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Citations for Thermo Fisher :
151 - 200 of 10000+ citations for Recombinant Mouse Scavenger Receptor Class B Member 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... PCR amplicons were digested and ligated into pcDNA3.1 myc-His B expression vectors (Invitrogen), with a 3xFlag epitope tag also fused to the N-terminus of Ptchd1 ...
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bioRxiv - Biochemistry 2022Quote: ... recombinant human RPA or RPA-variants (2 μM) were incubated with 250 nM of recombinant Aurora B kinase (Invitrogen Inc.) in kinase reaction buffer (50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... The monoclonal mouse anti-ryanodine receptor (RyR, MA3-916, Thermo Fisher) primary antibody was incubated overnight (4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-Transferrin receptor mAb (clone H68.4, Invitrogen, no. 13-6800,), rat anti-HA-tag mAb (clone 3F10 ...
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bioRxiv - Immunology 2023Quote: ... Fc receptors were blocked by incubating cells with 1:50 anti- mouse CD16/CD32 (Fc block, Invitrogen) for 10 minutes at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... sections were rinsed in 0.1 M PBS and incubated in Alexa Fluor 647-tagged goat anti-mouse IgG or Alexa Fluor 750-tagged goat anti-rabbit IgG (1:100, Thermo Fisher, cat# A21235 and A21039), or Alexa Fluor 647-tagged donkey anti-mouse IgG ...
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bioRxiv - Cell Biology 2024Quote: ... Mouse IgG1 anti-alpha Tubulin Clone B-5-1-2 (1:500, ThermoFisher 32-2500), Rabbit anti-Myc polyclonal (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... After blocking supernatant samples from different groups and standards (recombinant human-TREM2-His; Life Technologies) were incubated for 2 h at room temperature (RT ...
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bioRxiv - Pathology 2021Quote: ... His-tag recombinant SARS-CoV-2 Spike Protein S1/S2 (S-ECD) (1 ng/ml; ThermoFisher Scientific; aa11-1208; RP-87680) or Flag-tag recombinant SARS-CoV-2 Spike RBD (1 ng/ml ...
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bioRxiv - Neuroscience 2020Quote: ... fluorescent conjugate cholera toxin B (CTB; recombinant cholera toxin subunit B conjugated with Alexa fluorophores: 0.2% CTB-488 and CTB-594; Life Technologies) was injected into AL and PM.
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bioRxiv - Cell Biology 2020Quote: Parent CHO-K1 and CHO-K1 cells stably expressing extracellular ACP-tagged human insulin receptors were maintained at 37°C with 5% CO2 in HAM’s F-12 medium (Thermo Fisher) supplemented 10% fetal bovine serum (FBS) ...
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bioRxiv - Molecular Biology 2020Quote: ... the receptor Fc-tagged ectodomains present in the conditioned media were captured on protein A-coated 384-plates (Thermo Scientific), and stored at 4 °C until use ...
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bioRxiv - Biochemistry 2023Quote: ... 155-238) were fused to the C-termini of tagged receptors and cloned into the NheI-XhoI sites of pCEP4 (Invitrogen). Plasmids for HIV-1 MN gp160 and BaL gp160 (# 100919 ...
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bioRxiv - Biochemistry 2020Quote: ... Mouse anti-Penta-His-tag (#P-21315, ThermoFisher Scientific), 1:2000 ...
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bioRxiv - Microbiology 2022Quote: ... or the mouse-anti-His (HIS.H8) primary antibody (Invitrogen) (1:1,000 ...
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bioRxiv - Immunology 2022Quote: ... mouse anti-His antibody (Invitrogen cat. # MA1-21315-1MG) was used at the same concentration to coat the plates ...
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bioRxiv - Microbiology 2023Quote: ... Lanes reacted with anti-His antibody were incubated for 1.5 hours in mouse anti 6X His antibody (Invitrogen MA1-21315, 1:500 in TBST) and lanes reacted with S-tag antibody were incubated in rabbit anti-mouse S-tag antibody (Invitrogen PIPA 581631 ...
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bioRxiv - Biochemistry 2020Quote: Both untagged and His-tagged EncFtn proteins were quantified using the Pierce BCA Assay kit (Thermo Fisher Scientific) following the manufacturer’s instructions for the Test-tube procedure ...
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bioRxiv - Cell Biology 2024Quote: ... His-tagged: The lysate was mixed head-over-head with Ni-NTA agarose resin (Thermo Scientific™, USA) for 60 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: LZTR1WT and LZTR1L580P were expressed as C-terminal His-tagged proteins in Expi-293F cells (Thermo Fisher Scientific). pcDNA3.1-LZTR1-Myc-6xHis plasmid was a gift from Jens Kroll (Heidelberg University and German Cancer Research Center (DKFZ-ZMBH Alliance)).56 The LZTR1L580Pvariant was introduced into the plasmid by site-directed mutagenesis as previously described.11 Cells were transfected using ExpiFectamine 293 Reagent (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Recombinant SARS-CoV-2 receptor binding domain was coated onto MaxiSorp 96-well plates (Thermo Scientific, Cat # 442404) at 200 ng/well at 4 °C overnight ...
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bioRxiv - Bioengineering 2023Quote: ... at a concentration of 2μg per sample and anti-mouse MHC class II antibody conjugated to FITC (Clone: M5/114.15.2) (Invitrogen, USA) at a concentration of 5μg per sample ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1000 U/mL recombinant mouse LIF (Invitrogen).
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bioRxiv - Microbiology 2022Quote: ... monoclonal transferrin receptor antibody (H68.4, Invitrogen, 1:1000) and monoclonal StrepII (C23.21 ...
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bioRxiv - Cell Biology 2023Quote: ... anti-Transferrin Receptor (1:1000, 13-6800, Invitrogen), anti-LC3B (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... anti-Transferrin Receptor (1:100, 13-6800, Invitrogen), anti-LC3B (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... and transferrin receptor (ThermoFisher 13-6800, 1:1,000) antibodies.
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bioRxiv - Cell Biology 2021Quote: ... Recombinant cells were selected using 150 μg/ml of Hygromycin B (Invitrogen, Paisley, U.K.).
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bioRxiv - Neuroscience 2022Quote: ... An injection of Cholera Toxin Subunit B (recombinant) conjugated to Alexa Fluor 647 (ThermoFisher) was made in the striatum of the same hemisphere (+1.2mm AP ...
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bioRxiv - Neuroscience 2023Quote: ... Fetal brains were evaluated by Western blot (corticotropin releasing factor receptor 1 [CRFR1]; glucocorticoid receptor [GR], interleukin [IL]-6 receptor [IL-6R]; IL-17A and β-actin, Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Microbiology 2022Quote: ... for 1 hour at room temperature followed by additional 1 hour incubation with 1:200 dilution of fluorescence-tagged goat anti-mouse IgG Alexa Fluor 488 (Thermofisher) (Supplemental Table 5) ...
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bioRxiv - Biochemistry 2020Quote: ... His-tagged Gβγ was expressed in Sf9 cells for 2 days following baculovirus infection (pFastBacDual, Bac-to-Bac, Invitrogen). Cells were harvested by centrifugation and lysed by sonication in 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: His-tagged P-Rex2 was expressed in Sf9 cells for 2 days following baculovirus infection (Bac-to-Bac, Invitrogen). Cells were harvested by centrifugation and lysed by sonication in 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: Detergent extracted His-MBP-tagged proteins were applied to a 40 mL POROS MC 20 Ni2+ column (Applied Biosystems) equilibrated with buffer A (200 mM NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... SmBChE1 and SmAChE3 (fSmChEs) were EcoRI/XbaI cloned into the C-terminal 6-His-tagged pPICZαA expression vector (Invitrogen) to facilitate secretory expression ...
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bioRxiv - Biophysics 2023Quote: ... and the 3C protease was removed using Dynabeads magnetic beads designed for His-tagged protein isolation (Thermo Fisher, 10103D). The supernatant from the magnetic bead pulldown was then concentrated using Amicon 10 kDa centrifugal filter units (MilliporeSigma) ...
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bioRxiv - Cell Biology 2023Quote: ... His-tagged TpPyShell together with TEV protease was dialyzed overnight at 4°C in SnakeSkin dialysis tube (Thermo Scientific) against dialysis buffer (50 mM Tris-HCl pH8.0 ...
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bioRxiv - Physiology 2020Quote: ... βTC3 cells were transfected with plasmids encoding mouse furin and/or mouse insulin receptor (pcDNA3.1 backbone) and/or mouse Atp6ap1/Ac45-Flag using Lipofectamine 2000 (Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Transferrin (loading control) was detected with mouse anti-human transferrin receptor antibody (1:500, Cat# 136800, Invitrogen, Waltham, MA). Protein samples (50 µg ...
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bioRxiv - Molecular Biology 2023Quote: Plasmid CassetteAv2_pBAD contains the CRISPR03 array cloned into the pBAD/Myc–His B backbone (Life Technologies).12 It was used to prepare CRISPR DNA substrates by PCR with primers MMB1Lead40-5 and MMB1crisp3-r1 using Phusion High-Fidelity DNA polymerase according to the manufacturer’s protocol (New England Biolabs or ThermoFisher) ...
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bioRxiv - Cell Biology 2019Quote: ... 1 × B-27 (Gibco), 1.25 mM N-acetylcysteine (Sigma) ...
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bioRxiv - Cancer Biology 2022Quote: ... 1× B-27 (GIBCO), 1× N2 (GIBCO) ...
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bioRxiv - Microbiology 2019Quote: ... 1 % amphotericin B (Gibco), and 20 ng/mL macrophage colony-stimulating factor (mCSF ...
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bioRxiv - Microbiology 2020Quote: ... 1% amphotericin B (Gibco)] ...
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bioRxiv - Microbiology 2023Quote: ... 1× B-27 (Gibco), 1× N-2 (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1% B-27 (Gibco), 1% GlutaMAX (Gibco) ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-NMDA receptor subunit 1 (1:1000, ThermoFisher PA3-102), and chicken anti-tyrosine hydroxylase (1:500 ...
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bioRxiv - Biochemistry 2020Quote: ... The expression of the recombinant protein was measured by ELISA using anti‐His tag monoclonal antibody (Invitrogen) to capture and biotinylated anti‐ANG1 antibody for detection (R&D System) ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant AtCSD1 protein containing a 6xHis-tag was purified using Dynabeads His-Tag (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: Recombinant Rep1ΔAD-His and its variants were incubated with 50 μl D–Galactose agarose beads (Thermo Scientific) overnight at 4 °C ...