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Citations for Thermo Fisher :
2001 - 2050 of 10000+ citations for Prostaglandin E2 PGE2 Multi Format ELISA Kit 1 Whole Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... of each extract were screened in 384 well plate format (20 µL assay volume) supported by liquid handling robots (Analytik Jena CyBio Well 96/384 CYBIO SW-CYBI, Thermo scientific Matrix Wellmate and Multidrop). Assay plates were inoculated with 20.000 CFUs/mL from overnight pre-cultures (100 mL flasks filled with 30 mL cation adjusted Mueller Hinton II Broth (MHIIB ...
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bioRxiv - Neuroscience 2022Quote: ... and hsa-miR-485-5p (catalog#17100, PM10837) or with 50 pmol per well (24-well format) of miRNA miRVana inhibitors (Ambion, Thermo Fisher Scientific, Waltham, MA, USA) as indicated ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were transfected for 48 hours with 25 pmol per well (24-well format) of miRNA mimic Pre-miR miRNA precursors (Ambion, Thermo Fisher Scientific, Waltham, MA, USA) as indicated ...
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bioRxiv - Physiology 2023Quote: ... 20-50 µg of protein lysates were loaded to 10% or 12% polyacrylamide Tris-glycine SDS gels or to large-format NuPAGE 4-12% gradient Bis-Tris gels (Thermo Fisher Scientific # WG1403BOX and # WG1402BOX). After separation by electrophoresis ...
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bioRxiv - Immunology 2022Quote: ... The supernatants of human CD19 CAR T cells or human TCR transfected CD8+ T cells were analyzed by IFNγ Human Uncoated Elisa Kit (Invitrogen, Thermo Fisher Scientific # 88-7316-88). Plates were read on BioRad plate reader at a wavelength 450 nm and subtracted by 595 nm.
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bioRxiv - Microbiology 2022Quote: ... We extracted the genomic DNA of ELISA-positive cattle using either the magLEAD 12gC or a MagMAX™ CORE Nucleic Acid Purification Kit (Thermo Fisher Scientific Inc., Waltham, USA) with an automated nucleic acid extraction system (KingFisher Duo Prime ...
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bioRxiv - Neuroscience 2023Quote: TNFα and IL-6 released in the microglia conditioned medium were quantified using mouse TNFα and IL-6 ELISA kits according to manufacturer’s instructions (Invitrogen, 88-7324 and 88-7064-22, respectively) and a SpectraMax i3x spectrophotometer (Molecular Devices) ...
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bioRxiv - Immunology 2020Quote: ... were co-cultured in 96 well well plates at 1:1 E:T ratio with Raji tumor targets pre-labeled with Vybrant DyeCycle Ruby Stain (ThermoFisher) and CellTracker Deep Red Dye (ThermoFisher) ...
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bioRxiv - Genomics 2019Quote: ... with 1% beta-mercaptoethanol for Smart-seq2 and 384-well plates containing 0.6 µl 1% NP40 (Thermo Fisher Scientific) for CEL-Seq2.
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bioRxiv - Microbiology 2020Quote: Cells listed in Sup.Table.1 were seeded at a density of 1×105 cells/well in a 24-well plate (Nunc) and 24h later infected with SARS-CoV-2 England-2/2020 ...
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bioRxiv - Immunology 2022Quote: ... and HFs were placed individually into a 96-well plate slot with 100 µl supplemented medium (William’s E plus 1% penicillin-streptomycin antibiotics, 1% L-glutamine (Invitrogen-Gibco), 0.01% hydrocortisone (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: Monolayer cultures of patient-derived GMB TPCs were propagated on Matrigel-coated plates in DMEM:F12 Neurobasal Medium (1:1; Gibco), 1% B27 supplement (Gibco) ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were trypsinized and transferred (1 200-1 800 cells/ well) to 96-well Nunclon Sphera plates (Thermo Scientific) for gene expression ...
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bioRxiv - Immunology 2020Quote: ... in quadruplet were prepared in 96-well round-bottom plates using Dulbecco’s Modified Eagle Medium (DMEM) with 1% (v/v) FBS and 1% Antibiotic-Antimycotic (Gibco). 75 µL of the diluted sera was mixed with an equal volume of media containing 64 PFU of SARS-CoV-2 virus (hCoV-19/England/02/2020 ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were seeded on 0.1% gelatin-coated plates and NPCs are cultured in N2B27 medium (1:1, Neurobasal:DMEM-F12, supplemented with N2 and B27, ThermoFisher) in the presence of 10 ng/ml bFGF (Peprotech ...
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bioRxiv - Bioengineering 2023Quote: ... while non-BrdU plates were stained with calcein AM and ethidium homodimer-1 Live/Dead stains (Invitrogen, 1:1000) to quantify living and dead cells ...
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bioRxiv - Cell Biology 2024Quote: ... mES cells were cultured on 0.2% gelatin coated plates in 2i/LIF containing medium (1:1 mix of DMEM/F12 (Givco) and Neurobasal (Gibco) supplemented with 1x Pen-Strep (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... coverslips with the gel attached were transferred to a well of a 6-well plate with 1 ml of digestion solution (0.5% Triton X100, 1 mM EDTA pH 8 (AM9260G, Ambion), 50 mM Tris pH 8 (AM9855G ...
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bioRxiv - Biochemistry 2023Quote: ... mES cells were cultured on 0.2% gelatin coated plates in 2i/LIF containing medium (1:1 mix of DMEM/F12 (Gibco) and Neurobasal (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... coverslips with the gel stuck to it were transferred to a 6-well plate with 1 ml of digestion solution (0.5 % Triton X100, 1 mM EDTA, pH 8 (AM9260G, Ambion), 50 mM Tris ...
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bioRxiv - Cell Biology 2020Quote: ... MEF plates were used within 1 week and washed with DPBS (ThermoFisher Scientific 14190250) prior to plating of ESC/D-EPSCs in appropriate media.
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bioRxiv - Immunology 2021Quote: ... plates were washed and developed with streptavidin-HRP (1:1000; ThermoFisher Scientific, Pittsburgh, PA) and TMB ...
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bioRxiv - Microbiology 2020Quote: ... plating on BHI agar plates containing 10 μg mL−1 erythromycin (Erm; Fisher Scientific). A gene SOEing product merging the two flanking regions of relP was then generated ...
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bioRxiv - Immunology 2020Quote: ... cells were stimulated with 1 µg/mL plate bound anti-CD3 (UCHT1, Thermo Fisher Scientific Cat# 16-0038-85 ...
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bioRxiv - Genomics 2020Quote: ... cells were lysed directly in the plate by adding 1 ml of Trizol (Invitrogen). RNA was isolated using the Direct-Zol RNA Miniprep Kit (Zymo Research ...
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bioRxiv - Neuroscience 2020Quote: ... the plates were blocked for 1 h at room temperature using StartingBlock (Thermo scientific) to avoid non-specific binding ...
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bioRxiv - Systems Biology 2020Quote: ... These were captured in a 1 mL 96-well plate (Thermo Scientific AB-1127) and the volume was dried down in a Centrivap speedvac (Model 7810016) ...
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bioRxiv - Immunology 2022Quote: ... or 96-well (1 × 105 cells/well) plates in RPMI (Thermo Fisher, Waltham, MA) supplemented with 10% FBS and 1% Pen/Strep and incubated overnight ...
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bioRxiv - Microbiology 2022Quote: ... Plates were incubated for 1 hour with 100 μg/ml proteinase K (Thermo Scientific) in pH 5.0 sodium acetate buffer ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Plates were washed three times in PBS before adding 1:5000 Hoechst 33342 (Invitrogen). After a final three washes in PBS ...
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Bat influenza vectored NS1-truncated live vaccine protects pigs against heterologous virus challengebioRxiv - Microbiology 2020Quote: ... plates were added with 50 μl of HRP-conjugated Streptavidin (diluted 1:2000, Invitrogen) and incubated for 1h at room temperature ...
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bioRxiv - Immunology 2021Quote: ... plates were blocked with Blocker Casein (1% w/v) in PBS (Thermo Fisher Scientific) supplemented with 0.05% Tween 20 ...
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bioRxiv - Immunology 2022Quote: T cells were cultured in 48-well plates containing 1 ml RPMI 1640 (Invitrogen) with 10% fetal calf serum (Omega Scientific ...
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bioRxiv - Immunology 2022Quote: ... Plates were subsequently blocked with Blocker Casein (1%) in PBS (Thermo Fisher Scientific, 37528) supplemented with 0.05% Tween 20 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... in 6 well-plates in 1 ml of medium 50% minimal essential medium (Gibco), 25% heat inactivated horse serum (Gibco) ...
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bioRxiv - Biochemistry 2023Quote: ... Quenched sample aliquots were incubated for 1 hour in streptavidin coated plates (ThermoFisher Scientific) in Tris-buffered saline with 0.05% Tween-20 (TBS-T ...
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bioRxiv - Cancer Biology 2023Quote: ... Plates of non-CLL entities were additionally stained with Calcein AM (1 μM, Invitrogen). Three positions per well with a stack of ten images at a distance of 1.2 μm were acquired with a 40x water objective in confocal mode.
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bioRxiv - Immunology 2023Quote: ... Plates were subsequently blocked with Blocker Casein (1%) in PBS (Thermo Fisher Scientific, 37528) supplemented with 0.05% Tween 20 (Sigma Aldrich ...
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bioRxiv - Immunology 2023Quote: ... 1 × 104 Tfr cells were cultured for 12 hours in 96-well plates (Nunc) in complete RPMI 1640 (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 1 × 105 cells were seeded in a 6-well plate (140675, Thermo Fisher Scientific) in which glass coverslips coated with collagen IV (Nitta Gelatin ...
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bioRxiv - Cancer Biology 2023Quote: ... Plates were then sequentially incubated with goat-anti mouse biotin IgG (Invitrogen, 1:1000) and streptavidin-HRP (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were seeded on the plate in mediums containing 1/10.000 Hoechst 33342 (Invitrogen) and either the drug vehicle (3.5 µL DMSO/1 mL of medium ...
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bioRxiv - Immunology 2019Quote: ... Hemocytes were released in multi-well dishes ex vivo and Click-IT EdU detection was performed according to the manufacturer’s instructions (Invitrogen).
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bioRxiv - Microbiology 2021Quote: ... images were corrected for chromatic aberration as previously described57 using a sample of 0.1 µm TetraSpeck multi-colour fluorescent beads (ThermoFisher) adhered to glass as a reference sample ...
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bioRxiv - Developmental Biology 2020Quote: ... Brain samples were collected in 24 multi-well dishes and then mounted onto Superfrost Glass Slides (Thermo Fisher Scientific), all other samples were directly mounted on glass slides.
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bioRxiv - Developmental Biology 2022Quote: ... was added for 10-15 minutes on a rotary machine (PTR-35 360 vertical multi-function rotator, Thermofisher Scientific). Oocytes were then washed for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: Samples were heated to 95°C for 5min then separated alongside a spectra multi-color Ladder (Thermo Fisher 26634) through a 4-15% Tris-Glycine extended Stain-Free gel (Bio-Rad 5678085) ...
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bioRxiv - Cancer Biology 2023Quote: ... After sufficient color development read-out was performed at OD490nm (Multi-Scan Sky of Thermo Scientific Waltham, MA, USA). Cell viability was calculated by subtracting OD490nm of the dead control of each value (7.5 % ‘dead mix’ ...
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bioRxiv - Cancer Biology 2023Quote: ... synthetic multi-guide sgRNA sequences and purified Cas9 nuclease) and Lipofectamine CRISPRMAX Cas9 Plus transfection reagent (Thermo Fisher, CMAX00008) according to the protocol (entitled “CRISPR editing of immortalized cell lines with RNPs using lipofection” ...
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bioRxiv - Neuroscience 2023Quote: ... These sites were then utilized to clone the reporter construct into the pCDNA5 FRT multi-cloning site (ThermoFisher, V601020).