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Citations for Thermo Fisher :
2001 - 2050 of 6826 citations for Recombinant Human IL4 None tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Dynabeads conjugated with human anti-CD63 (10606D, Invitrogen), CD9 (10614D ...
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bioRxiv - Developmental Biology 2023Quote: ... Human iPSCs were maintained on Geltrex-coated (Invitrogen) plates in E8 media (STEMCELL Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... TaqManTM Human Neurotransmitter PCR Array (ThermoFisher Scientific, #4414094) was used to detect the expression of neurotransmitter-and GABA-related genes in GFP+ HCT116 harvested from the top epithelial channel of stretched and not stretched chips and sorted via flow cytometry ...
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bioRxiv - Biophysics 2024Quote: ... human embryonic kidney (HEK) 293A (Thermo Fisher Scientific) cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cell Biology 2024Quote: ... human Kif22/Kid siRNA (Ambion, #Cat 4392420, ID:s7911), human WAPL ON-TARGETplus SMART pool siRNA (J-026287-10-0010 ...
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bioRxiv - Cell Biology 2024Quote: ... and human OTC (Thermo Fisher Scientific, 4331182-Hs00166892_m1).
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bioRxiv - Cancer Biology 2024Quote: ... human EGF (Thermo Fisher Scientific, Cat. No. PHG6045) and Pen/Strep/Glutamine (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... anti-cGAS antibody (human or mouse-specific, Invitrogen). PLA signals were visualized using a Zeiss LSM 980 Airyscan 2 NLO Two-Photon Confocal Microscope and indicated by PLA red dots per cell.
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bioRxiv - Immunology 2024Quote: ... Monoclonal antibodies targeting Human IgA (Invitrogen, MA5-32575), IgG (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... and OxPhos Human WB antibody cocktail (Thermofisher 458199). For visualization ...
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bioRxiv - Cancer Biology 2024Quote: ... human bFGF (Thermo Fisher Scientific, Cat. No. PHG0261), human EGF (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... followed by globin reduction using GlobinClear Human (ThermoFisher). To generate sequencing libraries ...
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bioRxiv - Neuroscience 2024Quote: ... and a panel of ProcartaPlex Human Simplex (Invitrogen) beads ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 ng/mL human basic FGF (Gibco, PMG0035) and 25 ng/mL FGF10 (R&D ...
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bioRxiv - Cell Biology 2020Quote: HEK293T cells stably expressing a single copy of N-terminally tagged TTBK2- or GFP-BirA* were generated using Flp-IN T-REx cells (ThermoFisher K6500-01), Positive clones were selected with hygromycin resistance ...
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bioRxiv - Cell Biology 2020Quote: ... was used at a 1:1000 dilution overnight at 4°C followed by incubation with fluorescently tagged goat anti mouse-A647 and donkey anti-rabbit-A555 secondary antibodies at 1:100 dilution (Invitrogen, Carlsbad, CA). TUNEL staining to detect apoptotic cells was completed prior to antibody stainig per manufactuors instructions (Promega ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 WT cells and the UPF3 dKO clone 2 expressing either FLAG-tagged GST or UPF2 were labeled by culturing them for at least 5 passages in DMEM for SILAC medium (Thermo Fisher Scientific) supplemented with 9% FBS (Silantes) ...
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bioRxiv - Molecular Biology 2020Quote: HA-tagged ubiquitin (a gift of the F. Sicheri lab) was overexpressed in ES cells by transfection with Lipofectamine 2000 (Thermo Fisher Scientific), 500 ng per ∼8×106 cells in a 10 cm dish ...
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bioRxiv - Molecular Biology 2020Quote: ... the primary antibody was rinsed three times with PBS then Alexa Fluor 546-tagged goat anti-rabbit IgG secondary antibody (ThermoFisher, A-11035) was added at a 1:1250 dilution and incubated for 1 hour before a final 3x rinse with PBS.
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bioRxiv - Developmental Biology 2020Quote: ... The next day the antibody was washed five times in PBST 0.1% with gentle agitation at room temperature (30 minutes each) and fluorescently-tagged by horseradish peroxidase-catalysed signal amplification (Thermo Scientific, T20913)
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bioRxiv - Plant Biology 2020Quote: ... To create the 6xHis-tagged MBP fusion of full-length WUS the coding sequence was amplified with Phusion DNA Polymerase (Thermo Fisher Scientific), digested with NcoI/XhoI and ligated into pMG21053 previously digested with NcoI/SalI ...
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bioRxiv - Cell Biology 2020Quote: ... AHA-labeled proteins were detected with 1 μM alkyne-tagged Alexa Fluor-488 using Click-iT cell detection reagents (Thermo Fisher Scientific) for 45 min at room temperature as previously described (3).
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bioRxiv - Cell Biology 2020Quote: ... For transient overexpression (Fig 2 E-H, S2G) seipin tagged with C-terminal EGFP [34] was inserted into pcDNA5/FRT/TO (Thermo Fisher Scientific) through HindIII/NotI sites ...
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bioRxiv - Biochemistry 2020Quote: pcDNA5-based constructs for the expression of C-terminally His6-PreScission protease cleavage site-2xFlag (Flag)-tagged full-length or N-terminally truncated ASCC3 variants were transfected into HEK293 Flp-In™ T-REx™ cells (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: Bacterial expression constructs coding for His6/SUMO-tagged fusion proteins of rat Shank3 (residues 1-348, SPN-Ank) were generated in pET-SUMO (Thermo Fisher Scientific) as described before (17) ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were washed three times with washing buffer (PBS containing 0.7% Triton X-100) before incubation with secondary antibodies (tagged with Cy2, Cy3, or Cy5; dilution 1:500; Life Technologies Inc., USA) for 1 h at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... This was followed by staining with the corresponding fluorescent tagged secondary antibodies for 1 hour at room temperature: goat anti-rabbit Alexa Fluor 647 (Thermo Fisher Scientific), donkey anti-goat Alexa Fluor 488 (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... sections were rinsed in PBS and incubated in AlexaFluor-647-tagged goat anti-mouse IgG (1:100; Thermo Fisher Scientific, catalog #A21039), AlexaFluor-647-tagged donkey anti-mouse IgG ...
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bioRxiv - Microbiology 2022Quote: ... Cells were transfected with 2 μg of phCMV3 plasmids encoding the C-terminal HA-tagged NA from the strain of interest using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: The gene encoding N-terminal His-tagged V2HeR3 was cloned into the EcoRI and XbaI site of pPICZB vector (Thermo Fisher Scientific). The recombinant protein was expressed in the Pichia pastoris strain SMD1168H (Philosof et al. ...
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bioRxiv - Biophysics 2022Quote: ... the gene encoding N-terminal His-tagged V2HeR3 was cloned into the EcoRI and XbaI site of pPICZB vector (Thermo Fisher Scientific). For determination of λmax of V2HeR3 WT and mutants ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were washed three times with washing buffer (PBS containing 0.7% Triton X-100) before incubation with secondary antibodies (tagged with Cy2, Cy3, or Cy5; dilution 1:500; Life Technologies Inc., USA) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... E14.5 Apcdd1 mutant and wildtype control brains were cryosectioned at 16 microns and immunolabeled with Alexa488-tagged Isolectin (1:100; Thermo Fisher Scientific); Calbindin (1:1,1000 ...
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bioRxiv - Immunology 2020Quote: 293 ET cells were plated at 250,000 cells/well in six-well plates and a day later were transfected with 1 μg of pBent2-HA-IRF5 and 1 μg of plasmid encoding one of the myc-tagged candidate IRF5 kinases (in the pEAK8-myc vector) using Lipofectamine2000™ (Life technologies) according to the manufacturers protocol ...
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bioRxiv - Bioengineering 2021Quote: ... The amounts of peptides to be tagged were normalized and mixed with 41 μL of TMT reagent (TMT sixplex, Thermo Fisher Sci) freshly dissolved in acetonitrile (20 μg/μL ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed in 0.1 M PBS and incubated in Alexa Fluor 647-tagged donkey anti-rabbit IgG (1:500, ThermoFisher, cat# A-31573) for 2 hr at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... HeLa cells were transiently transfected with plasmids expressing FLAG-tagged version of BPLF1/BPLF1-C61A using the lipofectamine 2000 (Invitrogen, California, USA) or jetPEI® (Polyplus transfection ...
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bioRxiv - Genetics 2022Quote: ... FRAP was performed on 3T3-L1 preadipocytes transiently transfected with GFP-tagged CIDEC were incubated with 200 μM of oleic acid and stained with Bodipy 558/568 C12 fatty acid (Molecular Probes, D3835) for 15 hours ...
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bioRxiv - Biochemistry 2021Quote: ... 4 µl of resuspended MTG1-3xFLAG tagged mitoribosome sample was directly applied onto the grid mounted in the Vitrobot chamber (Thermo Fisher Scientific) and incubated for 1 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Co-purification of 6His-tagged PGC-1α protein domains was detected by western blot using anti-6His antibody (clone His.H8, ThermoFisher Scientific MA1-21315).
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bioRxiv - Neuroscience 2020Quote: ... or Alexa Fluor 594-tagged goat anti-guinea pig IgG (1:500, Thermo Fisher, cat# A-21202, A-21206 and A-11076) for 1.0 – 1.5 hours at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... and rabbit anti-D5R antibody and donkey anti-rabbit secondary antibody tagged with Alexa Fluor™ 488 (#R37118, ThermoFisher Scientific Waltham, MA). The tissues were counterstained with wheat germ agglutinin (WGA ...
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bioRxiv - Cell Biology 2021Quote: ... and anti-TRIP4 antibodies were raised in rabbits against affinity purified N-terminally hexahistidine-tagged full-length proteins and purified with the respective antigens coupled to SulfoLink resin (Thermo Fisher Scientific).
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bioRxiv - Immunology 2021Quote: ... 0.02% Triton X-100 and 200 μM ATP for 10 min at 30°C or with purified hexahistidine-tagged TBK1 (catalog # PV3504; Thermo Fisher Scientific) in buffer B containing 50 mM Tris [pH 7.5] ...
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bioRxiv - Microbiology 2021Quote: ... Purification of His-tagged Cg1978 was performed by applying the supernatant to an equilibrated 2 ml Ni-NTA column (Invitrogen, Kalifornien, USA). After washing with 30 ml TNI20 buffer ...
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bioRxiv - Cell Biology 2022Quote: ... SH-SY-5Y cells stably expressing GFP or a GFP-tagged construct of the protein of interest were cultured for at least six doublings in SILAC DMEM (89985; Thermo Fisher Scientific) supplemented with 10% dialyzed FBS (F0392 ...
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bioRxiv - Neuroscience 2022Quote: ... DNAJB4 or DNAJB6 constructs and 2) GFP-tagged polyQ or desmin constructs into C2C12 cells using Lipofectamine LTX and Plus reagent (Thermo Fisher Scientific). The rate of the cells with aggregate was observed three days post-transfection.
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bioRxiv - Plant Biology 2023Quote: ... constructs coding for mCherry tagged versions of the peptides were generated in pK7m34GW-rolD∷EGFP destination vector using Multisite Gateway Technology (Thermo Fisher Scientific). The entry clones contained the same promoter fragments in pDONRP4-P1R which were used for complementation experiments ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: His-tagged relaxins were expressed as secreted proteins in Expi293F cells containing a stably integrated tetracycline repressor (Expi293F tetR, Thermo Fisher Scientific) grown in Expi293 media (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... and then with buffer containing 250 nM purified His-tagged protein that was previously pre-clustered with anti-His (mouse; Thermo Fisher Scientific) in a 2:1 (protein:antibody ...