Labshake search
Citations for Thermo Fisher :
2001 - 2050 of 10000+ citations for Human β Amyloid 1 42 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2022Quote: ... In the analysis of the Woroniecka Human Diabetic Kidney Disease dataset (2011, Affymetrix Human Genome U133A 2.0 Array platform) 33 and the European renal cDNA bank (ERCB ...
-
bioRxiv - Neuroscience 2022Quote: Human embryonic kidney (HEK293T) and human neuroblastoma (SH-SY5Y) cells from ATCC were cultured in high glucose DMEM (Invitrogen) and DMEM-F12 (Invitrogen) ...
-
bioRxiv - Cell Biology 2023Quote: ... siRNA targeting human UBE3A or human MASTL (Integrated DNA Technologies) was transfected into cells using Lipofectamine RNAi MAX (Invitrogen). A non-targeting control siRNA was used as a control ...
-
bioRxiv - Biophysics 2023Quote: N-terminally 6xHis-tagged human Gβ1 (S2-N340) and human Gγ2 (M1-C68) were synthesized and separately cloned into pFastBac1 (Thermo Fisher) and expressed as described above ...
-
bioRxiv - Cell Biology 2023Quote: Human ApoE protein was quantified using the Invitrogen Human ApoE ELISA following the manufacturers guidelines (Invitrogen. Cat no. EHAPOE).
-
bioRxiv - Cell Biology 2023Quote: Human umbilical vein endothelial cells (HUVECs) and human coronary artery smooth muscle cells (HCASMCs) were purchased from Thermo Fisher Scientific (Waltham ...
-
bioRxiv - Molecular Biology 2023Quote: ... human breast cancer MCF7 cells and human bone osteosarcoma U2OS cells were in cultured in DMEM medium (Life Technologies) supplemented with 10% (v/v ...
-
bioRxiv - Molecular Biology 2023Quote: ... Hep-3B human hepatocellular carcinoma cells and MCF7 human mammary carcinoma cells were cultured in Eagle’s Minimum Essential Medium (EMEM) (Gibco) supplemented with 10% heat-inactivated and charcoal stripped fetal calf serum and antibiotics ...
-
bioRxiv - Cancer Biology 2023Quote: ... Normal human melanocytes were grown in Melanocyte Growth Media 254 supplemented with Human Melanocyte Growth Supplement-2 (Life Technologies), calcium chloride (0.3 μM) ...
-
bioRxiv - Immunology 2023Quote: ... they were incubated for 10 min at room temperature with a combined Fc block solution (2μL Human Fc block [BD Biosciences, San Jose, CA, USA] + 3μL heat-inactivated human AB serum [Fisher Scientific, Waltham ...
-
bioRxiv - Immunology 2024Quote: ... corresponding to the concentration of the highest standard of recombinant human IFNγ (Human IFNγ Gamma Uncoated ELISA kit, Invitrogen). IFNγ concentrations below the level of detection by the ELISA standard curve were set to 0 pg/ml ...
-
bioRxiv - Neuroscience 2021Quote: ... dual-SMAD inhibition was initiated by inhibiting BMP4 and TGF-β in knockout serum replacement (KSR; Knockout DMEM [Gibco], 15% KO serum replacement [Gibco], 1% non-essential amino acids (PAA), 2mM Glutamax [Gibco] ...
-
bioRxiv - Pathology 2021Quote: ... Larvae were injected in the common cardinal vein with 1 nl of Cy3-β-Lactoglobulin (5 mg/ml) and/or Alexa fluor™ 488 10 kDa dextran (D22910, Thermo Fisher, at 2 mg/ml) diluted in sterile H2O and successful injections were screened for on a Leica Mz10F fluorescent stereomicroscope ...
-
bioRxiv - Neuroscience 2023Quote: ... dual-SMAD inhibition was initiated by inhibiting BMP4 and TGF-β in knockout serum replacement (KSR; Knockout DMEM [Gibco], 15% KO serum replacement [Gibco], 1% non-essential amino acids (PAA), 2 mm Glutamax [Gibco] ...
-
bioRxiv - Immunology 2020Quote: ... followed by permeabilization using 1X permeabilization buffer (eBioscience) for 40 minutes at room temperature in the presence of the following intracellular antibodies: FoxP3 APC (clone PCH101, Invitrogen, cat# 17-4776-42, dilution 4:100); Tbet BV605 (clone 4B10 ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The T cells were isolated using the EasySep human T cell isolation kit (Stemcell) and activated with human T-activator CD3/CD28 Dynabeads (Gibco) at a bead:cell ratio of 1:1 ...
-
bioRxiv - Microbiology 2021Quote: ... which is composed of two ACE2 ectodomains linked to the Fc portion of the human IgG (Anand et al., 2020).Alexa Fluor-647-conjugated goat anti-human Abs (Invitrogen) were used as secondary antibodies to detect ACE2-Fc and plasma binding in flow cytometry experiments.
-
bioRxiv - Molecular Biology 2022Quote: In vitro sumoylation reactions were performed on protein array slides with more than 9000 human proteins spotted (ProtoArray® Human Protein Microarray v5.0, Invitrogen). The reaction was performed by the technical service of Invitrogen with our purified enzymes ...
-
bioRxiv - Immunology 2022Quote: ... For Cytokine Multiplex of Human Inflammation Panel (Invitrogen™ Inflammation 20-Plex Human ProcartaPlex™ Panel) (Catalog # EXP20012185901, eBioscience/Invitrogen) procedures were performed following manufacturer protocol and the plate was processed on BioRad Bio-Plex 200 system with Bio-Plex-HTF attachment (Bio-Rad ...
-
bioRxiv - Cancer Biology 2022Quote: RNA was isolated from human NSCLC cells and human clinical NSCLC tumor and normal samples by homogenization in Trizol reagent (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... Enzyme-linked immunosorbent assays (ELISAs) for human IL-8 were performed using the Human IL-8 ELISA Kit (ThermoFisher SCIENTIFIC) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2019Quote: HMF and MRC5 fibroblasts were transfected with a pool of 3 siRNAs targeting each of the 710 human kinases (Silencer Human Kinase siRNA Library, #A30079 Thermofisher). Fibroblasts (1250 cells/well ...
-
bioRxiv - Immunology 2019Quote: ... human PBMC derived T cells were purified by Human T cell isolation kit from Invitrogen (Invitrogen Dynal AS, Oslo, Norway) according to manufacturer’s instruction ...
-
bioRxiv - Immunology 2021Quote: ... and total T cells were purified from PBMC suspensions by negative immunomagnetic selection (purity >90%) using the Human Myeloid DC Enrichment Kit (STEMCELL) and the Untouched total human T cell (Invitrogen) kits ...
-
bioRxiv - Cancer Biology 2022Quote: ... primary human breast CAFs were transfected with 75 nm human periostin-targeting stealth siRNA (si-POSTN) (ThermoFisher siRNA ID: HSS116400) or 75 nm non-targeting control siRNA (si-Control ...
-
bioRxiv - Cell Biology 2022Quote: ... were prepared from fresh human umbilical veins as previously described (Barbieri et al., 1981) and maintained in Human Endothelial SFM (Gibco™ ...
-
bioRxiv - Microbiology 2022Quote: ... Surface PCDH1 was stained using human anti-EC7 mAb-3677 (5 µg/mL) followed by anti-human Alexa FluorTM 555 (ThermoFisher) for 1 h at 4°C each ...
-
bioRxiv - Microbiology 2022Quote: ... encoding full-length human MCT1 (uniprot ID: P53985) and human basigin (uniprot ID: P35613-2) were cloned individually into pFastBac vector (Invitrogen) for recombinant expression in Sf9 cells ...
-
bioRxiv - Molecular Biology 2023Quote: ... human ETAR (residues 21-406) and human ETBR (residues 27-424) DNA was cloned into a modified pFastBac vector (Invitrogen), which contains an N-terminal FLAG tag (DYKDDDD ...
-
bioRxiv - Neuroscience 2023Quote: ... The human FUS or human TARDBP cDNA was subcloned into the pENTR™/D-TOPO® vector (Thermo Fisher Scientific). To generate the Gateway destination vector pUAST-DEST ...
-
bioRxiv - Cell Biology 2023Quote: Human islets were transfected with 60 nM siRNA targeting human CEBPG (s2901, Silencer Select Pre-designed siRNA, Ambion, Life Technologies), TMEM176A (s226845 ...
-
bioRxiv - Cell Biology 2023Quote: Human islets were transfected with 60 nM siRNA targeting human CEBPG (s2901, Silencer Select Pre-designed siRNA, Ambion, Life Technologies), TMEM176A (s226845 ...
-
bioRxiv - Neuroscience 2023Quote: ... and secreted signaling proteins were measured in culture supernatants by the Human Immune Monitoring Center (HIMC) at Stanford University using a Human 48-plex Luminex Procarta Immuno-assay (ThermoFisher).
-
bioRxiv - Neuroscience 2023Quote: Human colorectal adenocarcinoma (ATCC, #SK809) and human pancreatic carcinoma (ATCC, #CRL1469) cells were grown in McCoy’s 5A medium (Gibco, #16600082) and Dulbecco’s Modified Eagle’s medium (Gibco ...
-
bioRxiv - Molecular Biology 2021Quote: ... Secondary antibodies were either AF647-conjugated goat anti-mouse IgG for β-actin (Invitrogen, A32728) or AF488 conjugated donkey anti-rabbit (Invitrogen ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... The probes for mouse TNF-α (Mm00443260_g1) and mouse β-actin (Mm00607939_s1) (Thermo Fisher Scientific) were used ...
-
bioRxiv - Microbiology 2019Quote: ... 100 mM β-mercaptoethanol) before separating on 4-12 % Bis-Tris polyacrylamide gradient gel (Invitrogen). For immunoblot analysis of THP-1 cells ...
-
bioRxiv - Developmental Biology 2022Quote: ... 15 ng/ml TGF β and 15 ng/ml TGF α (both Gibco, ThermoFisher Scientific) for 96h ...
-
bioRxiv - Developmental Biology 2022Quote: ... 15 ng/ml TGF β and 15 ng/ml TGF α (both Gibco, ThermoFisher Scientific) for 96h ...
-
bioRxiv - Biophysics 2021Quote: ... A Methyl-β-Cyclodextrin solution (200 mM) in Live Cell Imaging Solution (Thermo Fisher Scientific) was added to the cholesterol lipid film at a molar ratio of 10 to 1 ...
-
bioRxiv - Molecular Biology 2021Quote: ... β-catenin (CTNNB1) probes were used in order to determine gene expression (Thermo Fisher Scientific). Samples were run in duplicates in ViiA™ 7 Real-Time PCR System (Thermo Fisher Scientific) ...
-
bioRxiv - Pathology 2021Quote: ... The constructs of β-estradiol:avrE/y1/y2/yy:VFP were cloned by gateway cloning (Life Technologies). pBBR1:avrF:avrE-yy:HA was constructed by RsrII sub-cloning of the fragment containing yy mutation from pENTR/D-TOPO ...
-
bioRxiv - Biochemistry 2022Quote: ... 25mM imidazole and 5mM β-ME) with Pierce Protease Inhibitor Tablets (Thermo Fisher Scientific, USA) and 1 mM PMSF ...
-
bioRxiv - Neuroscience 2022Quote: ... 2-hydroxypropyl-β-cyclodextrin powder (HPβCD) was purchased from Acros Organics (ThermoFisher, Waltham, MA, USA). Vorinostat was dissolved in 100 mM HPβCD by boiling for 5 minutes ...
-
bioRxiv - Neuroscience 2022Quote: ... and Taqman probes for POLDIP2 (Hs00210257_m1) and the housekeeping gene β-actin (Hs99999903_m1) (Thermo Fisher). RT-qPCR was performed on the 7500 Fast or StepOnePlus Real-Time PCR System (Thermo Fisher) ...
-
bioRxiv - Physiology 2022Quote: ... 5 mM β-mercaptoethanol) supplemented with EDTA-free phosphatase and protease inhibitor cocktail (Life technologies) and lysed by Dounce homogenization (20 strokes) ...
-
bioRxiv - Molecular Biology 2022Quote: ... containing β-mercaptoethanol for 10 min before running on an SDS-PAGE gel (Invitrogen XP10205BOX). Gels were transferred onto nitrocellulose membranes using the iBlot 2 Dry Blotting System (Invitrogen IB23002 ...
-
bioRxiv - Developmental Biology 2023Quote: ... Reporter expression was assessed using the mammalian β-galactosidase assay kit (Pierce Technology, Thermo Scientific). Reporter expression in the presence of transcription factors was normalized to reporter expression in the presence of empty expression vector ...
-
bioRxiv - Microbiology 2023Quote: ... 20% β-mercaptoethanol) in preparation for SDS-PAGE using 12% Bis-Tris polyacrylamide gels (Invitrogen) and transferred to polyvinylidene difluoride membranes ...
-
bioRxiv - Microbiology 2023Quote: ... 100 mM β-mercaptoethanol) before separating on 4-12 % Bis-Tris polyacrylamide gradient gel (Invitrogen). After PAGE ...