Labshake search
Citations for Thermo Fisher :
2001 - 2050 of 10000+ citations for rno mir 16 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... on QuantStudio 6 Flex Real-Time PCR System (Life Technologies). 18S rRNA (ribosomal RNA ...
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Candida albicans enhance Staphylococcus aureus virulence by progressive generation of new phenotypesbioRxiv - Microbiology 2024Quote: ... in a StepOnePlus™ Real-Time PCR System (Applied Biosystems) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... QuantStudio 7 Pro Real-Time PCR system (Thermo Fisher Scientific) was used to conduct the real-time PCR assays ...
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bioRxiv - Immunology 2024Quote: ... fluorescence detected on a StepOne real time PCR system (Thermofisher). Reaction efficiency was assessed using a serially diluted standard curve and product by melting curve ...
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bioRxiv - Molecular Biology 2024Quote: ... on a StepOne Plus Real-Time PCR system (Applied Biosystems), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... on a QuantStudioTM 3 Real-Time PCR Instrument (Applied Biosystems). The following primers were used for qRT-PCR: Vcam1 forward primer 5’-AGTTGGGGATTCGGTTGTTCT-3’;Vcam1 reverse primer 5’-CCCCTCATTCCTTACCACCC-3’;C1qa forward primer 5’-AAAGGCAATCCAGGCAATATCA-3’;C1qa reverse primer 5’-TGGTTCTGGTATGGACTCTCC-3’;H2-eb1 forward primer 5’-GCGGAGAGTTGAGCCTACG-3’;H2-eb1 reverse primer 5’-CCAGGAGGTTGTGGTGTTCC-3’;Ccl8 forward primer 5’-TCTACGCAGTGCTTCTTTGCC-3’;Ccl8 reverse primer 5’-AAGGGGGATCTTCAGCTTTAGTA-3’;Il27 forward primer 5’-CTGTTGCTGCTACCCTTGCTT-3’;Il27 reverse primer 5’-CACTCCTGGCAATCGAGA-3’;Gapdh forward primer 5’-AGGTCGGTGTGAACGGATTTG-3’;Gapdh reverse primer 5’-TGTAGACCATGTAGTTGAGGTCA-3’.All data were normalized to Gapdh quantified in parallel amplification reactions.
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bioRxiv - Developmental Biology 2024Quote: ... using the QuantStudio 5 Real-Time PCR system (Applied Biosystems) with the following thermal cycling conditions ...
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bioRxiv - Developmental Biology 2024Quote: ... on a QuantStudio 6 Flex Real-Time PCR unit (ThermoFisher). Changes in RUNX1 expression were calculated using the 2-ΔΔCT methodology.
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bioRxiv - Neuroscience 2024Quote: ... on a QuantStudio7 Flex Real-Time PCR System (Thermo Fisher). Oligonucleotide primer sequences for target and reference genes are as follows ...
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bioRxiv - Plant Biology 2024Quote: ... performed on a StepOnePlus Real-Time PCR System (Applied Biosystems), had a pre-incubation of 50° C for 2 min ...
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bioRxiv - Microbiology 2024Quote: ... on a StepOne Real-time PCR (Thermo Fisher Scientific, USA). The relative mRNA expression level was determined using the 2-ΔΔCt method using chitin synthase I (csh1 ...
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bioRxiv - Immunology 2024Quote: ... using the Step-One Real-time PCR system (Life Technologies). Target mRNA expression was normalized to the expression of GAPDH or β-actin ...
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bioRxiv - Neuroscience 2024Quote: ... in a QuantStudio 5 real-time PCR system (Applied Biosystems) using the fast run mode settings ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative RT-PCR was performed on an ABI7900HT detection system (Life Technologies) using Maxima SYBR Green/ROX qPCR Master Mix (2X ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was diluted 1:10 for quantitative real-time PCR (qPCR) run on an Applied Biosystems QuantStudio™ 6 fast real-time PCR system (Applied Biosystems, Foster City, CA) using PowerUp SYBR green master mix (Life Technologies) ...
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bioRxiv - Genetics 2020Quote: ... The reverse transcription products (cDNA) were amplified by quantitative real-time PCR using a real-time PCR system (7900; Applied Biosystems, Foster City, CA, USA). The relative expression of the target genes was determined using the 2-ΔCt method ...
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bioRxiv - Genetics 2019Quote: ... One μL of diluted cDNA (1:25) was used for real-time PCR in a QuantStuioTM 6 Flex Real-Time PCR System (Applied Biosystems, Foster City, California, USA). Peptidylprolyl isomerase A (PPIA ...
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Rac1, Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneuronsbioRxiv - Neuroscience 2022Quote: ... Real time PCR analysis was performed using a Step One Plus real-time PCR system (Applied Biosystems, Life Technologies, Thermo Fisher Scientific Inc., Waltham, MA). Expression levels of genes encoding Ank-1 (forward primer (F) ...
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bioRxiv - Biochemistry 2024Quote: ... Real-Time Quantitative PCR (qPCR) was performed with the standard protocols in ABI 7500 Fast Real-Time PCR System (Applied Biosystems™ 7500, ThermoFisher, USA) using SYBR Green Master Mix ...
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bioRxiv - Microbiology 2023Quote: The extracted DNA was subjected to 47 kDa real-time PCR on an Applied Biosystems™ 7500 Real-Time PCR System (Thermo Fisher Scientific, MA, USA). This real-time PCR amplifies a 118bp section of 47 kDa gene (htrA ...
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bioRxiv - Cell Biology 2023Quote: ... 10µM forward and reverse primers were used for real time PCR in a StepOnePlus® Real-Time PCR system (all from Applied Biosystems, Waltham, MA, USA). The RT-qPCR amplification protocol comprised a polymerase activation step for 15 minutes at 95 °C ...
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bioRxiv - Neuroscience 2019Quote: ... Quantitative real time PCR (qRT-PCR) reactions were performed in the StepOnePlus or Quant Studio6 Flex Real Time PCR System (ABI, Thermo Fisher) with ten-fold dilution of cDNA and 200 nM of each primer using the SYBR Select PCR Master Mix (ABI ...
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bioRxiv - Biochemistry 2021Quote: ... quantitative real-time PCR was performed in 5 μl of SYBR Green PCR master mix using a real-time PCR system (Applied Biosystems). Amplification products were then analyzed by a melting curve ...
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bioRxiv - Biochemistry 2024Quote: ... Quantitative real-time PCR (qRT-PCR) was performed in a 96-well format using the StepOne Plus Real-Time PCR system (Applied Biosystems) and SYBR Select Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative real-time polymerase chain reaction (qPCR) was performed on a QuantStudio™ 5 Real-Time PCR System (Applied Biosystems) with the Power SYBR Green PCR Master Mix (Applied Biosystems) ...
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bioRxiv - Plant Biology 2020Quote: ... kit and an ABI 7900HT fast real-time PCR system (ThermoFisher Scientific Inc., Waltham, MA, USA). Forty cycles (15 sec ...
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bioRxiv - Plant Biology 2022Quote: ... using kit-specified reaction parameters in a Quant Studio 5 Real-Time PCR machine (ThermoFisher Scientific). Averaged actin and dnaJ values were used as the stably expressed normalization genes ...
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bioRxiv - Immunology 2020Quote: ... and reverse transcription with Maxima First strand cDNA synthesis Kit for real-time PCR (Thermo Fisher) to prepare cDNA ...
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bioRxiv - Microbiology 2021Quote: ... Quantification was performed using a library quantification kit and StepOne Real-Time PCR System (Life Technologies). High-throughput sequencing was performed as paired-end 100 sequencing using the HiSeq X10 system (Illumina).
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bioRxiv - Molecular Biology 2020Quote: ... and subjected to quantitative real-time PCR with the TaqMan gene expression assay kit (Applied Biosystems) on StepOnePlus Real-Time PCR System (Thermo Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Real-time PCR analysis was done by DyNAmo Flash SYBR Green qPCR Kit (Thermo Fisher scientific) using CFX96™ Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Immunology 2020Quote: ... Real-time PCR reactions were set up with a SYBR green power up kit (Applied Biosystems) on an Applied Biosystems 7500 Real Time Quantitative PCR System workstation ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA quality and quantity were measured using NanoDrop™1000 Quantitative RT-PCR was performed using the ViiA7 Real-Time PCR system (Applied Biosystems, Foster City, CA, USA). Each reaction contained 1X final concentration of SYBR Green PCR Master Mix 2X (Applied Biosystems ...
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bioRxiv - Bioengineering 2021Quote: ... QPCR COVID-19 detection was perfomed using the TaqPath COVID-19 CE-IVD RT-PCR kit (Applied Biosystems) on the QuantStudio 5 real-time PCR (Applied Biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... Real-time PCR was performed using Quant Studio Design and Analysis Real-Time PCR Detection System in conjunction with the Maxima SYBR Green qPCR Master Mix (Cat # K0221, Thermo Fisher Scientific) using specific primers for Stmn2 ...
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bioRxiv - Cell Biology 2019Quote: ... Real-time PCR was performed using Quant Studio Design and Analysis Real-Time PCR Detection System in conjunction with the Maxima SYBR Green qPCR Master Mix (Cat # K0221, Thermo Fisher Scientific) using specific primers for Stmn2 ...
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bioRxiv - Neuroscience 2024Quote: ... Cycling and detection were carried out using an Applied Biosystems 7500 Real Time PCR System and data quantified using Sequence Detection Software Version 1.4 (Applied Biosystems, Carlsbad, CA). PCR was performed for a total of 40 cycles (95° for 15 sec ...
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bioRxiv - Cell Biology 2020Quote: ... Transcripts of interest were analyzed using the QuantStudio 5 real-time quantitative PCR (RT-qPCR) system with the associated software (Thermo Fisher Scientific). cDNA was diluted with the Fast SYBR green master mix (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... then subjected to Real-Time quantitative PCR (RT-qPCR) in duplicate using the Step One Plus system (Applied Biosystems, Foster City, CA) with gene-specific primer sets (Supplementary Table 1 ...
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bioRxiv - Plant Biology 2021Quote: Total RNA for real-time RT-PCR was isolated from maize leaves or stems using Trizol® reagent (Invitrogen, Carlsbad, CA, USA) and real-time qPCR was performed using an Mx3000P Multiplex qPCR system (Stratagene ...
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bioRxiv - Molecular Biology 2022Quote: ... DDX39B and FOXP3 RNA levels were measured by real-time quantitative PCR (RT-qPCR) using PowerUP SYBR Green Master Mix (Thermo Fisher Scientific) in a StepOnePlus Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... PowerTrack™ SYBR Green Master Mix and primers for the selected genes (Table S5) were added and RT-qPCR was performed on a StepOnePlus™ Real-Time PCR System (Applied Biosystems). As reference genes we used the Mobk13 (mob4 ...
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bioRxiv - Microbiology 2021Quote: ... following the manufacturer’s protocol and RT-qPCR performed in 384-well plates using a QuantStudio™ 5 Real-Time PCR System (Applied Biosystems™). Primer/probe pairs were ...
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bioRxiv - Neuroscience 2024Quote: ... and TBR1 Hs00232429_m1) were used and RT-qPCR was run in triplicate samples on a QuantStudio™ 1 Real-Time PCR System (ThermoFisher Scientific) with GAPDH as the housekeeping gene (HS02786624_g1)
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bioRxiv - Developmental Biology 2024Quote: ... and analyzed by RT-qPCR using Quant Studio 12K Flex Real Time PCR system version 1.1.2 (Applied Biosystems by Life Technologies, Thermo Fisher Scientific) and validated (efficiency > 1.8 ...
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bioRxiv - Developmental Biology 2023Quote: ... Real-time quantitative PCR (RT-qPCR) was performed in three technical replicates per sample using SYBR Green master mix (Life Technologies, 11733046) on a ViiA 7 Real-Time PCR system (AB Applied Biosystems ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Quantitative RT-qPCR was performed using PowerUp™ SYBR™ Green Master Mix and QuantStudio 3 Real-Time PCR System (ThermoFisher Scientific).
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bioRxiv - Microbiology 2024Quote: ... levels quantitative real-time PCR (RT-qPCR) was performed as previously described22 using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher, #4444436) and an OneStepPlus Real-Time PCR System (96-well format ...
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bioRxiv - Genomics 2020Quote: ... Quantitative real-time PCR (qRT-PCR) was carried out on ABI7900 with PowerUp SYBR Green Master Mix kit (Applied Biosystems), according to the recommendations of the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... one-step quantitative real-time PCR was performed using SuperScript III Platinum SYBR Green One-Step qRT-PCR Kit (Invitrogen) with primers specific for the TRS-L and TRS-B sites for the N gene as well as ACTB as an internal reference ...