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Citations for Thermo Fisher :
2051 - 2100 of 10000+ citations for 6 Chloropyrazolo 1 5 a pyridine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 2% B-27 and 1% of N-2 supplements (GIBCO), 1% L-glutamine ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2 μg mL−1 puromycin (58-58-2, ≥98%, Invitrogen) and 100 μg mL−1 hygromycin (31282-04-9 ...
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bioRxiv - Neuroscience 2024Quote: ... 2% B-27 and 1% of N-2 supplements (GIBCO), 1% L-glutamine ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml phenol:chloroform 5:1 pH 4.5 (Thermofisher #AM9720) and 0.5 ml glass beads (Sigma #G8772) ...
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bioRxiv - Cancer Biology 2021Quote: ... 1% L-glutamine and 1% MEM Non-essential amino acids (MEM NEAA) (Gibco, 11140-050). For SaOS-2 cells ...
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bioRxiv - Cell Biology 2021Quote: ... 1% SDS) and pre-heated acid phenol:chloroform:IAA (125:124:1, ThermoFisher Scientific, Catalog Number: AM9722), and were incubated at 65°C for 30 min with shaking ...
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bioRxiv - Cancer Biology 2022Quote: ... 1% v/v non-essential amino acids (NEAA) and 1% v/v penicillin-streptomycin (Invitrogen). Corresponding cell line origins ...
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bioRxiv - Molecular Biology 2021Quote: ... was diluted to 1 mg/ml using 10 mм hydrochloric acid (SA56-1, Fisher Scientific), and then mixed 1:1 with 0.2 M sodium carbonate–bicarbonate buffer (24095 ...
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bioRxiv - Biophysics 2023Quote: ... 1× PS and 1× MEM non-essential amino acids (NEAAs; catalog no. 11140-050, Gibco). Cells were cultured in a humidified atmosphere with 5% CO2 at 37 °C and passaged every 2 or 3 d ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% MEM non-essential amino acids solution and 1% MEM vitamin solution (all Thermo Fisher). PDAC cell line MIA PaCa-2 was cultured in DMEM supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Molecular Biology 2022Quote: ... medium (1:1) supplemented with N-2 (Gibco), B-27 (Gibco) ...
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bioRxiv - Genomics 2020Quote: ... the upper aqueous phase was recovered and nucleic acids were precipitated by adding 2 μL GlycoBlue (Ambion) and 500 μL isopropanol (incubated at −20°C for 10 min) ...
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bioRxiv - Genetics 2021Quote: ... After an additional 2 PBS washes cells were counterstained with DAPI nucleic acid stain (Thermo Fisher Scientific), diluted 1:1000 with PBS ...
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bioRxiv - Synthetic Biology 2020Quote: ... we used 20 amino acids that contained 2 mM of 13C6 15N2 L-lysine (Thermo Fisher Scientific) and 13C6 15N4 L-arginine (Thermo Fisher Scientific ...
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bioRxiv - Zoology 2023Quote: ... and 2-(N-morpholino)ethanesulfonic acid (MES) running buffer solution (Bolt™, Thermo Fisher Scientific, Waltham, Massachusetts). A standard ladder (Mark12™ ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were maintained in RF10: RPMI 1640 supplemented with 2 mM MEM nonessential amino acid solution (Gibco), 100 mM HEPES (Gibco) ...
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bioRxiv - Microbiology 2023Quote: GAG was subjected to acid hydrolysis with 2 M HCl (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), 100 °C for 3 h to obtain soluble oligosaccharides of various length (max ...
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bioRxiv - Biochemistry 2022Quote: ... Dried digests were reconstituted in 2% acetonitrile/0.1% formic acid and 0.3μg injected onto a LC pre-column (ThermoFisher Scientific Acclaim Pep map 100 ...
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bioRxiv - Biophysics 2021Quote: 106 T-cells were incubated in T-cell media supplemented with 5 µg ml-1 Fura-2 AM (11524766, ThermoFisher Scientific) for 20 min at 24°C ...
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bioRxiv - Neuroscience 2021Quote: ... into jaw closer muscles of 5-8 day old mouse pups with 1-2% dilution of Alexa Fluor 488 hydrazide (Life Technologies), under isoflurane anesthesia.
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bioRxiv - Cell Biology 2020Quote: ... and then incubated overnight at 4°C in 2 ml TBST/5% milk with a 1:1,000 dilution of anti-CPY Monoclonal antibody 10A5B5 (Invitrogen, A-6428). The nitrocellulose was then washed 3 times in 20 ml TBST at room temperature for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... Nuclei were lysed by adding 1:4 SDS buffer (2% SDS, EDTA) and samples were sonicated (5×1s pulses, 80% power, Fisher Scientific). Samples were diluted to 0.2% SDS with TX-100 lysis buffer and centrifuged for 20 min at 20,000 x g ...
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bioRxiv - Developmental Biology 2021Quote: ... Twenty million cells were diluted in 2 mL of GBSS containing 1% FBS and stained with 5 μg/mL of Hoechst 33342 (Invitrogen H3570) for 1 h at 32 °C ...
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bioRxiv - Systems Biology 2020Quote: ... of the immunodepleted pooled CSF samples were separately dissolved in 100 mM TEAB buffer (625 μL) and labeled with 5 mg of TMT 126 channel reagent (cohort 1 lot# TF266326, cohort 2 lot# SG253447, ThermoFisher Scientific) in anhydrous ACN (256 μL) ...
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bioRxiv - Immunology 2021Quote: ... Triton X-100 with 5 % normal-goat-serum and unconjugated F(ab′)2 goat anti-mouse IgG (H+L) antibody (1:500, Thermo Scientific), at 4 °C for 2 h ...
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bioRxiv - Biophysics 2020Quote: 106 T-cells were incubated in T-cell media supplemented with 5 μg ml−1 Fura-2 AM (11524766, ThermoFisher Scientific) for 20 min at 24°C ...
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bioRxiv - Neuroscience 2021Quote: ... we added a 1 mL of the following mixture to the culture media and incubated the cells at 25°C incubator for 1—3 hours: 5 μM Fura-2 AM (F-1201, Life Technologies), 250 μM probenecid (162-26112 ...
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bioRxiv - Cell Biology 2021Quote: ... plasmid DNA at a concentration of 1-2 mg/ml was mixed with 5 µl of Salmon Sperm DNA (10mg/ml) (Thermofisher Scientific) prior to electroporation ...
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bioRxiv - Cell Biology 2021Quote: ... incubation with secondary antibody was performed in 0.5% BSA/PBS for 1 hour at room temperature and nuclei were stained with 2 μg/ml Hoechst 33342 (Life Technologies). Images were acquired using the Leica SP8 confocal or Leica DMI6000-SD microscopes ...
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bioRxiv - Cell Biology 2022Quote: ... Nuclei were lysed by adding 1:4 SDS buffer (2% SDS, EDTA) and samples were sonicated (5×1s pulses, 80% power, Fisher Scientific). Samples were diluted to 0.2% SDS with TX-100 lysis buffer and centrifuged for 20 min at 20,000 x g ...
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bioRxiv - Developmental Biology 2022Quote: ... and dissociated into single cells in 100 μl of Enzyme 1 and 5 μl of Enzyme 2 (Pierce Cardiomyocytes Dissociation Kit, ThermoFisher Scientific) for 30 min at 350 rpm shaking at 30°C ...
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bioRxiv - Cell Biology 2021Quote: Cells were set at a concentration of 1 x105 cells/mL 24 hrs prior to EdU addition (5-ethynl-2’-deoxyuridine; Life Technology, Thermo Scientific) in media free from thymidine prepared using Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were then washed in 0.1 M TB (2 ×5 minutes) and counterstained with Hoechst 33342 (1:20000, #62249, Thermo Fisher Scientific) diluted in 0.1 M TB ...
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bioRxiv - Synthetic Biology 2022Quote: ... The resulting cDNA was diluted 1:10 and 2 μl of each sample was amplified using a QuantStudio 5 (Applied Biosystems) thermal cycler ...
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bioRxiv - Immunology 2019Quote: ... mid-L3 larvae were fed on fly food containing 1 mM 5-ethynyl-2 deoxyuridine (Click-iT EdU, Thermo Fisher Scientific) for 4 hours for hemocyte staining and 3 hours for lymph gland staining at 25°C ...
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bioRxiv - Microbiology 2020Quote: ... ticks were homogenised in a 2 ml reaction tube with two 5 mm steel beads and 1 ml TRIzol Reagent (Thermo Scientific) using a Tissue Lyser II (Qiagen ...
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bioRxiv - Biochemistry 2020Quote: ... cells were incubated with an alpha tubulin monoclonal antibody conjugated to Alexa Fluor 488 (B-5-1-2, Invitrogen, Carlsbad, CA) at 2 μg/mL in 1% BSA in PBS for 1 hour ...
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bioRxiv - Synthetic Biology 2022Quote: ... a 1:10 dilution was performed and 2 μl of diluted DNA was amplified by means of a QuantStudio 5 (Applied Biosystems) thermal cycler using the SYgreen Blue Mix (PCRbio ...
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bioRxiv - Microbiology 2022Quote: ... the robot adds 2 volumes of 30% PEG 6000 (in 1.6 M NaCl) and 35ul of 1:5 diluted Glycoblue (Invitrogen, Thermo Fisher) to each well ...
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bioRxiv - Neuroscience 2022Quote: ... 2 × 5 min) and incubated with secondary antibody (AlexaFluor 488-tagged goat anti-rabbit Ab, 1:1000, ThermoFisher Scientific, A-11008) and with Alexa Fluor 633-conjugated Streptavidin (1:400 ...
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bioRxiv - Biochemistry 2023Quote: ... NaHCO3 and 150 μL acetone containing 4 mg/mL 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide (L-FDAA, Thermo Scientific) was added ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed in PBS (2 x 5 min) and incubated in 0.1% Tween in PBS containing streptavidin Alexa 594 conjugate (ThermoFisher, 1:1500) for 2 hours at room temperature ...
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bioRxiv - Pathology 2024Quote: ... 2 µl drops of yeast suspension were plated in five 1:5 serial dilutions on 100 mm square plates (Thermo Fisher) with selective SD medium and increasing concentrations (0.07–7 µg/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... Digestions were incubated for 1 hour at 37 ℃ and then diluted 1:5 with UltraPure water before loading on E-gel EX 2% Agarose gels (Invitrogen, G401002). Backbones were purified using the Monarch Gel Extraction Kit (Monarch) ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 μl 20× 6-carboxyfluorescein (FAM)-labeled Assay Mix (Applied Biosystems), and 9 μl of cDNA ...
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Safety and efficacy of C9ORF72-repeat RNA nuclear export inhibition in amyotrophic lateral sclerosisbioRxiv - Systems Biology 2021Quote: ... The cells were then split 1:6 with Accutase (Gibco™) onto matrigel substrate in the presence of 10 µM of rock inhibitor (Y-27632 dihydrochloride ...
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bioRxiv - Biophysics 2021Quote: ... and acetyl-α-tubulin primary antibody (clone 6-11B-1, Invitrogen) for acetylated tubulin ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM Phenylmethylsulfonyl fluoride (PMSF; ACROS Organics, Cas#329-98-6), 5 mM β-ME ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μl 20× 6-carboxyfluorescein (FAM)-labeled Assay Mix (Applied Biosystems), and 9 μl of cDNA ...
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bioRxiv - Microbiology 2023Quote: ... anti-Integrin alfa-6 (ITGA6) (1:1000) (MA5-16884, ThermoFisher Scientific) for 1 h at room temperature ...