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Citations for Thermo Fisher :
2051 - 2100 of 10000+ citations for FSTL1 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: A construct encoding for CDC-20::3xFLAG under a CMV promoter was transfected into Freestyle 293-F cells (Thermo Fisher Scientific). 48 hours later ...
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bioRxiv - Cell Biology 2022Quote: ... over the course of 10 days the FBS-supplemented DMEM was serially diluted with FreeStyle 293 Expression Medium (ThermoFisher Scientific, USA). Once growing in 100% FreeStyle Medium ...
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bioRxiv - Immunology 2022Quote: ... Recombinant antibodies were produced by cotransfecting paired heavy and light chain expression plasmids into Expi293F cells using ExpiFectamine 293 transfection reagents (ThermoFisher Scientific), purified from culture supernatants using the Protein A/Protein G GraviTrap kit (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... Cell surface display DNA constructs for the SARS-CoV-2 spike variants together with a plasmid expressing blue fluorescent protein (BFP) were transiently transfected into Expi293F cells using ExpiFectamine 293 reagent (ThermoFisher Scientific) per manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein transport inhibition in T-REx-293 cell culture was achieved by the application of 1 µl/ml BD GolgiPlug (Fisher Scientific), diluted in appropriate culture medium ...
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bioRxiv - Immunology 2022Quote: ... Cells grown to a density of 3 million cells per mL were transfected using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) and cultivated for four days prior to supernatent harvest ...
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bioRxiv - Molecular Biology 2022Quote: The stable cell line expressing HMGN5 (HMGN5-FlpIn) was created using the T-REx™-293 Flp-In system (Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... 400 μg of calmodulin and myosin-5 expression vectors (at a 1:6 ratio) were mixed with 15 ml FreeStyle 293 media (Thermo Fisher) and 1.2 ml of PEI (Polysciences ...
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bioRxiv - Biophysics 2023Quote: ... inducible Flp-In T-Rex-293 cell line expressing human RyR2 was generated using the Flp-In T-REx Core Kit (Invitrogen, USA) as described before (54) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells grown to a density of 3 million cells per mL were transfected with the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) and cultivated for four days at which point the supernatant was harvested ...
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bioRxiv - Biochemistry 2023Quote: ... The generation of stable expression cell lines in WDR76 knockout background was similar to the stable transfection of Flp-In™-293 Cell Line described by manual (Invitrogen). All cell lines were maintained in DMEM medium with GlutaMAX ...
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bioRxiv - Immunology 2023Quote: ... cells were grown to a density of 3 × 106 cells/mL and transfected using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific). Three to 5 days post-transfection ...
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bioRxiv - Biochemistry 2022Quote: ... Cell surface display DNA constructs for the SARS-CoV-2 G614 or its mutants or S2 together with a plasmid expressing blue fluorescent protein (BFP) were transiently transfected into Expi293F cells using ExpiFectamine 293 reagent (ThermoFisher Scientific) per manufacturer’s instruction ...
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bioRxiv - Immunology 2022Quote: ... All fusion proteins were expressed in Expi-293F cells in 250-280 ml culture using the ExpiFectamine 293 Transfection Kit (Life Technologies) according to the manufacturer’s recommendations ...
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bioRxiv - Genetics 2023Quote: ... and human embryonic kidney 293 cells (HEK293) were purchased from ATCC and cultured in Dulbecco’s Modified Eagle Medium (DMEM) high glucose (Fisher Scientific, #MT10013CV) with 10% FBS at 37 °C and 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: The Flp-In cell line expressing GFP-CENP-E2055-2608 was generated using HeLa T-REx Flp-In 293 cells according to the Flp-In system protocol (Thermo Fisher). GFP-CENP-E2055-2608 was cloned into pcDNA5 FRT/TO vector ...
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bioRxiv - Microbiology 2023Quote: ... All plasmids used in this study were generated by ATUM Bio and transient transfection was carried out following the manufacturer’s protocol for the ExpiFectamineTM 293 transfection kit (Thermo Fisher Scientific). Four days after transfection ...
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bioRxiv - Neuroscience 2023Quote: ... including Camuiα were transfected into HEK293S GnTI-cells (2 x 106 cells per well in 6-well plates) cultured in FreeStyle 293 (Thermo Fisher), using the TransIT2020 transfection reagent (Mirus Bio) ...
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bioRxiv - Molecular Biology 2023Quote: ... The plasmids were co-transfected into Expi293F cells at a 2:1 (HC:LC) mass ratio using ExpiFectamine 293 Reagent (Thermo Fisher Scientific). At 7 days post-transfection ...
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bioRxiv - Immunology 2023Quote: ... The plasmids were transiently co-transfected into Expi293F cells at a mass ratio of 2:1 (HC:LC) using ExpiFectamine 293 Reagent (Thermo Fisher Scientific). After transfection ...
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bioRxiv - Microbiology 2023Quote: HEK293S GnTI-(ATCC CRL-3022) suspension cells used for expression of HIV Env proteins were maintained in FreeStyle 293 expression medium (Life Technologies), supplemented with 1% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2024Quote: ... HIV-1 Env and ELC07 Fab used for cryo-EM were produced by transient transfection of Expi293 cells with endotoxin-free preparations of recombinant plasmids using ExpiFectamine 293 (Fisher Scientific). To produce ELC07 Fab ...
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Phospho-KNL-1 recognition by a TPR domain targets the BUB-1–BUB-3 complex to C. elegans kinetochoresbioRxiv - Cell Biology 2024Quote: ... elegans BUB-1 TPR (1-189)::3xFLAG under a CMV promoter was transfected into Freestyle 293-F cells (Thermo Fisher Scientific). 48 hours later ...
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bioRxiv - Biochemistry 2024Quote: ... Expi293F cells were grown to a density of 3 x 106 cells/mL and transfected using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) and expression carried out for 3 to 5 days post-transfection at 37°C with 8% CO2 ...
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bioRxiv - Bioengineering 2024Quote: ... A mixture of 15 μg of pcDNA3.4-V-gene-mIgG1 vector and 15 μg of pcDNA3.4-V-gene-kappa vector were transfected into Expi293 cells using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... a kind gift from Jason McLellan) was expressed by transiently transfecting transiently transfecting plasmid using FreeStyle 293-F cells (Thermo Fisher) using polyethyleneimine ...
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bioRxiv - Bioengineering 2024Quote: ... cells were diluted to 3 × 106 cells/mL in antibiotic-free Expi293 Expression Medium and transfected using an ExpiFectamine 293 Transfection Kit (Thermo Scientific). ExpiFectamine 293 Transfection Enhancers 1 and 2 were added 20 h post transfection and cultures were incubated for 5 days post-transfection ...
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bioRxiv - Physiology 2024Quote: ... and TaqMan primers (mouse Panx1 mm0045091, mouse Panx2 mm01308054, mouse Pan×3 mm00552586, mouse Gja1 mm00439105, mouse Gja5 mm00433619– Invitrogen) (Rat Panx1 Rn01447976_m1 – Thermo Fisher Scientific ...
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bioRxiv - Genetics 2019Quote: HEK cells were transfected with plasmids encoding GFP-tagged Cdc42 variants using Calcium Phosphate transfection or Lipofectamine LTX Kit (Life Technologies).
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bioRxiv - Cell Biology 2022Quote: ... Transfer and packaging vectors were transfected into HEK 293T cells to produce lentiviruses using Lipofectamine 3000 reagent (ThermoFisher Scientific, MA, USA). Lentiviruses were harvested 48hr post transfection ...
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bioRxiv - Immunology 2022Quote: ... In-house expressed antibodies were made through co-transfection of heavy and light chain plasmids in Expi293 HEK cells (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: The human embryonic kidney (HEK) 293FT cells a high transfection efficiency isolate of the HEK293T cells were purchased from ThermoFisher (# R70007), and used without further authentication ...
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bioRxiv - Molecular Biology 2019Quote: HEK-293T cells (293T/17, ATCC) were maintained in high-glucose DMEM supplemented with 10% fetal bovine serum (FBS, Life Technologies). U2OS cells (ATCC ...
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bioRxiv - Genomics 2021Quote: ... Human embryonic kidney (HEK) 293T cells (CRL-11268, ATCC) were cultured in Dulbecco’s Modified Eagle’s Medium (11965-092, Gibco, Gaithersburg, MD, USA) supplemented with 10% (16000044 ...
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bioRxiv - Cell Biology 2021Quote: ... and pCMV-dR8.91 (Zufferey et al., 1997) (5 mg each) were transfected into HEK 293T/17 cells using Lipofectamine LTX (Thermo Fisher Scientific). Media containing lentivirus was collected 48 h and 72 h following transfection and filtered using a 13 mm syringe filter with a 0.45 µm PVDF membrane (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... envelope and one of the transfer plasmids were co-transfected into human embryonic kidney (HEK 293T) cells using Lipofectamine 3000 (ThermoFisher Scientific) according to the manufacturer’s lentiviral production protocol ...
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bioRxiv - Cancer Biology 2021Quote: A lentiviral pLKO.1-puro constructs targeting KSR1 and non-targeting control were transfected into HEK-293T cells using trans-lentiviral packaging system (ThermoFisher Scientific). The virus was collected ...
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bioRxiv - Physiology 2022Quote: ... ATCC) and human HEK-293T cells (catalog no. CRL-3216, ATCC) were grown in DMEM (catalog no. 31966-021, ThermoFisher Scientific) supplemented with 10% Fetal Bovine Serum (catalog no ...
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bioRxiv - Microbiology 2022Quote: ... The human anti-TNFα IgG1 monoclonal antibody was expressed in HEK-293F FreeStyle cell line expression system (Life Technologies, Carlsbad, CA) with co-transfection of vectors encoding p21 ...
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bioRxiv - Cancer Biology 2022Quote: ... MSCV KSR2 IRES GFP resistant to binding hairpin sh5 (sh5RKSR2) was transfected into HEK-293T cells using trans-lentiviral packing system (ThermoFisher Scientific). The virus was collected 48 hours post transfection and used to infect KP1 sh5 cells with 8 ug/mL Polybrene for 72 hours ...
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bioRxiv - Biophysics 2022Quote: 106 HEK cells were plated in 6-well plates and transfected with the indicated plasmids with Lipofectamine 2000™ reagent (Invitrogen). 24 hours after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Flag-CTPS1H355 and TRE-promoter driven myc-IMPDH2 and myc-IMPDH2Y12A were delivered into HEK 293T or HeLa cells with TurboFect™ transfection reagent (R0532, ThermoFisher). The expression of myc-IMPDH2 was turn off with culture medium containing 1 μg/ml doxycycline (ab141091 ...
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bioRxiv - Microbiology 2020Quote: ... New York University) and a pcDNA3.1 plasmid expressing the SARS-CoV2 glycoprotein into HEK 293T cells using Lipofectamine 3000 (Thermo Fisher Scientific). The supernatant was collected 48 h after transfection ...
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bioRxiv - Molecular Biology 2020Quote: Human embryonic kidney (HEK) 293T cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Thermo Fisher, Waltham, MA, USA; Cat#10566016) supplemented with 10% fetal calf serum (FBS ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells (INS-1 832/13 and HEK-293T) were seeded onto poly-D-lysine coated 8-well chambered cover glasses (Lab-Tek, Thermo Scientific) at a cell density of 70,000 cells/cm2 ...
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bioRxiv - Cell Biology 2021Quote: ... Expression characteristics of each plasmid were evaluated in HEK 293T cells by transient transfection using Lipofectamine™ 3000 (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... Lenti-X Human Embryonic Kidney (HEK) cells were transfected with the sgRNA plasmids using Lipofectamine 3000 (Life Technologies, Cat. No. L3000150), then cultured for 2-3 days in the following media ...
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bioRxiv - Immunology 2022Quote: ... HepG2 and HEK 293T cell lines were cultured in Dulbecco’s modified eagle medium (DMEM high glucose, Life Technologies, Carlsbad, CA, USA), supplemented with 10 % (v/v ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... SNAP-tagged human β2-adrenoceptor or human EP2 receptors fused at the C terminus to a thermostable Nanoluciferase (Hek-ssβ2-AR-tsNluc) using Lipofectamine 3000 reagent (Invitrogen, Paisley, UK). Original SNAP tag and Nanoluciferase sequences were from NEB (Hitchen UK ...
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bioRxiv - Immunology 2023Quote: ... Plasmids were transiently transfected in HEK-293T cells using polyethyleneimine (PEI, PolyAR) in a 1:2.5 DNA:PEI ratio in OptiMem medium (Thermo Fisher Scientific, 22600050). The supernatant containing recombinant proteins was harvested 72 h post-transfection and centrifuged at 10,000 g for 20 min at 4°C ...