Labshake search
Citations for Thermo Fisher :
151 - 200 of 8564 citations for IL 9 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2021Quote: ... Supernatant was harvested at the indicated timepoints and IL-2 levels in the supernatant were measured via IL-2 Human Instant ELISA kit (Thermo Fisher #BMS221INST). T-cell proliferation was also measured at the indicated timepoints using a BD FACSymphony Fortessa X-50.
-
bioRxiv - Biochemistry 2023Quote: ... Detection of bound Af2-IL was performed using a rat anti-human IL-2 mAb Alexa Fluor488 conjugate (Invitrogen, Carlsbad CA, USA). Cell nuclei were counterstained with DAPI (Sigma Aldrich ...
-
bioRxiv - Microbiology 2023Quote: ... The level of IL-8 in cell culture supernatants was determined using a Human IL-8 Uncoated ELISA kit (Invitrogen, ThermoFisher Scientific Inc.).
-
bioRxiv - Microbiology 2020Quote: ... TNFα using the ProcartaPlex high sensitivity 9-Plex Human Panel (EPXS090-12199-901, Thermofisher Scientific, Waltham, MA). Samples were measured using a Luminex MAGPIX instrument (Luminex Corporation ...
-
bioRxiv - Immunology 2022Quote: ... HEK293 cells stably transfected with human CXCR4 (HEK293- CXCR4) were used at passage 5 and were cultivated in DMEM medium (Gibco), supplemented with 10% FCS and 1% penicillin/streptomycin (Gibco) ...
-
bioRxiv - Molecular Biology 2020Quote: The template RNA for cDNA synthesis was isolated from human HEK293 cell culture with TRIzol reagent (Thermo Fisher Scientific, USA), or FFPE samples ...
-
bioRxiv - Molecular Biology 2023Quote: Human embryonic kidney (HEK293) cells (RIKEN Cell Bank, Japan) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, GIBCO, 11965-092) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Developmental Biology 2022Quote: Human HEK293 cells were maintained at 37°C in complete media consisting of DMEM with high glucose GlutaMAX Supplement (Gibco) and 10% Fetal Bovine Serum ...
-
bioRxiv - Bioengineering 2024Quote: Human Embryonic Kidney (HEK293; ATCC Ref: CRL-1573) cells were cultured in Dulbecco’s Modified Eagle Medium + GlutaMAX (Gibco; 10569-010) supplemented with 10% fetal bovine serum (Biowest ...
-
bioRxiv - Bioengineering 2024Quote: Human Embryonic Kidney (HEK293; ATCC Ref: CRL-1573) cells were cultured in Dulbecco’s Modified Eagle Medium + GlutaMAX (Gibco; 10569-010) supplemented with 10% fetal bovine serum (Biowest ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The ELISA was performed using Human IL-1ẞ high sensitivity kit sold by Invitrogen. The sample for our ELISA consisted of cell culture media (referred to as sample hereafter ...
-
bioRxiv - Immunology 2022Quote: ... Human and mouse IL-1β secretion was quantified by ELISA kits (Thermo Fisher Scientific) according to the manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2022Quote: ... ELISA was performed with Human IL-8 ELISA Ready-SET-Go kit (Affymetrix eBioscience) according to manufacturer’s protocol.
-
bioRxiv - Cell Biology 2022Quote: ... and IL-17A Human Uncoated ELISA Kit (all Thermo Fisher Scientific, Waltham, MA, USA), respectively ...
-
bioRxiv - Cell Biology 2020Quote: ... HEK293 stably expressing the tetracycline (tet)-repressor (Flp-In T-REx HEK293, Invitrogen, Carlsbad, CA, USA) were cultured as above with the addition of 50 μg/mL zeocin and 5 μg/mL blasticidin (Invitrogen ...
-
bioRxiv - Biochemistry 2019Quote: HEK293 cells: HEK293 cells (CLS Cat# 300192/p777_HEK293, RRID:CVCL_0045) were cultured in DMEM high glucose (Gibco) and supplemented with 10% FCS (Gibco) ...
-
bioRxiv - Microbiology 2023Quote: ... The level of IL-8 in cell culture supernatants was determined using a Human IL-8 Uncoated ELISA kit (Invitrogen, ThermoFisher Scientific Inc.).
-
bioRxiv - Zoology 2023Quote: ... Supernatants were collected 24 hours post-treatment and then IL-8 secretion was quantified using commercial human IL-8 ELISA kits (Invitrogen, cat. #88-8086) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2022Quote: ... and activated for 48h with 30 U/ml human IL-2 and anti-CD3-anti-CD28-beads (Dynabeads™ human T-Activator CD3/CD28, ThermoFisher). Retroviral packaging cells RD114 were seeded to reach a confluency of 60% on the day of transfection and subsequently transfected with plasmids containing the neoTCR-α- and -β-chain-sequences using TransIT®-293 (MirusBio) ...
-
bioRxiv - Biochemistry 2021Quote: ... FreeStyle HEK293 cells (Thermo fisher scientific) were transfected with pcDNA3.1 vector encoding for HSulf-2 cDNA flanked by TEV cleavable SNAP (20.5 kDa ...
-
bioRxiv - Cell Biology 2021Quote: Low-passage HEK293 cells (Thermo Fisher) were co-transfected with 10 μg lentiviral vector (pCDH-SYN1-sypHy ...
-
A nepenthesin insert allosterically controls catalysis in the malaria parasite protease plasmepsin VbioRxiv - Microbiology 2022Quote: ... we transfected HEK293-F cells (ThermoFisher) using a modified version of the protocol in (41) ...
-
bioRxiv - Biochemistry 2021Quote: HEK293 Flp-In T-Rex (Invitrogen), HeLa (Austin et al. ...
-
bioRxiv - Biophysics 2021Quote: Suspension-adapted HEK293 Freestyle cells (Invitrogen) in serum free media (Invitrogen ...
-
bioRxiv - Molecular Biology 2022Quote: HEK293 TREx cells (Invitrogen Canada Inc.) were cultured in high-glucose Dulbecco’s modified Eagle’s medium (DMEM ...
-
bioRxiv - Microbiology 2023Quote: ... the Expi-HEK293 cells (Thermo Scientific) were transfected with the mammalian expression plasmid pHLSec containing the recodonized SUB1 ...
-
bioRxiv - Microbiology 2023Quote: ... and HEK293-S cells (ThermoFisher Scientific) were cultured in FreeStyle293 expression medium (Life Technologies) ...
-
bioRxiv - Immunology 2023Quote: ... FreeStyle™ HEK293-F cells (Gibco) were co-transfected with two plasmids ...
-
bioRxiv - Plant Biology 2019Quote: ... 1 μL of each reaction was added to a mix containing 9 μL of Hi-Di Formamide (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) and 0.5 μL of GeneScan 500 LIZ Size Standard (Applied Biosystems ...
-
bioRxiv - Plant Biology 2019Quote: ... 1 μL of each reaction was added to a mix containing 9 μL of Hi-Di Formamide (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) and 0.5 μL of GeneScan 500 LIZ Size Standard (Applied Biosystems ...
-
bioRxiv - Biochemistry 2020Quote: ... 6x-His-tagged constructs of human and murine SNED1 cloned into pCDNA5/FRT (Thermo Fisher, Waltham, MA) between the FseI and AscI sites were used to transiently transfect 293T cells to validate the anti-SNED1 antibody generated in this study (Supplementary Figure S1) ...
-
bioRxiv - Immunology 2022Quote: Human bladder carcinoma 5637 cells (ATCC, HTB-9) were grown in RPMI-1640 media (GIBCO, cat.no. 21875-034) containing 10% fetal bovine serum (FBS ...
-
bioRxiv - Immunology 2023Quote: ... Expression constructs for M35-V5/His and M27-V5/His (both in pcDNA3.1-V5/His, Invitrogen) have been described previously (Munks et al ...
-
bioRxiv - Biophysics 2019Quote: Human embryonic kidney cells 293 (HEK293-6E, NRC, Canada) were cultured in FreeStyle F17 expression medium (Thermo Fisher Scientific, Waltham, MA) supplemented with 2 mM L-glutamine (Invitrogen ...
-
bioRxiv - Cell Biology 2021Quote: ... N-terminal FLAG-tagged human MCC (P23508-1) constructs (pCMV2B vector) were transfected into HEK293 cells and selected with G418 (Gibco #10131035). G418-resistant cells were grown ...
-
bioRxiv - Biophysics 2021Quote: Human wild type ABCG2 or ABCG2 R184A containing an N-terminal Flag-tag was expressed in HEK293-EBNA (Thermo Fisher Scientific) cells as previously described19 ...
-
bioRxiv - Molecular Biology 2021Quote: ... the coding sequence encoding human TSPO (hTSPO) was amplified from cDNA previously generated by reverse transcription from total HEK293 mRNA isolated using Trizol (Life Technologies) and cloned into either a pENTR/SD/TOPO vector (Invitrogen ...
-
bioRxiv - Genetics 2024Quote: Flag-tagged wild type and mutated human RBBP5 plasmid were transfected to HEK293 cells by lipofectamine 3000 according to manufacturer’s protocol (Invitrogen, CA, USA). Cells were lysed by RIPA buffer and 20μg of whole cell lysate were used to assess protein expression in Western blot ...
-
bioRxiv - Molecular Biology 2024Quote: ... HEK293 and MS2-tagged HEK293:24xMS2-NEAT1 cells were cultured in Dulbecco’s modified eagle’s medium (DMEM, Gibco) with 10% fetal bovine serum (FBS ...
-
bioRxiv - Microbiology 2019Quote: Human IL-18 and TNFα were measured with the human IL-18 ELISA Kit (MBL, Woburn, Massachusetts) and human TNFα ELISA Kit (ThermoFisher, Waltham, Massachusetts) according to the manufacturers’ instructions using cell culture supernatant at a 1:5 dilution ...
-
bioRxiv - Immunology 2022Quote: ... 1% MEM NEAA and 50 Units/mL recombinant human IL-2 (Myltenyi; #130-097-745) with Dynabeads™ Human T-activator CD3/CD28 (Gibco, #11132D) the same day of the purification (day 0 ...
-
bioRxiv - Microbiology 2021Quote: ... The cells were then stimulated with 20 ng/mL of recombinant human IL-4 (Gibco) and 20 ng/mL of recombinant human IL-13 (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2020Quote: ... One ng/mL recombinant human interleukin (IL)-6 (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), was added to the media of the IL-6 dependent myeloma cell lines INA-6 ...
-
bioRxiv - Immunology 2022Quote: ... 2-mercaptoethanol (RP-10) and 10 ng/ml recombinant human IL-2 (Thermo Fisher Scientific) for 18-22 hours ...
-
bioRxiv - Bioengineering 2021Quote: The HHH peptide (Cys-biotin-PEG2-Gly-His-His-His-Gly(COOH)) was purchased from Thermo Fisher. It was 84% pure as determined by mass spectrometry ...
-
bioRxiv - Molecular Biology 2024Quote: The cDNA sequence of human Sph1 was subcloned from the pcDNA3.1/V5-His-TOPO expression vector (Invitrogen, USA) 62 into the Venus-BiFC plasmids previously described 37 ...
-
bioRxiv - Molecular Biology 2021Quote: ... ELMSAN1 TRERF1 DKO HEK293 cells were generated from wild-type Flp-In T-REx HEK293 cells (Invitrogen, R78007) by the Center for Advanced Genome Engineering (CAGE ...
-
bioRxiv - Cancer Biology 2022Quote: ... (FBS HI; Invitrogen) and 1% Glutamax (Invitrogen) ...
-
bioRxiv - Microbiology 2022Quote: ... anti-His (Invitrogen), or anti-FLAG (Sigma ...
-
bioRxiv - Microbiology 2021Quote: ... IL-22 (IL-22JOP, Thermofisher), IFNγ (XMG1.2 ...