human R- spondin 1 (R & D systems ...
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bioRxiv - Immunology 2022Quote: ... medium supplemented with 2% human AB serum (Gibco). The concentration of IL-2 was increased to 1000 IU/ml on day 7 and the expanded T cells were electroporated with the indicated mRNA at a concentration of 2 pg mRNA/cell on day 8 using 4D Nucleofector™ System (Lonza ...
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bioRxiv - Developmental Biology 2019Quote: ... BMP2 recombinant human protein (Thermo Fisher Scientific, #PHC7145) was applied for 1h on cells ...
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bioRxiv - Neuroscience 2019Quote: Purified (human) fibrinogen with Alexa 488 (ThermoFisher: F13191) was prepared to a final concentration of 2 mg⋅mL−1 ...
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bioRxiv - Genomics 2019Quote: Human brain total RNA was purchased from ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... anti-rat 568 and anti-Human 647 (Invitrogen) were diluted 1:1000 in PBS-Triton X-100 0.1% with 10% FBS ...
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bioRxiv - Microbiology 2019Quote: Human foreskin fibroblasts were maintained in DMEM (Gibco) supplemented with 3% inactivated fetal serum (IFS ...
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bioRxiv - Bioengineering 2019Quote: ... 10 ng/ml human bFGF (Invitrogen Life Technologies), 20 ng/ml human EGF (Life Technologies) ...
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bioRxiv - Bioengineering 2019Quote: ... 10 ng/ml human bFGF (Invitrogen Life Technologies), 20 ng/ml human EGF (Life Technologies) ...
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bioRxiv - Immunology 2019Quote: Human IgG total Ready-SET-Go (Affymetrix ebioscience) was used to measure total IgG following the manufacturer’s instructions (35) ...
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bioRxiv - Immunology 2021Quote: ... and IFN-γ Human Uncoated ELISA Kit (Invitrogen), respectively ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with 1% human keratinocyte growth supplement (Gibco), 0.2% GibcoAmphotericin B (250 µg/mL of Amphotericin B and 205 µg/mL sodium deoxycholate ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary human aortic smooth muscle cells (Thermo Scientific) and teloHAEC human immortalized aortic endothelial cells (ATCC ...
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bioRxiv - Biochemistry 2021Quote: ... goat anti-human IgG-HRP (Thermo Fisher, SG) was added at 100 μL/well for an incubation of 60 min in dark ...
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bioRxiv - Neuroscience 2021Quote: Human neonatal epidermal keratinocytesl (HEKn) (Life Technologies - C0015C) were cultivated in EpiLife™ Medium (Thermo Fisher Scientific - MEPI500CA ...