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Citations for Thermo Fisher :
1951 - 2000 of 10000+ citations for SARS CoV 2 Spike N Terminal Domain NTD Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Samples were incubated with anti-sheep AlexaFluor 488 IgG (1:500, Invitrogen) in blocking buffer for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... goat anti-rabbit or donkey anti-sheep (all from Invitrogen, 1:1000). Coverslips were washed twice in wash buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... was coupled to 50 μl of Dynabeads Sheep-anti-Mouse IgG (Invitrogen) per sample ...
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bioRxiv - Cell Biology 2022Quote: ... AlexaFluor 594 donkey anti-sheep IgG (H+L) (Invitrogen, A11016, Lot: 536045); GFP-Booster ATTO 488 (Chromotek ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-mouse and anti-sheep Alexa Fluor 647-conjugated antibodies (Thermo Fisher), and anti-goat CF405M (Biotium).
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bioRxiv - Genomics 2022Quote: ... and using Dynabeads™ M-280 Sheep Anti-Rabbit IgG (Invitrogen 11203D). H3K27ac and H3K27me3 ChIP–seq libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645L ...
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bioRxiv - Neuroscience 2022Quote: ... or donkey anti-sheep 647 (cat. # A21448; Thermo Fisher Scientific; 1:500)) in diluting buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... or in THY agar medium supplemented with 5% sheep blood (Thermo Scientific) at 37°C in a 5% CO2 atmosphere ...
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bioRxiv - Neuroscience 2022Quote: ... and Alexa Fluor 647-conjugated donkey anti-sheep (1:1000, A21448, Invitrogen) and nuclear dye ...
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bioRxiv - Microbiology 2023Quote: ... with addition of 5% (v/v) defibrinated sheep blood (Thermo Scientific®). Growth was monitored by measuring the optical density at 600 nm (OD600).
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bioRxiv - Developmental Biology 2023Quote: ... and 10μL Dynabeads coupled with M-280 sheep anti-mouse antibody (Invitrogen). Pulled down DNA was amplified for 12 cycles with adapter-specific indexed primers ...
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bioRxiv - Cell Biology 2023Quote: IgG magnetic beads (Dynabeads M-280 Sheep α-Rabbit IgG, Life Technologies) were washed twice with IgG binding buffer (1X PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies: sheep anti-mouse HRP-conjugated (1:10,000, Thermo Fisher Scientific), donkey anti-rabbit HRP-conjugated (1:10,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-LRIG1 sheep polyclonal unconjugated antibody at 2.5 ug (Invitrogen #PA5-47928), and anti-LGR5 mouse monoclonal conjugated with PE at 1:100 (Origene #TA400001) ...
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bioRxiv - Pathology 2024Quote: ... labeled with the appropriate Alexa Fluor donkey anti-sheep 647 (Invitrogen, A21448) and anti-rabbit 488 (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Each virus was mixed with PBS-washed defibrinated sheep blood (Fisher Scientific) containing 5 mM ATP to a concentration of 106 PFU/mL and fed to ∼50-60 female mosquitoes ...
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bioRxiv - Neuroscience 2023Quote: ... or (donkey anti-sheep AF350, 1:200, Cat#A21097, Thermo Fisher Scientific) for 3 hrs at room temperature ...
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bioRxiv - Genetics 2023Quote: ... for H3K9me2 or Dynabeads M-280 Sheep anti-Mouse IgG (Thermo Fisher) for other antibodies for 2h at 4°C with rotation ...
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bioRxiv - Neuroscience 2024Quote: ... or donkey anti-sheep Alexa Fluor 488 (1:1000; Invitrogen, Waltham, USA) were used as secondary antibodies ...
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bioRxiv - Immunology 2024Quote: ... 200 µL of Dynabeads M-280 Sheep Anti-Mouse IgG (Invitrogen, #11201D) washed with wash buffer (5 mg/mL BSA in PBS ...
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bioRxiv - Immunology 2020Quote: ... and purified using Nickel-NTA resin for gB Domain II (Thermo Fisher Scientific), and lectin resin (VWR ...
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bioRxiv - Cell Biology 2020Quote: ... anti- HA-tag for KRAB protein (1:500, 32-6700 NOVEX HA epitope Tag antibody 5B1D10, Invitrogen, USA) and anti-b-Catenin (1:400 ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were next treated with 1: 1000 dilution of primary antibody (6-His tag mouse anti-tag, Invitrogen) for 1 hr at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... Human OTUD4 constructs were cloned without tag or with FLAG-tag into the expression plasmid pcDNA3.1 (Life technologies) using the oligos forward (BamHI ...
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bioRxiv - Cell Biology 2024Quote: ... Myc-tag immunoprecipitation (IP) was carried out using the Myc-tag magnetic IP kit (Thermo Fisher Scientific, 88844), following the manufacturer’s recommended protocol.
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bioRxiv - Biochemistry 2022Quote: 250 μg of protein from HEK-293T cell lysates were incubated overnight with 2 μg of a 6x-His tag antibody (MA 1-135, Thermo Fisher) in the presence of a low–salt RIPA buffer (20mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... Immunoprecipitation of TgTRPPL-2-smHA protein was performed using the Pierce HA Tag/Co-IP Kit (Thermo Fisher Scientific, Waltham, MA) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: The primary antibodies were as follows: mouse anti-HA tag (2-2.2.14) antibody (1:1000; Thermo Fisher Scientific, cat. no. 26183); rabbit anti-HA tag (C29F4 ...
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bioRxiv - Genetics 2021Quote: ... The lysate was cleared by centrifugation and incubated for 2 hours at 4°C with Tag specific magnetic beads (Dynabeads™ M-280 Streptavidin/Invitrogen for immunoprecipitation of Avitag-ZFP462 and Avitag-GLP ...
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bioRxiv - Molecular Biology 2021Quote: ... Gene fragments encoding multiple (2 to 9) HA-tags were designed in silico and ordered using GeneArt Gene Synthesis service from Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide HCl (50-848-678, Fisher Scientific), butyric acid (B103500-100ML ...
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bioRxiv - Physiology 2020Quote: ... 0.05% 3-(N,N-Dimethylmyristylammonio) propanesulfonate zwittergent detergent (Acros Organics, 427740050)) containing protease inhibitor (Mammalian ProteaseArrest-APExBIO K1008) ...
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bioRxiv - Developmental Biology 2022Quote: ... 80□μL of N-methyl-N-(trimethylsilyl) trifluoroacetamide□(Thermo Fisher Scientific) and 70□μL ethyl acetate (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... and rinsed with N,N-Dimethylformamide (DMF) (Thermo Fisher certified ACS) for several min while upside down with agitation until there were no visible air bubbles ...
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bioRxiv - Immunology 2019Quote: ... whole stomachs were finely minced in disassociation buffer (2% FCS in Hank’s Balanced Salt Solution without calcium and magnesium (Life Technologies) and 5 mM EDTA ...
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bioRxiv - Immunology 2021Quote: Released tissue cells were stained in aliquots of 1 ×106 cells/100 μL of PBS with 2% fetal calf serum (FCS, GIBCO) and 0.1% NaN3 for 30min on ice ...
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bioRxiv - Immunology 2021Quote: ... NK cells were then expanded for 6 days with hIL-15 50ng/ml (Miltenyi #130-095-765) in RPMI 20% FCS supplemented with L-glutamine (2 mM; Gibco), penicillin/streptomycin (100 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... F-actin staining was performed at 4°C overnight in PBS 1x containing 0,1% TritonX100 2% FCS with Phalloidin AF405plus (Life technologies). Samples were washed in PBS 1x and mounted with Prolong Antifade Gold mounting medium (Life technologies) ...
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bioRxiv - Developmental Biology 2021Quote: ... 4° C following by re-suspending the cells in 0.5-1 of ml cell isolation media (2% FCS, DPBS:HBSS=1:1 and 1mM SYTOX Blue Dead Cell stain (ThermoFisher Scientific). Cells were imaged before FACS and after FACS to confirm successful purification of GFP+ cells.
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bioRxiv - Immunology 2020Quote: ... gradient centrifugation and resuspended at 1-2 x 106 cells/ml in RPMI-10% FCS (RPMI 1640 w/L-glutamine (Cellgro Gibco) supplemented with 10% FCS (Cellgro Gibco) ...
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bioRxiv - Immunology 2021Quote: ... mice were directly perfused with PBS/ 2 % FCS/ 1 mM EDTA and liver digest medium (ThermoFisher Scientific/ Gibco, Waltham, Massachusetts) through the vena cava ...
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bioRxiv - Microbiology 2021Quote: ... The plate was incubated for approximately 5 min before the reaction was stopped with the addition of 2 M H2SO4 and the A450 was measured (Multiskan FC, ThermoFisher). A standard curve was established using serial dilutions of lysate from PA11 ...
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bioRxiv - Immunology 2022Quote: ... 2020) were cultured in R10 medium (RPMI1640 [Gibco Life Technologies, 31870-025], 10% FCS, 2 mM glutamate [Gibco Life Technologies, 25030-024] ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Content of two ampules containing the cell culture was resuspended in Hank’s solution supplemented with 2% FCS (fetal calf serum) (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... After two washes of the tissue with plating medium (DMEM containing 10% FCS, 2 mM glutamine, 50 mg/ml penicillin/streptomycin, Invitrogen), it was triturated in plating medium and subsequently cells counted ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with plasmids expressing bRSV N-GFP and P in DMEM supplemented with 2% FCS.16 h post transfection medium was replaced with Leibovitz’s L-15 medium without phenol red (Gibco, ThermoFisher Scientific), and imaged live with a Leica TCS SP8 confocal microscope using a 63× oil immersion objective ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were washed with PBS and resuspended in 200 µl buffer (PBS supplemented with 2% FCS) and analyzed via Attune NxT Flow Cytometer (ThermoFisher). For LIVE/DEAD analysis all cells were gated and viable cells were quantified as indicated in Figure 4 via FlowJo (Version 10.9.0 ...
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bioRxiv - Bioengineering 2023Quote: ... cells in each well were firstly washed with flow cytometry staining buffer: 3% FCS with 2 mM EDTA (15575020; Invitrogen) in PBS− ...
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bioRxiv - Genetics 2023Quote: ... resuspended in 800 μL of PBS supplemented with 2 % FCS and analyzed on Attune NxT Flow Cytometer (Thermo Fisher Scientific).
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bioRxiv - Immunology 2024Quote: ... Enzymes were quenched with PBS with 2mM EDTA and 2% FCS on ice and then red blood cells were lysed with room-temperature ACK lysis buffer (Gibco) and passed through a 100μm cell strainer.