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Citations for Thermo Fisher :
2001 - 2050 of 10000+ citations for Zika Virus NS1 Protein Uganda strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... 3 wells from each strain were fixed with 10 µl of 16% paraformaldehyde (Fisher Scientific Cat. No. 50-980-487) and stored at 4°C ...
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bioRxiv - Genomics 2022Quote: ... Using the DNA we again confirmed strain identity by sequencing the ITS region and nucleic acid quantity was measured using Qubit (ThermoFisher) according to the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2022Quote: ... 5’ and 3’ RACE ready cDNA was generated from RNA extracted from ~30 pairs of ovaries or testes dissected from 5-7 days-post-emergence (dpe) Liverpool strain adults using Trizol (Invitrogen). Primers LA1076 then nested with LA1352 (5’ ...
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bioRxiv - Microbiology 2024Quote: ... One fluorescent variant of aEPEC 1711-4 was obtained by transforming the wild-type strain with the recombinant plasmid pDP151 (Invitrogen), which encodes the fluorescent protein mCherry and confers resistance to ampicillin.
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bioRxiv - Biochemistry 2024Quote: ... Ligations of the restricted ORF fragments into the pYES2 vector were transformed into competent Escherichia coli TOP10™ strain (Invitrogen) and grown in Luria-Bertani (LB ...
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bioRxiv - Synthetic Biology 2023Quote: ... All yeast strains are routinely stuck out on a sterile YPAD plate consisting of 20 g/L dextrose (Fisher Scientific), 20 g/L peptone (Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... derived from the MHOM/NP/02/BPK282/0 strain was maintained as promastigotes at 26°C in HOMEM medium (Gibco, ThermoFisher) supplemented with 20% Foetal Bovine Serum ...
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bioRxiv - Neuroscience 2023Quote: Mouse embryonic cerebral cortices (strain C57BL/6N) were harvested at gestational day 14.5 (E14.5) and digested with Trypsin (Gibco, 15090-046). To obtain a single cell suspension the cells were mechanically dissociated with a glass Pasteur in neuronal culture medium (Neurobasal™ Plus Medium ...
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bioRxiv - Plant Biology 2023Quote: ... All direct assays and library screens were performed using the MaV203 yeast strain as described in the ProQuest manual (Invitrogen). Before screening the cDNA libraries ...
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bioRxiv - Molecular Biology 2023Quote: ... xanthus WT strain DK1622 and gene expression was quantified using the relative standard curve method (user bulletin 2; Applied Biosystems). 16S rRNA was used as the internal standard for each sample ...
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bioRxiv - Microbiology 2023Quote: ... Forty-three microliters of the cell suspension were applied in triplicate for each strain and allowed to dry in a MaxiSorp microtiter dishes (Nunc) plate overnight ...
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bioRxiv - Bioengineering 2023Quote: ... the bacterial toxin ccdB was cloned with the enzymes KpnI and NheI using a dedicated commercial ccdB-resistant strain (ThermoFisher); the generated plasmid LentiCRISPRV1-gRNA-TetR-intron-ccdB was used to obtain the KpnI/NheI digested backbone for lentiviral library ...
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bioRxiv - Microbiology 2023Quote: Two-day biofilm formation of Pseudomonas strains was determined by crystal violet staining using a 96-well plate lid with pegs extending into each well (Nunc-TSP lid ...
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bioRxiv - Plant Biology 2023Quote: ... All entry clones were propagated in the DH5 [strain of E.coli using a single gateway cloning system (Invitrogen, Cat# 12538120). The sense and antisense for STM were cloned under pG1090 inducible promoter (Zuo et al ...
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bioRxiv - Microbiology 2024Quote: Brachyspira pilosicoli strain P43/6/78 (ATCC #51139) was grown in Brain Heart Infusion (BHI) broth (Thermo Fisher Scientific, #CM1135B) containing 10% heat-inactivated fetal bovine serum and 0.2% glucose solution for four days in an anaerobic chamber (Coy Lab Products ...
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bioRxiv - Microbiology 2024Quote: ... and FCR-CD36 strains were maintained in culture under standard conditions in O+ human erythrocytes in RPMI 1640 containing L-glutamine (Invitrogen) supplemented with 5% Albumax I ...
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bioRxiv - Immunology 2024Quote: CD4+ T cells were derived from twenty-month old mice (C57BL/6 strain) and stimulated for 48 hours with CD3/CD28 Dynabeads (11452D, Gibco). The cells were then transduced with p-SIREN-sh lentivirus particles to target sestrins or with control p-SIREN scramble vectors ...
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bioRxiv - Microbiology 2024Quote: ... All ESKAPE strains were grown at 30-37°C using Luria broth/agar (BD Difco, Fisher Scientific #DF0145-17-0) for 15-24 h.
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bioRxiv - Cell Biology 2019Quote: ... protein was extracted using 0.2M Sodium Hydroxide and protein concentrations were determined using the Pierce™ 660 protein assay (Fisher Scientific), according to manufactures instructions ...
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bioRxiv - Pathology 2020Quote: ... Fractions were tested for protein concentration using 5 μL of protein sample mixed with 10 μL Coommassie Protein Assay reagent (Thermo Scientific). Pooled fractions were concentrated to 4 mL using 10 kD molecular weight cut-off spin columns (Millipore ...
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“A Proteogenomic workflow reveals distinct molecular phenotypes related to breast cancer appearance”bioRxiv - Systems Biology 2020Quote: Collected protein flow-throughs (FT) were subjected to protein precipitation for downstream protein content determination using bicinchoninic acid assay (BCA, Thermo Fisher) assay and trypsin protein digestion ...
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bioRxiv - Cell Biology 2021Quote: ... The protein concentrations of the lysates were measured by BCA protein assay using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) and then unified to 0.5 µg/µL ...
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bioRxiv - Biochemistry 2022Quote: Protein concentration after cell lysis and after protein precipitation was determined using the Micro BCA™ Protein assay kit (Thermo Scientific) according to manufacturer’s guidelines.
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bioRxiv - Neuroscience 2019Quote: ... Newly synthesized proteins were then enriched using the Click-it protein enrichment kit for chemistry capture of azide modified proteins (Invitrogen C10416) following the manufactures protocol with small modifications ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein expression was performed according to Culturex protocol while protein purification was done using Pierce Protein A Agarose kit (ThermoFisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... Measurement of final protein concentration was performed using BCA protein assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific™, #23227), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Soluble marker protein levels were normalized to total protein levels measured by Quant-iT protein Assay Kit (Thermo Fisher Scientific, USA).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... were supplemented with SDS to a final concentration of 2% and the protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific). Filter-aided sample preparation (FASP ...
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bioRxiv - Neuroscience 2022Quote: Recombinant NRX1β-Fc and NRX1βΔHRD-Fc proteins were pre-immobilized with Protein G Magnetic Beads (Dynabeads Protein G, Cat# 10004D, Thermo Fisher Scientific) in PBS overnight at 4 °C with constant agitation ...
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bioRxiv - Immunology 2024Quote: ... Protein concentrations across all samples were normalised to the same protein concentration (500 µg/ml) with protein determination quantified using the well-established Pierce BCA Protein Assay (Thermo Fisher) following the recommended guidelines provided by the manufacturer ...
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bioRxiv - Neuroscience 2023Quote: ... and supernatant removed for protein concentration analysis using a BCA protein assay (Pierce BCA Protein Assay Kit; ThermoFisher Scientific; cat # 23227). Twenty μg samples were loaded into SDS-PAGE gels and transferred onto Amersham Hybond-P 0.45μm PVDF membranes (GE Life Sciences ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysates were cleared by centrifugation and protein concentration was quantified using the Qubit™ Protein and Protein Broad Range Assay Kit (ThermoFisher). Protein samples were sent to the UNSW Bioanalytical Mass Spectrometry Facility for liquid chromatography-mass spectrometry (LC-MS/MS ...
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bioRxiv - Neuroscience 2023Quote: ... Fractions were tested for protein concentration using 5 μL of protein sample mixed with 10 μL Coomassie Protein Assay reagent (ThermoFisher Scientific). Pooled fractions were concentrated to 4 mL using 10 kDa molecular weight cut-off spin columns (Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... Total protein concentration in supernatant was determined using bicinchoninic acid (BCA) protein assay (PierceTM BCA Protein Assay Kit, Catalog #23225, ThermoFisher Scientific). 30-40 µg total protein sample was denatured at 75 °C for 7.5 min and then electrophoresed using polyacrylamide gel (4–15% tris-glycine extended polyacrylamide gel ...
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bioRxiv - Molecular Biology 2023Quote: ... Eluted proteins/complex were subject to SDS-PAGE electrophoresis followed by protein staining (SYPRO Ruby Protein Gel Stain, Thermo Fisher Scientific) and immunoblotting.
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bioRxiv - Physiology 2024Quote: ... The protein content of the EV preparations was quantified by bicinchoninic acid protein assay (Pierce™ BCA Protein Assay Kit, Thermofisher).
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bioRxiv - Developmental Biology 2024Quote: ... The purified proteins were stored at −80°C and the protein concentration was determined using Bradford plus protein assay reagents (23238, Thermo Scientific).
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bioRxiv - Neuroscience 2024Quote: ... and protein concentration in the lysates was measured using a bicinchoninic acid protein assay kit (Micro BCA Protein Assay Kit, Thermo Scientific). Equal amounts (750 micrograms to 1 mg depending on the experiment ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentrations were determined with the Pierce BCA Protein Assay Kit (Thermo Scientific), and extracts were loaded onto NuPAGE Bis–Tris SDS gels and immunoblots were visualized by LiCOR Odyssey system.
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentration was determined using the Pierce BCA Protein Assay Kit (ThermoFisher Scientific), and the pHrodo-TDM1 fluorescent signal was normalized to the amount of protein at each time point.
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bioRxiv - Cancer Biology 2021Quote: ... The total protein concentrations were determined using BCA protein assay kit (Thermo Scientific), and IFN-α and IFN-γ protein concentrations were measured using a human IFN-α ELISA kit (1110012 ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentration was determined using Pierce BCA protein assay kit (Thermo Fisher Scientific) and protein lysates were subjected to electrophoresis through SDS–PAGE or 4-12% NuPage Bis-Tris protein gels (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... protein concentration of the supernatant was determined using BCA protein assay (Thermo Fisher) and 50 μg (1C and S2F ...
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bioRxiv - Neuroscience 2020Quote: ... Total protein content was quantified using a BCA Protein Assay Kit (Thermo Scientific) and equal amounts of total protein were loaded onto 4 – 15% Tris-Glycine gels (BioRad) ...
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bioRxiv - Cell Biology 2019Quote: ... and protein concentrations were determined using the BCA protein assay (Thermo Fisher Scientific). Protein lysates were analyzed by SDS PAGE and Western blot using fluorescently-labeled secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were visualized using a reversible protein stain (Memcode, Pierce, Thermo Fisher Scientific) and membranes were incubated with the following primary antibodies listed in Table S3 ...
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bioRxiv - Cell Biology 2020Quote: ... The total protein content was measured using BCA Protein Assay Kit (ThermoFisher Scientific). The relative ALP activity was normalized to total protein content and expressed as units/g·protein.
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was measured using the Pierce BCA Protein Assay kit (Thermo Fisher). An equal volume of 2x Laemmli buffer (BIO-RAD ...
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bioRxiv - Cell Biology 2020Quote: ... and protein concentration was determined with the Pierce BCA protein assay kit (ThermoFisher). Insoluble materials were removed by centrifugation at 21,000 g for 30 mins at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were determined using a BCA protein assay kit (Thermo Fisher Scientific). Aliquots of lysates were solubilized in Laemmli sample buffer and equal amounts of proteins were separated on 12% SDS-PAGE gels ...