Labshake search
Citations for Thermo Fisher :
1951 - 2000 of 10000+ citations for TIM 1 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... HEK-293T cells were transfected with the FRG construct using Lipofectamine™ 3000 (Invitrogen, # L3000015). Supernatant was collected for 7 days and the antibody was purified using a protein A column (ThermoFisher scientific ...
-
bioRxiv - Neuroscience 2020Quote: ... RRID:CVCL_0063) and were maintained in standard HEK Media: DMEM (DMEM High glucose, pyruvate; Gibco 11995081) + 10% FBS (Qualified US Origin ...
-
bioRxiv - Genomics 2021Quote: ... Detachment of HEK cells for passaging was performed using the TrypLE reagent (Thermo Fisher, #12605010). All cell lines were cultured at 37°C ...
-
bioRxiv - Genomics 2022Quote: ... HEK- 293T cells were maintained in high glucose DMEM medium 1X with L-Glutamine (Invitrogen), supplemented with 100 U/mL penicillin and 100 mg/mL streptomycin (Gibco ...
-
bioRxiv - Immunology 2022Quote: 293TN HEK cells for lentiviral production were cultured in DMEM medium with 10% FCS (Gibco) and 1 mM sodium pyruvate ...
-
bioRxiv - Microbiology 2022Quote: ... Transfection of HEK-293T cells was carried out using Lipofectamine 2000 (Thermo Fisher Scientific - Invitrogen). Transfection conditions used a ratio of 1 µg total DNA (including all three plasmids ...
-
bioRxiv - Microbiology 2022Quote: ... Transfection of HEK-293T cells was carried out using Lipofectamine 2000 (Thermo Fisher Scientific - Invitrogen). Transfection conditions used a ratio of 1 µg total DNA (including all three plasmids ...
-
bioRxiv - Biophysics 2022Quote: HEK 293T/17 cells (ATCC) were maintained in high glucose Dulbecco’s Minimum Essential Media (Gibco) supplemented with 10% fetal bovine serum (Atlanta Biologicals) ...
-
bioRxiv - Microbiology 2023Quote: HEK 293T and Cos7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (ThermoFisher 11965118) supplemented with 10% heat inactivated fetal bovine serum (FBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... pLP2 and pLP/VSV-G into HEK 293T cells using TurboFect (#R0531, Thermo Fisher Scientific). Lenti-virions were collected after 3 days and concentrated by ultracentrifugation at 4 °C ...
-
bioRxiv - Immunology 2023Quote: ... Human embryonic kidney (HEK) 293T (ATCC® CRL-3216) cells were cultured in DMEM (Gibco) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Bioengineering 2022Quote: Lenti-X HEK 293T cells were transfected using Lipofectamine™ 3000 transfection reagent (ThermoFisher Scientific) following the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2022Quote: ... human HEK 293T cells were cultured at 37 °C in 5% CO2 in DMEM (ThermoFisher), with 10% FCS and 1% P/S ...
-
bioRxiv - Cell Biology 2023Quote: ... Culturing media for HEK-Orai1YFP cells was additionally supplemented with 4 mg/ml G418 (ThermoFisher). For biotinylation experiments ...
-
bioRxiv - Immunology 2024Quote: ... and HEK-293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Life Technologies, USA), while AsPC-1 ...
-
bioRxiv - Neuroscience 2024Quote: HEK-293T cells were transfected after reaching 70% confluency via Lipofectamine 3000 (Thermo Fisher Scientific) (2.5 μg total cDNA ...
-
bioRxiv - Neuroscience 2024Quote: Human Embryonic Kidney 293T (HEK) cells (ATCC) were maintained in high glucose DMEM media (Gibco). Media was supplemented with 10% FBS (Millipore Sigma ...
-
bioRxiv - Molecular Biology 2024Quote: ... 20,000 HEK-293T cells were seeded in each well of a LabTek chamberslide (#055071, Nunc). Cells were co-transfected using FuGENE 6 with 100 ng of pCMV6-NR2F2-WT or p.Arg246His and 100 ng of pCMX-NR5A1 ...
-
bioRxiv - Neuroscience 2024Quote: HEK-293T and HeLa cells were maintained in Dulbecco’s modified Eagle medium (DMEM) (Gibco, 1056910) supplemented with 10% heat inactivated Fetal Bovine Serum (FBS ...
-
bioRxiv - Microbiology 2024Quote: Expression of the full-length LUJV GPC was carried out in HEK 293F cells (Invitrogen) using FreeStyle Medium (Life Technologies) ...
-
bioRxiv - Developmental Biology 2021Quote: Coding sequence for sbp2 was PCR amplified from 0-5 dpf pooled cDNA using the forward and reverse primers shown in Table 1 and then TA cloned into pcDNA3.1 V5/His Topo (Thermo Fisher Scientific). For secisbp2l ...
-
bioRxiv - Neuroscience 2021Quote: His-tagged human dynamin 1 was expressed using the Bac-to-Bac baculovirus expression system (Thermo Fisher Scientific, Waltham, MA, USA) and purified as described previously (66) ...
-
bioRxiv - Cell Biology 2022Quote: ... recombinant baculovirus containing the sequence of ΔPRD-dynamin 1 with an N-terminal His-tag was generated by following Bac-to-Bac Baculovirus Expression System (ThermoFisher Scientific). The suspension cultures of Sf9 were maintained in Sf-900 III serum-free media (SFM ...
-
bioRxiv - Microbiology 2021Quote: ... recombinant SARS-CoV-2 Spike RBD protein (RBD, His Tag) was conjugated with EZ-LinkTM Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
-
bioRxiv - Pathology 2021Quote: ... His-tag recombinant SARS-CoV-2 Spike Protein S1/S2 (S-ECD) (1 ng/ml; ThermoFisher Scientific; aa11-1208; RP-87680) or Flag-tag recombinant SARS-CoV-2 Spike RBD (1 ng/ml ...
-
bioRxiv - Microbiology 2020Quote: ... The plasmids were transiently transfected into Expi293F cells using ExpiFectamine™ 293 Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2020Quote: 293 cells or HeLa cells were cultured in Dulbecco’s-modified Eagle’s medium (Invitrogen, cat. no. C11995500BT) added with 10% fetal bovine serum (FBS ...
-
bioRxiv - Immunology 2022Quote: FreeStyle 293-F (cat# R79007) and Expi293F cells (cat# A14528; RRID: CVCL_D615) were purchased from ThermoFisher Scientific Inc ...
-
bioRxiv - Molecular Biology 2021Quote: ... and recombinant expression was undertaken using the FreeStyle™ MAX 293 Expression System (Thermo Fisher Scientific) according to the manufacturer’s guidelines ...
-
bioRxiv - Bioengineering 2019Quote: ... Cells were washed with a FACS buffer containing FreeStyle™ 293 Expression Medium (Thermo Fisher Scientific) with 2% BSA and incubated with 15 µg/mL primary antibody ...
-
bioRxiv - Immunology 2021Quote: ... and were expressed in Expi 293F mammalian cells using Expifectamine 293 transfection reagent (Thermo Fisher Scientific) cultured for 4-5 days ...
-
bioRxiv - Cell Biology 2021Quote: ... Stable transfected HEK293T cells were generated with the Flp-In T-REx 293 cell system (Invitrogen) following the manual ...
-
bioRxiv - Biochemistry 2020Quote: Human embryonic kidney cells 293 (HEK293) cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Microbiology 2020Quote: ... and human embryonic kidney 293 cells (HEK293T) were maintained in Dulbecco’s modified Eagle medium (DMEM, Invitrogen) supplemented to contain 10% heat-inactivated FBS and 1% L-glutamine ...
-
bioRxiv - Microbiology 2020Quote: ... VLP-WT and VLP-cvD were expressed using ExpiFectamine™ 293 Transfection Kit (Thermo Fisher Scientific) following manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: Expi293F cells were transfected with the construct of interest using the ExpiFectamine 293 Transfection Kit (Gibco) following the manufacturer’s protocol for a 50 mL culture ...
-
bioRxiv - Microbiology 2022Quote: ... then re-suspended in an appropriate volume of FreeStyle 293 Expression Medium (no. 12338018; Thermo Fisher) to obtain a concentration of 1e8 cells/ml ...
-
bioRxiv - Microbiology 2021Quote: ... The plasmids were transiently transfected into Expi293F cells using ExpiFectamine™ 293 Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... Human embryonic kidney (293) cells were cultured in Dulbecco’s modified Eagle medium (DMEM with GlutaMax, Invitrogen), supplemented with 10% v/v foetal bovine serum ...
-
bioRxiv - Molecular Biology 2020Quote: ... HEK293 cells (Flp-In 293 T-REx cell lines) were obtained from Life Technologies (R780-07) (Invitrogen ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... generated from parental Flp-In™ T-REx™ 293 cells (Thermo Fisher Scientific, MA, USA) by Flp-mediated genomic integration of an insert encoding human GLP-1R with an N-terminal SNAPf-tag and C-terminal SmBiT tag (custom lingen ...
-
bioRxiv - Molecular Biology 2021Quote: ... Human embryonic kidney 293 cells containing SV40 large T-antigen (HEK293T) were cultured in DMEM (Gibco) supplemented with 10% (v/v ...
-
bioRxiv - Microbiology 2020Quote: ... The plasmids were transiently transfected into Expi293F cells using ExpiFectamine™ 293 Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... The plasmid was transiently transfected into Expi293F cells using ExpiFectamine™ 293 Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... trypsinized the cells were transferred to 250 mL flask in 100 mL 293 Freestyle medium (Gibco) supplemented with G418 and cultured in shaking incubator at 37°C with 5% CO2 ...
-
bioRxiv - Immunology 2021Quote: ... 80 ml HyClone SFM4HEK293 medium (Cytiva) and 80 ml FreeStyle 293 Expression Medium (Thermo Fisher Scientific) were added to each 0.8 liter of cells ...
-
bioRxiv - Immunology 2020Quote: ... 2.5×106 cells/ml were transfected using ExpiFectamine™ 293 transfection reagent (ThermoFisher, cat. no. A14524) as per manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2021Quote: HEK293 cells (Flp-In 293 T-REx cell lines) were obtained from Life Technologies (R780-07) (Invitrogen) ...
-
bioRxiv - Immunology 2021Quote: ... Plasmids encoding Spike-2P and HexaPro 61 were transiently transfected in FreeStyle 293 cells (Thermo Fisher) using Turbo293 (SpeedBiosystems ...
-
bioRxiv - Immunology 2020Quote: ... and grown at 37°C in Freestyle™ 293 Expression Medium (Thermo Fisher Scientific, catalog #12338002) in baffle-bottomed flasks orbiting at 135 rpm ...