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Citations for Thermo Fisher :
2001 - 2050 of 10000+ citations for 7 Methyl 1 2 3 4 tetrahydroisoquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... ProLong® Gold antifade reagent with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen #P36931) was added to each slide ...
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bioRxiv - Microbiology 2024Quote: ... for 2 h at 4°C and processed through Zeba spin desalting columns (ThermoFisher) to remove excess unbound biotin ...
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bioRxiv - Biochemistry 2024Quote: ... cells were stained with 300 nM 4’,6-diamidino-2-phenylindole (DAPI; D1306, Invitrogen) for 5 min at RT ...
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bioRxiv - Immunology 2022Quote: ... Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific, #D1306), and images were captured with LSM 880 confocal (Carl Zeiss ...
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bioRxiv - Microbiology 2023Quote: ... and nuclei were detected using 300 nM 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen), with washes in PBS ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The fluorescent nuclear stain 4’,6-diamidino-2-phenylindole (DAPI) was also from ThermoFisher.
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bioRxiv - Bioengineering 2023Quote: ... and DAPI (4′,6-diamidino-2-phenylindole) lactate salt were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4°C with 2 µg/ml CD40 protein (ThermoFisher, A42565) in fresh PBS ...
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bioRxiv - Genomics 2022Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) (0.1 mg/mL in PBS, Invitrogen, cat. D1306) was used to visualize nuclei ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were stained with DAPI (4′,6-diamidino-2-phenylindole; D1306, Thermo Fisher Scientific) prior to mounting (10-15 min at RT) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2-4 μg of plasmid was mixed with 500 μL Opti-Mem (Gibco 31985070), after which 2.5 μL Plus Reagent and 8 μL Lipofectamine LTX (Invitrogen 15338100 ...
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bioRxiv - Microbiology 2023Quote: ... Coverslips were mounted using SlowFade Gold with 4′,6′-diamidino-2-phenylindole (DAPI) (Invitrogen). Samples were analysed with an inverted Olympus IX81 widefield microscope ...
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bioRxiv - Bioengineering 2023Quote: ... samples were counterstained in 300 nM DAPI (4’,6-diamidino-2-phenylindole; D1306, ThermoFisher) diluted in blocking buffer for 2 hr at room temperature ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed in 4% formaldehyde containing 2 mg/mL DAPI nuclear stain (Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... samples were counterstained in 300 nM DAPI (4’,6-diamidino-2-phenylindole; D1306, ThermoFisher), 20 μg ml-1 AlexaFluor 488-labeled peanut (Arachis hypogaea ...
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bioRxiv - Developmental Biology 2024Quote: ... sperm were loaded with 2 mM of Fluo-4 AM (Invitrogen, Carlsbad, California, USA) diluted in 10% (w/v ...
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bioRxiv - Developmental Biology 2024Quote: ... The slides were stained with 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific) and mounted ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were counterstained in 300 nM DAPI (4’,6-diamidino-2-phenylindole; D1306, ThermoFisher), and/or 20 μg ml-1 AlexaFluor 488-labeled peanut (Arachis hypogaea ...
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bioRxiv - Microbiology 2024Quote: ... Cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (ThermoFisher Scientific, 62248).
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bioRxiv - Molecular Biology 2024Quote: ... 2 mL lysates from each sample were electrophoresed on 4-12% NuPAGE gels (Invitrogen)38.
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bioRxiv - Bioengineering 2023Quote: ... 4 µM ethidium homodimer-1 and 1 µg ml-1 Hoechst 33342 (Thermofisher, 62249) was added to chondrocyte-laden alginate hydrogels and was incubated for one hour ...
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bioRxiv - Microbiology 2021Quote: ... ATPase activity was assayed by a continuous spectrophotometric method using a 2-amino-6-mercapto-7-methylpurine ribonucleoside–purine nucleoside phosphorylase reaction to detect released inorganic phosphate (EnzChek kit; Thermo Fisher Scientific) (62) ...
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bioRxiv - Molecular Biology 2022Quote: ... HCA-7 and MDCK cells were allowed to differentiate for 7 days after plating at 100% confluency in the presence of 2 µg/mL doxycycline (Fisher Scientific AAJ6042203) when inducing transgene and/or reporter transcript expression ...
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bioRxiv - Microbiology 2021Quote: PBMCs were plated into a 10 cm dish at 7×107 cells/dish and adhered for 2 hours in AIM-V (Gibco-BRL, CA). The nonadherent cells were removed gently and frozen as the source of lymphocytes for co-culture use ...
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bioRxiv - Systems Biology 2019Quote: Determination of intracellular levels of total ROS was carried out by flow cytometry using 2’,7’-dichlorodihydrofluorescein diacetate (DCH2FDA, Thermo Fisher Scientific), as previously described (Amara et al ...
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bioRxiv - Microbiology 2020Quote: Amount of ROS in dissected guts (from 10 animals) was estimated using 2’,7’-Dichlorodihydrofluorescein diacetate (H2DCFDA, C6827, ThermoFisher Scientific, MA, USA), following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: The compound 5-(and-6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA, Image-iT LIVE Green Reactive Oxygen Species Detection Kit, Molecular Probes; Invitrogen, I36007) was used to visualise the in vivo production of ROS ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then washed three times with unsupplemented neurobasal media and loaded with 200μM 2’,7’-dichlorofluorescein diacetate (DCFH-DA) fluorescent dye (Thermo Fisher Scientific, MA) for 30 minutes at 37°C ...
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bioRxiv - Plant Biology 2022Quote: ... Mock-treated and infected roots of WT and HvMPK3 KO plants were incubated in 30 μM 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA; Cat no. C6827, Invitrogen™, USA) and PI (1 mg.ml-1 ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to nitrocellulose membrane at 20 V for 7 minutes using the iBlot™ 2 Transfer Stack (Thermo Fisher Scientific). Membranes were blocked in 5% non-fat milk powder in TBS+0.1% Tween (TBST ...
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bioRxiv - Neuroscience 2023Quote: Larval zebrafish at 4–7 days post-fertilization were anesthetized (0.025% MS-222, Syndel) and mounted in 2% low melting point agarose (Thermo Fisher Scientific 16520), dorsal-up ...
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bioRxiv - Microbiology 2023Quote: ... Cell pellets were washed and re-suspended in PBS buffer free of KCl and incubated in the dark with 25-μM of 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) suspended in dimethylsulfoxide (DMSO) for 60 min at room temperature (Molecular Probes, Invitrogen). After incubation ...
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bioRxiv - Bioengineering 2022Quote: The level of reactive oxygen species was assessed in the homogenate supernatant of RPE and retina tissue with using 2′–7′-dichlorofluorescein diacetate (DCFH-DA; ThermoFisher Scientific Inc.) molecular probes ...
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bioRxiv - Cancer Biology 2024Quote: ... Real-time PCR analysis was performed using Green PCR Kit (TransGen, Cat# 2-21) on a QuantStudio 7 Flex system (Thermo fisher, USA). The gene-specific primers were listed in Supplementary Table 2 ...
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bioRxiv - Immunology 2021Quote: ... and 1% HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid; Gibco). Wild type SARS-CoV-2 (isolate USA-WA1/2020) ...
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... 2 μL forward primer and 2 μL reverse primer (Invitrogen; Table 1), 14.2 μL water (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... and 1% HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid; Gibco). Wild type SARS-CoV-2 (isolate USA-WA1/2020 ...
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bioRxiv - Cancer Biology 2021Quote: Samples were boiled for 5 min prior to loading 7 µg of samples on to 4-15% SDS-PAGE gels alongside a molecular weight ladder (Thermo Fisher Scientific, cat# LC5602) and electrophoresis at 200V for 40 min ...
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bioRxiv - Neuroscience 2020Quote: ... Third instar larvae were dissected with cold HL3 and immediately fixed with PFA (4%) and incubated overnight at 4 C with primary antibodies (rabbit anti-Dlg, 1:1000; anti-Brp 1:100, Life Technologies). Alexa-conjugated secondary antibodies were used for secondary staining (Jackson Laboratories 1:500) ...
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bioRxiv - Immunology 2020Quote: PBMC (1-2*10^6 cells) and pDC (1-4*10^4) were maintained for 16 h in RPMI1640+10% FBS+ 1% (PS) (Gibco Laboratories) in 96 well round bottom plates ...
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bioRxiv - Immunology 2023Quote: ... Drosophila S2 cells were mixed with Ramos cells or B1-8hi B cells at a ratio of 1:4 (4×105 B cells and 1×105 S2 cells) followed by total RNA extraction with TRIzol reagent (Thermo Fisher), DNaseI digestion ...
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bioRxiv - Cell Biology 2022Quote: ... The medium was replaced at day 1 to remove non-adherent cells and afterwards every 2-3 day with DMEM/10% FBS/1% penicillin streptomycin (PS) (Gibco, USA, catalog number 15140-122). These cells were passaged serially when cells reached to 80-100 % confluency or when proliferation essentially ceased by using 0.25% Trypsin/EDTA (Gibco ...
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bioRxiv - Immunology 2023Quote: ... Purified T cells were activated for 1 to 3 days with Human T-Activator anti-CD3/CD28 dynabeads at a 1:2 bead-to-cell ratio (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). Prior to analyses ...
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bioRxiv - Cancer Biology 2023Quote: ... Patient-derived primary cell line GCDX62 was maintained in a 3:1 mixture of Keratinocyte-SFM (KSF; Thermo Fisher Scientific; supplemented with 2% (v/v) FCS ...
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bioRxiv - Cancer Biology 2024Quote: ... Firstly proteins were precipitated with 50 μL of acetonitrile followed by steroid extraction via liquid-liquid extraction with 1 mL tert-methyl butyl ether (MTBE, Acros Organics, Fisher Scientific, UK). The MTBE layer was then removed ...
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bioRxiv - Genetics 2020Quote: ... Cells were seeded in 6-well plates and transfected with 4 μg/well vectors expressing CasRx-GFP and gRNAs-mCherry (CasRx:gRNA-1:gRNA-2 = 2:1:1, see Supplementary sequences) using Lipofectamine 3000 reagent (Thermo Fisher Scientific). Control group was only transfected with 2 μg/well vectors containing CasRx-GFP ...
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bioRxiv - Neuroscience 2019Quote: ... Abcam ab13970, γH2A.X 1:500, Sigma Aldrich 05-636; Map-2 1:250, Sigma Aldrich M4403; Map-2 1:250, Invitrogen PA517646 ...
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bioRxiv - Cell Biology 2020Quote: ... cells (either MDA MB 231 GFP or a mixture of MDA MB 231 GFP and MCF 7 at a ratio of 1:1) were suspended in cold neutralized bovine collagen solution (2.5 mg/ml) (Thermo Fisher Scientific). This cell suspension was then added to pre-warmed (37 ̊C ...
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bioRxiv - Cell Biology 2020Quote: ... On day 7 the media was switched to repro media2 and E7 reprogramming media (1: 1) containing E6 basal media (Thermo Fisher) with NaB ...
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bioRxiv - Molecular Biology 2021Quote: ... mouse anti-ELAV (MAb 7D, 1:20, which recognizes 7 amino acids unique to ELAV) and anti-GFP (1:250; Invitrogen A11122) and visualized with Alexa Fluor 488- and/or Alexa Fluor 647-coupled secondary antibodies (1:250 ...