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Citations for Thermo Fisher :
2001 - 2050 of 7736 citations for Recombinant Human C1QB His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The lysate was then incubated with appropriate antibodies for 12 h and then conjugated with dynabeads Protein A or Protein G beads for an additional 1 h SFB- or GFP-tagged proteins were immunoprecipitated with streptavidin dynabeads (Invitrogen) or GFP-Trap (Chromotek) ...
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bioRxiv - Developmental Biology 2021Quote: ... The following primary antibodies were used: mouse anti-V5-tag to amplify V5-tagged CFP (Thermo Fisher, R96025, 1:650), rat anti-mCherry (Thermo Fisher ...
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bioRxiv - Neuroscience 2019Quote: ... Chicken anti-rabbit tagged with Alexa Fluor 488 and goat anti-mouse tagged with Alexa Fluor 568 were used as secondary antibody at a dilution of 1:1000 (Invitrogen). Tissues were incubated with these secondary antibodies for 48 hours at 4 °C followed by regular washing ...
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bioRxiv - Molecular Biology 2020Quote: ... MBP-tagged full-length TRIM21 was cloned into a pFastBac vector for expression in the baculovirus system (Bac-to-Bac, Invitrogen), resulting in pFastBac-MBP-TRIM21 ...
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bioRxiv - Molecular Biology 2020Quote: ... Large-scale expression of Strep-tagged proteins for purification was conducted in Trichoplusia Ni High Five cells (Thermo Fischer Scientific) in SF-900 medium (Gibco). Cells at a density of 2×106 cells/ml were infected with 1.5% of amplified virus and incubated for 40 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse IP6K1 was sub-cloned into SFB (S-protein/FLAG/SBP) - tagged destination vector using the Gateway cloning strategy (Invitrogen). Catalytically inactive IP6K1 (K226A ...
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bioRxiv - Molecular Biology 2019Quote: Expression constructs for repeat RNAs or GFP-tagged codon-optimized DPRs were transfected in HEK293 cells or U-2 OS cells with Lipofectamine 2000 (Invitrogen) or FuGENE HD (Promega) ...
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bioRxiv - Molecular Biology 2019Quote: ... with Strep and His epitope tags was amplified by PCR from pIEx10-LRIM1 (Povelones et al. 2009) with BamHI tagged primers (LRIM1-F, LRIM1-R) and subcloned into pJET1.2 (Thermo Scientific) before cloning into pslVg-BGH-attP ...
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bioRxiv - Molecular Biology 2019Quote: BGH was amplified by PCR from pslVg-GFP-BGH by PCR with BamHI and NotI tagged primers (BGH-F, BGH-R) and subcloned into pJET1.2 (Thermo Scientific). attP was amplified from pBCPB+ (Groth et al ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid encoding for FLAG-tagged TM or IM-TM were transfected into EXPI293F cells according to the manufacturer (Thermo Fisher), and incubated at 37°C ...
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bioRxiv - Neuroscience 2019Quote: ... and subsequently incubated in secondary antibody solution (AlexaFluor 488-tagged goat anti-rabbit Ab, 1:1000, ThermoFisher Scientific, A-11008) for 1 h at RT ...
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bioRxiv - Molecular Biology 2019Quote: T7-tagged cDNA template was PCR amplified and subjected to in vitro transcription using a MEGAscript T7 kit (Applied Biosystems). Amplified cRNA was purified with an RNeasy Mini Kit (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were next incubated in biotinylated secondary antibody for 1 hour at room temperature followed by incubation in fluorophore tagged horseradish peroxidase (Alexa Fluor; Thermofisher). Sections were washed thrice in PBS-T and counterstained with RNAscope DAPI for 30 seconds ...
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bioRxiv - Biochemistry 2020Quote: ... pcDNA5/FRT/TO plasmid encoding Flag-tagged WT ExoU or S142A ExoU was incubated in serum free medium containing lipofectamine 2000 (Invitrogen) for 30 minutes at ambient temperature ...
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bioRxiv - Biochemistry 2020Quote: MBP-tagged (pTHMT) full-length SARS-CoV2 nucleocapsid protein was expressed in Escherichia coli BL21 Star (DE3) cells (Life Technologies). Bacterial cultures were grown to an optical density of 0.7 to 0.9 before induction with 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were co-transfected with either a constant concentration of 0.1 μg of NF1-NanoLuc pcDNA expression plasmid or CRAF RBD-NanoLuc pcDNA expression plasmid with 0.2 μg of GFP-tagged KRAS (WT or Mutant) with 0.25 μl of Lipofectamine 2000 per well following the manufacturer’s protocol (Thermo Fisher Scientific). Twenty-four hours later ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transiently transfected with mCherry-tagged wild type or mutant ORP1L at 70% confluency using Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... The generation of HeLa stable cell lines expressing all different variants of FLAG-tagged HERPUD1 cloned in the pLVX-IRES-Puro vector were generated by transfection with Lipofectamine 2000 (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... Clarified lysates containing His6x-tagged proteins were subjected to gravity-flow Ni2+-affinity purification using HisPur Ni-NTA Resin (ThermoFisher) following manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2020Quote: ... LLC-luc cells were stably transfected with CIITA overexpression vector pcDNA3-myc-tagged CIITA (addgene Plasmid #14650) or control pcDNA3.1 (Thermofisher V79020) using Lipofectamine 2000 Reagent (Invitrogen 11668-027) ...
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bioRxiv - Microbiology 2021Quote: ... nucleotide sequences encoding N-terminal FLAG-tagged VACV proteins were subcloned between the BamHI and XbaI restriction sites of a pcDNA4/TO vector (Invitrogen). Alternatively ...
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bioRxiv - Molecular Biology 2020Quote: ... The Mex67-3Flag tagged protein was isolated using 200 µL of pre-washed magnetic Pan Mouse Dynabeads (Thermo Fisher Scientific) coupled to 34 µg of anti-Flag M2 mouse monoclonal antibody (Sigma) ...
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bioRxiv - Microbiology 2020Quote: Expression vectors for FixL and FixJ His6-tagged proteins were generated using the Gateway high-throughput recombinational cloning system (Invitrogen). The entire fixJ gene or fixL amino acids 329-851 (lacking transmembrane domains ...
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bioRxiv - Biophysics 2020Quote: ... C-terminal His6-tagged CLC-ec1 cloned in the pASK vector was transformed in E.coli BL21(DE3) (Invitrogen, Carlsbad, CA) and the expression of CLC-ec1 protein was induced with anhydrotetracycline (Acros Organics ...
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bioRxiv - Cell Biology 2022Quote: ... Fluorescently tagged G-actin was prepared by covalent modification with Alexa Fluor™ 594 Carboxylic Acid (Thermo Fisher Scientific 15461054) (Alvarado and Koenderink ...
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bioRxiv - Molecular Biology 2022Quote: ... from the 2xStrep-tagged or 3xFLAG-tagged plasmids with oligonucleotide primers containing attB recombination sites and recombined into pDONR221 using BP clonase II (ThermoFisher) (Supplementary Table 6) ...
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bioRxiv - Genetics 2022Quote: ZsGreen and flag-tagged ADAR1-p110 mRNA were made using the Invitrogen MEGAscript T7 Transcription Kit (Fisher Scientific, Cat #: AM1334) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... The HA-tagged proteins were captured with the Pierce™ HA-Tag Magnetic IP/Co-IP Kit (Thermo Scientific™). Magnetic beads (0.25 mg ...
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bioRxiv - Molecular Biology 2021Quote: ... Cockayne syndrome B [CSB] fibroblast SV40 immortalized) and CS + CSB (CS cells stably expressing GFP tagged CSB from a BAC) cells were cultured in DMEM (ThermoFisher) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... The entry clones were recombined into the Gateway binary vector pGWB552 tagged with G3 green fluorescent protein (G3GFP) using LR clonase (Invitrogen) (Nakagawa et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... the receptor Fc-tagged ectodomains present in the conditioned media were captured on protein A-coated 384-plates (Thermo Scientific), and stored at 4 °C until use ...
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bioRxiv - Molecular Biology 2020Quote: Stable cell lines expressing low levels of HA-tagged Gli2 and Gli3 variants were generated using the Flp-In system according to the manufacturer’s recommendations (Life Technologies) (22) ...
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bioRxiv - Plant Biology 2021Quote: Expression vectors for HA-tagged PpCSLDs under control of their native promoters were constructed using Gateway Multisite Pro cloning (Invitrogen). The CSLD2 and CSLD6 coding sequences were amplified from cDNA clones pdp04669 and pdp21814 (RIKEN BioResource Center ...
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bioRxiv - Molecular Biology 2022Quote: NiV prefusion glycoprotein and avi-tagged probes were expressed by transfection in 293 Freestyle (293F) cells (Thermo Fisher Scientific, MA) using Turbo293 transfection reagent (SPEED BioSystem ...
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bioRxiv - Cell Biology 2022Quote: Histidine-tagged Mindin was purified from conditioned media collected from CHO-Mindin cells using Ni-NTA beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... The resulting TGD057 HA-tagged DNA fragment was then cloned into the pTKO-att parasite expression vector (92) by Gateway Recombination Cloning Technology (Invitrogen). The resulting vector was linearized and transfected into RH Δhxgprt Δku80 parasites by electroporation in a 2 mm cuvette (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2020Quote: Bacterial expression constructs coding for His6/SUMO tagged fusion proteins of rat SHANK3 were generated in pET-SUMO (Thermo Scientific) as described 14 ...
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bioRxiv - Biochemistry 2020Quote: ... the clarified lysates from His-tagged or dual tagged constructs were loaded onto an Nickel-nitrilotriacetic acid (Ni2+-NTA; 1.6 mL suspended beads/L growth) column (Thermo Scientific) equilibrated with 10 column volumes (CVs ...
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bioRxiv - Molecular Biology 2019Quote: ... 6X N-terminally Myc-tagged cDNAs encoding Ku70 or Ku80 WT and mutants were cloned into pcDNA/FRT/TO (Invitrogen) and then transfected along with Flp recombinase expressing pOG44 plasmid using Lipofectamine transfection reagent (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... For Protein A-tagged strains, immunoprecipitation was carried out with Rabbit IgG-conjugated (MP-Biomedicals, SKU 085594) Dynabeads (Invitrogen, 14301) as described 57 ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue sections were washed thoroughly and then incubated with appropriate secondary antibodies tagged with A lexaFluor-488 or -594 (1:500; Invitrogen) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... Stably transfected HEK293 cells expressing N-terminally FLAG-tagged MOR or KOR were cultured in the presence of 250 μg/ml Geneticin (Gibco). For transient DNA expression ...
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bioRxiv - Microbiology 2020Quote: HEK293 were transfected with 3xFlag-tagged plasmids encoding each SARS-CoV-2 protein by Lipofectamine 3000 (Thermo Fisher Scientific, #L3000015). 3×107 HEK293 cells were harvested in 10 ml lysis buffer (50 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Microbiology 2021Quote: ... concentrated eluates issued from immunopurification of endogenous and RACK1-HA-tagged protein are separated on 10% Tris-glycine SDS-PAGE gels (Invitrogen), and stained with Imperial Protein Stain (Thermo Fisher) ...
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bioRxiv - Biophysics 2020Quote: ... HA or GFP-tagged DISP1 variants (anti-HA rabbit polyclonal, 1:1000 or anti-GFP rabbit polyclonal, 1:2000, Invitrogen), and β-actin (anti-β-actin rabbit polyclonal ...
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bioRxiv - Neuroscience 2022Quote: ... or isolated human synaptoneurosomes from AD brains (Tai et al., 2014; Tzioras et al., 2019) tagged with pHrodo Red succinimidyl ester (1:20, ThermoFisher) by media change ...
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bioRxiv - Genomics 2022Quote: A total of 6,114 animals from all year-classes evaluated in the sea lice challenges were pit-tagged and genotyped using custom ThermoFisher (Affymetrix) SNP arrays developed exclusively for AquaGen’s Atlantic salmon populations ...
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bioRxiv - Microbiology 2022Quote: ... for 1 hour at room temperature followed by additional 1 hour incubation with 1:200 dilution of fluorescence-tagged goat anti-mouse IgG Alexa Fluor 488 (Thermofisher) (Supplemental Table 5) ...
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bioRxiv - Neuroscience 2022Quote: ... we incubated them in secondary antibody solution (AlexaFluor 488-tagged goat anti-rabbit Ab, 1:1000, ThermoFisher Scientific, A-11008) for 1h at RT ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated in Alexa Fluor 647-tagged donkey antirabbit IgG (1:500, Thermo Fisher, catalog # A-31573, RRID: AB_2536183) for 24 hours at 4°C ...