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Citations for Thermo Fisher :
151 - 200 of 10000+ citations for Mayaro Virus E2 Envelope Protein Mouse Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... anti-HA epitope tag (#901513) and Alexa Fluor 647 anti-mouse IgG (Invitrogen #A21235).
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bioRxiv - Systems Biology 2020Quote: ... and 0.6μg pMD2.G (encoding VSV G envelope protein) was diluted in 2000μl Opti-MEM® Reduced Serum medium (Gibco™/ Life Technologies). 5.4μl PLUS™ Reagent was added directly to the diluted DNA and incubated for 5min at RT ...
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bioRxiv - Systems Biology 2020Quote: ... and 0.6μg pMD2.G (encoding VSV G envelope protein) was diluted in 2000μl Opti-MEM® Reduced Serum medium (Gibco™/ Life Technologies). 5.4μl PLUS™ Reagent was added directly to the diluted DNA and incubated for 5min at RT ...
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bioRxiv - Microbiology 2019Quote: ... CR-Fc or DC-SIGN-Fc (Thermofisher) in binding buffer (BB ...
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bioRxiv - Molecular Biology 2020Quote: ... and 260 ng pMD.G envelope plasmid using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 0.25 µg envelope plasmid diluted in 100 µl optiMEM (Gibco) was mixed with 20 µl of PolyFect transfection reagent (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... Media containing virus particles was first concentrated to 20x using 100K MWCO protein concentrator columns (Thermofisher). Concentrated virus was then incubated using 1:2000 DiD-Cell labelling solution (ThermoFisher V22887 ...
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bioRxiv - Immunology 2023Quote: ... Clec12a-Fc or Fc only fusion proteins were isolated with Pierce™ Classic Magnetic IP/Co-IP Kit (Thermo Fisher Scientific, Catalog number: 88804), quantified with Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific Catalog number ...
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bioRxiv - Molecular Biology 2021Quote: ... Fab and Fc regions were separated using a Nab Protein A column (Thermo Scientific). Fabs were concentrated up to ~1 mg/mL using an Amicon Ultra 0.5 filter (10k cut-off ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant hACE2-Fc protein was first biotinylated using EZ-Link Sulfo-NHS-Biotin (ThermoFisher) as the instruction described ...
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bioRxiv - Synthetic Biology 2023Quote: ... and Fc fusions were purified with Protein A agarose resin (Thermo Fisher Scientific; 20334) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... Fc-PRL-13 was purified with Protein A agarose resin (Thermo Fisher Scientific; 20334) according to the manufacturer’s instructions with the exception that all buffers were supplemented with 1tab/50mL of cOmplete Protease Inhibitor Cocktail and 10uM Pepstatin A ...
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bioRxiv - Biochemistry 2020Quote: ... Cdc50 proteins fused to the 10xHis tag were detected using the HisProbeTM-HRP conjugate (ThermoFisher) diluted to 1:2000 in 2 % (w/v ...
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bioRxiv - Biochemistry 2019Quote: ... The GST tag of the VP8* protein was removed using the thrombin (Thermo Fisher Scientific) after dialysis into the buffer (20 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2019Quote: ... washed and resuspended in FC buffer with goat-anti-mouse Alexa488 (Invitrogen; 1:400) for an additional 15 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Detection was performed with secondary goat anti-mouse IgG Fc HRP antibody (Invitrogen, A16084) and WesternBright Sirius chemiluminescent HRP conjugate (advansta ...
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bioRxiv - Microbiology 2020Quote: ... as measured on protein level (BCA protein concentration determination kit; before digestion) was labeled with 10-plexing tandem mass tags (TMT-10; Thermo Scientific) following the manufactures instructions ...
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bioRxiv - Neuroscience 2019Quote: ... TAG concentrations were normalized to the total protein content as determined by a Micro BCA protein assay kit (ThermoFisher, Cat # 23235). Each assay was done in triplicate and the average TAG level (μg TAG/ μg protein ...
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bioRxiv - Biochemistry 2021Quote: ... supplemented with 10% FCS (FCS, Thermo Fisher Scientific), 100 U ml−1 penicillin ...
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bioRxiv - Microbiology 2019Quote: ... mouse anti-6x-His epitope tag monoclonal antibody (Thermo Fisher Scientific, Cat. No. MA1-21315); LI-COR IRDye 800CW goat anti-rabbit IgG (LI-COR ...
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bioRxiv - Immunology 2022Quote: ... mouse anti-GFP HRP conjugated GFP tag ( 1:1000, Cat #HRP-66002, ThermoFisher, Waltham, MA), mouse anti-Prom1 (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... V5 tag to detect CBUA0028 or the Xpress tag (Invitrogen) to detect CBU0665.
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bioRxiv - Microbiology 2022Quote: ... V5 tag to detect CBUA0028 or the Xpress tag (Invitrogen) to detect CBU0665.
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bioRxiv - Cell Biology 2021Quote: ... HA-tag (Invitrogen), Flag-tag (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... Fc-fusion proteins were expressed via transient transfection of suspension-cultured CHO-S (Invitrogen/ThermoFisher) cells using the Mirus Bio ...
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bioRxiv - Immunology 2020Quote: ... Fc-fusion proteins were expressed via transient transfection of suspension-cultured CHO-S (Invitrogen/ThermoFisher) cells using the Mirus Bio ...
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bioRxiv - Cell Biology 2022Quote: ... the recombinant ACE2-Fc protein which was obtained from ExpiCHO™ expression system (Thermo Fisher) was 5-fold serially diluted in DMEM medium supplementing 10% FBS (Gibco) ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant hACE2-Fc protein was biotinylated using the EZ-Link sulfo-nhs-biotin kit (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The Fc-tagged ACE2 or mAb were first immobilized on Protein G Dynabeads (ThermoFisher Scientific). The S protein solutions were then incubated with on-bead receptor or antibody at room temperature for 60 min ...
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bioRxiv - Microbiology 2024Quote: Intact VHH-Fc proteins were separated on an Ultimate 3000 HPLC system (Thermo Fisher Scientific) online connected to an LTQ Orbitrap Elite mass spectrometer (Thermo Fischer Scientific) ...
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bioRxiv - Immunology 2020Quote: ... or with two administrations two weeks apart of 2.5μg Spike protein (2019-nCoV Spike Protein-S1+S2 ECD, His tag, SinoBiological) formulated in final 20mg/ml Imject™ Alum Adjuvant (Thermo Scientific) via bilateral intramuscular injections in the quadriceps (50μl per side) ...
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bioRxiv - Microbiology 2021Quote: ... Conditional supernatants from lentivirus-infected CHO cells sorted by FACS were used to purify the FXa-Fc fusion protein using a protein G column (89927, Thermo Fisher). For in vivo testing ...
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bioRxiv - Systems Biology 2021Quote: ... The protein digests were labeled with 10-plex Tandem Mass Tag (TMT) reagents (Thermo Fisher Scientific) (Dayon et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... Then Flag tag was captured with Pierce Protein A/G Agarose (20 μL per sample, ThermoFisher) at room temperature for 2 h with mixing constantly ...
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bioRxiv - Microbiology 2022Quote: ... The protein with a C-terminal Strep-tag was expressed in Expi293 cells (Thermo Fisher Scientific) and subsequently purified using Strep-Tactin XT Superflow high capacity resin (IBA Lifesciences ...
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bioRxiv - Microbiology 2020Quote: ... Bound protein was detected via the C-terminal 6XHis Tag using 6XHis Antibody MA1-135 (Invitrogen) followed by three washes in TBS-T and incubation with donkey anti-mouse horseradish peroxidase (HRP)-coupled secondary antibody (Dianova) ...
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bioRxiv - Immunology 2024Quote: ... the lysate supernatant containing His-tagged proteins was affinity-purified with His-Tag magnetic beads (Invitrogen) and used for pull-down assays ...
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bioRxiv - Neuroscience 2024Quote: ... extracted protein was immunoprecipitated using 25μL prewashed HA Ab-Tag Magnetic beads (Thermo Fisher Scientific, Massachusetts) with gentle rotation at 4°C for 30min ...
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bioRxiv - Microbiology 2019Quote: ... GST-tag and 3xFLAG-tag fusions were achieved using pDEST27 (Invitrogen) and pCI-neo-3xFLAG vector ...
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bioRxiv - Cell Biology 2023Quote: ... BacMam 2.0 virus (Invitrogen, C10593).
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bioRxiv - Immunology 2020Quote: ... ECD region of mouse CLEC2 was cloned into mammalian expression vector pFuse-rIgG-Fc (Invitrogen). The construct was used to stable transfect HEK-293T cells with PEI method ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were blocked with anti-mouse CD16/32 (Fc-block, Invitrogen, San Jose, CA, USA) for 10 minutes at 4°C followed by staining with antibody cocktail containing Pacific-Blue-conjugated anti-mouse CD45 (clone 30-F11 ...
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bioRxiv - Microbiology 2021Quote: ... and an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Invitrogen, Cat. No. 31437). MLV P30 protein within the pseudovirus capsid was detected using a rabbit anti-MLV-P30 polyclonal antibody (Origene ...
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bioRxiv - Microbiology 2021Quote: ... and an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Invitrogen, Cat. No. 31437). Beta-actin was used as an internal control.
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bioRxiv - Immunology 2022Quote: ... Fc-receptors were blocked with anti-mouse CD16/CD32 (20 µg/ml, clone 93; Invitrogen) for 20min ...
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bioRxiv - Bioengineering 2023Quote: ... Goat anti-mouse IgG Fc secondary antibody (A16084) HRP (Horseradish peroxidase) was purchased from Invitrogen. Goat anti-mouse IgG1 and IgG2c Fc secondary antibodies (ab97250 ...
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bioRxiv - Bioengineering 2023Quote: ... Goat anti-mouse IgG Fc secondary antibody (A16084) HRP (Horseradish peroxidase) was purchased from Invitrogen. Goat anti-mouse IgG1 and IgG2c Fc secondary antibodies (ab97250 ...
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bioRxiv - Immunology 2023Quote: ... Goat anti-mouse IgG Fc secondary antibody (A16084) HRP (Horseradish peroxidase) was purchased from Invitrogen. Alexa Fluor 488 Anti-alpha 1 Sodium Potassium ATPase antibody (ab197496) ...
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bioRxiv - Neuroscience 2022Quote: Antibodies used for these experiments include: anti-HA-tag (Mouse, ProteinTech, Thermo Fisher, 50-173-6449), phospho-TAOK2 (S181 ...
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bioRxiv - Molecular Biology 2022Quote: ... The following antibodies were used for Cut&Run: mouse monoclonal anti-V5 tag (ThermoFisher, # R960-25) diluted to 0.01 mg/ml ...