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Citations for Thermo Fisher :
2051 - 2100 of 4324 citations for 6 HYDROXY CHRYSENE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: HEK293T cells were seeded in triplicate in 6 well plates at a density of 150,000 cells/well in Dulbecco’s Modified Eagle’s Medium (Gibco) with 10% fetal bovine serum (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... from E17 C57BL/6 mice and primary rat cortical neurons (RCNs; Thermo Fisher Scientific, cat. no. A36512) from E18 Sprague Dawley rats were thawed and cultured according to the manufacturer’s recommendations in a B-27 Plus Neuronal Culture System (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI) staining was used to visualize the nuclei (Thermo Fisher Scientific). Fluorescence was assessed using a fluorescence laser microscope (LSM780 ...
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bioRxiv - Cell Biology 2019Quote: ... 200 μL of PBS containing 1.5 μg/mL DAPI (4’,6-diamidino-2-phenylindole; Thermo Fisher Scientific) was added for 30 min and exchanged to PBS ...
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bioRxiv - Genomics 2019Quote: ... Size analysis was performed for the MinElute purified Exo-treated products on 6% polyacrylamide TBE gels (Invitrogen) (Supplementary Fig ...
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bioRxiv - Biophysics 2019Quote: ... Cells were transfected with 1:1 mEmerald-Zyxin-6 to mCherry-Paxillin-22 using Lipofectamine 3000 (ThermoFisher) per the manufacturer’s protocol and allowed to grow for 24 hours ...
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bioRxiv - Biochemistry 2020Quote: HEK293 cells grown to 30-40% confluence in 6-well dishes were transfected with Lipofectamine 2000 (Invitrogen) in 35mm plates with 0.5 μg of pLKO shRNA or pLVX expression plasmids (Takara) ...
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bioRxiv - Microbiology 2019Quote: Amounts of IFNγ and IL-6 in BALF were estimated by ELISA Ready-SET-Go! Kit (Invitrogen) according to manufacturer’s protocol.
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bioRxiv - Genomics 2019Quote: ... Sections were stained with DAPI (4’,6’- diamidino-2-phenylindole) and mounted in Prolong Gold (Life Technologies). Images were obtained by confocal microscopy (Nikon C2+ Eclipse ...
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bioRxiv - Molecular Biology 2019Quote: ... and adapter-dimers were removed by electrophoresis of the libraries on Novex 6% TBE gels (EC62655BOX, Invitrogen) at 120 V for 1 hour ...
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bioRxiv - Bioengineering 2019Quote: ... cell culture medium was removed from the hydrogels and 300 µl of 6% Alamar Blue® (Thermofisher) solution in serum-free medium were added ...
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bioRxiv - Genomics 2019Quote: ... stained with 4-6-diamidino-2-phenylindole (NucBlue™ Fixed Cell ReadyProbes™ Reagent, R37606, Thermo Fisher) for 5 minutes at room temperature and mounted onto glass slides using ProLong™ Gold antifade reagent (Molecular Probes ...
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bioRxiv - Microbiology 2019Quote: ... cell nuclei were stained with 30 nM DAPI (4′,6-diamidino-2-phenylindole, Invitrogen, Eugene, OR, USA) for 5 min and the coverslips were mounted onto glass slides with CitiFluor AF1 antifading (Electron Microscopy Sciences ...
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bioRxiv - Developmental Biology 2019Quote: ... qPCR reactions were performed on the Applied Biosystems Quantstudio 6 Flex Real-Time PCR System (ThermoFisher Scientific) using 10 ng and 20 ng of cDNA for muscle and liver/adipose tissue respectively ...
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bioRxiv - Molecular Biology 2019Quote: ... The cells were seeded into 6-well plates and transfected using LipofectamineTM 3000 Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... qPCR analysis was carried out in a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific) with Fast SYBR Green Master Mix (Applied Biosystems 4385612) ...
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bioRxiv - Cell Biology 2021Quote: ... Age-matched control male C57BL/6 animals received a transurethral (t.u.) instillation of PBS (Gibco, Paisley, UK) and were kept in separate cages.
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bioRxiv - Biochemistry 2019Quote: ... Knockdown of PALB2 (Buisson et al., 2017a) was performed 6-8 h later using Lipofectamine RNAiMAX (Invitrogen). Twenty-four hours post-transfection ...
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bioRxiv - Biochemistry 2020Quote: ... PCR products were then mixed with Trackit cyan/orange Loading buffer 6 X (ThermoFisher Scientific, Waltham, Massachusetts), loaded on a 2.5 % agarose gel and migrated for 30 min at 100 V using the Mupid-One electrophoresis system (Dominique Dutscher ...
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bioRxiv - Physiology 2021Quote: ... Quantitative real-time PCR was performed in ViiA7 and QuantStudio 6 real-time PCR systems (Applied Biosystems) using the SYBR Green PCR Master Mix (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2021Quote: ... 4 × 105 cells were seeded in 6-well plates and cultured with RPMI 1640 medium (ThermoFisher # 11875093) containing 10% FBS (Seradigm) ...
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bioRxiv - Microbiology 2021Quote: ... Real-time PCR was performed using a QuantStudio 6 Flex Real-Time PCR detection system (Thermo Fisher) and PerfeCTa SYBR Green FastMix (Quantabio ...
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bioRxiv - Immunology 2021Quote: ... Sorted memory CD4+ T cells were labelled with 5-(and 6)-carboxyfluorescein diacetate succinimidyl ester (CFSE, ThermoFisher) and cultured at a ratio of 2:1 with irradiated autologous monocytes untreated or pulsed for 3 h with recombinant SARS-CoV-2 Spike protein (2.5 μg/ml) ...
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bioRxiv - Physiology 2019Quote: ... Then cells were seeded on Collagenase I coated petri dishes (Collagen I, Coated Plate 6 Well, Gibco Life technologies A11428-01 ...
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bioRxiv - Immunology 2021Quote: ... Reactions were carried out on a QuantStudio 6 and 7 Flex Real-Time PCR System (Applied Biosystems) according to the manufacturer’s specifications ...
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bioRxiv - Immunology 2021Quote: ... Reactions were carried out on a QuantStudio 6 and 7 Flex Real-Time PCR System (Applied Biosystems) according to the manufacturer’s specifications ...
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bioRxiv - Neuroscience 2020Quote: ... Comparative Ct measurements including the melting curve were quantified using QuantStudio 6 flex qPCR device (Life technologies). The geometric mean of two housekeeping genes (ef1a and odc ...
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bioRxiv - Biochemistry 2021Quote: ... 6 µl of the sample was analysed on SDS-PAGE (NuPAGE Bis-Tris Gel, Thermo Fisher Scientific) by heating at 95 °C for 5 min with 2 µL of SDS-PAGE loading dye (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lines were passaged every 4-6 days and MIN6 were detached with 0.25% trypsin-EDTA (Gibco) and seeded at 7.5-1×106 cells per 75 cm2 flask ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Alexa Fluor™ 488 (#C10337) and 4’,6-diamidino-2-phenylindole (DAPI; #62248) were purchased from Invitrogen-ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... Lipofection was performed in a 6-well dish using CRISPRMAX™ Cas9 Transfection Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative real-time PCR was performed on a real-time PCR system (QuantStudio 6 Flex, Applied Biosystems) with SYBR Green PCR Master Mix (Life Technologies) ...
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bioRxiv - Microbiology 2019Quote: ... the cells were incubated with Dapi (4′,6-Diamidine-2′-phenylindole dihydrochloride) in mounting medium (Molecular Probes). Afterward ...
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bioRxiv - Microbiology 2019Quote: ... the cells were incubated with Dapi (4′,6-Diamidine-2′-phenylindole dihydrochloride) in mounting medium (Molecular Probes). Afterward ...
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bioRxiv - Molecular Biology 2021Quote: ... mESCs were seeded to 6-well plates and each well was transfected with Lipofectamine 3000 (ThermoFisher #L3000015) or nucleofection (Lonza #VPH-1001 ...
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bioRxiv - Immunology 2020Quote: ... plates were incubated with 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) substrate (ABTS, Thermo Fisher Scientific) for 15 min at RT shielded from light and absorbance was measured at optical density (OD ...
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bioRxiv - Immunology 2021Quote: ... 5.5×105 293T were transfected with 2.5µg pDNA in 6-well plates using Lipofectamine 3000 (Invitrogen #L3000015) transfection reagent following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... The nucleus was stained with 50 ng/ml 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen Cat. #D1306) for 10 min at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... Prior to differentiation they were seeded into 6 well or 12 well plates treated with Geltrex (Gibco) diluted 1:50 with DMEM/F-12 ...
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bioRxiv - Developmental Biology 2020Quote: ... coated 6-well tissue culture plates (Costar) in StemPro medium comprising of DMEM/F12 with Glutamax (Invitrogen), 1.8% BSA (Invitrogen) ...
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bioRxiv - Physiology 2020Quote: ... 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI) staining was used to visualize the nuclei (Thermo Fisher Scientific). Fluorescence was assessed using a fluorescence laser microscope (LSM780 ...
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bioRxiv - Genetics 2019Quote: ... 6 µl of the Lipofectamine 3000 transfection reagent was added to 125 µl of Opti-MEM (Gibco) to which 2.5 µg of template plasmid had been added (cotransfection of ZP1MT or ZP1WT with ZP2 ...
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Glycogen Synthase Kinase 3 regulates the genesis of the rare displaced ganglion cell retinal subtypebioRxiv - Neuroscience 2021Quote: ... Sections were counterstained with 1:1000 4′,6-diamidino-2-phenylindole (DAPI) (1 mg/mL, Thermo Scientific).
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bioRxiv - Genomics 2021Quote: ... The protocol included an additional size selection step after library preparation on a 6% TBE gel (Invitrogen). 50bp single-end sequencing of our samples was performed on an Illumina HiSeq 4000 at the Vincent J ...
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bioRxiv - Cell Biology 2019Quote: ... A 6-well plate was transfected with 4 µg of the appropriate plasmid using Lipofectamine 2000 (ThermoFisher) according to the manufacturer’s instructions ...
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An Azobenzene G-quadruplex Ligand Exhibits Promising Antibacterial Activity against Escherichia colibioRxiv - Microbiology 2022Quote: ... labelled with Tandem Mass Tag (TMT) 6-plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific) and the labelled samples pooled.
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bioRxiv - Neuroscience 2022Quote: ... pre-coated 6-well plates at a density of 1.25×106 cells/mL using Neurobasal A (Invitrogen) supplemented with 1x B27 (Invitrogen) ...
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bioRxiv - Biochemistry 2022Quote: ... Aliquots of the eluate were either supplemented with 6 x gel loading dye containing SDS (Thermo Scientific), heated to 65° for 10 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... before pooling desired fractions and concentrating with Pierce PES 2-6 mL concentrator columns (Thermo Fisher Scientific). Concentrated protein was aliquoted and flash frozen in liquid N2 before storage at -80°C.
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bioRxiv - Cell Biology 2022Quote: ... Transfection with 5 ug total DNA in a 6 well plate was performed using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocol ...